Illumination system and microscope

Abstract

This invention relates to an illumination system and a microscope. The illumination system includes a standard light source, an illumination light source, a beam splitter, and a lens assembly. The lens assembly is disposed on the light-emitting side of the beam splitter. The beam splitter can receive at least a portion of the light emitted from the standard light source and the illumination light source, and can direct at least a portion of the light emitted from the standard light source and the illumination light source toward the lens assembly. The lens assembly is used to direct the light emitted from the beam splitter toward the sample. The above illumination system features simple switching between the illumination light source and the standard light source, making it convenient to use.

Description

Technical Field

[0001] This invention relates to the field of microscopic imaging technology, and in particular to an illumination system and a microscope. Background Technology

[0002] Currently, in the biomedical field, examining the microstructures of biological tissues typically requires acquiring images of the microstructures and specific spectral information to determine changes in their content and morphology. Imaging microstructures using a microscope usually requires illumination, while obtaining specific spectral information typically necessitates spectral calibration using a standard light source. However, in these technologies, the illumination sources for microscopes and standard light sources are structurally complex and inconvenient to use. Summary of the Invention

[0003] Therefore, it is necessary to provide an illumination system and microscope to address the problems of complex structures and inconvenience in using standard illumination sources for microscopes.

[0004] An illumination system includes a standard light source, an illumination light source, a beam splitter, and a lens assembly. The lens assembly is disposed on the light-emitting side of the beam splitter. The beam splitter is capable of receiving at least a portion of the light emitted from the standard light source and the illumination light source, and is capable of directing at least a portion of the light emitted from the standard light source and the illumination light source toward the lens assembly. The lens assembly is used to direct the light emitted from the beam splitter toward a sample.

[0005] In one embodiment, the light emitted from the standard light source and the illumination light source can be incident on the beam splitter from different sides of the beam splitter, and the beam splitter can reflect the light emitted from the standard light source and transmit the light emitted from the illumination light source.

[0006] In one embodiment, the beam splitter has a beam splitting surface that is inclined to the optical axis of the lens assembly. Light emitted from the illumination source is incident on the beam splitter from the side of the beam splitting surface away from the lens assembly, and light emitted from the standard light source is incident on the beam splitter from the side of the beam splitting surface toward the lens assembly. The beam splitting surface can transmit light emitted from the illumination source and reflect light emitted from the standard light source.

[0007] In one embodiment, the beam splitter includes a first right-angle prism and a second right-angle prism, the inclined surfaces of the first right-angle prism and the second right-angle prism abutting each other to form the beam splitting surface, one side of the first right-angle prism facing the lens assembly and perpendicular to the optical axis of the lens assembly, light emitted from the standard light source entering the beam splitter from the other side of the first right-angle prism, and light emitted from the illumination light source entering the beam splitter from the side of the second right-angle prism.

[0008] In one embodiment, the light emitted from the standard light source and the light emitted from the illumination light source are perpendicular to each other on the incident direction of the beam splitter.

[0009] In one embodiment, the lens assembly includes a plurality of coaxially arranged convex lenses.

[0010] In one embodiment, the lighting system further includes a first controller, a light guide element, and a light source interface. The two ends of the light guide element are respectively connected to the first controller and the light source interface. The light outlet of the light source interface is opposite to the beam splitter. The first controller is used to couple the light emitted from the standard light source into the light guide element.

[0011] In one embodiment, the illumination source is fixed to the side of the beam splitter facing away from the lens assembly, and the light-emitting surface of the illumination source faces the beam splitter.

[0012] In one embodiment, the lighting system further includes a second controller, the lighting source having a positive electrode and a negative electrode, the two electrodes of the second controller being electrically connected to the positive electrode and the negative electrode of the lighting source, respectively, to control the lighting source to turn on or off, and / or to control the output power of the lighting source.

[0013] A microscope includes a stage and an illumination system as described in any of the above embodiments, the stage being used to fix a sample and the illumination system being used to illuminate the sample.

[0014] The aforementioned illumination system, with its beam-splitting element and lens assembly, guides the light emitted from both the standard and illumination light sources onto the sample for illumination. When an image of the sample needs to be acquired, simply turn off the standard light source and turn on the illumination light source, allowing the light emitted from the illumination light source to illuminate the sample through the beam-splitting element and lens assembly. Similarly, when spectral information of the sample needs to be acquired, simply turn off the illumination light source and turn on the standard light source. Therefore, this illumination system integrates the illumination and standard light sources into a single system, resulting in a simple structure. Furthermore, switching between the illumination and standard light sources requires no disassembly, replacement, or relocation of the light sources, making it more convenient to use. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the lighting system in some embodiments.

