提高谷氨酸棒杆菌生产色氨酸的方法
By introducing exogenous phosphotransketase and phosphoacetyltransferase pathways into Corynebacterium glutamicum and combining them with the inactivated pyruvate kinase gene pyk, recombinant Corynebacterium glutamicum was constructed, which solved the problem of low tryptophan production and achieved a significant increase in tryptophan production.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- TSINGHUA UNIVERSITY
- Filing Date
- 2022-11-10
- Publication Date
- 2026-07-17
AI Technical Summary
The low yield of tryptophan in existing technologies leads to high production costs. Improving the content of erythrose-4-phosphate and phosphoenolpyruvate to increase tryptophan yield without affecting cell growth is an important challenge.
By introducing exogenous phosphotransketase and phosphoacetyltransferase pathways into Corynebacterium glutamicum, and increasing the synthesis of erythrose-4-phosphate and phosphoenolpyruvate in cells through the inactivation of the pyruvate kinase gene pyk, recombinant Corynebacterium glutamicum was constructed to increase tryptophan production.
It significantly increased tryptophan production, effectively increasing the content of erythrose-4-phosphate and phosphoenolpyruvate without affecting cell growth, and reducing production costs.