Microbial strain hatching system for river regulation

Abstract

The present application aims at providing a microbial strain hatching system for river management, comprising the following components arranged in a shell: a raw material feeding module, a strain hatcher, a strain constant hatcher, a fermentation gas supply module, a secondary expansion module and a control module; the microbial strain hatching system for river management provided by the present application adopts air-lift circulation fermentation process in the strain hatcher, and controls the dissolved oxygen in the fermentation process by controlling the gas internal circulation and external circulation gas intake, thereby solving the problems of high stirring power consumption and poor nutrient mixing of the original paddle type fermentation tank.

Description

Technical Field

[0001] This invention relates to a microbial incubation system, and more particularly to a microbial incubation system suitable for river management, belonging to the field of environmental protection equipment technology. Background Technology

[0002] With the development of urbanization, the urban water environment is increasingly affected by the urbanization process. More and more pollutants are discharged into rivers. After a large amount of organic pollutants enter the water body, they damage the water body's own degradation and purification system. The odorous substances such as humus produced during the decomposition process are deposited at the bottom of the water body, producing volatile and irritating odors and gases. Heavy metal pollutants discharged into the water body form sulfides with sulfur in the water, forming a large number of charged colloidal black suspended particles, which causes the water body to appear black and smelly.

[0003] Currently, the main methods for solving these problems include physicochemical methods, physical-mechanical methods, and biological methods. Physicochemical methods are temporary solutions that only address the symptoms, not the root cause. Physical-mechanical methods often involve dredging bottom sediment, but this lacks continuity, requiring dredging periodically, which is costly and labor-intensive. Microbial remediation technology for water bodies involves adding microbial agents to appropriate locations within the water body, combined with techniques such as water aeration and microbial fixation, to enhance the degradation capacity of native or exogenous microorganisms for pollutants, thereby gradually restoring water quality. This technology does not require the construction of structures within the water body; it can remove river pollution "on-site," reduce bottom sediment, and quickly resolve prominent black and odorous water problems, and has been widely applied in the treatment of black and odorous water bodies.

[0004] Currently, the main methods for using microbial agents include direct addition, dissolution and activation addition, and secondary expansion cultivation. Due to the high price and low microbial activity of these agents, multiple activation and expansion cultivation processes are generally required on-site before addition to increase the number of effective microorganisms and improve their activity. The expansion cultivation method mostly employs a secondary expansion cultivation approach. The specific steps involve taking a certain amount of water to be treated, adding the agent to the mixture, and providing other environmental conditions for microbial growth. This allows the added microorganisms and native microorganisms to grow in large quantities in the mixture. Once a certain number have been reached, the mixture is returned to the contaminated water body.

[0005] Currently, there are few dedicated devices for this method, and during use, there are often problems such as high equipment power consumption, high manual operation intensity, low nutrient utilization rate of strains, poor fermentation effect, and low amplification rate. After a period of use, most of the equipment becomes a decoration, wasting a lot of investment costs. Summary of the Invention

[0006] The purpose of this invention is to address the shortcomings of the aforementioned microbial incubation equipment by providing a microbial incubation system for river management.

