Closed microalgae culture device light exchange automatic stirring device

CN116064209BActive Publication Date: 2026-09-22RIZHAO VOCATIONAL & TECHNICAL UNIVERSITY
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Patent Information

Application Number
CN202211598160.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-12-14
Publication Date
2026-09-22
Estimated Expiration
2042-12-14

AI Technical Summary

Technical Problem

[0005]本发明要实现的目标是:解决密闭式微藻培养现有技术中,仅通过气泡搅动培养液,搅动能力有限,不能实现培养液的充分搅动,不利于气体在培养液中充分溶解,也不利于微藻在培养液中游动分布的技术问题

Benefits of technology

[0016]本发明的积极效果是:经过反射,光能够更有效的传递到培养器的边缘,同时搅动杆内接通气体,在搅动的同时,实现对培养器内供气,而供气同时带动反光镜进行转动,实现局部搅动的同时,改变了光的折射路径,使得光可以传递到不同的区域,同时为微藻提供没有光的暗时间,达到间歇光照效应,实现光能的充分利用,能实现培养液的充分搅动,有利于气体在培养液中充分溶解,更有利于微藻在培养液中游动分布,避免微藻聚堆,导致培养液局部养分消耗不均的问题。

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Abstract

The application discloses a closed microalgae culture device light exchange automatic stirring device, which comprises a central pipe and a plurality of stirring rods fixed on the outer side of the central pipe; the central pipe drives the stirring rods to rotate and emits light required by microalgae; each stirring rod is provided with a plurality of reflectors, the frame of the reflector is solid on one side and is provided with a first cavity on the other side; the first cavity is connected with a plurality of air nozzles fixed on the same side of the frame; the stirring rod is provided with a second cavity, and the first cavity is communicated with the second cavity. The beneficial effects are as follows: after reflection, the light can be more effectively transmitted to the edge of the culture device; the gas is connected in the stirring rod; the gas supply in the culture device is realized while the stirring rod is stirred; the reflector is driven to rotate while the gas supply is realized; the light refraction path is changed while the local stirring is realized; the light can be transmitted to different areas; the dark time for the microalgae is provided; the intermittent light effect is achieved; and the full use of light energy is realized.
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Description

Technical Field

[0001] This invention belongs to the technical field of closed microalgae culture devices, and particularly relates to an automatic light exchange stirring device for closed microalgae culture devices. Background Technology

[0002] Intermittent light effect refers to the process by which Chlorella proteoglycans fully and effectively utilize the light energy of incident light during photosynthesis. The dark time must be at least 10 seconds of the flash time, and the cell intermittent exposure time is 30 seconds to achieve the intermittent light effect.

[0003] In the current closed-loop microalgae cultivation process, artificial light sources are mainly set up in the microalgae culture vessel, and gas is introduced. The gas rises in the culture medium in the form of bubbles, so as to replenish the culture medium with gas and agitate it.

[0004] However, simply agitating the culture medium with bubbles has limited agitation capacity and cannot achieve sufficient agitation. This is not conducive to the full dissolution of gases in the culture medium, nor is it conducive to the movement and distribution of microalgae in the culture medium. Summary of the Invention

[0005] The objective of this invention is to solve the technical problems in existing closed-system microalgae cultivation technologies, where the agitation of the culture medium is limited by only using air bubbles, which fails to achieve sufficient agitation of the culture medium, hinders the full dissolution of gases in the culture medium, and is also detrimental to the movement and distribution of microalgae in the culture medium.

[0006] To achieve the above objectives, the present invention provides an automatic agitation device for light exchange in a closed microalgae culture vessel.

[0007] The specific technical solution adopted in this invention is as follows: An automatic light exchange stirring device for a closed microalgae culture device includes a central tube and several stirring rods evenly distributed around the outer circumference of the central tube; the central tube drives the stirring rods to rotate and emits the light required by the microalgae; each stirring rod is provided with several reflectors, one side of the frame of the reflector is solid, and the other side is provided with a first cavity, the first cavity is connected to several air nozzles fixed on the same side of the frame, and the stirring rod is provided with a second cavity, the first cavity and the second cavity are connected; The second cavity is connected to an external gas source. Gas enters the first cavity from the second cavity and then exits from the gas nozzle, causing the reflector to rotate on the stirring rod. The light emitted from the central tube is reflected by the reflector to the edge of the incubator.

[0008] Preferably, the central tube has an opening at one end located outside the culture vessel, and an external reflector is provided at the opening to reflect sunlight or artificial light into the interior of the central tube, providing light for the culture vessel.

