A fungicidal composition containing copper octanoate and fluazinam

Abstract

The present invention provides a fungicidal composition containing copper octanoate and fluazinam, which contains an effective amount of (a) copper octanoate and (b) fluazinam, the weight ratio of the copper octanoate and the fluazinam being 100:1-1:20. The present invention also relates to the use of the fungicidal composition containing copper octanoate and fluazinam for controlling phytopathogenic fungi and bacteria; the present invention also particularly relates to a composition for controlling soil-borne diseases of plants and a method for controlling soil-borne diseases.

Description

Technical Field

[0001] This invention relates to a bactericidal composition containing copper octanoate and fluazinam; it also relates to a method for preventing or controlling plant pathogenic fungi and bacteria. Background Technology

[0002] Copper octanoate is a saturated fatty acid that combines copper ions and octanoic acid. It has contact toxicity and can be used as a fungicide and bactericide on leaves to control fungal and bacterial diseases in lawns, nut trees, landscape crops, and various fruit and vegetable crops in fields and greenhouses.

[0003] However, a well-known problem is that relatively high concentrations and high application rates per hectare are required to produce satisfactory results. But any increase in effectiveness comes at the cost of plant tolerance.

[0004] Ecological risk assessment studies have shown that copper products, typically applied at high rates, may be toxic to birds, mammals, fish, and other aquatic species ("Reregistration Eligibility Decision (RED) for Coppers", EPA 738-R-06-020, July 2006). Therefore, although copper is a useful agent for controlling harmful organisms in various environments, it is desirable to minimize the amount of copper applied.

[0005] Fungicides are used to protect plants from pathogenic fungi and bacteria. However, no single fungicide is effective in all situations. Repeated use of a single fungicide often leads to tolerance to that fungicide. There remains a need to develop new fungicidal compositions that are effective against broad-spectrum fungi and bacteria, have lower toxicity, higher selectivity, and can be applied at lower dose rates to reduce or avoid adverse environmental or toxicological effects while still allowing for effective prevention or control of pathogenic fungi and bacteria. Summary of the Invention

[0006] This invention provides novel bactericidal compositions containing copper octanoate and fluazinam that have advantages over known compounds in at least some respects. A synergistic effect occurs when the activity of two or more compounds exceeds the activity of the compounds when used alone.

[0007] The present invention provides a bactericidal composition containing copper octanoate and fluazinam, wherein the composition contains effective amounts of (a) copper octanoate and (b) fluazinam.

[0008] The present invention also relates to a bactericidal composition containing copper octanoate and fluazinam, comprising effective amounts of (a) copper octanoate and (b) fluazinam, and at least one agriculturally suitable carrier.

[0009] The present invention also relates to the use of the aforementioned bactericidal composition containing copper octanoate and fluazinam for controlling plant pathogenic fungi and bacteria.

[0010] The present invention also relates to a method for controlling plant pathogenic fungi and bacteria, comprising applying the fungicidal composition of the present invention containing copper octanoate and fluazinam to plants, plant parts, seeds, fruits or soil in which plants grow.

[0011] In terms of reducing application rates and improving the activity spectrum of known compounds, the fungicidal composition of the present invention containing copper octanoate and fluazinam exhibits a synergistic effect of improving the activity against plant pathogens while reducing the total amount of active compounds applied.

[0012] Compositions and formulations

[0013] The present invention provides a bactericidal composition containing copper octanoate and fluazinam, wherein the composition contains effective amounts of (a) copper octanoate and (b) fluazinam.

[0014] The weight ratio of copper octoate and fluazinam is 100:1-1:20, preferably 50:1-1:10, more preferably 50:1-1:5, more preferably 40:1-1:1, and even more preferably 20:1-1:1.

[0015] The bactericidal composition containing copper octanoate and fluazinam typically contains effective amounts of (a) copper octanoate and (b) fluazinam, and at least one agriculturally suitable carrier.

[0016] The bactericidal composition containing copper octanoate and fluazinam according to the present invention typically contains 5-99% by weight, preferably 5-90% by weight, more preferably 5-80% by weight of copper octanoate and fluazinam.

[0017] Carriers typically improve the suitability of compounds for, for example, plants, plant parts or seeds, help the active ingredient reach the site to be treated, and store, transport or process the active ingredient to make it easier to apply.

[0018] The carrier is typically an inert solid or liquid. The amount of carrier is typically in the range of 1-95% by weight, preferably 10-95% by weight, and more preferably 20-95% by weight of the bactericidal composition.

[0019] Examples of suitable solid carriers include, but are not limited to, inorganic substances such as bentonite, montmorillonite, kaolinite, diatomaceous earth, kaolin, talc, clay, gypsum, calcium carbonate, amorphous silica, ammonium sulfate, etc.; and plant organic substances such as soybean flour, wood flour, sawdust, wheat flour, lactose, sucrose, glucose, etc.