[0007] A microbial incubation system for river management includes the following components housed within a shell: a raw material feeding module, an incubator, a chemostat, a fermentation gas supply module, a secondary expansion module, and a control module. The raw material feeding module includes a carbon source tank, an alkali tank, and a microbial activation tank. The inlets of the carbon source tank, alkali tank, and microbial activation tank are respectively connected to the process water pipeline. The outlet of the carbon source tank is connected to the inlet of the microbial incubator after passing through a carbon source metering pump. The outlet of the alkali tank is connected to the inlet of the microbial incubator after passing through an alkali metering pump. The top of the microbial activation tank is connected to a microbial feeder. A stirring device is provided in the middle of the microbial activation tank. The outlet at the bottom of the microbial activation tank is connected to the inlet of the microbial incubator after passing through a microbial metering pump. The incubator is equipped with a jacketed heating device on the outside and a feed inlet in the upper middle part of the side. The incubator is also equipped with an air inlet pipe on the side, which extends to the bottom of the incubator and forms a ring-shaped air distribution branch pipe. The other end of the air inlet pipe is connected to the fermentation gas supply module. The incubator is equipped with an online pH meter, an online dissolved oxygen meter, and an online thermometer. The top of the incubator is equipped with an exhaust port, a breathing port, and a hand hole. The inlet at the bottom of the culture constant is connected to the outlet at the bottom of the culture incubator. The culture constant has a microbial nutrient layer, a fixed bed and a carrier layer from bottom to top. The microbial nutrient layer is composed of maifan stone activated balls. The fixed bed is filled with polyurethane filler. The carrier layer is filled with cross-linked sodium alginate gel balls. The outlet on the upper side of the culture constant is connected to the inlet of the culture incubator through a process water pipeline. The fermentation gas supply module includes a vortex mixer fan. The exhaust port of the vortex mixer fan is connected to the air inlet pipe of the incubator. The air inlet of the vortex mixer fan is connected to the exhaust port at the top of the incubator and a sterile air source, respectively. The secondary expansion module includes an expansion box, a submersible mixer, a microporous aerator, an air pump, and packing material. The upper side of the expansion box is provided with an inlet and an outlet. The inlet is connected to the outlet of the incubator and a river water source, respectively. The outlet is connected to the river. The submersible mixer and the microporous aerator are located at the bottom of the expansion box. The microporous aerator is connected to the outlet of the air pump located outside the expansion box. The middle part of the expansion box is filled with packing material. Solenoid valves are installed at the points where the inlets of the carbon source tank, alkali tank, and inoculum activation tank are connected to the process water pipeline; solenoid valves are installed at the points where the air inlet of the vortex mixer is connected to the exhaust port at the top of the inoculum incubator; solenoid valves are installed at the points where the air inlet of the vortex mixer is connected to the sterile air source; and solenoid valves are installed at the inlet of the inoculum chemotherapeutic device. The carbon source metering pump, alkali metering pump, bacterial culture metering pump, jacketed heating device, online pH meter, online dissolved oxygen meter, online thermometer, cyclone fan, and solenoid valve are all communicatively connected to the control module.

[0008] Preferably, the strain refers to Paracandidimonas soli、 A combination of one or more of Paracoccus and Bacillus subtilis, wherein... Paracandidimonas soli The strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, with accession number CGMCC No. 24445 and deposit date of February 28, 2022. Its 16S rDNA sequence is shown in SEQ ID No:1.

[0009] Preferably, both the inoculum activation tank and the inoculum incubator are equipped with level gauges, and the level gauges are communicatively connected to the control module.

[0010] Preferably, a circulation pump is installed on the pipeline connecting the inlet at the bottom of the microbial chemotherapeutic device and the outlet of the microbial incubator.

[0011] Preferably, an air filter and a gas flow meter are installed on the pipeline between the sterile air source and the vortex mixer, and the gas flow meter is communicatively connected to the control module.

[0012] Preferably, the process water pipeline is equipped with an ultraviolet disinfection facility.

[0013] Preferably, the side of the incubator for microbial culture is provided with a long strip-shaped viewing window.

[0014] Preferably, the incubator for microbial culture is provided with an airlift guide tube in the middle.

[0015] The maifan stone activated balls used in this invention are made from maifan stone powder, quartz, plagioclase and kaolin and other natural mineral materials, through batching, ball milling and high-temperature sintering. The maifan stone balls contain a variety of macro and micro elements that are beneficial to the growth and development of organisms, as well as rare earth elements and trace amino acids that are known as animal growth regulators. These elements have a variety of characteristics such as good solubility, mineralization, biological activity, adsorption, and bidirectional regulation of elements and pH value in water.

[0016] Polyurethane filler is obtained by foaming polyurethane foam, sodium sulfite, ferrous salt, hydroxylamine, polyvinyl alcohol, sodium alginate and calcium chloride. Polyurethane foam has a huge specific surface area, which can effectively retain bacteria. It can also maintain the reducing conditions on the surface of the filler through the slow release of sodium sulfite and ferrous ions, thus promoting the growth and reproduction of bacteria that remove total nitrogen.

