一种可特异性结合PRRSV病毒N蛋白的核酸适配体及其应用

CN116162626BActive Publication Date: 2026-07-17QINGDAO ANIMAL HEALTH NAT ENG TECH RES CENT CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
QINGDAO ANIMAL HEALTH NAT ENG TECH RES CENT CO LTD
Filing Date
2022-10-14
Publication Date
2026-07-17

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Benefits of technology

[0011]本发明筛选获得了可特异性结合PRRSV病毒N蛋白的核酸适配体,所提供的核酸适配体对PRRSV病毒N蛋白的具有极好的特异性和亲和力,为PRRSV病毒的检测和防治提供了重要技术支撑。

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Abstract

本发明提供一种猪繁殖与呼吸综合征病毒PRRSV的N蛋白的核酸适配体及其用途。本发明利用亲和层析柱法,从核酸文库中筛选出了针对PRRSV N蛋白的候选核酸适配体,通过测序比对,选出了重复率较高的适配体,后续通过序列分析、二级结构预测、亲和力和特异性测定等对适配体进行评价,最终获得了两条结合力和特异性极好的PRRSV N蛋白核酸适配体,为后续PRRSV的检测技术的发展和酶联适配体法的建立提供了有利的技术支撑。
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Citation Information

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