一种嗜碱性粒细胞活化检测方法及其应用
By detecting CD63+ expression in basophils using flow cytometry, combined with SI and absolute activated cell percentage, the accuracy and safety issues of existing allergic disease diagnoses have been resolved, enabling efficient and safe allergen screening and allergy severity assessment.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHANGHAI FIRST PEOPLES HOSPITAL
- Filing Date
- 2022-09-23
- Publication Date
- 2026-07-17
AI Technical Summary
Existing diagnostic methods for allergic diseases suffer from low accuracy, high risk, difficulty in detection, and inability to reflect the patient's internal condition. In particular, in vivo challenge tests may trigger severe allergic reactions, and the detection of allergen-specific IgE is limited.
This invention provides a method for detecting basophil activation. The method involves using flow cytometry to detect the number and proportion of CD63+ cells in CD203c+CD123+CD45+SSlow basophils, and combining the stimulation index (SI) and the percentage of absolutely activated cells. Anti-human CD203c, CD123, CD45, and CD63 antibodies and allergens are used, and negative and positive control groups are set up for in vitro detection.
It achieves highly specific and sensitive diagnosis of allergic diseases, can identify multiple allergens, reduce detection risks, improve diagnostic efficiency, reflect the body's state, and is suitable for predicting the severity of allergies and monitoring treatment efficacy, reducing the probability of misdiagnosis and missed diagnosis.
Smart Images
Figure CN116157672B_ABST