A timing temperature control magnetic bead drying device

Abstract

The application discloses a kind of timing temperature control magnetic magnetic bead drying device, including box, magnetic metal block, clamping assembly and drying assembly, box is installed on the bottom plate, the shock-absorbing assembly is installed on the bottom plate, the magnetic metal block is fixedly installed on shock-absorbing assembly, the clamping assembly is installed on the magnetic metal block, the bottom of the box is equipped with drying assembly on both sides, the magnetic magnetic bead in EP tube is adsorbed by the magnetic metal block, cooperate drying assembly from bottom to top blow, the experiment of extracting nucleic acid to different magnetic bead quantity, corresponding operation standard can be customized by changing heating temperature and blowing time, meet the demand of different geographical different experiment different texture magnetic bead to different drying degree, avoid because residual liquid causes experimental specimen internal reference not to come out, curve is inhibited, reduce and exempt from the reagent waste caused by experiment failure.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid extraction technology, specifically to a timed temperature-controlled magnetic bead drying device. Background Technology

[0002] Magnetic bead elution is a commonly used method for extracting nucleic acids in molecular biology, offering advantages such as speed and convenience. The final step involves eluting nucleic acids from the magnetic beads. Prior to this, the beads are used to adsorb the nucleic acids, followed by thorough drying to ensure complete evaporation of solvents such as ethanol or isopropanol from the washing solution. This prevents the presence of non-reaction impurities in the eluted solution, ensuring the purity and quality of the nucleic acids and preventing any impact on subsequent amplification experiments.

[0003] Traditional magnetic bead extraction of nucleic acids involves either air drying with the cap open after washing the magnetic beads, or placing the opened nucleic acid extraction tubes at the air outlet of a biosafety cabinet for air drying. The former is time-consuming and prone to leaving organic solvent residues, while the latter can easily cause aerosol contamination inside the biosafety cabinet. Summary of the Invention

[0004] The purpose of this invention is to provide a timed and temperature-controlled magnetic bead drying device to solve the problems mentioned in the background art.

[0005] In the description of this invention, it should be noted that the terms "inner," "outer," "upper," "lower," etc., indicating orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of the invention is in use. They are merely for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed or operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, the terms "first," "second," etc., are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0006] This invention discloses a timed temperature-controlled magnetic bead drying device, comprising a housing, a magnetic metal block, a clamping assembly, and a drying assembly. The housing is mounted on a base plate, and a shock-absorbing assembly is mounted on the base plate. A magnetic metal block is fixedly mounted on the shock-absorbing assembly, and a clamping assembly is mounted on the magnetic metal block. Drying assemblies are mounted on both sides of the bottom of the housing, and drying assemblies are mounted on both sides of the top of the housing. The drying assemblies are connected to the drying assemblies. The shock-absorbing assembly includes a trapezoidal base, a mounting plate, and a connecting rod. The trapezoidal base is mounted at the center of the bottom of the housing. The trapezoidal base has a trapezoidal cross-section and several sets of guide grooves symmetrically arranged on it. Sliding blocks are slidably fitted in the guide grooves. The sliding blocks are connected to the mounting plate via the connecting rod. A limit ring is mounted on the top of the guide groove, and the connecting rod is slidably fitted with the limit ring. A first spring is provided between the sliding block and the limit ring, and the first spring is rotatably fitted with the connecting rod. A second spring is provided between the sliding block and the bottom of the guide groove. The two sides of the mounting plate are connected to the top of the trapezoidal base via a flexible windproof cloth.

[0007] Preferably, a door is rotatably installed on the top of the enclosure.

[0008] Preferably, the clamping assembly includes a fixing plate, rings, and EP tubes. The fixing plate is installed on the top of the magnetic metal block, and several sets of rings are arranged on both sides of the fixing plate. EP tubes are installed inside the rings, with the openings of the EP tubes facing upwards.

[0009] Preferably, the cross-section of the magnetic metal block is trapezoidal, and the ring is inclined downwards.

[0010] Preferably, the drying assembly includes a gas collection box, perforated plates, and absorbent filling material. The gas collection box is installed on both sides of the top of the chamber. A through groove is provided on the top side of the gas collection box away from the chamber. A grid plate is installed in the through groove. A temperature sensor is installed on the grid plate. The temperature sensor is used to collect the temperature of the air passing through the grid plate and send the temperature signal to the control module. The control module is installed on the chamber. Several sets of perforated plates are installed in the gas collection box. Absorbent filling material is filled between the perforated plates. Through holes are provided at the bottom of the gas collection box.

