Preparation process of urokinase and preparation device thereof

Abstract

The application relates to the technical field of biological enzyme extraction, and discloses a preparation process and a preparation device of urokinase, the preparation process comprising the following steps: S1, a urine donor urinates; S2, a urine collecting device stores urine, and a sampling device takes urine; S3, a detection device detects the urine, and first raw liquid and second raw liquid are collected according to the detection result; S3-1, the first raw liquid is centrifuged after acidification, and precipitates are filtered out to obtain first treated liquid, and the first treated liquid is subjected to adsorption, elution and filtration to obtain urokinase crude products; and S3-2, the second raw liquid is subjected to adsorption, elution and filtration to obtain urokinase crude products; the preparation device comprises a urine collecting device for directly collecting urine from the urine donor, a sampling device for obtaining urine, and a detection device for detecting the urine. The preparation process and the preparation device of urokinase can separate contaminated urine and clean urine, and extract urokinase in the separated urine.

Description

Technical Field

[0001] This invention relates to the field of bioenzyme extraction technology, and in particular to a preparation process and apparatus for urokinase. Background Technology

[0002] Urokinase is a highly specific proteolytic enzyme that also has esterase activity. It is non-antigenic, non-toxic, and has no other side effects. Clinically, it can be used to treat diseases such as pulmonary thrombosis, coronary heart disease, cerebral thrombosis, myocardial infarction, vitreous opacity, fundus hemorrhage, intracranial hemorrhage, venous thrombosis, and acute (chronic) glomerulonephritis. It can also be used to treat diseases caused by fibrin deposition and as an adjuvant drug in anticancer treatment.

[0003] Urokinase is an alkaline protease produced by the kidneys and mainly found in the urine of humans and mammals. Existing technologies for extracting urokinase from urine mainly include the following three methods: (1) foaming method: the enzyme solution is foamed by high-speed stirring, then the foam is liquefied, and ammonium sulfate is added to precipitate urokinase; (2) precipitant method: a precipitant is added to the urine to precipitate urokinase, and the urokinase is retained in the precipitate; (3) adsorbent method: this method is the most widely used. An appropriate adsorbent is selected to selectively adsorb urokinase.

[0004] Urine is the main raw material for extracting urokinase. Current technology usually collects a large amount of urine as raw material for urokinase extraction. However, when urine is excreted from the human body, the initial urine will be contaminated with pollutants from the external genitalia, resulting in all the urine collected being contaminated, thus affecting the cleanliness and purity of the extracted urokinase. Summary of the Invention

[0005] The primary objective of this invention is to provide a process for preparing urokinase, which can separate contaminated urine and clean urine, and extract urokinase from the separated urine respectively.

[0006] To achieve the above objectives, the basic solution of the present invention is as follows: a preparation process for urokinase, comprising the following steps:

[0007] S1: The urine donor urinates directly into the urine collection device;

[0008] S2: The urine collection device stores urine according to the time of urination in each urine collection space of the urine collection device, and the sampling device obtains the urine in each urine collection space respectively;

[0009] S3: The detection device detects the urine in each urine collection space and generates a first detection result and a second detection result. The urine collection device discharges the urine in the urine collection space with the first detection result to obtain the first original solution, and discharges the urine in the urine collection space with the second detection result to obtain the second original solution.

[0010] S3-1: After acidifying the first stock solution, centrifuge and filter out the precipitate to obtain the first treatment solution. Add diatomaceous earth to the first treatment solution for adsorption. Elute with elution buffer I and elution buffer II in sequence to obtain a turbid first sample solution. Filter the first sample solution to obtain crude urokinase.

[0011] S3-2: The second original solution was added to modified silica gel for adsorption. After elution with elution buffer III and elution buffer IV in sequence, a turbid second sample solution was obtained. The second sample solution was filtered and freeze-dried to obtain crude urokinase.

[0012] Furthermore, the eluent I is an ammonia solution, and the eluent II is a mixture of an ammonia solution and a sodium chloride solution.

