A nucleic acid extraction device that reduces aerosol contamination

Abstract

The application discloses a nucleic acid extraction device capable of reducing aerosol pollution, and relates to the field of biochemical detection instruments.The nucleic acid extraction device comprises a nucleic acid extraction chamber and centrifugal tubes which are detachably installed at two ends of the nucleic acid extraction chamber; a press-to-open and close type sealing layer is fixedly installed on the inner wall of the centrifugal tube close to the tube opening; bosses are arranged at the two ends of the nucleic acid extraction chamber, the bosses are in abutment with the press-to-open and close type sealing layer, and a plurality of narrow slits are formed in the bosses; and a nucleic acid extraction membrane is fixedly installed in the middle of the nucleic acid extraction chamber.The press-to-open and close type sealing layer is arranged at the tube opening of the centrifugal tube, sample injection is achieved through extrusion with the nucleic acid extraction chamber, the exposure time and area of the extracted sample in air can be reduced, the sample in the tube is not prone to leakage from the sealing layer, and the sample outside is also not prone to entering the centrifugal tube through the holes in the sealing layer, so that the problems of aerosol and other cross contamination during pretreatment of nucleic acid samples are effectively solved.

Description

Technical Field

[0001] This invention relates to the field of biochemical detection instrument technology, and in particular to a nucleic acid extraction device that reduces aerosol contamination. Background Technology

[0002] Nucleic acids are a collective term for deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). They are biological macromolecules composed of many nucleotide monomers, whose main function is to express genetic information on proteins, acting as a bridge in the transmission of genetic information. Researchers and medical professionals in the fields of biology and medicine often need to extract nucleic acids from microorganisms for qualitative and quantitative operations such as PCR amplification. Opening the tube after amplification can lead to serious nucleic acid aerosol contamination. The reaction tube contains tens of millions of copies of nucleic acid molecules. When the tube is opened, these molecules diffuse into the air and adhere to various laboratory instruments, causing significant false positives in subsequent nucleic acid testing. Summary of the Invention

[0003] The purpose of this invention is to provide a nucleic acid extraction device that reduces aerosol contamination, thereby solving the problems existing in the prior art.

[0004] To achieve the above objectives, the present invention provides the following solution: The present invention provides a nucleic acid extraction device for reducing aerosol pollution, comprising a nucleic acid extraction chamber and centrifuge tubes detachably installed at both ends of the nucleic acid extraction chamber; a press-to-open and close sealing layer is fixedly installed on the inner wall of the centrifuge tubes near the tube opening; protrusions are provided at both ends of the nucleic acid extraction chamber, the protrusions abut against the press-to-open and close sealing layer, and a plurality of narrow slits are provided on the protrusions; a nucleic acid extraction membrane is fixedly installed in the middle of the nucleic acid extraction chamber.

[0005] Preferably, the press-to-open sealing layer includes a connecting ring and a plurality of sealing flaps. The outer wall of the connecting ring is fixedly connected to the inner wall of the centrifuge tube. The plurality of sealing flaps are all disposed on the inner side of the connecting ring and are arranged along the circumference of the connecting ring. Adjacent sealing flaps are in contact with each other. The sealing flaps are elastic flaps, and the plurality of sealing flaps are integrally formed with the connecting ring.

[0006] Preferably, the outer walls at both ends of the nucleic acid extraction chamber are threaded, and the inner wall of the press-to-open sealing layer at the distance from the centrifuge tube opening is threaded, and the centrifuge tube is threadedly connected to the nucleic acid extraction chamber.

[0007] Preferably, the centrifuge tube and the outer wall of the nucleic acid extraction chamber are provided with anti-slip texture.

[0008] Preferably, both the centrifuge tubes and the nucleic acid extraction chamber are made of polypropylene.

[0009] Preferably, the outer wall of the centrifuge tube is provided with a frosted structure.

[0010] Preferably, the centrifuge tube has graduation lines on its outer wall along the axial direction of the centrifuge tube.

[0011] Preferably, the boss has a conical structure, and the plurality of narrow slits are arranged symmetrically at the center, with the same spacing between adjacent narrow slits.