[0017] Cross-linked sodium alginate gel balls are made by adding calcium chloride as a gelling agent and glutaraldehyde as a cross-linking agent to a sodium alginate solution, and then placing specific bacterial strains (…) into the gel. Paracandidimonas soli It is formed by immobilizing one or more of the following bacteria (such as *Paracoccus*, *Bacillus subtilis*, and *Bacillus subtilis*) and a specific enzyme preparation (catalase or polyphenol oxidase). The preparation process can refer to the following method: Take a specific bacterial strain (… Paracandidimonas soli Wet bacterial cells (one or more of *Paracococcus*, *Bacillus subtilis*, and a combination thereof) are mixed thoroughly with a 3-5 wt% sodium alginate solution. Then, a 2-6 wt% calcium chloride solution is added dropwise to form uniform spheres with a diameter of 2-3 mm. Calcification is allowed for 3-5 hours, followed by the addition of phosphate buffer and overnight equilibration. After removing the supernatant, 3-8 mg / mL of catalase or polyphenol oxidase is added, and the mixture is shaken at 30-40°C for 12-24 hours to allow the sodium alginate gel spheres to fully adsorb the catalase or polyphenol oxidase. Finally, a 4-6% glutaraldehyde solution is added, and the reaction continues for 8-12 hours to cross-link and immobilize the catalase or polyphenol oxidase onto the sodium alginate gel spheres, forming cross-linked sodium alginate gel spheres. These gel spheres effectively increase bacterial concentration, reduce the amount of bacteria required, improve system treatment efficiency and adaptability, and rapidly degrade target pollutants.

[0018] Compared with the prior art, the present invention has the following beneficial effects: (1) The microbial incubation system for river management provided by the present invention adopts the airlift circulation fermentation process in the incubator. The dissolved oxygen in the fermentation process is controlled by controlling the gas internal circulation and external circulation air intake, which solves the problems of high stirring power consumption and poor nutrient mixing of the original paddle fermenter. (2) The strain incubation system provided by the present invention adds a strain recirculation chemotherapeutic device on the basis of the original strain incubation tank. The microbial nutrient layer, fixed bed and carrier layer inside the strain chemotherapeutic device can efficiently promote the reproduction and growth of strains, meet the fixation and continuous release capacity of efficient microorganisms, solve the problem of large strain dosage, and at the same time, the system's resistance to shock load and resistance to toxicity inhibition are greatly improved. (3) The strain incubation system of the present invention adopts continuous flow perfusion culture combined with secondary fermentation expansion process. Compared with the traditional batch and fed batch culture methods, continuous perfusion culture can express more products with smaller equipment, and can also effectively improve product quality. In addition, the feeding nutrients are continuously added and harmful metabolites are removed in time, so that the cells can maintain high-density culture and survival rate for a long time. (4) Combined with the secondary fermentation expansion process, the efficient use of nutrients is ensured, the strain activity is high, and the yield is large; (5) The secondary fermentation process uses the organic matter in the low-concentration river water mixture as a substrate, which allows the cultured strains to quickly adapt to the water environment and avoids the impact of high-concentration culture medium on the water quality of rivers and lakes. It has the advantages of low dosage of bacterial agent, good treatment effect and fast results. (6) By adopting the air-lift circulation fermentation process + strain chemostat + continuous flow perfusion culture combined with secondary fermentation expansion process, the strain can be truly incubated in situ, and the strain can be expanded and domesticated at an exponential level. Attached Figure Description

[0019] Figure 1 This is a plan view of the microbial strain incubation system of the present invention.

[0020] Figure 2 This is a flowchart of the microbial strain incubation system of the present invention.

[0021] Figure 3 This is a schematic diagram of the internal structure of a microbial incubator.