[0013] Furthermore, the eluent III is a mixture of sodium chloride solution and phosphoric acid solution, and the eluent IV is an ammonium sulfate solution.

[0014] A second objective of the present invention is to provide an apparatus for preparing urokinase, comprising a urine collection device, a sampling device, and a detection device;

[0015] The urine collection device is used to directly collect urine from the urine donor, the sampling device is used to obtain urine from each urine collection space of the urine collection device, and the detection device is used to detect urine from each urine collection space.

[0016] The urine collection device includes a urine collection container, a urine catheterization assembly, and a urine storage tower connected in sequence. The urine collection container is used to directly collect urine from the urine donor, and the urine catheterization assembly is used to transport the urine in the urine collection container to the urine storage tower.

[0017] The urine storage tower is provided with multiple partition plates from bottom to top, which divide the urine storage tower into multiple urine collection spaces. The urine collection spaces are used to hold urine, and the urine capacity of each urine collection space is distributed from bottom to top in a manner that first decreases and then increases.

[0018] Furthermore, each urine collection space is equipped with a urine storage component, which includes a catheter ring and a flexible urine storage bag. The catheter ring has a cavity inside.

[0019] The top of the urine storage bag is connected to a catheter ring, and the connection between the catheter ring and the urine storage bag is provided with an injection port for filling the urine storage bag with urine. A first catheter port is opened on the side of the catheter ring, and an injection channel is formed between the first catheter port and the injection port.

[0020] A first catheter valve is installed on the first catheter port, and the first catheter valve is a magnetic suction valve. The outer wall of the urine collection space is provided with a first sliding groove, and the first sliding grooves on the outer wall of adjacent urine collection spaces are independent of each other. The first catheter valve can slide in the first sliding groove of the urine collection space in which it is located.

[0021] The first catheter valve is connected to the urine outlet of the catheter assembly, which can inject urine from the urine collection container into the urine storage bag through the first catheter port and the urine injection port.

[0022] Furthermore, an annular groove is formed on the partition plate, the bottom of the urine storage bag is connected to the adjacent urine collection space through the annular groove, and the top of the urine guide ring is provided with an annular engaging part that can engage with the annular groove.

[0023] When the urine collection bag is filled with urine, it gradually expands until it fills the urine collection space. The urine in the bag, through buoyancy, pushes the catheter ring towards the upper partition plate. When the urine collection bag is full of urine, the catheter ring fits against the upper partition plate and is placed at the top of the urine collection space. The annular locking part of the catheter ring engages with the annular groove to close the annular groove and separate the urine collection bag from the adjacent urine collection space.

[0024] Furthermore, the catheterization assembly includes a catheterization pump, an inlet tube connecting the inlet end of the catheterization pump to the urine collection container, an outlet tube connecting the outlet end of the catheterization pump to the urine collection container, and the outlet of the outlet tube is always higher than the outlet end of the catheterization pump. The outlet tube is a retractable elastic flexible tube.

[0025] The outlet of the urine tube is connected to a second urine-conducting valve, which is slidably connected to the outer wall of the urine storage tower. The outer wall of the urine storage tower is provided with a second sliding groove, in which the second urine-conducting valve slides. The second sliding groove is in contact with and connected to each of the first sliding grooves.

[0026] The second catheter valve is a magnetic valve, and the second catheter valve can be attracted to each of the first catheter valves respectively. When the second catheter valve is attracted to the first catheter valve, the second catheter valve and the first catheter valve are connected to each other.

[0027] When the urine donor urinates directly into the urine collection container, the urine pump guides the urine through the inlet and outlet tubes to the second urine valve. The second urine valve and one of the first urine valves engage with each other, and the urine is injected into the urine storage bag through the first urine valve, the first urine port, and the urine inlet.