[0016] Figure label:

[0017] 10. Lighting system; 101. Lighting module; 102. Lighting source; 1022. Positive electrode; 1024. Negative electrode; 103. Beam splitter; 1032. Beam splitter surface; 1034. First right-angle prism; 1036. Second right-angle prism; 104. Lens assembly; 1042. Convex lens; 105. First controller; 106. Light guide element; 107. Light source interface; 108. Second controller; 109. Control module; 201. Stage. Detailed Implementation

[0018] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0019] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0020] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0021] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0022] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0023] It should be noted that when an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0024] In related technologies, microscopes use different light sources to acquire images and spectral information of samples. For example, an illumination source is used to acquire images, while a standard source is used to acquire spectral information. In these technologies, the illumination and standard sources are installed independently. When it is necessary to acquire images and spectral information of the sample separately, it is usually necessary to switch between the illumination and standard sources. This can be done by disassembling one source and replacing it with the other, or by transferring the other source to the sample via a mechanical structure. This results in a complex illumination system and inconvenient operation.

[0025] To address the aforementioned problems, this application provides an illumination system and a microscope.

[0026] Please see Figure 1 , Figure 1The diagram illustrates the structure of the illumination system 10 in some embodiments. The illumination system 10 can be applied in a microscope, emitting light to illuminate the sample and facilitate observation. Of course, the illumination system 10 can also be applied to any suitable device that requires illuminating a sample for observation; the sample can be a microstructure such as biological tissue. For example, the illumination system 10 can be applied in a microscope to illuminate the sample by emitting light of different wavelengths to obtain images or spectral information of the sample, thereby facilitating the observation of changes in the content and structural morphology of tissue components caused by lesions in biological tissues.

[0027] Furthermore, in some embodiments, the illumination system 10 includes a standard light source (not shown), an illumination source 102, a beam splitter 103, and a lens assembly 104. The lens assembly 104 is disposed on the light-emitting side of the beam splitter 103, allowing light emitted from both the standard light source and the illumination source 102 to enter the beam splitter 103. The beam splitter 103 can receive light emitted from both the standard light source and the illumination source 102, and can direct the light emitted from both sources toward the lens assembly 104. The lens assembly 104 is used to direct the light emitted from the beam splitter 103 toward the sample to illuminate it.

[0028] It is understood that the aforementioned illumination system 10, with the cooperation of the beam splitter 103 and lens assembly 104, can transmit the light emitted from both the standard light source and the illumination source 102 to the sample for illumination. When it is necessary to acquire an image of the sample, simply turn off the standard light source and turn on the illumination source 102, allowing the light emitted from the illumination source 102 to illuminate the sample through the beam splitter 103 and lens assembly 104. Similarly, when it is necessary to acquire the spectral information of the sample, simply turn off the illumination source 102 and turn on the standard light source, allowing the light emitted from the standard light source to illuminate the sample through the beam splitter 103 and lens assembly 104. Thus, the aforementioned illumination system 10 integrates the illumination source 102 and the standard light source into one system, resulting in a simple structure. Switching between the illumination source 102 and the standard light source can be achieved simply by controlling the on or off of the illumination source 102 and the standard light source, without the need to disassemble, replace, or move the light source, making the illumination system 10 more convenient to use.

[0029] It should be noted that, in this application, describing turning on a light source can be understood as controlling the power supply to the light source through a switch button or software circuit, or exposing the light outlet of the light source through the cooperation of a mechanical structure, so that the light source can emit light. Describing turning off a light source can be understood as controlling the power supply to the light source through a switch button or software circuit, or blocking the light outlet of the light source through the cooperation of a mechanical structure, so that the light source cannot emit light.

[0030] In this application, the emission wavelength and type of the illumination source 102 are not limited, as long as they can meet the illumination requirements of the sample to facilitate image acquisition. For example, in some embodiments, the emission wavelength of the illumination source 102 is between 400nm and 1000nm. The emission wavelength of the illumination source 102 covers a wide wavelength range, which is beneficial for acquiring more comprehensive images of the sample and improving the accuracy of sample observation or detection. Specifically, the illumination source 102 includes, but is not limited to, light-emitting elements such as light-emitting diodes and halogen lamps. Of course, the emission wavelength and type of the illumination source 102 can also be other choices, and can be designed according to the illumination requirements of the sample, as long as the illumination of the sample is met. This application does not specifically limit the emission wavelength range and type of the illumination source 102.

[0031] Understandably, when the sample is illuminated by the illumination source 102, the microscope may also be equipped with a sensor that is compatible with the emission wavelength range of the illumination source 102. The sensor is used to receive the light reflected from the sample in order to obtain an image of the sample.