[0020] Examples of suitable liquid carriers include, but are not limited to, water, organic solvents, and combinations thereof. Examples of suitable solvents include polar and nonpolar organic chemical liquids, such as water, petroleum ether, vegetable oils, methyl ethyl ketone, cyclohexanone, amyl acetate, 2-butanone, butenyl carbonate, cyclohexane, cyclohexanol, alkyl acetate, diacetone alcohol, diethanolamine, diethylene glycol, diethylene glycol butyl ether, diethylene glycol ethyl ether, diethylene glycol methyl ether, N,N-dimethylformamide, dimethyl sulfoxide, dipropylene glycol, dipropylene glycol methyl ether, dipropylene glycol dibenzoate, dipropylene glycol, alkylpyrrolidone, ethyl acetate, 2-ethylhexanol, ethylene carbonate, 2-heptanone, ethyl lactate, ethylene glycol, ethylene glycol butyl ether, ethylene glycol methyl ether, γ-butyrolactone, glycerol, glyceryl acetate, glyceryl diacetate, triethyl... Glyceryl esters, hexanediol, isopentyl acetate, isobornyl acetate, isooctane, isophorone, isopropyl myristate, lactic acid, laurylamine, isopropyl acetone, methoxypropanol, methyl isopentyl ketone, methyl isobutyl ketone, methyl laurate, methyl octanoate, methyl oleate, n-hexane, n-octylamine, octadecanoic acid, octylamine acetate, oleic acid, oleyleneamine, polyethylene glycol, propionic acid, propyl lactate, propylene carbonate, propylene glycol, propylene glycol methyl ether, triethyl phosphate, triethylene glycol, paraffin, mineral oil, ethyl acetate, amyl acetate, butyl acetate, propylene glycol methyl ether, diethylene glycol methyl ether, ethanol, isopropanol, and higher molecular weight alcohols such as pentanol, tetrahydrofuranol, hexanol, octanol, ethylene glycol, propylene glycol, glycerol, etc.

[0021] The bactericidal composition containing copper octanoate and fluazinam may further comprise one or more acceptable adjuvants, such as one or more surfactants, which are commonly used in the formulation of compositions (e.g., agrochemical compositions). The amount of surfactant is typically from 0% to 80% by weight of the composition.

[0022] Adjuvants can be used alone or in combination, depending on the purpose and considering the formulation, preparation method, etc. Adjuvants that may be mentioned are typically surfactants used for emulsifying, dispersing, spreading, and / or wetting purposes of crops.

[0023] Nonionic surfactants, such as polyoxyethylene castor oil, polyoxyethylene alkyl ethers, polyoxyethylene alkylphenyl ethers, polyoxyethylene dialkylphenyl ethers, polyoxyethylene polyoxypropylene block polymers, alkyl polyoxyethylene polyoxypropylene block polymer ethers, alkylphenyl polyoxyethylene polyoxypropylene block polymer ethers, polyoxyethylene alkylamines, polyoxyethylene fatty acid amides, polyoxyethylene diphenyl ethers, polyoxyethylene benzylphenyl ethers, as well as sorbitol fatty acid esters, polyoxyethylene sorbitol fatty acid esters, sucrose fatty acid esters, polyoxyethylene fatty acid esters, polyoxyethylene fatty acid diesters, etc.

[0024] Anionic surfactants, such as naphthalene sulfonate, dialkyl naphthalene disulfonate, alkyl diphenyl ether disulfonate, lignin sulfonate, polyoxyethylene alkylphenyl ether sulfonate, polyoxyethylene alkyl ether sulfosuccinate half ester, fatty acid salts, polyoxyethylene alkyl ether phosphates, alkyl sulfates, polyoxyethylene alkyl ether sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene benzylphenyl ether sulfates, polyoxyethylene styrene phenyl ether sulfates, polyoxyethylene polyoxypropylene block polymer sulfates, alkyl sulfonates, dialkyl sulfosuccinate sulfonate, alkylbenzene sulfonate, polyoxyethylene phenyl ether phosphates, polyoxyethylene dialkylphenyl ether phosphates, polyoxyethylene benzyl phenyl ether phosphates, polyoxyethylene styrene phenyl ether phosphates, polyoxyethylene polyoxypropylene block polymer phosphates, alkyl phosphates, sodium tripolyphosphate, etc.

[0025] Adhesives, such as polyvinyl alcohol, gum arabic, bentonite, etc.;

[0026] Disintegrants, such as sodium CMC or croscarmellose sodium;

[0027] Stabilizers, such as hindered phenolic antioxidants, or benzotriazole or hindered amine UV absorbers, etc.

[0028] pH adjusters, such as citric acid, phosphoric acid, acetic acid, or sodium hydroxide;

[0029] Fungicides, antifungals and preservatives used for industrial purposes, such as 1,2-benzothiazolin-3-one;

[0030] Thickeners, such as xanthan gum, guar gum, sodium CMC, gum arabic, polyvinyl alcohol, or montmorillonite;

[0031] Defoamers, such as silicone compounds;

[0032] Antifreeze, such as propylene glycol, or ethylene glycol, etc.

[0033] These adjuvants are not limited to the above. The choice of adjuvants is related to the intended method of application and / or physical properties of the bactericidal composition of the present invention containing copper octanoate and fluazinam. The bactericidal composition of the present invention containing copper octanoate and fluazinam can be in any conventional form and can be prepared by known methods. The active compounds of the present invention can be present in suspended, emulsified, or dissolved forms. For example, emulsifiable concentrates, emulsions, suspensions, water-dispersible and water-soluble particles, water-dispersible and water-soluble powders, gels, powders, granules, and ULV solutions.