[0028] When the urine collection bag is filled with urine, the catheter ring is placed at the top of the urine collection space. The first catheter valve moves with the catheter ring to the end of the first chute. The urine from the outlet tube generates a lateral component impact force and a longitudinal component impact force on the second catheter valve. The longitudinal component impact force drives the second catheter valve to move longitudinally along the second chute and disengage from the currently engaged first catheter valve. At the same time, the first catheter valves in the next first chute engage with each other, so that the catheter pump sequentially fills the urine into the urine collection bags of each urine collection space.

[0029] Furthermore, a second urinary catheter port is provided on the side of the catheter ring, the second urinary catheter port is connected to the urine injection channel, the second urinary catheter port is provided with a urine outlet valve, and the side wall of the urine collection space is provided with a third sliding groove that slides in cooperation with the urine outlet valve; the second urinary catheter port and the first urinary catheter port are respectively provided on both sides of the catheter ring, and the second urinary catheter port is located above the first urinary catheter port.

[0030] Furthermore, the sampling device includes a urine pump and multiple sampling cylinders. An electromagnetic urine valve is fixedly installed on the side wall of the urine collection space and near the top of the urine collection space. One end of the urine pump is connected to the electromagnetic urine valve of each urine collection space, and the other end of the urine pump is connected to multiple sampling cylinders.

[0031] When the urine storage bag is full of urine, the urine catheter ring is placed at the top of the urine collection space. The urine outlet valve of the urine catheter ring and the electromagnetic urine collection valve are in contact and connected. The urine collection pump introduces part of the urine in the urine storage bag into the sampling cylinder.

[0032] Furthermore, the detection device includes a urine analyzer, the detection end of which is connected to each sampling tube and is used to detect the urine in the sampling tube and output urine detection data.

[0033] Compared with existing technologies, the beneficial effects of this solution are:

[0034] 1. Directly collecting urine from the donor ensures the freshness of the urine, thereby effectively ensuring the activity of urokinase in the urine.

[0035] 2. By dividing the interior of the urine storage tower into multiple urine collection spaces, segmented collection of urine is achieved. The urine capacity of each collection space is set to decrease and then increase from bottom to top. Urine is injected into each collection space sequentially from bottom to top. With the help of a detection device to detect the urine in the urine bags of each collection space, it is possible to quickly and accurately distinguish whether the urine in each collection space belongs to the first or second stage of urine, and accurately separate the first and second stage of urine.

[0036] 3. Different extraction processes were used to extract the first and second portions of urine, which effectively improved the cleanliness and purity of the extracted urokinase. Attached Figure Description

[0037] Figure 1 This is a schematic diagram of the overall structure of the urokinase preparation device of the present invention;

[0038] Figure 2 This is a partial cross-sectional view of the urine storage tower of the present invention.

[0039] The reference numerals in the accompanying drawings include: urine collection container 1, urine inlet tube 2, urine catheter pump 3, urine outlet tube 4, urine storage tower 5, urine collection pump 6, sampling cylinder 7, urine analyzer 8, urine storage bag 9, urine collection space 10, second chute 11, first chute 12, second urine catheter valve 13, first urine catheter valve 14, third chute 15, urine outlet valve 16, electromagnetic urine collection valve 17, partition plate 18, annular through groove 19, annular locking part 20, and urine catheter ring 21. Detailed Implementation

[0040] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments:

[0041] Example:

[0042] A device for preparing urokinase, such as Figure 1 As shown, it includes a urine collection device, a sampling device, and a detection device;

[0043] The urine collection device is used to directly collect urine from the urine donor, the sampling device is used to obtain urine from each urine collection space 10 of the urine collection device, and the detection device is used to detect urine from each urine collection space 10.

[0044] The urine collection device includes a urine collection container 1, a urine catheterization assembly, and a urine storage tower 5 connected in sequence. The urine collection container 1 is used to directly collect urine from the urine donor, and the urine catheterization assembly is used to transport the urine in the urine collection container 1 to the urine storage tower 5.

[0045] like Figure 2 As shown, the urine storage tower 5 is provided with multiple partition plates 18 from bottom to top. The partition plates 18 divide the urine storage tower 5 into multiple urine collection spaces 10. The urine collection spaces 10 are used to hold urine, and the urine capacity of each urine collection space 10 is distributed from bottom to top in a manner that first decreases and then increases.

