A method for preparing rapeseed meal oligopeptide by ultrafine grinding combined with one-step enzymolysis

By combining ultrafine grinding with one-step enzymatic hydrolysis, rapeseed meal oligopeptides can be directly prepared, solving the problem of complex and time-consuming processes in existing technologies, and realizing the efficient preparation of rapeseed meal oligopeptides with antioxidant and ACE inhibitory capabilities.

CN116178487BActive Publication Date: 2026-07-21ACADEMY OF PLANNING & DESIGNING OF THE MINIST OF AGRI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ACADEMY OF PLANNING & DESIGNING OF THE MINIST OF AGRI
Filing Date
2023-02-02
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

Existing methods for preparing oligopeptides from rapeseed meal require first soaking in acid/water or dissolving in alkali to prepare a protein solution, followed by enzymatic hydrolysis. This process is complex and time-consuming, making it difficult to increase the reaction rate.

Method used

Rapeseed meal was physically modified using ultrafine grinding technology. Rapeseed meal oligopeptides were directly prepared by combining ultrafine grinding with one-step enzymatic hydrolysis to reduce particle size and increase specific surface area, thus simplifying the process.

Benefits of technology

This study achieved efficient preparation of rapeseed meal oligopeptides, which possess antioxidant activity and ACE inhibition capabilities, simplifying the process and improving preparation efficiency.

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Abstract

The application provides a method for preparing rapeseed meal oligopeptide by supermicro-pulverization combined with one-step enzymolysis, comprising the following steps: supermicro-pulverization treatment: performing low-temperature supermicro-pulverization treatment on rapeseed cake meal to obtain rapeseed meal supermicro-powder; enzymolysis: performing one-step enzymolysis on the obtained rapeseed meal supermicro-powder to obtain an enzymolysis liquid; refining: performing membrane separation and gel column chromatography separation and purification on the obtained enzymolysis liquid to obtain an active rapeseed meal peptide liquid, and performing drying treatment. The application changes the traditional method for preparing rapeseed meal active peptide, and the cake meal can be directly subjected to enzymolysis treatment after physical modification by micro-pulverization technology, and the preparation process is more simple and easy.
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