An antioxidant active polypeptide and a preparation method and application thereof

By preparing earthworm polypeptides F1-2 and F1-3, the problem of insufficient efficacy of existing antioxidant polypeptides has been solved, achieving highly efficient free radical scavenging and antioxidant effects. These polypeptides can be applied to pharmaceuticals, food additives, and skin care products, avoiding the side effects of artificial synthesis.

CN116063383BActive Publication Date: 2026-07-24GUANGZHOU NURSING HOME (GUANGZHOU GERIATRIC HOSPITAL)
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGZHOU NURSING HOME (GUANGZHOU GERIATRIC HOSPITAL)
Filing Date
2023-01-30
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing antioxidant peptides are not sufficiently effective in antioxidation and may have side effects due to their artificial synthesis.

Method used

Two earthworm polypeptides, F1-2 and F1-3, were prepared using artificial synthesis methods. The amino acids were coupled one by one and purified using the Fmoc polypeptide solid-phase synthesis method. Specific small molecule polypeptide fragments were extracted from earthworms by combining earthworm enzymatic hydrolysis, ultrafiltration, and gel chromatography purification techniques.

Benefits of technology

Earthworm polypeptides F1-2 and F1-3 exhibit significant antioxidant effects, effectively scavenging DPPH free radicals and alleviating premature aging caused by oxidative stress. They can be applied in pharmaceuticals, food additives, and skincare products, and are low-cost, safe, and without side effects.

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Abstract

The application discloses an antioxidative active polypeptide and a preparation method thereof, the polypeptide is F1-2 or F1-3, the amino acid sequence of F1-2 is EGKDEFRL, and the amino acid sequence of F1-3 is EIRIKHADRK. The application further discloses an earthworm polypeptide and a preparation method thereof, the earthworm polypeptide comprises the peptide segments F1-2 and F1-3. The application further discloses application of the antioxidative active polypeptide or the earthworm polypeptide in preparation of an antioxidant drug, a food additive, health products or skin care products. Two kinds of amino acid sequence determined bioactive peptide segments are identified from the earthworm polypeptide, the bioactive peptide segments are verified to have a significant antioxidation effect, a DPPH free radical clearance rate is very high, animal experiments show that the bioactive peptide segments can relieve premature aging caused by oxidative stress, and the bioactive peptide segments can be used in preparation of the antioxidant drug, the health products, the food additive or the skin care products, and have a good application prospect.
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