[0022] Figure 1-3 The components are as follows: 1. Carbon source tank; 2. Alkali tank; 3. Microbial activation tank; 4. Carbon source metering pump; 5. Alkali metering pump; 6. Microbial feeder; 7. Stirring device; 8. Microbial metering pump; 9. Microbial incubator; 10. Jacketed heating device; 11. Air inlet pipe; 12. Annular air distribution branch pipe; 13. Online pH meter; 14. Online dissolved oxygen meter; 15. Online thermometer; 16. Exhaust port; 17. Breathing port; 18. Hand hole; 19. Microbial chemostat; 20. Microbial nutrient layer; 21. Fixed bed; 22. Carrier layer; 23. Swirl mixer; 24. Propagation box; 25. Submersible mixer; 26. Microporous aerator; 27. Air pump; 28. Packing material; 29. ​​Solenoid valve; 30. Control module; 31. Ultraviolet disinfection facility; 32. Visual window; 33. Airlift guide tube. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0024] A microbial incubation system for river management, such as Figure 1-3 As shown, it includes the following components housed within the shell: raw material feeding module, incubator, chemostat, fermentation gas supply module, secondary expansion module, and control module. The shell dimensions are 3500*2200*2300mm. The raw material feeding module includes a carbon source tank 1, an alkali tank 2, and a microbial activation tank 3. The inlets of the carbon source tank, alkali tank, and microbial activation tank are connected to the process clean water pipeline. The outlet of the carbon source tank is connected to the inlet of the microbial incubator 9 via a carbon source metering pump 4. The outlet of the alkali tank is connected to the inlet of the microbial incubator 9 via an alkali metering pump 5. The top of the microbial activation tank is connected to the microbial feeder 6. A stirring device 7 is provided in the middle of the microbial activation tank. The outlet at the bottom of the microbial activation tank is connected to the inlet of the microbial incubator via a microbial metering pump 8. 10% liquid microbial agent is automatically prepared and added to the microbial incubator at the set feeding frequency. The incubator has dimensions of φ1000*1600mm. The outer side of the incubator is equipped with a jacketed heating device 10, and the upper middle part of the side is equipped with a feed inlet. The side of the incubator is equipped with an air inlet pipe 11, which extends to the bottom of the incubator to form a ring-shaped air distribution branch pipe 12. The other end of the air inlet pipe is connected to the fermentation gas supply module. The interior of the incubator is equipped with an online pH meter 13, an online dissolved oxygen meter 14, and an online thermometer 15. The top of the incubator is equipped with an exhaust port 16, a breathing port 17, and a hand hole 18. The inlet at the bottom of the culture constant 19 is connected to the outlet at the bottom of the culture incubator. The culture constant 19 contains a microbial nutrient layer 20, a fixed bed 21 and a carrier layer 22 from bottom to top. The microbial nutrient layer is composed of maifan stone activated balls. The fixed bed is filled with polyurethane filler. The carrier layer is filled with cross-linked sodium alginate gel balls. The outlet on the side of the culture constant 19 is connected to the inlet of the culture incubator through a process water pipeline. The fermentation gas supply module includes a vortex mixer 23. The exhaust port of the vortex mixer is connected to the air inlet pipe of the incubator via a pipeline. The air inlet of the vortex mixer is connected to the exhaust port at the top of the incubator and a sterile air source, respectively. The secondary expansion module includes an expansion box 24, a submersible mixer 25, a microporous aerator head 26, an air pump 27, and packing material 28. The expansion box measures 2200*1000*2300mm. The upper and middle sides of the expansion box are equipped with an inlet and an outlet, respectively. The inlet is connected to the outlet of the incubator and the river water source, respectively. The outlet leads to the river. The submersible mixer and the microporous aerator head are located at the bottom of the expansion box. The microporous aerator head is connected to the outlet of the air pump located outside the expansion box. The middle of the expansion box is filled with packing material. Solenoid valves 29 are installed at the points where the inlets of the carbon source tank, alkali tank, and inoculum activation tank are connected to the process clean water pipeline. Solenoid valves are also installed at the points where the air inlet of the vortex mixer is connected to the exhaust port at the top of the inoculum incubator. Solenoid valves are also installed at the points where the air inlet of the vortex mixer is connected to the sterile air source. Solenoid valves are also installed at the inlet of the inoculum chemotherapeutic device. The carbon source metering pump, alkali metering pump, bacterial culture metering pump, jacketed heating device, online pH meter, online dissolved oxygen meter, online thermometer, cyclone fan and solenoid valve are all connected to the control module 30 for communication.