[0046] Each urine collection space 10 is equipped with a urine storage component, which includes a urine guide ring 21 and a flexible urine storage bag 9. Each urine storage bag 9 is equipped with a urine discharge port, and a urine discharge valve (not shown in the figure) is installed on the urine discharge port. The urine guide ring 21 has a cavity inside.

[0047] The urine storage bag 9 can expand to fill the entire urine collection space 10. The top of the urine storage bag 9 is connected to the urine guide ring 21, and the connection between the urine guide ring 21 and the urine storage bag 9 is provided with an injection port for filling the urine storage bag 9 with urine. A first urine guide port is opened on the side of the urine guide ring 21, and a urine injection channel is formed between the first urine guide port and the injection port.

[0048] A first catheter valve 14 is installed on the first catheter port, and the first catheter valve 14 is a magnetic suction valve. The outer wall of the urine collection space 10 is provided with a first slide groove 12, and the first slide groove 12 on the outer wall of adjacent urine collection spaces 10 are independent of each other. The first catheter valve 14 can slide in the first slide groove 12 of the urine collection space 10 in which it is located.

[0049] The first catheter valve 14 is connected to the urine outlet of the catheter assembly, which can inject urine from the urine collection container 1 into the urine storage bag 9 through the first catheter port and the urine injection port.

[0050] An annular groove 19 is formed on the partition plate 18. The bottom of the urine storage bag 9 is connected to the adjacent urine collection space 10 through the annular groove 19. The top of the urine guide ring 21 is provided with an annular engaging part 20 that can engage with the annular groove 19.

[0051] When the urine storage bag 9 is filled with urine, it gradually expands until it fills the urine collection space 10. The urine in the urine storage bag 9 will push the catheter ring 21 to move towards the upper partition plate 18 due to buoyancy. When the urine storage bag 9 is full of urine, the catheter ring 21 fits against the upper partition plate 18 and is placed on the top of the urine collection space 10. The annular locking part 20 of the catheter ring 21 engages with the annular through groove 19 to close the annular through groove 19 and separate the urine storage bag 9 from the adjacent urine collection space 10.

[0052] The catheterization assembly includes a catheterization pump 3, an inlet tube 2 connecting the inlet end of the catheterization pump 3 to the urine collection container 1, and an outlet tube 4 connecting the outlet end of the catheterization pump 3 to the urine collection container 1. The outlet of the outlet tube 4 is always higher than the outlet end of the catheterization pump 3. The outlet tube 4 is a flexible and retractable tube.

[0053] The outlet of the urine tube 4 is connected to a second urine catheter valve 13. The second urine catheter valve 13 is slidably connected to the outer wall of the urine storage tower 5, and the outer wall of the urine storage tower 5 is provided with a second groove 11. The second urine catheter valve 13 slides in the second groove 11. The second groove 11 is in contact with and connected to each of the first grooves 12.

[0054] The second catheter valve 13 is a magnetic valve, and the second catheter valve 13 can be attracted to each of the first catheter valves 14 respectively. When the second catheter valve 13 and the first catheter valve 14 are attracted to each other, the second catheter valve 13 and the first catheter valve 14 are connected to each other.

[0055] When the urine donor urinates directly into the urine collection container 1, the urine pump 3 guides the urine through the inlet tube 2 and the outlet tube 4 to the second urine valve 13. The second urine valve 13 and one of the first urine valves 14 attract each other and inject the urine into the urine storage bag 9 through the first urine valve 14, the first urine port and the urine injection port.