[0032] In this application, the emission wavelength and type of the standard light source are not limited, as long as they can meet the spectral calibration requirements of the sample to obtain its spectral information. For example, in some embodiments, the emission wavelength of the standard light source can be between 400nm and 1000nm. Of course, the emission wavelength of the standard light source can also have a wider range, such as between 250nm and 1700nm, in order to obtain a wider range of spectral information from the sample and improve the accuracy of the detection. The standard light source includes, but is not limited to, light-emitting elements such as mercury lamps, mercury argon lamps, xenon lamps, and cadmium lamps, and can be specifically designed according to the spectral range requirements of the detection.

[0033] Understandably, when illuminating a sample using a standard light source, one or more suitable elements such as mercury (Hg), neon (Ne), argon (Ar), cadmium (Cd), cesium (Cs), helium (He), and thallium (Tl) can be added to the sample. The spectral information of the sample can be obtained by utilizing the different reactions of these elements to light in different spectral ranges. When illuminating a sample using a standard light source, the microscope may also be equipped with a spectrometer or other device capable of receiving spectral information. The spectrometer is used to receive light reflected or excited by the sample to obtain its spectral information.

[0034] Furthermore, in some embodiments, the light emitted from the standard light source and the illumination light source 102 can be incident on the beam splitter 103 from different sides of the beam splitter 103. The beam splitter 103 can reflect at least a portion of the light emitted from the standard light source and transmit at least a portion of the light emitted from the illumination light source 102, thereby enabling at least a portion of the light emitted from the standard light source and the illumination light source 102 to be emitted toward the lens assembly 104.

[0035] For example, in some embodiments, the beam-splitting element 103 has a beam-splitting surface 1032, which is inclined to the optical axis of the lens assembly 104. Light emitted from the illumination source 102 enters the beam-splitting element 103 from the side of the beam-splitting surface 1032 away from the lens assembly 104, while light emitted from the standard source enters the beam-splitting element 103 from the side of the beam-splitting surface 1032 towards the lens assembly 104. The beam-splitting surface 1032 can transmit the light emitted from the illumination source 102 and reflect the light emitted from the standard source. The light emitted from the illumination source 102 passes through the beam-splitting surface 1032 and then hits the lens assembly 104, while the light emitted from the standard source is reflected by the beam-splitting surface 1032 towards the lens assembly 104. It is understood that, in this application, taking the plane where the beam splitter 1032 is located as an example, this plane divides the space on both sides, and the lens assembly 104 is located in the space on one side. Therefore, the elements located on the same side of the space as the lens assembly 104 can be considered to be located on the side of the beam splitter 1032 facing the lens assembly 104, and the elements located on different sides of the space from the lens assembly 104 can be considered to be located on the side of the beam splitter 1032 facing away from the lens assembly 104.

[0036] In some embodiments, the beam splitter 103 includes a first right-angle prism 1034 and a second right-angle prism 1036. The inclined surfaces of the first right-angle prism 1034 abut each other to form a beam splitting surface 1032, for example, the first right-angle prism 1034 and the second right-angle prism 1036 are glued together at the inclined surfaces. One side of the first right-angle prism 1034 faces the lens assembly 104 and is perpendicular to the optical axis of the lens assembly 104, while the other side is parallel to the optical axis of the lens assembly 104. Light emitted from the standard light source enters the beam splitter 103 from the other side of the first right-angle prism 1034. One side of the second right-angle prism 1036 is perpendicular to the optical axis of the lens assembly 104, while the other side is parallel to the optical axis of the lens assembly 104. Light emitted from the illumination source 102 enters the beam splitter 103 from the side of the second right-angle prism 1036 perpendicular to the optical axis of the lens assembly 104. It should be noted that the cross-section of the right-angle prism is a right-angled triangle. In this application, the surface in the right-angle prism corresponding to the hypotenuse of the right-angled triangle in the cross-section is called the inclined surface of the right-angle prism, and the two surfaces in the right-angle prism corresponding to the two right-angled sides of the right-angled triangle in the cross-section are both called the lateral surfaces of the right-angle prism.

