Bioraction chip, centrifugal microfluidic system and amplification method

Abstract

The application discloses a biological reaction chip, a centrifugal micro-fluid system and an amplification method, and relates to the technical field of biological reaction chips.The biological reaction chip comprises a chip main body, a sample storage cavity, a pre-amplification cavity and a detection cavity are sequentially arranged on a first surface of the chip main body and are in communication with each other, a reaction liquid storage cavity and a reaction liquid distribution cavity are sequentially arranged on a second surface of the chip main body and are in communication with each other, the reaction liquid distribution cavity and the detection cavity are in communication through a first through hole arranged on the chip main body, a first sealing body seals the first surface of the chip main body, and a second sealing body seals the second surface of the chip main body.The biological reaction chip realizes twice amplification through centrifugal action in a closed environment, and the risk of pollution is avoided.In addition, the first-step nucleic acid amplification and the second-step nucleic acid amplification are independently carried out in separate cavities, and problems such as cross interference between primers or probes of different amplification targets existing in a multiple amplification method are eliminated.

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