一种校正测序期间超前量的方法
By correcting secondary lead in high-throughput sequencers and utilizing parameter estimation and signal correction methods, sequencing read length and accuracy were improved, solving the signal bias problem in existing technologies and achieving higher sequencing accuracy.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- CYGNUS BIOSCI BEIJING CO LTD
- Filing Date
- 2016-11-16
- Publication Date
- 2026-07-17
AI Technical Summary
Existing high-throughput sequencers cannot completely eliminate sequencing errors, especially signal deviations caused by secondary lead, which affect sequencing accuracy.
By correcting secondary lead during sequencing, parameter estimation and signal correction methods are used, combined with the sequencing signal of a reference polynucleotide, to correct the sequencing signal of the target polynucleotide. Sequencing reactions are performed using alternating reaction solutions, and correction instructions are provided via computer-readable media.
It significantly improves sequencing read length and accuracy, accurately reads sequence information of about 350bp, corrects single-color and two-color signals, and does not affect the normal sequence of sequencing reactions.
Smart Images
Figure CN116083547B_ABST