[0056] When the urine collection bag 9 is filled with urine, the catheter ring 21 is placed at the top of the urine collection space 10. The first catheter valve 14 moves with the catheter ring 21 to the end of the first slide 12. The urine from the urine outlet tube 4 generates a lateral component impact force and a longitudinal component impact force on the second catheter valve 13. The longitudinal component impact force drives the second catheter valve 13 to move longitudinally along the second slide 11 and disengage from the currently engaged first catheter valve 14. At the same time, the first catheter valves 14 in the next first slide 12 engage with each other, so that the catheter pump 3 sequentially fills the urine into the urine collection bag 9 of each urine collection space 10.

[0057] The catheterization ring 21 has a second catheterization port on its side, which is connected to the urine injection channel. The second catheterization port is equipped with a urine outlet valve 16, which is a one-way valve. The side wall of the urine collection space 10 is provided with a third sliding groove 15 that slides with the urine outlet valve 16. The second catheterization port and the first catheterization port are respectively located on both sides of the catheterization ring 21, and the second catheterization port is located above the first catheterization port.

[0058] The sampling device includes a urine pump 6 and a sampling cylinder 7. An electromagnetic urine valve 17 is fixedly installed on the side wall of the urine collection space 10 and near the top of the urine collection space 10. One end of the urine pump 6 is connected to the electromagnetic urine valve 17 of each urine collection space 10, and the other end of the urine pump 6 is connected to the sampling cylinder 7.

[0059] When the urine storage bag 9 is filled with urine, the urine catheter ring 21 is placed on top of the urine collection space 10. The urine outlet valve 16 of the urine catheter ring 21 and the electromagnetic urine collection valve 17 are in contact with each other and connected. The urine collection pump 6 introduces part of the urine in the urine storage bag 9 into the sampling cylinder 7.

[0060] The detection device includes a urine analyzer 8, the detection end of which is connected to the sampling cylinder 7 and is used to detect the urine in the sampling cylinder 7 and output urine detection data.

[0061] A process for preparing urokinase includes the following steps:

[0062] S1: The urine donor urinates directly into the urine collection device;

[0063] S2: The urine collection device stores urine in each urine collection space 10 according to the urination time, and the sampling device obtains the urine in each urine collection space 10 respectively.

[0064] S3: The detection device detects the urine in each urine collection space 10 and generates a first detection result and a second detection result. The urine collection device discharges the urine in the urine collection space 10 with the first detection result to obtain the first original liquid, and discharges the urine in the urine collection space 10 with the second detection result to obtain the second original liquid.

[0065] S3-1: After acidifying the first stock solution, centrifuge and filter out the precipitate to obtain the first treatment solution. Add diatomaceous earth to the first treatment solution for adsorption. Elute with elution solution I and elution solution II in sequence to obtain a turbid first sample solution. Elution solution I is an ammonia solution and elution solution II is a mixture of ammonia solution and sodium chloride solution. Filter the first sample solution to obtain crude urokinase.

[0066] S3-2: The second original solution was added to modified silica gel for adsorption. After elution with elution buffer III and elution buffer IV in sequence, a turbid second sample solution was obtained. Elution buffer III was a mixture of sodium chloride solution and phosphoric acid solution, and elution buffer IV was ammonium sulfate solution. The second sample solution was filtered and freeze-dried to obtain crude urokinase.

[0067] The specific implementation method of this solution is as follows:

[0068] When in use, turn on the urine catheter pump 3, and the urine donor urinates directly into the urine collection container 1. As the urine flows into the urine collection container 1, the urine catheter pump 3 guides the urine through the inlet tube 2 and the outlet tube 4 to the second urine catheter valve 13. In the initial state, the second urine catheter valve 13 and the first urine catheter valve 14 on the urine catheter ring 21 in the urine collection space 10 at the bottom of the urine storage tower 5 attract each other and communicate with each other. The urine is injected into the urine storage bag 9 through the second urine catheter valve 13, the first urine catheter port and the urine injection port.

[0069] As urine is gradually filled into the urine storage bag 9, the bag gradually expands until it fills the urine collection space 10. The urine in the bag 9, through buoyancy, pushes the catheter ring 21 toward the direction of the upper partition plate 18. When the urine storage bag 9 is full of urine, the catheter ring 21 adheres to the upper partition plate 18 and is placed on top of the urine collection space 10. The annular locking part 20 of the catheter ring 21 engages with the annular through groove 19 to close the annular through groove 19 and separate the urine storage bag 9 from the adjacent urine collection space 10.