[0012] The present invention discloses the following technical effects:

[0013] The nucleic acid extraction device for reducing aerosol contamination provided by this invention reduces the exposed area of ​​the sample inside the centrifuge tube by setting a press-to-open and close sealing layer at the centrifuge tube opening. This makes it less likely for the sample inside the centrifuge tube to leak, and also prevents external samples from entering the centrifuge tube through the holes on the vent cap. This effectively solves the problem of cross-contamination between different types of samples when preprocessing a large number of samples of various types. Moreover, the press-to-open and close sealing layer and the nucleic acid extraction chamber together form a highly sealed adsorption environment, which is simple to operate and easy to implement. By setting the centrifuge tube, liquid inlet and outlet are completed by squeezing adjustment, and the centrifuge tube and the nucleic acid extraction chamber are sealed together by threads, which effectively avoids false positive results caused by aerosol contamination. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the nucleic acid extraction device for reducing aerosol pollution according to the present invention;

[0016] Figure 2 This is a schematic diagram of the press-to-open / close sealing layer of the present invention;

[0017] Among them, the nucleic acid extraction chamber-1, centrifuge tube-2, press-to-open and close sealing layer-3, connecting ring-31, sealing membrane flap-32, boss-4, narrow slit-41, nucleic acid extraction membrane-5, and frosted structure-6. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0020] This invention provides a nucleic acid extraction device for reducing aerosol pollution, including a nucleic acid extraction chamber 1 and centrifuge tubes 2 detachably installed at both ends of the nucleic acid extraction chamber 1; a press-to-open and close sealing layer 3 is fixedly installed on the inner wall of the centrifuge tubes 2 near the tube opening; protrusions 4 are provided at both ends of the nucleic acid extraction chamber 1, the protrusions 4 abut against the press-to-open and close sealing layer 3, and a plurality of narrow slits 41 are provided on the protrusions 4; a nucleic acid extraction membrane 5 is fixedly installed in the middle of the nucleic acid extraction chamber 1.

[0021] Furthermore, the press-to-open sealing layer 3 includes a connecting ring 31 and several sealing flaps 32. The outer wall of the connecting ring 31 is fixedly connected to the inner wall of the centrifuge tube 2. Several sealing flaps 32 are all disposed on the inner side of the connecting ring 31, and the several sealing flaps 32 are disposed along the circumference of the connecting ring 31. Adjacent sealing flaps 32 are in contact and fit together. The sealing flaps 32 are elastic flaps, and the several sealing flaps 32 are integrally formed with the connecting ring 31.

[0022] Furthermore, threads are provided on the outer walls at both ends of the nucleic acid extraction chamber 1, and threads are provided on the inner wall of the press-to-open sealing layer 3 at the distance from the opening of the centrifuge tube 2, and the centrifuge tube 2 is threadedly connected to the nucleic acid extraction chamber 1.

[0023] Furthermore, anti-slip textures are provided on the outer walls of centrifuge tube 2 and nucleic acid extraction chamber 1.

[0024] Furthermore, both centrifuge tube 2 and nucleic acid extraction chamber 1 are made of polypropylene.

[0025] Furthermore, the outer wall of the centrifuge tube 2 is provided with a frosted structure 6.

[0026] Furthermore, scale lines are provided on the outer wall of centrifuge tube 2 along the axial direction of centrifuge tube 2.

[0027] Furthermore, the boss 4 has a conical structure, and several narrow slits 41 are arranged in a centrally symmetrical manner, with the same spacing between adjacent narrow slits 41.

[0028] The nucleic acid extraction device for reducing aerosol contamination provided by this invention is suitable for nucleic acid extraction, and its usage method includes the following steps:

[0029] a. Sealing test: First, squeeze the press-to-open seal layer 3 on the centrifuge tube 2. If the press-to-open seal layer 3 can be restored after squeezing, the sealing is good. Second, connect the nucleic acid extraction chamber 1 and the centrifuge tube 2 by thread. If the press-to-open seal layer 3 can be restored after disassembly, the sealing is good.