[0034] The bactericidal composition of the present invention, containing copper octanoate and fluazinam, can be provided to the end user as a ready-to-use formulation, i.e., the composition can be applied directly to plants or seeds using a suitable device such as a sprayer or powderer. Alternatively, the composition can be provided to the end user in the form of a concentrate, which must be diluted before use, preferably with water.

[0035] To provide optimal results, the bactericidal composition of the present invention, containing copper octanoate and fluazinam, can be processed into various formulations with fertilizers, or applied together with or mixed with fertilizers. Suitable fertilizers include those containing one or more macroelements such as nitrogen, phosphorus, and potassium, or one or more microelements such as copper, iron, manganese, zinc, boron, calcium, magnesium, and sulfur, or mixtures of one or two fertilizers containing humic acid and amino acids.

[0036] Pathogens and diseases

[0037] The fungicidal composition of the present invention, containing copper octanoate and fluazinam, is used to control the following plant fungal and bacterial diseases:

[0038] The fungicidal composition of the present invention, containing copper octanoate and fluazinam, can be used in crop protection to control unwanted fungi such as Chytridiomycetes, Zygomycetes, Ascomycetes, Basidiomycetes, Deuteromycetes, and / or Oomycetes. More preferably, it is used to prevent or control plant diseases caused by Deuteromycetes and Oomycetes, for example, diseases caused by the following fungal pathogens:

[0039] Plant pathogenic fungi that cause seed-borne and soil-borne rot and wilting diseases, as well as seedling diseases, include: * *Alternaria* species (e.g., *Alternaria brassicicola*), * *Aphanomyces* species (e.g., *Aphanomyces euteiches*), * *Ascochyta* species (e.g., *Ascochyta lentis*), * *Aspergillus* species (e.g., *Aspergillus flavus*), * *Cladosporium* species (e.g., *Cladosporium herbarum*), and * *Cochliobolus* species (e.g., *Cochliobolus sativus*). (Conidia forms: *Drechslera*, *Helminthosporium*)), *Colletotrichum* species (e.g., *Colletotrichum coccodes*), *Fusarium* species (e.g., *Fusarium culmorum*), *Gibberella* species (e.g., *Gibberella zeae*), *Macrophomina* species (e.g., *Macrophomina phaseolina*), *Microdochium* species (e.g., *Microdochium nivale*), *Monographella* species (e.g., *Monographella nivalis*), *Penicillium* species (e.g., *Penicillium expansum*) Phoma species (e.g., Phoma galingam), Phopsis species (e.g., Phopsis sojae), and Phytophthora species (e.g., Phytophthora)Species of the genera *Cavorum*, *Pyrenophora* (e.g., *Pyrenophora graminea*), *Pyricularia* (e.g., *Pyricularia oryzae*), *Pythium* (e.g., *Pythium ultimum*), *Rhizoctonia* (e.g., *Rhizoctonia solani*), *Rhizopus* (e.g., *Rhizopus oryzae*), *Sclerotium* (e.g., *Sclerotium rolfsii*), *Septoria* (e.g., *Septoria nodorum*), and *Typhula* (e.g., *Typhula* var. *sarcosporum*). (incarnata), Verticillium species (e.g., Verticillium dahliae);

[0040] Pathogenic fungi causing root and stem diseases include: * *Corticium* species (e.g., *Corticium graminearum*), * *Fusarium* species (e.g., *Fusarium oxysporum*), * *Gaeumannomyces* species (e.g., *Gaeumannomyces graminis*), * *Plasmodiophora* species (e.g., *Plasmodiophora brassicae*), * *Rhizoctonia* species (e.g., *Rhioctonia solani*), * *Sarocladium* species (e.g., *Sarocladium oryzae*), * *Sclerotium* species (e.g., *Sclerotium oryzae*), and * Tapesia* species (e.g., *Tapesia*). acuformis), species of the genus Thieviopsis (e.g., Thieviopsis basicola);

[0041] Pathogenic fungi that cause fruit rot include: Aspergillus species (e.g., Aspergillus flavus), Botrytis species (e.g., Botrytis cinerea), Penicillium species (e.g., Penicillium expansum or Penicillium purpurogenum), Rhizopus species (e.g., Rhizopus stolonifer), Sclerotinia species (e.g., Sclerotinia sclerotiorum), and Verticilium species (e.g., Verticilium alboatrum).

[0042] Pathogenic fungi that cause diseases of flowers and seeds: for example, Botrytis species (e.g., Botrytis cinerea);

[0043] Pathogenic fungi that cause plant tuber diseases: for example, Rhizoctonia species (e.g., Rhizoctonia solani) and Helminthosporium species (e.g., Helminthosporium solani).

[0044] Diseases caused by pathogens of the class Oomycetes: for example, Albugo species (e.g., Algubo candida), Bremia species (e.g., Bremia lactucae), Peronospora species (e.g., Peronosporapisi or P. brassicae), Phytophthora species (e.g., Phytophthora infestan), Plasmopara species (e.g., Plasmopara viticola), Pseudoperonospora species (e.g., Pseudoperonospora humuli or Pseudoperonospora Cubensis), and Pythium species (e.g., Pythium ultimum).