[0011] Preferably, a first electric heating module is provided between adjacent groups of water-absorbing filling materials, a humidity sensor is installed at the bottom of the air collection box, the humidity sensor is used to collect the air humidity in the air collection box and send the humidity signal to the control module, a solenoid valve is installed on the through hole, and an exhaust pipe is connected to the side of the air collection box near the box body.

[0012] Preferably, the drying assembly includes a support plate, a drive motor, and a partition. The top of the partition is connected to the air collection box, and the bottom of the partition is connected to the housing. An air outlet groove is provided on the partition, and several sets of baffles are rotatably installed in the air outlet groove. Support plates are installed on both sides of the bottom of the housing, and several sets of through holes are provided on the support plates. A drive motor is installed on the support plate, and a fan blade is installed at the output end of the drive motor. A second electric heating module is installed between the drive motor and the partition, and a mesh cover is installed on the drive motor.

[0013] Preferably, the control module includes a signal processing module, a temperature signal acquisition module, a humidity signal acquisition module, and an adjustment module. The temperature signal acquisition module receives temperature signals from a temperature sensor and sends them to the signal processing module. The humidity signal acquisition module receives humidity signals from a humidity sensor and sends them to the signal processing module. The signal processing module processes the data sent by the temperature and humidity signal acquisition modules and compares them with preset data within the signal processing module. After comparing the data, the signal processing module sends a feedback signal to the adjustment module. The adjustment module is connected to the terminals of the first heating module, the solenoid valve, and the second heating module.

[0014] Compared with existing technologies, this method uses a magnetic metal block to adsorb magnetic beads in an EP tube, combined with a bottom-up airflow from a drying component. For nucleic acid extraction experiments with different amounts of magnetic beads, the corresponding operating standards can be customized by changing the heating temperature and airflow time. This meets the needs of different regions, experiments, and types of magnetic beads for different degrees of drying, avoiding the failure of internal controls in experimental samples due to residual liquid, inhibiting curves, and reducing reagent waste, manpower waste, order delays, and increased patient suffering caused by experimental failures. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of a timed temperature-controlled magnetic bead drying device according to the present invention.

[0016] Figure 2 This is a schematic diagram of region A in a timed temperature-controlled magnetic bead drying device of the present invention.

[0017] Figure 3 This is a schematic diagram of region B in a timed temperature-controlled magnetic bead drying device of the present invention.

[0018] Figure 4 This is a schematic diagram of the control module in a timed temperature-controlled magnetic bead drying device of the present invention.

[0019] Figure label: 1-Base plate, 2-Box body, 3-Shock absorption assembly, 31-Trapezoidal base, 32-Mounting plate, 33-Guide groove, 34-Sliding block, 35-Limiting ring, 36-Connecting rod, 37-First spring, 38-Second spring, 39-Flexible windproof cloth, 4-Magnetic metal block, 5-Clamping assembly, 51-Fixing plate, 52-Ring, 53-EP tube, 6-Drying assembly, 61-Gas collection box, 62-Grid plate, 63-Perforated plate, 64-Water-absorbing filler Materials: 65-First heating module, 66-Solenoid valve, 67-Exhaust pipe, 68-Humidity sensor, 69-Through hole, 7-Drying assembly, 71-Support plate, 72-Drive motor, 73-Mesh cover, 74-Second heating module, 75-Baffle, 76-Break plate, 8-Control module, 81-Signal processing module, 82-Temperature signal acquisition module, 83-Humidity signal acquisition module, 84-Adjustment module, 9-Door, 10-Temperature sensor. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention. Example

