定点同步敲入和敲除基因的抗凋亡细胞改造方法及其应用

By disrupting the FUT8 gene and introducing the Bcl-2 gene into CHO cells, and using CRISPR/Cas9 technology to achieve site-specific synchronous knock-in and knockout of CHO cells, the problem of decreased yield and quality caused by CHO cell apoptosis was solved, and the anti-apoptotic performance and recombinant protein production efficiency were improved.

CN116064398BActive Publication Date: 2026-07-17SHANGHAI BIOENGINE SCI-TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI BIOENGINE SCI-TECH CO LTD
Filing Date
2022-07-04
Publication Date
2026-07-17

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Abstract

本发明提供了定点同步敲入和敲除基因的抗凋亡细胞改造方法及其应用。具体地，本发明提供了一种遗传工程化的抗凋亡CHO细胞(中国仓鼠卵巢细胞)，所述抗凋亡CHO细胞中内源的FUT8(岩藻糖转移酶8)基因被破坏；并且，在所述细胞的被破坏的FUT8基因的位点，导入了抗凋亡的外源基因的表达盒，所述抗凋亡的外源基因为Bcl‑2(B细胞淋巴瘤2)基因。本发明的抗凋亡细胞具有优异显著的抗凋亡性能，并具备生产无岩藻糖抗体的能力。本发明的基因工程改造方法的操作简便，时间成本低。
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