[0030] b. Install the lower centrifuge tube 2: Take one nucleic acid extraction chamber 1 and one centrifuge tube 2, and install the centrifuge tube 2 to the lower part of the nucleic acid extraction chamber 1;

[0031] c. Install the upper centrifuge tube 2: Take one centrifuge tube 2, squeeze the press-to-open and close sealing layer 3 of the centrifuge tube 2, add the liquid to be extracted, and then invert the centrifuge tube 2. If there is no leakage, install the centrifuge tube 2 onto the upper part of the nucleic acid extraction chamber 1 to complete the installation of the nucleic acid extraction device.

[0032] d. Let stand and wait for the first nucleic acid adsorption;

[0033] e. Multiple nucleic acid adsorption: ① Remove the lower centrifuge tube 2 and replace it with a clean centrifuge tube 2 according to step b; ② Remove the upper centrifuge tube 2, invert the removed lower centrifuge tube 2, and install it on the upper part of the nucleic acid extraction chamber 1 according to step c; ③ Let it stand and wait for the second adsorption; determine the number of adsorption times according to the actual extraction efficiency.

[0034] f. Add to the reaction system: Take centrifuge tube 2 containing the reaction system and assemble it according to step c. Then, perform amplification and specific recognition to complete the detection.

[0035] The nucleic acid extraction device for reducing aerosol contamination provided by the present invention forms a highly sealed adsorption environment by setting up a press-to-open and close sealing layer 3 and a nucleic acid extraction chamber 1. It is simple to operate and easy to implement. By setting up a centrifuge tube 2, the liquid inlet and outlet of the centrifuge tube 2 are completed by squeezing adjustment, and the centrifuge tube 2 and the nucleic acid extraction chamber 1 are sealed by threads, which effectively avoids false positive results caused by aerosol contamination.

[0036] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this invention, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0037] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A nucleic acid extraction device for reducing aerosol contamination, characterized in that, The system includes a nucleic acid extraction chamber (1) and centrifuge tubes (2) detachably installed at both ends of the nucleic acid extraction chamber (1); a press-to-open and close sealing layer (3) is fixedly installed on the inner wall of the centrifuge tubes (2) near the tube opening; protrusions (4) are provided at both ends of the nucleic acid extraction chamber (1), the protrusions (4) abut against the press-to-open and close sealing layer (3), and several narrow slits (41) are provided on the protrusions (4); a nucleic acid extraction membrane (5) is fixedly installed in the middle of the nucleic acid extraction chamber (1); The press-to-open sealing layer (3) includes a connecting ring (31) and a plurality of sealing flaps (32). The outer wall of the connecting ring (31) is fixedly connected to the inner wall of the centrifuge tube (2). The plurality of sealing flaps (32) are all disposed on the inner side of the connecting ring (31) and are disposed along the circumference of the connecting ring (31). Adjacent sealing flaps (32) are in contact with each other. The sealing flaps (32) are elastic flaps and are integrally formed with the connecting ring (31).

2. The nucleic acid extraction device for reducing aerosol pollution according to claim 1, characterized in that, The outer walls at both ends of the nucleic acid extraction chamber (1) are threaded, and the inner wall of the press-to-open sealing layer (3) at the distance from the opening of the centrifuge tube (2) is threaded. The centrifuge tube (2) is threadedly connected to the nucleic acid extraction chamber (1).

3. The nucleic acid extraction device for reducing aerosol pollution according to claim 1, characterized in that, The centrifuge tube (2) and the nucleic acid extraction chamber (1) are both provided with anti-slip textures on their outer walls.

4. The nucleic acid extraction device for reducing aerosol pollution according to claim 1, characterized in that, Both the centrifuge tube (2) and the nucleic acid extraction chamber (1) are made of polypropylene.

5. The nucleic acid extraction device for reducing aerosol pollution according to claim 1, characterized in that, The centrifuge tube (2) has a frosted structure (6) on its outer wall.

6. The nucleic acid extraction device for reducing aerosol pollution according to claim 1, characterized in that, The centrifuge tube (2) has scale lines on its outer wall along the axial direction.

7. The nucleic acid extraction device for reducing aerosol pollution according to claim 1, characterized in that, The boss (4) has a conical structure, and several narrow slits (41) are arranged in a centrally symmetrical manner, with the same spacing between adjacent narrow slits (41).

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