[0021] like Figure 1As shown, a timed and temperature-controlled magnetic bead drying device includes a housing 2, a magnetic metal block 4, a clamping assembly 5, and a drying assembly 7. The housing 2 is mounted on a base plate 1, on which a shock-absorbing assembly 3 is installed. The magnetic metal block 4 is fixedly mounted on the shock-absorbing assembly 3, which provides shock absorption to prevent the magnetic metal block 4 from shaking significantly during use. The clamping assembly 5 is installed on the magnetic metal block 4 and is used to hold the magnetic beads. Under the action of the magnetic metal block 4, the magnetic beads in the clamping assembly 5 tend to move closer to the magnetic metal block 4, ensuring that the magnetic beads will not be blown out during the drying process. The drying assembly 7 is installed on both sides of the bottom of the housing 2. The drying assembly 7 can spray heated gas into the center of the housing 2. Despite the interference of the shape of the metal block 4, the high-temperature gas ejected from the drying components 7 on both sides surges from the bottom of the clamping component 5 to the top of the clamping component 5. This heats the gas inside the clamping component 5 and drives it to evaporate upwards, preventing the magnetic beads inside the clamping component 5 from generating aerosols and causing potential pollution due to direct airflow. Drying components 6 are installed on both sides of the top of the box 2. The drying components 6 are connected to the drying components 7. The gas rising to the top of the box 2 can be introduced into the drying components 6 through the exhaust of the drying components 7. The drying components 6 are used to dry the gas introduced into the drying components 6. The humidity inside the box 2 can be monitored and controlled to ensure that the humidity of the gas inside the box 2 is kept in a low range, which can ensure that the water vapor inside the clamping component 5 maintains a high evaporation rate.

[0022] like Figure 1 and Figure 3As shown, the shock-absorbing component 3 includes a trapezoidal base 31, a mounting plate 32, and a connecting rod 36. The trapezoidal base 31 is installed at the bottom center of the housing 2. The cross-section of the trapezoidal base 31 is trapezoidal, and the inclined sides on both sides of the trapezoid can change the flow direction of the gas sprayed from the drying component 7, causing the airflow to flow upward. Several sets of guide grooves 33 are symmetrically arranged on the trapezoidal base 31. Sliding blocks 34 are slidably fitted in the guide grooves 33. The sliding blocks 34 are connected to the mounting plate 32 through the connecting rod 36. A limit ring 35 is installed on the top of the guide groove 33. The connecting rod 36 is slidably fitted with the limit ring 35, which can restrict the sliding blocks 34 in the guide grooves 33. The sliding mechanism prevents the sliding block 34 from being pulled out of the guide groove 33 when the wind is strong. A first spring 37 is provided between the sliding block 34 and the limiting ring 35. The first spring 37 is rotatably engaged with the connecting rod 36. When the mounting plate 32 moves upward, the elastic force of the first spring 37 can limit the upward range of the mounting plate 32. A second spring 38 is provided between the sliding block 34 and the bottom of the guide groove 33 to provide support for the sliding block 34. The two sides of the mounting plate 32 are connected to the top of the trapezoidal base 31 by a flexible windproof cloth 39, which will not affect the airflow passing through the side of the trapezoidal base 31 and will not interfere with the airflow direction. The top of the housing 2 is rotatably equipped with a door 9, which facilitates the retrieval of the clamping assembly 5.

[0023] The clamping assembly 5 includes a fixing plate 51, a ring 52, and an EP tube 53. The fixing plate 51 is installed on the top of the magnetic metal block 4. Several sets of rings 52 are arranged on both sides of the fixing plate 51. An EP tube 53 is installed inside the ring 52. The EP tube 53 is used to hold magnetic beads. All the magnetic beads can be accessed by removing the fixing plate 51. The opening of the EP tube 53 faces upward. The airflow passing through the bottom of the EP tube 53 will conduct heat to the EP tube 53, causing the temperature inside the EP tube 53 to rise. Under the action of the airflow, the liquid inside the EP tube 53 evaporates quickly and flows out from the opening of the EP tube 53.

[0024] The magnetic metal block 4 has a trapezoidal cross-section, and the width of the upper surface of the magnetic metal block 4 is greater than the width of the lower surface. The ring 52 is inclined downward, which allows the EP tube 53 to remain parallel to the side of the magnetic metal block 4. Under the interference of the inclined surface of the magnetic metal block 4, the gas flows to the top two sides of the box 2 and can enter the drying component 6 under the power of the drying component 7.

[0025] like Figure 1 and Figure 2As shown, the drying assembly 6 includes a gas collection box 61, a perforated plate 63, and a water-absorbing filling material 64. The gas collection box 61 is installed on both sides of the top of the housing 2. A through groove is provided on the top side of the gas collection box 61 away from the housing 2. A grid plate 62 is installed in the through groove. A temperature sensor 10 is installed on the grid plate 62. The temperature sensor 10 is used to collect the temperature of the air passing through the grid plate 62 and send the temperature signal to the control module 8. The control module 8 is installed on the housing 2 and can control the output power of the drying assembly 7 through the feedback signal information. The rate is adjusted to keep the temperature inside the chamber 2 stable. Several sets of perforated plates 63 are installed inside the gas collection box 61. Water-absorbing filling material 64 is filled between the perforated plates 63. The water-absorbing filling material 64 can adsorb the water vapor in the gas, so that the air becomes dry after passing through the water-absorbing filling material 64. The bottom of the gas collection box 61 is provided with a through hole 69. The dried gas can be discharged through the through hole 69 for reuse. The gas can be reused after being dried by the gas collection box 61, which can reduce the energy consumption of the drying component 7 during the drying process.