[0045] Pathogenic fungi that cause powdery mildew include: Blumeria species (e.g., Blumeria graminis in the Poaceae family), Podosphaera species (e.g., Podosphaera leucotricha), Sphaerotheca species (e.g., Sphaerotheca fuliginea), and Uncinula species (e.g., Uncinula necator in grapes).

[0046] Pathogenic fungi that cause rust include: *Gymnosporangium* species (e.g., *Gymnosporangium sabinae*), *Hemileia* species (e.g., *Hemileia vastatrix*), *Phakopsora* species (e.g., *Phakopsora pachyrhizi* or *Phakopsora meibomiae*), *Puccinia* species (e.g., *Puccinia recondite*, *Puccinia graminis*, or *Puccinia striiformis*), and *Uromyces* species (e.g., *Uromyces appendiculatus*).

[0047] Diseases caused by the following bacterial pathogens:

[0048] Xanthomonas species (e.g., Xanthomonas campestris pv. oryzae);

[0049] Species of the genus Pseudomonas (e.g., Pseudomonas syringaepv. lachrymans, a pathogenic species of cucumber);

[0050] Erwinia species (e.g., Erwinia amylovora).

[0051] Methods and uses

[0052] The bactericidal composition of the present invention containing copper octanoate and fluazinam can be used to protect seeds, germinating seeds, seedlings, plants, plant parts, fruits, harvested products and / or the soil in which plants grow from the invasion of plant pathogenic fungi and bacteria.

[0053] The bactericidal composition of the present invention containing copper octanoate and fluazinam is particularly effective against root rot, clubroot, scab, leaf spot, powdery mildew, anthracnose, bacterial wilt, late blight, clubroot, canker, bacterial angular leaf spot, bacterial basal rot, bacterial wilt, bacterial streak, and bacterial leaf blight.

[0054] The fungicidal composition of the present invention containing copper octanoate and fluazinam is particularly effective against the following fungal plant diseases: late blight of tomato, root rot of ginger, clubroot of Chinese cabbage, scab of citrus, leaf spot of banana, stem base rot of corn, sheath blight of rice, downy mildew of cucumber, wilt of watermelon, downy mildew of grape, rice false smut, anthracnose of pepper, anthracnose of grape, anthracnose of cucumber, leaf spot of celery, powdery mildew of strawberry, downy mildew of lettuce, gray mold of celery, and downy mildew of onion.

[0055] The bactericidal composition of the present invention containing copper octanoate and fluazinam is particularly effective against the following bacterial plant diseases: bacterial leaf spot of peach trees, wildfire disease of tobacco, bacterial wilt of eggplant, bacterial angular leaf spot of cucumber, bacterial leaf streak of rice, bacterial basal rot of rice, bacterial wilt of maize, bacterial leaf streak of rice, lychee canker, peach canker, bacterial leaf spot of apricot, bacterial angular leaf spot of cotton, and bacterial leaf blight of cucumber.

[0056] The present invention also relates to a method for preventing or controlling plant pathogenic fungi and bacteria by applying the fungicidal composition of the present invention containing copper octanoate and fluazinam to seeds, germinating seeds, seedlings, plants, plant parts, fruits, harvested products and / or the soil on which the plants grow.

[0057] The present invention also relates to a method for preventing or controlling plant pathogenic fungi and bacteria, the method comprising applying the fungicidal composition of the present invention in an effective and plant-compatible amount to seeds, germinated seeds, seedlings, plants, plant parts, fruits, harvested products and / or the soil on which the plants grow by means of seed treatment, foliar application, stem application, soaking, dripping, watering, spraying, misting, dusting, dispersing or fumigation.

[0058] "Prevention or control" includes protective treatments, therapeutic treatments, and eradication treatments for plant pathogenic fungi and bacteria.

[0059] Seed treatment

[0060] The present invention also relates to the use of the fungicidal composition containing copper octanoate and fluazinam in the treatment of seeds to protect seeds and resulting plants from fungal and bacterial diseases.

[0061] The present invention also relates to a method for protecting seeds and germinating plants from fungal and bacterial diseases by treating seeds with the fungicidal composition of the present invention containing copper octanoate and fluazinam.

[0062] The present invention also relates to treating seeds with copper octanoate and fluazinam at different times. When seeds have been treated with copper octanoate and fluazinam at different time points, copper octanoate and fluazinam can exist on the seeds in different layers.

[0063] The fungicidal composition of the present invention, containing copper octanoate and fluazinam, is suitable for protecting seeds of any plant variety used in agriculture, greenhouses, forestry, or horticulture, particularly in the form of cereal seeds (e.g., wheat, barley, rye, millet, and oats), corn, cotton, soybeans, rice, potatoes, sunflowers, coffee, tobacco, low-erucic acid rapeseed, rapeseed, sugar beets (e.g., beets and forage beets), peanuts, vegetables (e.g., tomatoes, cucumbers, legumes, cruciferous vegetables, onions, and lettuce), fruit trees, lawns, and ornamental plants. More preferably, it is suitable for seeds of cereals (e.g., wheat, barley, rye, and oats), corn, soybeans, cotton, rapeseed, vegetables, and rice.

