Preparation method of self-oxygen-supplying acoustic power nano-preparation and application thereof in preparation of cancer treatment drugs

By preparing self-oxygenated sonodynamic nanoparticles, the problem of low efficiency of SDT in hypoxic tumor microenvironment was solved, the therapeutic effect of PD-1/PD-L1 was enhanced, the immune response was activated, and effective killing of tumor cells and immune memory were achieved.

CN116019912BActive Publication Date: 2026-03-03THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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Patent Information

Application Number
CN202310056686.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2022-10-27
Filing Date
2023-01-16
Publication Date
2026-03-03
Estimated Expiration
2043-01-16

AI Technical Summary

Technical Problem

Existing sonodynamic therapy (SDT) has low efficacy in hypoxic tumor microenvironments, and PD-1/PD-L1 immune checkpoint inhibitors have limited therapeutic effects, failing to effectively activate tumor antigen-specific T cell infiltration and the immunosuppressive microenvironment.

Method used

A self-oxygenating sonic dynamic nanoparticle formulation was prepared by encapsulating a sonic sensitizer IR-780, an immune adjuvant Vadimezan (DMXAA), and fluorocarbon droplets PFC through a thin-film hydration method to form nanolipid droplets with a particle size of 100 nm-2 μm, which can release oxygen and activate an immune response under ultrasonic irradiation.

Benefits of technology

It significantly improves the efficiency of sonodynamic therapy, enhances the effect of PD-1/PD-L1 therapy, promotes tumor cell apoptosis and immunogenic necrosis, activates CTL infiltration, improves the tumor microenvironment, inhibits in situ and distant tumor growth, and reduces toxic side effects.

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Abstract

This invention discloses a method for preparing a self-oxygenated acoustic-dynamic nanoparticle and its application in the preparation of cancer treatment drugs. The self-oxygenated acoustic-dynamic nanoparticle includes a carrier, on which nanolipid droplets containing a sound-sensitizing agent, the immune adjuvant Vadimezan, and fluorocarbon droplets PFC are coated. The preparation method includes the following steps: (1) preparing carrier raw materials, and coating the sound-sensitizing agent, the immune adjuvant Vadimezan, and the fluorocarbon droplets PFC into a mixed solution by thin-film hydration; (2) dispersing the mixed solution under water bath ultrasound and probe ultrasound conditions, and dialyzing after the procedure; (3) filling the product obtained in step (2) with oxygen and storing it in a refrigerator at 4°C. The self-oxygenated acoustic-dynamic nanoparticle has good biocompatibility. It generates singlet oxygen that can kill tumor cells through ultrasound irradiation, and has spatiotemporal controllability, which can effectively improve the therapeutic effect and reduce toxic side effects. It can not only effectively inhibit the progression of in situ tumors, but also effectively inhibit the growth of distant tumors.
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Citation Information

Patent Citations

  • Novel composite nano preparation based on sonodynamic / immune combined therapy and preparation method and application thereof

    CN112933227A