[0026] A first electric heating module 65 is provided between adjacent groups of water-absorbing filling materials 64. The first electric heating module 65 can heat the gas passing through the water-absorbing filling material 64. A humidity sensor 68 is installed at the bottom of the gas collection box 61. The humidity sensor 68 is used to collect the air humidity in the gas collection box 61 and send the humidity signal to the control module 8. The humidity sensor 68 can detect the air humidity sprayed from the drying component 7 in real time. The first electric heating module 65 heats the gas passing through the water-absorbing filling material 64. The high-temperature gas can carry away the water vapor in the water-absorbing filling material 64. A solenoid valve 66 is installed on the through hole 69. An exhaust pipe 67 is connected to the side of the gas collection box 61 near the box body 2. By closing the solenoid valve 66, the exhaust pipe 67 is opened. The high-temperature gas discharges water vapor from the exhaust pipe 67 to the outside of the box body 2. This can greatly reduce the water content in the water-absorbing filling material 64 and keep the air humidity in the box body 2 at a low level. This can realize continuous drying operation without replacing the water-absorbing filling material 64.

[0027] The drying assembly 7 includes a support plate 71, a drive motor 72, and a partition plate 75. The top of the partition plate 75 is connected to the air collection box 61, and the bottom of the partition plate 75 is connected to the housing 2. An air outlet groove is provided on the partition plate 75, and several sets of baffles 76 are rotatably installed within the air outlet groove. Adjusting the rotation direction of the baffles 76 adjusts the air outlet angle. A closed cavity is formed between the partition plate 75, the air collection box 61, and the housing 2. Gas discharged through the through hole 69 enters the closed cavity. Support plates 71 are installed on both sides of the bottom of the housing 2. Several sets of through holes are provided on the support plates 71, and various air intakes are installed on the support plates 71. A drive motor 72 is equipped with fan blades at its output end. The drive motor 72 drives the fan blades to rotate, which allows the dried air in the air collection box 61 to be discharged through the air outlet groove on the partition 75. A second electric heating module 74 is installed between the drive motor 72 and the partition 75. The second electric heating module 74 can heat the discharged air. A mesh cover 73 is installed on the drive motor 72 to prevent the fan blades from damaging the second electric heating module 74. The hot air discharged from the air outlet groove on the partition 75 can dry the magnetic beads in the EP tube 53 through the turbulence of the shock absorption component 3 and the magnetic metal block 4.

[0028] like Figure 4 As shown, the control module 8 includes a signal processing module 81, a temperature signal acquisition module 82, a humidity signal acquisition module 83, and an adjustment module 84. The temperature signal acquisition module 82 receives the temperature signal sent by the temperature sensor 10 and sends it to the signal processing module 81. The humidity signal acquisition module 83 receives the humidity signal sent by the humidity sensor 68 and sends it to the signal processing module 81. The signal processing module 81 processes the data sent by the temperature signal acquisition module 82 and the humidity signal acquisition module 83 and compares it with preset data within the signal processing module 81. After comparing the data, the signal processing module 81 sends a feedback signal to the adjustment module 84. The adjustment module 84 and the first electric heating module 6... 5. The terminals of the solenoid valve 66 and the second electric heating module 74 are connected. When the temperature sensor 10 reports that the temperature inside the housing 2 is lower than the preset temperature of the signal processing module 81, the adjustment module 84 increases the output power of the second electric heating module 74, thereby increasing the temperature of the gas discharged from the air outlet groove on the partition 75. When the humidity sensor 68 reports that the humidity inside the gas collection box 61 is higher than the preset humidity of the signal processing module 81, the adjustment module 84 powers on the first electric heating module 65 and closes the solenoid valve 66, so that the first electric heating module 65 heats the gas passing through the water-absorbing filling material 64, causing water vapor to be discharged from the exhaust pipe 67 outside the housing 2, thereby keeping the humidity inside the housing 2 and the gas collection box 61 at a low level.

