一种生产L-精氨酸的基因工程菌及其应用
By knocking out specific genes in Escherichia coli and introducing arginine biosynthesis-related gene clusters and the RpoB P564T mutant, the L-arginine synthesis pathway was optimized, solving the problem of insufficient production performance of existing strains and achieving efficient production of L-arginine.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NINGXIA EPPEN BIOTECH CO LTD
- Filing Date
- 2022-12-14
- Publication Date
- 2026-07-17
AI Technical Summary
The production performance of existing L-arginine-producing strains has not yet reached the economical and efficient industrial requirements, and further exploration of metabolic targets is needed to improve strain performance.
Using Escherichia coli as the host, we optimized the arginine synthesis pathway and metabolic network of E. coli by knocking out the genes encoding arginine decarboxylase, decarboxylase and succinyltransferase, heterologously overexpressing the arginine biosynthesis-related gene cluster and carbamoyl phosphate synthase gene, and introducing the RpoB P564T mutant.
Stable and efficient production of L-arginine was achieved, increasing yield and productivity, and significantly improving the L-arginine production capacity of Escherichia coli.
Smart Images
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