[0064] The fungicidal composition of the present invention, containing copper octanoate and fluazinam, can be applied to seeds in any physiological state, including dormant seeds, budding seeds, pre-germinating seeds, and seeds that have sprouted roots and leaves. Generally, seeds can be treated at any point between harvest and sowing.

[0065] The application methods of the fungicidal composition containing copper octanoate and fluazinam on plant propagation materials, especially seeds, include seed dressing, coating, granulation, powdering, soaking, and application into the furrows of the propagation material. Preferably, it is applied to the plant propagation material by methods that do not induce germination, such as seed dressing, granulation, coating, and powdering.

[0066] Typically, the fungicidal compositions of the present invention, containing copper octanoate and fluazinam, are applied to seeds in a suitable formulation. Suitable formulations and methods for seed treatment are known to those skilled in the art. Examples of suitable formulations commonly used for treating plant seeds include solutions, emulsions, suspensions, powders, foams, and slurries.

[0067] The formulation may be a ready-to-use formulation or a concentrate that must be diluted before use. The bactericidal composition of the present invention can be used directly or after dilution with water to treat a variety of different types of seeds. For example, the concentrate or formulation obtained therefrom by dilution with water can be used for coated cereal seeds, such as wheat, barley, rye, oats and triticale, as well as corn, rice, rapeseed, beans, cotton, sunflower, beet seeds or a wide variety of different vegetable seeds.

[0068] The amount of the fungicidal composition of the present invention containing copper octanoate and fluazinam applied to the seeds is typically such that it provides optimal protection to the seeds and germinating plants from fungal and bacterial diseases without harming the seeds and germinating plants.

[0069] Seeds treated with the fungicidal composition containing copper octanoate and fluazinam of the present invention are protected from fungal and bacterial diseases after germination, not only the seeds themselves but also the germinating plants. This eliminates the need for treatment of crops at or immediately after sowing, and reduces or eliminates the need for additional pesticide application.

[0070] Soil treatment

[0071] Diseases caused by pathogenic fungi and bacteria in the soil (soil-borne diseases) are difficult to control by foliar application of chemical agents. In soil-borne diseases, it is difficult to eradicate the pathogenic microorganisms, and when the soil is infected with soil-borne diseases, it tends to inhibit plant growth in the long term.

[0072] The present invention provides compositions and methods for controlling soil-borne diseases, which reduce the severity of plant diseases in fields affected by such soil-borne diseases.

[0073] The present invention also provides a method for preventing and controlling harmful bacteria in soil, by applying the bactericidal composition of the present invention to the soil and / or directly applying it to the soil in contact with plant roots or to soil suitable for plant growth.

[0074] This invention is particularly effective against bacterial soil-borne diseases, represented by bacterial wilt, fungal soil-borne infectious diseases, represented by root rot, and filamentous fungal soil-borne infectious diseases, represented by Fusarium.

[0075] Methods of applying pesticides to the soil include, for example, diluting liquid pesticides in water or applying them directly to the roots of plants without dilution; spreading granules to the roots of plants; spraying powders or water-dispersible granules into the soil and mixing them with the soil before sowing; and diluting powders or water-dispersible granules and spraying them into planting holes or furrows before sowing or planting plants.

[0076] Soil application methods include applying pesticide compounds directly or indirectly to the soil and / or ground, such as drip application or drip irrigation (to the soil) or other methods such as soil injection or soil saturation. Other known soil application methods include furrow application and T-strip application.

[0077] Plants, plant parts and plant propagation material

[0078] The bactericidal composition of the present invention, containing copper octanoate and fluazinam, can treat all plants, plant parts, and plant propagation materials.

[0079] "Plant" refers to all plants and plant populations, including desirable and undesirable wild plants, cultivated plants, and plant varieties (whether or not protected by plant variety or plant breeder rights). Cultivated plants and plant varieties can be plants obtained through conventional propagation and cultivation methods, which may be supplemented by one or more biotechnological methods, such as the use of double haploids, protoplast fusions, random and directed mutations, molecular or genetic markers, or the use of bioengineering and genetic engineering methods.

[0080] "Plant parts" refers to all above-ground and underground parts and organs of a plant, such as buds, leaves, flowers and roots, including leaves, needles, stems, branches, flowers, fruiting bodies, fruits and seeds, as well as roots, bulbs and rhizomes.

[0081] "Plant propagation material" should be understood to refer to all plant parts capable of reproduction, such as seeds, which can be used to propagate the latter, and plant materials such as cuttings or tubers (e.g., potatoes). Therefore, the plant parts used herein include plant propagation material. Examples that may be mentioned include, for instance, seeds, roots, fruits, tubers, bulbs, rhizomes, and plant parts. Germination should be suppressed after sprouting from the soil or after emergence, and viable plants should also be considered. Young plants may be protected by whole or partial treatment with immersion before transplanting.