[0029] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

[0030] All standard parts used in this invention can be purchased from the market, and irregular parts can be customized according to the description and drawings. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts and equipment adopt conventional models in the prior art, and the circuit connection adopts conventional connection methods in the prior art, which will not be described in detail here.

[0031] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0032] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A timed and temperature-controlled magnetic bead drying device, comprising a housing, a magnetic metal block, a clamping assembly, and a drying assembly, characterized in that, The housing is mounted on a base plate, on which a shock-absorbing assembly is installed. A magnetic metal block is fixedly mounted on the shock-absorbing assembly, which dampens the magnetic metal block. A clamping assembly is mounted on the magnetic metal block. Drying assemblies are installed on both sides of the bottom of the housing, and drying assemblies are installed on both sides of the top of the housing. The drying assemblies are connected to the drying assemblies. The shock-absorbing assembly includes a trapezoidal base, a mounting plate, and a connecting rod. The trapezoidal base is installed at the center of the bottom of the housing. The trapezoidal base has a trapezoidal cross-section, and the inclined sides on both sides of the trapezoid can change the flow direction of the gas ejected from the drying assembly, causing the airflow to flow upwards. Several sets of guide grooves are symmetrically arranged on the trapezoidal base, and these guide grooves slide together. The device includes a sliding block connected to a mounting plate via a connecting rod. A limit ring is installed at the top of the guide groove, and the connecting rod slides in conjunction with the limit ring. A first spring is provided between the sliding block and the limit ring, and the first spring rotates in conjunction with the connecting rod. A second spring is provided between the sliding block and the bottom of the guide groove. The sides of the mounting plate are connected to the top of the trapezoidal base via a flexible windproof cloth. The clamping assembly includes a fixing plate, rings, and EP tubes. The fixing plate is installed on the top of the magnetic metal block. Several sets of rings are provided on both sides of the fixing plate, and EP tubes are installed inside the rings with their openings facing upwards. The magnetic metal block has a trapezoidal cross-section, and the rings are inclined downwards.

2. The timed temperature-controlled magnetic bead drying device according to claim 1, characterized in that, A door is rotatably installed on the top of the box.

3. The timed temperature-controlled magnetic bead drying device according to claim 2, characterized in that, The drying assembly includes a gas collection box, perforated plates, and absorbent filling material. The gas collection box is installed on both sides of the top of the chamber. A through groove is provided on the top side of the gas collection box away from the chamber. A grid plate is installed in the through groove. A temperature sensor is installed on the grid plate. The temperature sensor is used to collect the temperature of the air passing through the grid plate and send the temperature signal to the control module. The control module is installed on the chamber. Several sets of perforated plates are installed inside the gas collection box. Absorbent filling material is filled between the perforated plates. Through holes are provided at the bottom of the gas collection box.

4. The timed temperature-controlled magnetic bead drying device according to claim 3, characterized in that, A first electric heating module is provided between the water-absorbing filling materials of adjacent groups. A humidity sensor is installed at the bottom of the air collection box. The humidity sensor is used to collect the air humidity in the air collection box and send the humidity signal to the control module. A solenoid valve is installed on the through hole. An exhaust pipe is connected to the side of the air collection box near the box body.

5. The timed temperature-controlled magnetic bead drying device according to claim 4, characterized in that, The drying assembly includes a support plate, a drive motor, and a partition. The top of the partition is connected to the air collection box, and the bottom of the partition is connected to the housing. An air outlet groove is provided on the partition, and several sets of baffles are rotatably installed in the air outlet groove. Support plates are installed on both sides of the bottom of the housing, and several sets of through holes are provided on the support plates. A drive motor is installed on the support plate, and a fan blade is installed at the output end of the drive motor. A second electric heating module is installed between the drive motor and the partition, and a mesh cover is installed on the drive motor.

6. The timed temperature-controlled magnetic bead drying device according to claim 5, characterized in that, The control module includes a signal processing module, a temperature signal acquisition module, a humidity signal acquisition module, and an adjustment module. The temperature signal acquisition module receives temperature signals from the temperature sensor and sends them to the signal processing module. The humidity signal acquisition module receives humidity signals from the humidity sensor and sends them to the signal processing module. The signal processing module processes the data sent by the temperature and humidity signal acquisition modules and compares them with preset data within the signal processing module. After comparing the data, the signal processing module sends a feedback signal to the adjustment module. The adjustment module is connected to the terminals of the first heating module, the solenoid valve, and the second heating module.

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