[0009] Preferably, the central tube is equipped with an artificial light source to provide light for the culture vessel.

[0010] Preferably, a first gear is fitted on one end of the central tube located outside the incubator, and the first gear meshes with a drive motor.

[0011] Preferably, the central tube is provided with a spoke-shaped support ring, and the stirring rods are evenly distributed around the circumference of the spoke-shaped support ring.

[0012] Preferably, the mirror surface of the reflector is a spherical mirror surface.

[0013] Preferably, a pivot is provided on the symmetrical center line of the frame of the reflector, the pivot has a third cavity, the third cavity is connected to the first cavity and the second cavity, the stirring rod has a hollow U-shaped bracket, and the frame is connected to the U-shaped bracket through the pivot.

[0014] Preferably, the hollow U-shaped support has a through hole in its tube wall, a bearing is installed in the through hole, the inner ring of the bearing is engaged with the rotating shaft, the outer ring of the bearing is engaged with the through hole, and a sealing ring is provided on the outer side of the bearing.

[0015] Preferably, the U-shaped supports on adjacent stirring rods are evenly distributed around the central tube as the axis to form a reflective stirring layer, and the stirring device is provided with several layers of the reflective stirring layer.

[0016] The positive effects of this invention are as follows: After reflection, light can be more effectively transmitted to the edge of the culture vessel. At the same time, gas is supplied to the culture vessel while stirring. The gas supply drives the reflector to rotate, achieving local stirring and changing the refraction path of light, allowing light to be transmitted to different areas. It also provides dark time without light for microalgae, achieving an intermittent lighting effect and making full use of light energy. It can achieve sufficient stirring of the culture medium, which is conducive to the full dissolution of gas in the culture medium and more conducive to the movement and distribution of microalgae in the culture medium, avoiding the problem of uneven nutrient consumption in the culture medium caused by microalgae aggregation. Attached Figure Description

[0017] Figure 1 This is a front view of an automatic agitation device for light exchange in a closed microalgae culture vessel according to the present invention; Figure 2 yes Figure 1 Sectional view along line AA; Figure 3 yes Figure 1 The image shows a partial view of an automatic light exchange stirring device for a closed microalgae culture vessel according to the present invention. Figure 4 yes Figure 3 Enlarged view of point E shown; Figure 5 yes Figure 3 The schematic diagram of the reflector shown Legend: 1—First gear, 2—First spoke, 3—Central tube, 4—Reflector, 401—Right side frame, 402—Left side frame, 403—Gas nozzle, 5—Stirring rod, 6—Second spoke, 7—Gas concentration detector, 8—Inlet pipe, 9—Support spoke, 10—First connector, 11—U-shaped bracket, 12—Second connector, 13—Speaker cavity, 14—Connecting pipe, 15—Shaft. Detailed Implementation

[0018] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments: In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0019] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0020] The specific implementation of the present invention will be described in detail below with reference to specific embodiments.

[0021] Example 1: like Figure 1 and Figure 2 The diagram shows the structure of an automatic light-exchange stirring device for a closed microalgae culture vessel according to an embodiment of the present invention. The stirring device includes a central tube 3 and several stirring rods 5 evenly distributed around the outer circumference of the central tube. The central tube 3 drives the stirring rods 5 to rotate and emits the light required by the microalgae. Each stirring rod 5 is equipped with several reflectors 4. One side of the frame of each reflector 4 is solid, and the other side has a first cavity. Figure 5 As shown, the first cavity is connected to several air nozzles 403 fixed on the same side of the frame, specifically, as follows: Figure 4As shown, the frame of the reflector is divided into a left frame 402 and a right frame 401. The right frame 401 is provided with a first cavity, which is connected to several air nozzles 403. The stirring rod 5 is provided with a second cavity, which is connected to the first cavity. The second cavity is connected to an external air source. Gas enters the first cavity from the second cavity and then exits from the air nozzles 403, causing the reflector 4 to rotate on the stirring rod 5. The light emitted by the central tube 3 is reflected by the reflector 4 to the edge of the incubator.

[0022] In this embodiment of the invention, the light emitted from the central tube is reflected by the reflector, allowing it to be more effectively transmitted to the edge of the culture vessel. Simultaneously, gas is supplied to the culture vessel during agitation, which in turn drives the reflector to rotate. This localized agitation alters the light refraction path, enabling the light to reach different areas. It also provides the microalgae with a dark period, achieving an intermittent lighting effect and maximizing the utilization of light energy. This process ensures thorough agitation of the culture medium, facilitating the complete dissolution of gas and promoting the free movement and distribution of microalgae. It also prevents microalgae from accumulating and causing uneven nutrient consumption in the culture medium.