[0082] The bactericidal composition of the present invention containing copper octanoate and fluazinam can treat the following plants: major crop plants, such as Brassicaceae sp. (e.g., white cabbage, red cabbage, broccoli, cauliflower, Brussels sprouts, leafy greens, kohlrabi, radishes, as well as rapeseed, mustard, horseradish and watercress), Fabaceae sp. (e.g., legumes, peanuts), Solanaceae sp. (e.g., potatoes), Chenopodiaceae sp. (e.g., sugar beets, forage beets), Rutaceae sp. (e.g., lemons, oranges and grapefruits), cotton, flax, grapevines, fruits, vegetables, Ribesioidae sp., Cucurbitaceae sp. (e.g., cucumbers), Gramineae sp. (e.g., corn, turf, cereals such as wheat, rye, rice, barley, oats, millet and triticale), Asteraceae Useful and ornamental plants for garden and forest areas, including: sunflower, Juglandaceae, Betulaceae, Anacardiaceae, Fagaceae, Moraceae, Oleaceae, Actinidaceae, Lauraceae, Musaceae (e.g., banana trees and plantations), Rubiaceae (e.g., coffee), Theaceae, Sterculiaceae, Liliaceae, Asteraceae (e.g., lettuce), Umbelliferae, and Allium (e.g., leeks, onions).

[0083] application

[0084] In the bactericidal composition containing copper octanoate and fluazinam of the present invention, copper octanoate and fluazinam can be applied in combination / in combination, including separately, sequentially or simultaneously. Preferably, the combination of copper octanoate and fluazinam is in the form of a composition comprising copper octanoate and fluazinam.

[0085] The bactericidal composition of the present invention containing copper octanoate and fluazinam can be applied in the form of ready-to-use solutions, emulsions, water-based or oil-based suspensions, powders, wettable powders, pastes, soluble powders, soluble granules, broadcast granules, suspension concentrates, fertilizers, or microcapsules in polymeric substances.

[0086] The fungicidal composition of the present invention, containing copper octanoate and fluazinam, is applied in a conventional manner at an agronomically effective and substantially non-phytotoxic level, such as by watering, spraying, atomizing, spreading, dusting, foaming, or spreading. It can also be applied via ultra-low volume methods, drip irrigation systems, or immersion. The fungicidal composition of the present invention can also be applied to furrows or injected into the soil.

[0087] The amount of the bactericidal composition containing copper octanoate and fluazinam of the present invention applied to plants, plant parts, fruits, seeds or soil must be effective and plant-compatible.

[0088] "Effective and plant-compatible amount" means an amount that is agronomically effective and does not cause any obvious phytotoxic symptoms in the crop. This will depend on a variety of factors, such as the object of treatment (plant, plant part, fruit, seed, or soil), type of treatment (powdering, spraying, seed dressing), purpose of treatment (therapeutic and protective), type of plant pathogenic fungus or bacteria, developmental stage of the plant pathogenic fungus or bacteria, susceptibility of the plant pathogenic fungus or bacteria, crop growth stage, and environmental conditions.

[0089] This invention discloses a fungicide composition containing copper octanoate and fluazinam, which exhibits a synergistic effect when the activity of the two or more compounds exceeds that of the compounds when used alone. In terms of reducing application rates and improving the activity spectrum of known compounds, the fungicide composition of this invention containing copper octanoate and fluazinam exhibits a synergistic effect on improving the activity of plant pathogens while reducing the total amount of active compounds applied. Detailed Implementation

[0090] Biological test cases

[0091] Synergistic effects exist when the effect of a combination of active compounds exceeds the sum of the effects of each active compound when applied alone. The expected effect of a specific combination of two active compounds can be calculated using the so-called "Colby formula" (see SR Colby, "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations", Weeds 1967, 15, 20-22) as follows:

[0092]

[0093] X is the activity when active compound A is used at a dosage of mg / ha or a concentration of m ppm;

[0094] Y is the activity when active compound B is used at a dosage of ng / ha or a concentration of n ppm, expressed as a percentage of the untreated control;

[0095] E represents the activity when active compounds A and B are used at dosages of m and ng / ha or concentrations of m and n ppm.

[0096] If the actual observed activity (O) is greater than the expected activity (E), then the composition has a synergistic effect.

[0097] The following biological test examples are used to illustrate the present invention. However, the present invention is not limited to these embodiments.

[0098] Experiment 1: Bacterial angular leaf spot of cucumber

[0099] The technical grade copper octanoate and fluazinam were dissolved in acetone to prepare single-agent stock solutions, which were then diluted with an aqueous solution containing 0.1% Tween-80 to the required concentration.

[0100] The activity of the pesticide against bacterial angular leaf spot of cucumber was determined using a live pot method. The cucumber variety was Xintai Mici, planted in pots with a vegetable growing substrate, and prepared for use when the cucumbers reached the 2-leaf stage.

[0101] The potted seedlings were sprayed evenly with the foliage. The leaves of the plants to be inoculated were sprayed with each treatment agent until moist, and inoculated 24 hours later. Four replicates were performed, with 10 pots per replicate and one seedling per pot. A blank control was also included, containing no treatment (containing organic solvents and emulsifiers).

[0102] Adjust the bacterial suspension in the liquid culture medium to OD using sterile water. 600 The concentration was set to 0.8, and then the inoculation was evenly sprayed onto the cucumber leaves using an inoculation sprayer.

