一种螨虫过敏原检测试纸及其制备方法

By introducing a protein filter layer and a urea filter layer into the mite allergen test strip, combined with a diazotization chemical reaction layer, the problem of environmental interference in existing detection methods is solved, achieving high sensitivity and high accuracy in mite allergen detection.

CN116046769BActive Publication Date: 2026-07-17HANGZHOU MIAOQI TECH INNOVATION CO LTD

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HANGZHOU MIAOQI TECH INNOVATION CO LTD
Filing Date
2023-02-03
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing methods for detecting dust mite allergens lack sufficient sensitivity and accuracy, and cannot effectively eliminate interference from environmental proteins and urea, leading to inaccurate test results.

Method used

Design a mite allergen test strip comprising a protein filter layer, a urea filter layer, and a diazotization chemical reaction layer. It filters out impurities such as proteins and urea through a protein precipitant and urease powder, and utilizes a colorimetric reaction between aromatic diazo compounds or azo compounds and guanine to achieve rapid detection.

Benefits of technology

It improves the sensitivity and accuracy of detection, enabling rapid and accurate determination of mite allergen content. The results are clear and easy to visually identify, making it suitable for convenient and efficient environmental monitoring.

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Abstract

本发明涉及生化分析检测技术领域,具体涉及一种螨虫过敏原检测试纸及其制备方法。该试纸包括蛋白质过滤层、尿素过滤层和重氮化化学反应层。其中,蛋白质过滤层起到截留蛋白质的作用,消除样品中杂蛋白的干扰;尿素过滤层起到分解尿素氮的作用,消除尿素对检测结果的干扰;过滤后样品中鸟嘌呤与重氮化化学反应层中芳香族重氮化合物或偶氮化合物发生颜色反应,颜色深浅与鸟嘌呤含量成正比,从而实现环境中螨虫过敏原的检测。本发明通过蛋白质过滤层和尿素过滤层的过滤,可提高螨虫过敏原的检测灵敏度和准确度。
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