[0023] In one embodiment of the present invention, the central tube 3 has an opening at one end located outside the culture vessel, and an external reflector is provided at the opening. The external reflector reflects sunlight or artificial light into the interior of the central tube to provide light for the culture vessel.

[0024] In another embodiment of the present invention, an artificial light source is provided in the central tube 3 to provide light to the culture vessel. By energizing the artificial light source, the artificial light source emits light to provide light energy to the microalgae in the culture vessel for photosynthesis.

[0025] In particular, artificial light with a wavelength of 390-410nm can activate chloroplast movement; while red light with a wavelength of 600-700nm can enhance chloroplast photosynthesis. Therefore, using artificial light sources, such as artificial light, can effectively control the intensity of photosynthesis and is more conducive to controlling the growth rate of microalgae in microalgae cultivation.

[0026] In another embodiment of the invention, such as Figure 1 and Figure 2As shown, a first gear 1 is fitted onto one end of the central tube 3 located outside the culture vessel. The first gear 1 meshes with a drive motor, which drives the first gear 1 to rotate, thus rotating the central tube 3. The central tube 3 has a spoke-shaped support ring, and stirring rods 5 are evenly distributed around the circumference of the spoke-shaped support ring. Specifically, a support plate 9 is provided at the top of the central tube 3, and the support plate 9 is connected to the wall of the culture vessel. Rollers are provided on the edge of the support plate 9. When the drive motor drives the first gear 1 to rotate, the first gear 1 drives the support plate 9 to rotate on the circumferential wall of the culture vessel, which is used to fix the entire vessel. The mirror 4 is positioned and maintains a circular motion. Below the supporting spoke 9 is a first spoke 2, below the first spoke 2 are a second spoke 6 and a bottom spoke. At the bottom of the bottom spoke is a gas concentration detector 7. The stirring rod 5 connects the first spoke 2, the second spoke 6 and the bottom spoke in a circular series to form a cage structure. A rotating shaft 15 is provided on the symmetrical center line of the frame of the reflector 4. The rotating shaft 15 has a third cavity, which is connected to the first cavity and the second cavity. The stirring rod 5 has a hollow U-shaped bracket 11. The frame is connected to the U-shaped bracket 11 through the rotating shaft 15.

[0027] Preferably, the hollow U-shaped support 11 has a through hole in its tube wall, and a bearing is installed in the through hole. The inner ring of the bearing is engaged with the rotating shaft 15, and the outer ring of the bearing is engaged with the through hole. A sealing ring is provided on the outer side of the bearing. In this way, the frame is connected to the U-shaped support 11, and the first cavity in the right frame 401 is connected to the second cavity through the third cavity in the rotating shaft 15, so as to facilitate the entry of external gas.

[0028] When the gas concentration detector 7 detects that the gas concentration in the culture medium does not meet the requirements, the outside air supplies gas to the first cavity through the second cavity and the third cavity. At the same time, the pneumatic drive motor drives the first gear 1 to rotate, which in turn drives the stirring rod 5 to rotate. The pressurized gas is ejected from the first cavity through the gas nozzle 403. Since the frame is divided into a left frame 402 and a right frame 401, with the right frame 401 having the first cavity and the left frame being solid, the gas ejected from the right frame creates a torque that causes the frame to rotate around the rotating shaft 15. This torque causes the reflector 4 to rotate around the rotating shaft 15. The light emitted from the central tube is reflected by the reflector 4, allowing the light to be transmitted more effectively to the edge of the culture vessel. At the same time, the stirring rod is connected to gas, and gas is supplied to the culture vessel while stirring. The gas supply also drives the reflector to rotate, achieving local stirring while changing the light refraction path, allowing the light to be transmitted to different areas. This also provides the microalgae with a dark period without light, achieving an intermittent lighting effect and making full use of light energy.