[0037] The specific arrangement of the beam splitter 103 is not limited, as long as it can reflect the light emitted from the standard light source and transmit the light emitted from the illumination source 102, so that light incident on the beam splitter 103 from different sides can all exit towards the lens assembly 104. For example, in some embodiments, the beam splitter 103 is provided with a semi-transparent and semi-reflective film, which is located at the junction of the first right-angle prism 1034 and the second right-angle prism 1036, that is, at the beam splitting surface 1032. The semi-transparent and semi-reflective film can transmit and reflect a portion of the light emitted from the standard light source, and can also transmit and reflect a portion of the light emitted from the illumination source 102. For example, the semi-transparent and semi-reflective film can reflect 40%, 50%, or 60% of the light emitted from the standard light source and can transmit 40%, 50%, or 60% of the light emitted from the illumination source 102, as long as the illumination requirements of the sample are met when either the standard light source or the illumination source 102 emits light. In other embodiments, a selective transmission film layer may also be provided at the beam-splitting surface 1032. The material of the selective transmission film layer can be designed according to the emitted light of the standard light source and the illumination light source 102 to maximize the reflection of the light emitted from the standard light source and the transmission of the light emitted from the illumination light source 102. For example, it can reflect more than 60% of the light emitted from the standard light source and transmit more than 60% of the light emitted from the illumination light source 102 to improve the light utilization efficiency. The design relationship between the standard light source, the illumination light source 102 and the beam-splitting element 103, as well as the specific setting of the beam-splitting element 103, are not limited. For example, the beam-splitting element 103 can also be one or more beam-splitting prisms, as long as the beam-splitting element 103 can emit the light emitted from the standard light source and the illumination light source 102 that are incident from different positions toward the lens assembly 104 to illuminate the sample and meet the illumination requirements of the sample. This application does not specifically limit the setting of the beam-splitting element 103.

[0038] exist Figure 1 In the illustrated embodiment, the incident directions of the light rays emitted from the standard light source and the light rays emitted from the illumination source 102 on the beam splitter 103 are perpendicular to each other. In other words, the central ray of the beam emitted from the standard light source and the central ray of the beam emitted from the illumination source 102 are perpendicular to each other. The beam splitter 1032 can form a 45-degree angle with the optical axis of the lens assembly 104, thereby effectively directing the light rays emitted from both the standard light source and the illumination source 102 toward the lens assembly 104, and simplifying the structural design and layout of the illumination system 10. In other embodiments, the angle of the beam splitter 1032 relative to the optical axis of the lens assembly 104 and the incident angle of the light rays emitted from the standard light source and the illumination source 102 on the beam splitter 103 can be designed in other ways, as long as the beam splitter 103 can direct the light rays emitted from both the standard light source and the illumination source 102 toward the lens assembly 104. Further details are omitted here.

[0039] The lens assembly 104 may include multiple lenses with optical power. The cooperation of these multiple lenses enables the lens assembly 104 to have collimation and / or homogenization functions, allowing the light emitted from both the standard light source and the illumination source 102 to illuminate the sample more uniformly and at a good incident angle, thereby improving the illumination effect and meeting the needs of sample observation. For example, the lens assembly 104 may include multiple convex lenses 1042, which are coaxially arranged. The common axis of the multiple convex lenses 1042 can be understood as the optical axis of the lens assembly 104. Of course, the type and number of lenses in the lens assembly 104 can also be designed in other ways, depending on the light output of the beam splitter 103 and the illumination requirements of the sample. This application does not specifically limit the configuration of the lens assembly 104.

[0040] In some embodiments, the lighting system 10 further includes a first controller 105, a light guide element 106, and a light source interface 107. The light guide element 106 can be an optical fiber, and its two ends are respectively connected to the first controller 105 and the light source interface 107. The first controller 105 can be a controller for a standard light source, and can be used to control the turning on and off of the standard light source. The standard light source can be disposed within or connected to the first controller 105. The first controller 105 is also used to couple the light emitted from the standard light source into the light guide element 106. The light source interface 107 can be docked with the fixed structure of the beam splitter 103, and the light outlet of the light source interface 107 is opposite to the beam splitter 103. The light emitted from the standard light source passes through the light guide element 106 and the light source interface 107 in sequence before exiting towards the beam splitter 103.

[0041] In some embodiments, the lighting source 102 is fixed to the side of the beam splitter 103 facing away from the lens assembly 104, and the light-emitting surface of the lighting source 102 faces the beam splitter 103. In some embodiments, the lighting system 10 further includes a second controller 108, which can be a controller for the lighting source 102, used to control the lighting source 102 to turn on and off. When the lighting source 102 is on, the second controller 108 can also control the output power of the lighting source 102 to meet different lighting needs. For example, the lighting source 102 may have a positive electrode 1022 and a negative electrode 1024, and the two electrodes of the second controller 108 are electrically connected to the positive electrode 1022 and the negative electrode 1024 of the lighting source 102, respectively, to control the lighting source 102.