[0070] Simultaneously, when the urine storage bag 9 is filled with urine, the catheter ring 21 is placed at the top of the urine collection space 10. The first catheter valve 14 moves with the catheter ring 21 to the end of the first slide 12. The urine from the urine outlet tube 4 generates a lateral component impact force and a longitudinal component impact force on the second catheter valve 13. The longitudinal component impact force drives the second catheter valve 13 to move longitudinally along the second slide 11 and disengage from the currently engaged first catheter valve 14. At the same time, the first catheter valves 14 in the next first slide 12 engage with each other, so that the catheter pump 3 sequentially injects urine into the urine storage bag 9 of each urine collection space 10.

[0071] When the urine storage bag 9 is filled with urine, the urine catheter ring 21 is placed on top of the urine collection space 10. The urine outlet valve 16 of the urine catheter ring 21 and the electromagnetic urine collection valve 17 are in contact with each other and connected. The urine collection pump 6 introduces an appropriate amount of urine from the urine storage bag 9 into the sampling cylinder 7, and the urine detector 8 detects the urine test data.

[0072] Since the preparation of urokinase requires fresh urine, the freshest urine can be obtained by having the donor urinate directly into the urine collection container 1. When the urine is expelled, the first part of the urine will be contaminated with pollutants from the external genitalia. As the contaminants from the external genitalia are expelled along with the first part of the urine, the second part of the urine will not be contaminated and will be relatively clean. Therefore, it is necessary to detect whether the urine carries contaminants at the time of urination and separate the urine with contaminants from the clean urine in the second part.

[0073] When there are contaminants in the urine test data, the output urine test result is defined as the first test result. When the urine test result is the first test result, the urine detector 8 will test the urine in the urine collection bag 9 in the next urine collection space 10 again. The urine detector 8 will test the urine in the urine collection bag 9 in each urine collection space 10 in turn until there are no contaminants in the urine test data. Then the output urine test result is defined as the second test result. When the urine test result is the second test result, the urine detector 8 will stop testing the urine in the urine collection bag 9 in the subsequent urine collection space 10.

[0074] As urine continues to be discharged, the first part of the urine (contaminated urine) will be gradually drained, and after the first part of the urine is drained, the second part of the urine (uncontaminated urine) will be discharged. Therefore, by setting the urine capacity of each urine collection space 10 to be distributed in a structure that first decreases and then increases from bottom to top, the first part of the urine and the second part of the urine can be separated as accurately as possible.

[0075] When the urine test result is the second test result, it indicates that this part of the urine is the later part of the urine, and all subsequent urine is the later part of the urine. At this time, the urine detector 8 stops testing the urine in the urine collection bag 9 in the subsequent urine collection space 10.

[0076] Based on the urine test results, it can be determined whether the urine in the urine storage bag 9 of each urine collection space 10 is the front urine or the back urine. The front urine and the back urine are collected to obtain the first original liquid and the second original liquid, respectively. When collecting urine, the urine in the urine storage bag 9 of each urine collection space 10 is discharged sequentially from top to bottom along the urine storage tower 5.

[0077] The first original solution was acidified, centrifuged, and the precipitate was filtered out to obtain the first processed solution. The first processed solution was added to diatomaceous earth for adsorption. After elution with ammonia solution and a mixture of ammonia solution and sodium chloride solution, a turbid first sample solution was obtained. The first sample solution was filtered to obtain crude urokinase.

[0078] The second original solution was added to modified silica gel for adsorption. After elution with a mixture of sodium chloride solution and phosphoric acid solution and ammonium sulfate solution, a turbid second sample solution was obtained. The second sample solution was filtered and freeze-dried to obtain crude urokinase.