[0029] Example 2: Based on Embodiment 1, a connector is provided between the first gear 1 and the supporting spoke. The connector is provided with an air inlet pipe 8, which is connected to a connecting pipe 14. The connecting pipe 14 is connected to the inside of the connector through a bearing. The top of the connector is connected to the air inlet pipe 8 through a small cavity of the connector. The supporting spoke at the bottom is provided with a spoke cavity 13, which is connected to the connecting pipe 14. The spoke cavity 13 is connected to the second cavity of the stirring rod 5 through a second connector 12. The second cavity of the stirring rod 5 is connected to the U-shaped bracket 11 through a first connector 10. The support arm of the U-shaped bracket 11 is hollow. The second cavity is connected to the first cavity of the right frame 401 through the hollow part of the support arm and through a third cavity, forming a gas passage. This design can ensure that the air inlet pipe is stationary while the stirring rod can rotate at any angle, which facilitates the fixed-point air supply to the rotating parts.

[0030] Example 3: The U-shaped supports 11 on the adjacent stirring rods 5 are evenly distributed around the central tube as the axis to form a reflective stirring layer. The stirring device is provided with several layers of the reflective stirring layer.

[0031] Multi-layered reflectors enable multi-level agitation and reflection. Gas supply simultaneously rotates the reflectors, achieving localized agitation while altering the light refraction path, allowing light to reach different areas. This provides dark periods for the microalgae, achieving an intermittent lighting effect and maximizing light energy utilization. It also ensures thorough agitation of the culture medium, facilitating gas dissolution and promoting the free movement and distribution of microalgae, preventing algal aggregation and uneven nutrient consumption. The foregoing has broadly outlined some aspects and features of the various embodiments and should be interpreted as merely illustrative of potential applications. Other beneficial results can be obtained by applying the disclosed information in different ways or by combining various aspects of the disclosed embodiments. Further aspects and a more comprehensive understanding can be obtained based on the specific description of exemplary embodiments with reference to the accompanying drawings, within the scope defined by the claims.

[0032] The above embodiments provide a detailed description of the present invention. Of course, the above description is not intended to limit the present invention, nor is the present invention limited to the examples described above. Any changes, modifications, additions, reductions, or substitutions made by those skilled in the art within the scope of the present invention are also within the protection scope of the present invention.

Claims

1. An automatic light exchange stirring device for a closed microalgae culture vessel, characterized in that, The stirring device includes a central tube and several stirring rods evenly distributed around the outer circumference of the central tube. The top of the central tube is provided with a supporting plate, a first plate is provided below the supporting plate, a second plate and a bottom plate are provided below the first plate, and the stirring rods connect the circumferences of the first plate, the second plate and the bottom plate in series to form a cage-like structure. The central tube drives the stirring rods to rotate and emits the light required by the microalgae. Each stirring rod is provided with several reflectors. One side of the frame of the reflector is solid, and the other side has a first cavity. The first cavity is connected to several air nozzles fixed on the same side of the frame. The stirring rod has a second cavity, and the first cavity communicates with the second cavity. The second cavity is connected to an external gas source. Gas enters the first cavity from the second cavity and then exits from the gas nozzle, causing the reflector to rotate on the stirring rod. The light emitted from the central tube is reflected by the reflector to the edge of the incubator. The central tube is provided with a spoke-shaped support ring, and the stirring rods are evenly distributed around the circumference of the spoke-shaped support ring; A pivot is provided on the symmetrical center line of the reflector frame, and the pivot has a third cavity. The third cavity is connected to the first cavity and the second cavity. The stirring rod has a hollow U-shaped bracket, and the frame is connected to the U-shaped bracket through the pivot.

2. The automatic light exchange stirring device for a closed microalgae culture vessel according to claim 1, characterized in that, The central tube has an opening at one end located outside the culture vessel, and an external reflector is provided at the opening to reflect sunlight or artificial light into the interior of the central tube, providing light for the culture vessel.

3. The automatic light exchange stirring device for a closed microalgae culture vessel according to claim 1, characterized in that, The central tube contains an artificial light source to provide light for the culture vessel.

4. The automatic light exchange stirring device for a closed microalgae culture vessel according to any one of claims 1 to 3, characterized in that, A first gear is fitted onto one end of the central tube located outside the incubator, and the first gear meshes with a drive motor.

5. The automatic light exchange stirring device for a closed microalgae culture vessel according to claim 4, characterized in that, The mirror surface is a spherical mirror surface.

6. The automatic light exchange stirring device for a closed microalgae culture vessel according to claim 5, characterized in that, The hollow U-shaped support has a through hole in its tube wall, and a bearing is installed inside the through hole. The inner ring of the bearing is fitted with the rotating shaft, the outer ring of the bearing is fitted with the through hole, and a sealing ring is provided on the outer side of the bearing.

Citation Information

Patent Citations

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