[0025] Preferably, both the inoculum activation tank and the inoculum incubator are equipped with level gauges, which are communicatively connected to the control module.

[0026] Preferably, a circulation pump is installed on the pipeline connecting the feed inlet at the bottom of the culture chemist and the discharge outlet of the culture incubator.

[0027] Preferably, an air filter and a gas flow meter are installed on the pipeline between the sterile air source and the vortex mixer, and the gas flow meter is communicatively connected to the control module.

[0028] Preferably, the process water pipeline is equipped with an ultraviolet disinfection facility 31.

[0029] Preferably, the side of the incubator for microbial culture is provided with a long strip-shaped viewing window 32.

[0030] Preferably, the incubator for microbial culture is provided with an airlift guide tube 33 in the middle.

[0031] The working process and principle of the microbial strain incubation system of the present invention are described below: 1. Nutritional supplements The raw material addition module has three nutrient solution storage tanks, which store alkali solution, special nutrient carbon source and microbial activation solution respectively. Each tank is equipped with a metering pump to add the corresponding liquid to the microbial incubator. In automatic operation, the alkali solution metering pump will automatically add alkali solution according to the pH change of the incubator, and the nutrient carbon source and microbial activation solution will also be added automatically according to the set addition frequency.

[0032] A 10wt% sodium carbonate solution is added to the alkali tank. Before addition, the solution is dissolved and stirred thoroughly with an appropriate amount of water. The carbon source tank contains a dedicated nutrient carbon source, including organic acids, buffers, enzymes, cytokinins, vitamins, and trace elements. Before addition, the carbon source is dissolved and stirred thoroughly with an appropriate amount of water. Solid microbial agents are added to the inoculum feeder, automatically preparing a 10% concentration of microbial solution, which is then transferred to the inoculum activation tank for activation. After activation, the activated inoculum solution is added to the inoculum incubator via a metering pump.

[0033] 2. Set the fermentation conditions for the incubator: temperature 25-35℃, pH=6-7.5, DO≥2mg / L, fermentation time 2-8 hours, system starts automatically. During the cultivation process, the system monitors the temperature, dissolved oxygen, and pH of the fermenter in real time through monitoring probes and feeds this information back to the control module. When the temperature, dissolved oxygen, and pH exceed or fall below the optimal values, the control module will selectively activate the jacketed heating device on the outside of the incubator, the air inlet solenoid valve, and the alkali metering pump to adjust the cultivation temperature, dissolved oxygen, and pH of the target microorganisms to the optimal range.

[0034] 3. When using the system, transport it to the riverbank, place the inlet submersible pump into the river, connect the incubator, alkali tank, carbon source tank, and incubator activation tank to the process water, and connect the control module to the external power supply. The entire incubator system can then be used effectively.