[0042] It is understood that in the above embodiments, the lighting source 102, the beam splitter 103, and the lens assembly 104 can be integrated into a lighting module 101. For example, the lighting source 102, the beam splitter 103, and the lens assembly 104 can be fixed to each other by a fixed structure. The first controller 105 and the second controller 108 are located outside the lighting module 101. The first controller 105 is connected to the lighting module 101 through the light guide element 106 and the light source interface 107, and the second controller 108 is electrically connected to the lighting source 102 through a wire. This arrangement enables the lighting system 10 to have a high degree of structural integration, simple structural layout and wiring, high space utilization, and convenient control and operation of the lighting source 102 and the standard light source.

[0043] Furthermore, in some embodiments, the lighting system 10 may also include a control module 109. The control module 109 may include components such as an operation panel or a display panel. The control module 109 may be electrically connected to the first controller 105 and the second controller 108 so as to control the first controller 105 and the second controller 108 through software or manual operation of the control panel, thereby controlling the opening and closing of the lighting source 102 and the standard light source.

[0044] This application also provides a microscope (not shown), including a stage 201 and an illumination system 10 as described in any of the above embodiments. The stage 201 is used to fix the sample, and the light-emitting side of the lens assembly 104 can be opposite to the stage 201 to facilitate the illumination system 10 illuminating the sample. In some embodiments, the microscope may also include other components such as a spectrometer and a sensor. The spectrometer can acquire spectral information of the sample when light is emitted from a standard light source, and the sensor can acquire an image of the sample when light is emitted from the illumination source 102.

[0045] Although not shown in the figure, those skilled in the art should understand that the microscope and illumination system 10 of this application may also include other suitable elements to achieve the corresponding functions. The above-mentioned elements may also be replaced by other elements to achieve the same functions, as long as the switching steps between the illumination source 102 and the standard light source can be simplified. This will not be elaborated here.

[0046] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0047] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A lighting system, characterized in that, It includes a standard light source, an illumination light source, a beam splitter, and a lens assembly. The lens assembly is disposed on the light-emitting side of the beam splitter. The beam splitter can receive at least a portion of the light emitted by the standard light source and the illumination light source, and can direct at least a portion of the light emitted by the standard light source and the illumination light source toward the lens assembly. The lens assembly is used to direct the light emitted by the beam splitter toward the sample. The light emission wavelength of the lighting source is between 400nm and 1000nm, and the light emission wavelength of the standard light source is between 250nm and 1700nm. The lighting system further includes a first controller, a light guide element, and a light source interface. The two ends of the light guide element are respectively connected to the first controller and the light source interface. The light outlet of the light source interface is opposite to the beam splitter. The first controller is used to couple the light emitted from the standard light source into the light guide element. The beam-splitting element has a beam-splitting surface that is inclined to the optical axis of the lens assembly. Light emitted from the illumination source enters the beam-splitting element from the side of the beam-splitting surface away from the lens assembly, while light emitted from the standard light source enters the beam-splitting element from the side of the beam-splitting surface toward the lens assembly. The beam-splitting surface can transmit light emitted from the illumination source and reflect light emitted from the standard light source. The beam splitter includes a first right-angle prism and a second right-angle prism. The inclined surfaces of the first right-angle prism and the second right-angle prism abut against each other to form the beam splitting surface. One side of the first right-angle prism faces the lens assembly and is perpendicular to the optical axis of the lens assembly. Light emitted from the standard light source enters the beam splitter from the other side of the first right-angle prism, and light emitted from the illumination light source enters the beam splitter from the side of the second right-angle prism.

2. The lighting system according to claim 1, characterized in that, The beam splitter is provided with a semi-transparent and semi-reflective film, which is located at the junction of the first right-angle prism and the second right-angle prism.

3. The lighting system according to claim 1, characterized in that, The light emitted from the standard light source and the light emitted from the illumination light source are perpendicular to each other on the incident direction of the beam splitter.

4. The lighting system according to claim 1, characterized in that, The lens assembly includes multiple coaxially arranged convex lenses.

5. The lighting system according to claim 1, characterized in that, The illumination source is fixed on the side of the beam splitter facing away from the lens assembly, and the light-emitting surface of the illumination source faces the beam splitter.

6. The lighting system according to claim 1, characterized in that, The lighting system further includes a second controller. The lighting source has a positive electrode and a negative electrode. The two electrodes of the second controller are electrically connected to the positive electrode and the negative electrode of the lighting source, respectively, to control the lighting source to turn on or off, and / or to control the output power of the lighting source.

7. A microscope, characterized in that, It includes a stage and an illumination system as described in any one of claims 1-6, wherein the stage is used to fix the sample and the illumination system is used to illuminate the sample.

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