Methods for determining the detection accuracy of digital PCR at low copy numbers

Abstract

This invention provides a method for determining the detection accuracy of digital PCR at low copy numbers, relating to the field of PCR detection technology. The method includes: acquiring target parameters, which include a specified gene copy number, a specified theoretical droplet number, and a specified probability, wherein the specified gene copy number is less than a preset threshold; and determining the target detection accuracy corresponding to the target parameters based on a pre-established statistical model for digital PCR at low copy numbers. The statistical model for digital PCR at low copy numbers is based on binomial distribution theory. This method theoretically determines the detection accuracy of digital PCR at low copy numbers, alleviating the problem of large detection accuracy errors when using existing methods. It is easy to understand and implement.

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