[0035] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solutions of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A microbial incubation system for river management, characterized in that, It includes the following components located inside the shell: raw material feeding module, incubator, chemostat, fermentation gas supply module, secondary expansion module and control module; The raw material feeding module includes a carbon source tank, an alkali tank, and a microbial activation tank. The inlets of the carbon source tank, alkali tank, and microbial activation tank are respectively connected to the process water pipeline. The outlet of the carbon source tank is connected to the inlet of the microbial incubator after passing through a carbon source metering pump. The outlet of the alkali tank is connected to the inlet of the microbial incubator after passing through an alkali metering pump. The top of the microbial activation tank is connected to a microbial feeder. A stirring device is provided in the middle of the microbial activation tank. The outlet at the bottom of the microbial activation tank is connected to the inlet of the microbial incubator after passing through a microbial metering pump. The incubator is equipped with a jacketed heating device on the outside and a feed inlet in the upper middle part of the side. The incubator is also equipped with an air inlet pipe on the side, which extends to the bottom of the incubator and forms a ring-shaped air distribution branch pipe. The other end of the air inlet pipe is connected to the fermentation gas supply module. The incubator is equipped with an online pH meter, an online dissolved oxygen meter, and an online thermometer. The top of the incubator is equipped with an exhaust port, a breathing port, and a hand hole. The inlet at the bottom of the incubator is connected to the outlet at the bottom of the incubator. The incubator contains a microbial nutrient layer, a fixed bed, and a carrier layer from bottom to top. The microbial nutrient layer is composed of maifan stone activated balls. The fixed bed is filled with polyurethane filler. The carrier layer is filled with cross-linked sodium alginate gel balls. The outlet on the upper side of the incubator is connected to the inlet of the incubator through a process water pipeline. A circulation pump is installed on the pipeline connecting the inlet at the bottom of the incubator and the outlet of the incubator. The fermentation gas supply module includes a vortex mixer fan. The exhaust port of the vortex mixer fan is connected to the air inlet pipe of the incubator. The air inlet of the vortex mixer fan is connected to the exhaust port at the top of the incubator and a sterile air source, respectively. The secondary expansion module includes an expansion box, a submersible mixer, a microporous aerator, an air pump, and packing material. The upper side of the expansion box is provided with an inlet and an outlet. The inlet is connected to the outlet of the incubator and a river water source, respectively. The outlet is connected to the river. The submersible mixer and the microporous aerator are located at the bottom of the expansion box. The microporous aerator is connected to the outlet of the air pump located outside the expansion box. The middle part of the expansion box is filled with packing material. Solenoid valves are installed at the points where the inlets of the carbon source tank, alkali tank, and inoculum activation tank are connected to the process water pipeline; solenoid valves are installed at the points where the air inlet of the vortex mixer is connected to the exhaust port at the top of the inoculum incubator; solenoid valves are installed at the points where the air inlet of the vortex mixer is connected to the sterile air source; and solenoid valves are installed at the inlet of the inoculum chemotherapeutic device. The carbon source metering pump, alkali metering pump, bacterial inoculum metering pump, jacketed heating device, online pH meter, online dissolved oxygen meter, online thermometer, vortex mixer and solenoid valve are all communicatively connected to the control module; The cross-linked sodium alginate gel ball is formed by immobilizing the bacterial strain and enzyme preparation by adding calcium chloride gelling agent and glutaraldehyde cross-linking agent to sodium alginate solution as a carrier. The enzyme preparation is catalase or polyphenol oxidase. The strain includes Paracandidimonas soli , wherein Paracandidimonas soli The strain is deposited at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, with accession number CGMCC No. 24445.

2. The microbial strain incubation system according to claim 1, characterized in that, The strains also include one or two of Paracoccus and Bacillus subtilis.

3. The microbial strain incubation system according to claim 1 or 2, characterized in that, Both the inoculum activation tank and the inoculum incubator are equipped with level gauges, which are communicatively connected to the control module.

4. The microbial strain incubation system according to claim 1 or 2, characterized in that, An air filter and a gas flow meter are installed on the pipeline between the sterile air source and the vortex mixer. The gas flow meter is communicatively connected to the control module.

5. The microbial strain incubation system according to claim 3, characterized in that, An air filter and a gas flow meter are installed on the pipeline between the sterile air source and the vortex mixer. The gas flow meter is communicatively connected to the control module.

6. The microbial strain incubation system according to any one of claims 1, 2, or 5, characterized in that, The process water pipeline is equipped with ultraviolet disinfection facilities.

7. The microbial strain incubation system according to claim 3, characterized in that, The process water pipeline is equipped with ultraviolet disinfection facilities.

8. The microbial strain incubation system according to claim 4, characterized in that, The process water pipeline is equipped with ultraviolet disinfection facilities.

9. The microbial strain incubation system according to any one of claims 1, 2, or 5, characterized in that, The incubator for microbial culture has a long, narrow viewing window on its side.

10. The microbial strain incubation system according to any one of claims 1, 2, or 5, characterized in that, The incubator for microbial culture is equipped with an airlift guide tube in the middle.

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