Tomato SlBBX31 gene, InDel marker and its application

Abstract

The invention discloses a tomato SlBBX31 Gene, InDel marker and application, the SlBBX31 The nucleotide sequence of the gene is shown in SEQ ID NO. 1. The present invention utilizes the gene editing technology CRISPR-Cas9 and the Agrobacterium-mediated stable tomato genetic transformation method to transform SlBBX31 Functional validation was performed by knocking out micro-Tom in tomato. SlBBX31 Gene deletion reduces cold tolerance in tomato plants SlBBX31 Overexpressing plants showed an increased cold tolerance phenotype, indicating SlBBX31 It plays an important role in regulating the response of tomatoes to low temperature stress. SlBBX31 Analysis of gene variation sites found that among different resistant and susceptible materials in the tomato population, SlBBX31 There is a 27 bp insertion / deletion (Indel) variation in the promoter region of the gene. Varieties with the 27 bp base fragment insertion are sensitive to cold stress, while varieties without the 27 bp base fragment insertion have strong resistance to cold stress. A co-dominant InDel marker was developed using this deletion mutation site. The application of this marker will accelerate the breeding process of tomato cold resistance.

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