[0079] The above descriptions are merely embodiments of the present invention, and common knowledge regarding specific structures and characteristics is not elaborated upon here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. An apparatus for preparing urokinase, characterized in that: Includes urine collection device, sampling device, and detection device; The urine collection device is used to directly collect urine from the urine donor, the sampling device is used to obtain urine in each urine collection space (10) of the urine collection device, and the detection device is used to detect urine in each urine collection space (10). The urine collection device includes a urine collection container (1), a urine catheterization assembly, and a urine storage tower (5) connected in sequence. The urine collection container (1) is used to directly collect urine from the urine donor, and the urine catheterization assembly is used to transport the urine in the urine collection container (1) to the urine storage tower (5). The urine storage tower (5) is provided with multiple partition plates (18) from bottom to top. The partition plates (18) divide the urine storage tower (5) into multiple urine collection spaces (10). The urine collection spaces (10) are used to hold urine, and the urine capacity of each urine collection space (10) is distributed from bottom to top in a manner that first decreases and then increases.

2. The apparatus for preparing urokinase according to claim 1, characterized in that: Each urine collection space (10) is equipped with a urine storage component, which includes a urine guide ring (21) and a flexible urine storage bag (9). The urine guide ring (21) has a cavity inside. The top of the urine storage bag (9) is connected to the urine catheter ring (21), and the connection between the urine catheter ring (21) and the urine storage bag (9) is provided with an injection port for injecting urine into the urine storage bag (9). A first urine catheter port is opened on the side of the urine catheter ring (21), and a urine injection channel is formed between the first urine catheter port and the injection port. A first catheter valve (14) is installed on the first catheter port, and the first catheter valve (14) is a magnetic suction valve. The outer wall of the urine collection space (10) is provided with a first slide groove (12), and the first slide grooves (12) on the outer wall of adjacent urine collection spaces (10) are independent of each other. The first catheter valve (14) can slide in the first slide groove (12) of the urine collection space (10) in which it is located. The first urinary catheter valve (14) is connected to the urine outlet of the urinary catheter assembly, which can inject urine from the urine collection container (1) into the urine storage bag (9) through the first urinary catheter port and the urine injection port.

3. The apparatus for preparing urokinase according to claim 2, characterized in that: An annular groove (19) is formed on the partition plate (18). The bottom of the urine storage bag (9) is connected to the adjacent urine collection space (10) through the annular groove (19). The top of the urine guide ring (21) is provided with an annular engaging part (20) that can engage with the annular groove (19). When the urine storage bag (9) is filled with urine, the urine storage bag (9) gradually expands until it fills the urine collection space (10). The urine in the urine storage bag (9) will push the catheter ring (21) to move towards the upper partition plate (18) by the action of buoyancy. When the urine storage bag (9) is full of urine, the catheter ring (21) fits against the upper partition plate (18) and is placed on the top of the urine collection space (10). The annular locking part (20) of the catheter ring (21) engages with the annular through groove (19) to close the annular through groove (19) and separate the urine storage bag (9) from the adjacent urine collection space (10).