[0103] The inoculated potted cucumber seedlings were placed in a humidity-controlled incubator. Based on the disease incidence in the blank control, the inoculated leaves were graded. The grading method was as follows:

[0104] Disease severity is determined by the proportion of leaf area covered by lesions.

[0105] Level 0: No disease;

[0106] Grade 1: The area of ​​lesions accounts for less than 10% of the total leaf area;

[0107] Grade 3: Lesions cover 11%-25% of the entire leaf area;

[0108] Level 5: Lesions cover 26%-45% of the entire leaf area.

[0109] Level 7: Lesions cover 46%-65% of the entire leaf area;

[0110] Level 9: The lesion area accounts for more than 65% of the total leaf area.

[0111] The disease index and prevention efficacy are calculated using the following formulas.

[0112]

[0113] Table 1. The efficacy of the bactericidal composition of the present invention containing copper octanoate and fluazinam against bacterial angular leaf spot of cucumber.

[0114]

[0115] Table 1 clearly shows that the actual control efficacy of the bactericidal composition of the present invention containing copper octanoate and fluazinam against bacterial angular leaf spot of cucumber is higher than that calculated by the Colby formula, i.e., there is a synergistic effect.

[0116] Experiment 2: Powdery mildew in cucumbers

[0117] The technical grade copper octanoate and fluazinam were dissolved in acetone to prepare stock solutions for single-agent preparations, which were then diluted with an aqueous solution containing 0.1% Tween-80 to the required concentration.

[0118] The fungicidal activity of the agent against cucumber powdery mildew was determined using the live pot method. Potted cucumber seedlings with uniform growth at the two-leaf stage were selected for use.

[0119] Fresh spores from diseased cucumber leaves were washed off with a 0.1% Tween-80 aqueous solution and filtered through double-layered gauze to obtain a concentration of 5×10⁻⁶. 5 ~6×10 5 Prepare a spore suspension of 1 spore per mL for later use.

[0120] The potted seedlings were sprayed evenly with the spray until the leaves were completely moistened. The solution was allowed to air dry naturally before inoculation 24 hours later. Four replicates were performed, with 10 pots per replicate and one seedling per pot. A blank control was also included, containing no pesticide (but containing organic solvents and emulsifiers).

[0121] The sporangium suspension was evenly sprayed onto cucumber leaves using an inoculation sprayer. After inoculation, cucumber seedlings were moved to a greenhouse with a light intensity greater than 2000 lx for normal cultivation. An investigation was conducted 7 days later. Disease incidence was assessed at different treatment levels.

[0122] The grading method is as follows:

[0123] Level 0: No disease;

[0124] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0125] Grade 3: Lesions cover 6%-10% of the entire leaf area;

[0126] Level 5: Lesions cover 11%-20% of the entire leaf area;

[0127] Level 7: Lesions cover 21%-40% of the entire leaf area;

[0128] Level 9: The lesion area accounts for more than 40% of the total leaf area.

[0129] The disease index and prevention efficacy are calculated using the following formulas.

[0130]

[0131] Table 2. The efficacy of the fungicidal composition of the present invention containing copper octanoate and fluazinam against cucumber powdery mildew.

[0132]

[0133] Table 2 clearly shows that the actual control efficacy of the fungicidal composition of the present invention containing copper octanoate and fluazinam against cucumber powdery mildew is higher than the control efficacy calculated by the Colby formula, i.e., there is a synergistic effect.

[0134] Experiment 3: Banana leaf spot disease

[0135] The technical grade copper octanoate and fluazinam were dissolved in acetone to prepare single-agent stock solutions, which were then diluted with an aqueous solution containing 0.1% Tween-80 to the required concentration.

[0136] The activity of the pesticide against banana leaf spot disease was determined using a live pot method. Potted banana seedlings with 6-8 leaves were selected for use.

[0137] The pathogen used in the experiment (Curvularia natans leaf spot pathogen) was cultured on a suitable culture medium. After the mycelium had fully grown on the medium, the aerial mycelium was washed away with tap water. Conidia were cultured under 400nm fluorescent light. After 2-3 days, when conidia were produced, the conidia were washed off with distilled water and diluted to a concentration of 10×10. Under a low magnification microscope, a spore suspension with 80-100 conidia per field of view was prepared for use.

[0138] The potted seedlings were sprayed evenly with the foliage. Both sides of the leaves to be inoculated were sprayed with the respective treatment agents until moist. After the solution dried, the spore suspension was inoculated onto the banana leaves. 3-5 leaves were inoculated per banana seedling. Each treatment was repeated 4 times per pot. A blank control was set up, containing no agents (containing organic solvents and emulsifiers).

[0139] After inoculation, place the banana seedlings at 28°C. oCultured in a C-type incubator under humidity. Disease incidence was investigated at different treatment levels.

[0140] The grading method is as follows:

[0141] Level 0: No disease;

[0142] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0143] Grade 3: Lesions cover 6%-10% of the entire leaf area;

[0144] Level 5: Lesions cover 11%-30% of the entire leaf area;

[0145] Level 7: Lesions cover 31%-50% of the entire leaf area;

[0146] Level 9: The lesion area accounts for more than 50% of the total leaf area.

[0147] The disease index and prevention efficacy are calculated using the following formulas.

