A Bol_InD1 molecular marker for identifying the bolting resistance trait of carrot and its application

Through the development of Indel molecular labeling technology, using PCR amplification and sequencing to identify specific DNA fragments in the carrot genome, it solves the problem of difficulty in the existing technology to quickly identify carrot -resistant traits. The carrot breeding process.

CN115852029BActive Publication Date: 2025-01-28INSTITUTE OF VEGETABLES & FLOWERS CHINESE ACADEMY OF AGRICULTURAL SCIENCES +1
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Patent Information

Application Number
CN202211382665.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-11-07
Publication Date
2025-01-28
Estimated Expiration
2042-11-07

AI Technical Summary

Technical Problem

It is difficult for the prior art to quickly identify the bolting resistance traits of carrots, resulting in serious early bolting in carrot planting, which harms the interests of growers.

Method used

A method based on the INDEL molecular labeling, through PCR amplification and sequencing technology, to identify whether the carrot genome contains specific DNA fragments (BOL_IND1-7bp) to quickly identify the pumping traits of carrots.

Benefits of technology

This method is accurate and stable, the detection is simple and convenient, the cost is inefficient, and it can quickly identify the pumping traits of carrots and help to speed up the breeding process of carrots.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention discloses an InDel molecular marker for identifying a carrot bolting resistance trait and its application. The present invention specifically discloses an application of an InDel molecular marker for a carrot bolting resistance trait or a substance for detecting the InDel molecular marker for the carrot bolting resistance trait in identifying or assisting in identifying carrot bolting, wherein the carrot bolting resistance InDel molecular marker is a DNA fragment with a nucleotide sequence of 118-124th positions of SEQ ID No.1. The present invention designs primers for the InDel molecular marker and provides a method for identifying or assisting in identifying a carrot bolting resistance trait. The InDel molecular marker for the carrot bolting resistance trait of the present invention has high accuracy and good stability, is simple and convenient to detect, is economical and efficient, can accelerate the carrot breeding process, and is widely used in aspects such as carrot germplasm resource analysis and molecular-assisted genetic breeding.
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