4. The apparatus for preparing urokinase according to claim 3, characterized in that: The catheterization assembly includes a catheterization pump (3), an inlet tube (2) is connected between the inlet end of the catheterization pump (3) and the urine collection container (1), an outlet tube (4) is connected between the outlet end of the catheterization pump (3) and the urine collection container (1), and the outlet of the outlet tube (4) is always higher than the outlet end of the catheterization pump (3), and the outlet tube (4) is a retractable elastic hose; The outlet of the urine tube (4) is connected to a second urine catheter valve (13), which is slidably connected to the outer wall of the urine storage tower (5). The outer wall of the urine storage tower (5) is provided with a second groove (11), and the second urine catheter valve (13) slides in the second groove (11). The second groove (11) is in contact with and connected to each of the first grooves (12). The second catheter valve (13) is a magnetic suction valve, and the second catheter valve (13) can be attracted to each of the first catheter valves (14) respectively. When the second catheter valve (13) and the first catheter valve (14) are attracted to each other, the second catheter valve (13) and the first catheter valve (14) are connected to each other. When the urine donor urinates directly into the urine collection container (1), the urine pump (3) guides the urine through the inlet tube (2) and the outlet tube (4) to the second urine valve (13). The second urine valve (13) and one of the first urine valves (14) attract each other and inject the urine into the urine storage bag (9) through the first urine valve (14), the first urine port and the urine injection port. When the urine collection bag (9) is filled with urine, the catheter ring (21) is placed at the top of the urine collection space (10). The first catheter valve (14) moves with the catheter ring (21) to the end of the first slide (12). The urine from the outlet tube (4) generates a lateral component impact force and a longitudinal component impact force on the second catheter valve (13). The longitudinal component impact force drives the second catheter valve (13) to move longitudinally along the second slide (11) and disengage from the currently engaged first catheter valve (14). At the same time, the first catheter valves (14) in the next first slide (12) engage with each other, so that the catheter pump (3) sequentially injects urine into the urine collection bags (9) of each urine collection space (10).

5. The apparatus for preparing urokinase according to claim 2, characterized in that: The catheter ring (21) has a second catheter port on its side, which is connected to the urine injection channel. The second catheter port is provided with a urine outlet valve (16). The side wall of the urine collection space (10) is provided with a third groove (15) that slides with the urine outlet valve (16). The second catheter port and the first catheter port are respectively located on both sides of the catheter ring (21), and the second catheter port is located above the first catheter port.

6. The apparatus for preparing urokinase according to claim 5, characterized in that: The sampling device includes a urine pump (6) and multiple sampling cylinders (7). An electromagnetic urine valve (17) is fixedly installed on the side wall of the urine collection space (10) and near the top of the urine collection space (10). One end of the urine pump (6) is connected to the electromagnetic urine valve (17) of each urine collection space (10), and the other end of the urine pump (6) is connected to multiple sampling cylinders (7). When the urine storage bag (9) is filled with urine, the urine catheter (21) is placed on top of the urine collection space (10). The urine outlet valve (16) of the urine catheter (21) and the electromagnetic urine collection valve (17) contact each other and conduct. The urine collection pump (6) introduces part of the urine in the urine storage bag (9) into the sampling cylinder (7).

7. The apparatus for preparing urokinase according to claim 5, characterized in that: The detection device includes a urine detector (8), the detection end of which is connected to each sampling tube (7) and is used to detect the urine in the sampling tube (7) and output urine detection data.

8. A preparation process using the urokinase preparation apparatus according to any one of claims 1-7, characterized in that: Includes the following steps: S1: The urine donor urinates directly into the urine collection device; S2: The urine collection device stores urine in each urine collection space (10) of the urine collection device according to the time of urination, and the sampling device obtains the urine in each urine collection space (10); S3: The detection device detects the urine in each urine collection space (10) and generates a first detection result and a second detection result. The urine collection device discharges the urine in the urine collection space (10) with the first detection result to obtain the first original liquid, and discharges the urine in the urine collection space (10) with the second detection result to obtain the second original liquid. S3-1: After acidifying the first stock solution, centrifuge and filter out the precipitate to obtain the first treatment solution. Add diatomaceous earth to the first treatment solution for adsorption. Elute with elution buffer I and elution buffer II in sequence to obtain a turbid first sample solution. Filter the first sample solution to obtain crude urokinase. S3-2: The second original solution was added to modified silica gel for adsorption. After elution with elution buffer III and elution buffer IV in sequence, a turbid second sample solution was obtained. The second sample solution was filtered and freeze-dried to obtain crude urokinase.

9. The preparation process of urokinase according to claim 8, characterized in that: The eluent I is an ammonia solution, and the eluent II is a mixture of an ammonia solution and a sodium chloride solution.

10. The preparation process of urokinase according to claim 9, characterized in that: The eluent III is a mixture of sodium chloride solution and phosphoric acid solution, and the eluent IV is an ammonium sulfate solution.

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