一种用于检测尿路感染病原体及耐药基因的引物和试剂盒及其应用
By using multiplex fluorescent PCR and in situ lyophilization techniques, specific primer probes were designed to detect urinary tract infection pathogens and drug resistance genes. This solved the problems of narrow detection range, high cost, and cumbersome operation in existing technologies, and achieved rapid, accurate, and convenient urinary tract infection detection, guiding early and precise medication.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- BIOTEKE CORP (WUXI) CO LTD
- Filing Date
- 2023-02-17
- Publication Date
- 2026-07-17
AI Technical Summary
Existing nucleic acid detection technologies for urinary tract infection pathogens have problems such as narrow detection range, high cost, cumbersome operation, and easy to lead to false positive results, and cannot accurately guide medication.
We used multiplex fluorescent PCR combined with in situ freeze-drying technology to design specific primers and probes to detect 19 urinary tract infection pathogens and 12 drug resistance genes. We used a four-channel fluorescent PCR instrument, centrifuge and vortex mixer to simplify the operation and avoid opening the PCR product for analysis.
It enables rapid, accurate, and convenient detection of urinary tract infection pathogens and drug resistance genes, covering more than 90% of common strains, reducing operational difficulty and the risk of false positives, and guiding early precision medication.
Smart Images
Figure CN115992276B_ABST