[0148]

[0149] Table 3. The efficacy of the fungicidal composition of the present invention containing copper octanoate and fluazinam against banana leaf spot disease.

[0150]

[0151] Table 3 clearly shows that the actual control efficacy of the fungicidal composition of the present invention containing copper octanoate and fluazinam against banana leaf spot disease is higher than the control efficacy calculated by the Colby formula, i.e., there is a synergistic effect.

[0152] Experiment 4: Peanut root rot

[0153] The technical grade copper octanoate and fluazinam were dissolved in acetone to prepare stock solutions for single-agent preparations, which were then diluted with an aqueous solution containing 0.1% Tween-80 to the required concentration.

[0154] The preserved peanut root rot pathogen (Fusarium solani) was inoculated into PDA medium and cultured in a greenhouse at 28°C for 5 days to activate it.

[0155] Place oat grains into Erlenmeyer flasks, soak in distilled water for 6 hours, then drain the water and autoclave at 120℃ for 20 minutes. Inoculate peanut root rot fungus (Fusarium solani) into the sterilized oat grains and incubate at 28℃ for 7 days. Shake the inoculation flasks twice a day to ensure that all oat grains are infected with the pathogen, thus obtaining infected oat grains.

[0156] Plant peanuts individually in pots, 3 peanuts per pot. Inoculate the peanuts with infected oat grains around them using the topsoil spreading method, then cover with a thin layer of soil and water. The inoculation amount is 10 grains per plant. Then place them in an incubator for cultivation. The incubator temperature is 28℃ during the day and 25℃ at night, with 12 hours of light / 12 hours of darkness.

[0157] Two days after sowing, the roots were drenched with the treatment agent. Each treatment consisted of 10 pots with 3 plants per pot, and the treatment was repeated 3 times. A control group was set up with no treatment (containing organic solvents and emulsifiers). Peanuts were watered once a day.

[0158] Observe peanut disease symptoms daily, and water peanuts once a day. Investigate root rot symptoms 75 days after planting. Investigate disease symptoms for each treatment in a tiered manner, using the following tiering method:

[0159] Grade 0: No lesions on the stem base and main rootlets;

[0160] Grade 1: A few lesions on the stem base and main root;

[0161] Grade 3: Numerous lesions on the stem base and main root, with the lesion area accounting for 1 / 4 to 1 / 2 of the total area of ​​the stem base and root;

[0162] Grade 5: Numerous and large lesions on the stem base and main root, covering 1 / 2 to 3 / 4 of the total area of ​​the stem base and roots;

[0163] Level 7: Patches of disease merge on the stem base and main root, forming a stem-encircling phenomenon, but the root system is not dead;

[0164] Level 9: Root necrosis, withered or dead above-ground parts of the plant.

[0165] Disease index = ∑(Representative value of disease severity level × Number of diseased plants at each level) × 100 / (Total number of plants surveyed × Representative value of the highest disease severity level)

[0166] Prevention and control effect = [(Control disease index - Treatment disease index) / Control disease index] × 100%

[0167]

[0168] Table 4. The efficacy of the fungicidal composition of the present invention containing copper octanoate and fluazinam against peanut root rot.

[0169]

[0170] Table 4 clearly shows that the actual control efficacy of the fungicidal composition of the present invention containing copper octanoate and fluazinam against peanut root rot is higher than that calculated by the Colby formula, i.e., there is a synergistic effect.

Claims

1. A bactericidal composition containing copper octanoate and fluazinam, characterized in that, The active ingredient consists of effective amounts of (a) copper octanoate and (b) fluazinam, wherein the weight ratio of copper octanoate to fluazinam is 40:1 to 1:

1.

2. The bactericidal composition containing copper octanoate and fluazinam according to claim 1, characterized in that, The weight ratio of copper octoate and fluazinam is 40:1-8:

3.

3. The bactericidal composition containing copper octanoate and fluazinam according to claim 1, characterized in that, The weight ratio of copper octanoate and fluazinam is 20:1-8:

3.

4. The bactericidal composition containing copper octanoate and fluazinam according to claim 1, characterized in that, The weight ratio of copper octoate and fluazinam is 10:1-8:

3.

5. The bactericidal composition containing copper octanoate and fluazinam according to claim 1, characterized in that, The bactericidal composition further comprises at least one agriculturally suitable carrier.

6. The use of the fungicidal composition containing copper octanoate and fluazinam according to claim 1 for controlling plant pathogenic fungal and bacterial diseases, wherein the plant pathogenic fungal and bacterial diseases are bacterial angular leaf spot, powdery mildew, leaf spot, and root rot.

7. The use of the fungicidal composition containing copper octanoate and fluazinam according to claim 1 for protecting seeds, plants, fruits and / or the soil in which plants grow from plant pathogenic fungi and bacterial diseases, wherein the plant pathogenic fungi and bacterial diseases are bacterial angular leaf spot, powdery mildew, leaf spot and root rot.

8. A method for controlling soil-borne diseases, characterized in that, The bactericidal composition containing copper octanoate and fluazinam as described in claim 1 is applied to the soil, wherein the soil-borne diseases are bacterial angular leaf spot, powdery mildew, leaf spot, and root rot.

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