Leymus mollis grassland promoting budding fertilizer and its application in rapid recovery of Leymus mollis

Abstract

The present application belongs to the technical field of grassland ecological restoration, and particularly relates to a kind of Leymus chinensis grassland use promotes the application of a kind of fertilizer and its in fast recovery Leymus chinensis in application.The Leymus chinensis grassland use promotes the fertilizer of sprout and includes nitrogen fertilizer, phosphorus fertilizer, silicon fertilizer and rice bran wax paste;in spring snow melting period, the sprout promoting fertilizer is applied, can promote Leymus chinensis tillering, meet the demand of Leymus chinensis to nutrient, promote Leymus chinensis rhizome clonal growth, fast recovery Leymus chinensis in degraded grassland dominant position;And after fertilization, immediately add covering, prevent soil water loss, significantly improve soil moisture, finally reach the effect of improving the density, biomass, crude protein content and its proportion in community of Leymus chinensis in degraded grassland, and two years can realize the recovery of Leymus chinensis population in degraded grassland.

Description

Technical Field

[0001] This invention belongs to the field of grassland ecological restoration technology. More specifically, it relates to a tillering fertilizer for Leymus chinensis grassland and its application in rapidly restoring Leymus chinensis. Background Technology

[0002] The restoration and reconstruction of sheepgrass populations in degraded pastures are of great significance for enhancing the ecological function of sheepgrass grasslands and for the sustainable development of farmers' and herdsmen's production and lives.

[0003] Nutrient supplementation is a crucial measure for rapidly increasing Leymus chinensis yield in grassland ecosystem management. Studies have shown that increased nitrogen content can significantly improve aboveground and belowground biomass and density in Leymus chinensis. For example, Chinese patent application CN103553748A discloses a forage-specific fertilizer mainly composed of urea, potassium sulfate, and superphosphate, which can significantly increase forage yield and improve its nutritional indicators. However, in practical applications, this type of fertilization has not become the primary technical measure for restoring degraded Leymus chinensis grasslands because current fertilization techniques have several drawbacks: firstly, existing fertilizers have low nutrient utilization efficiency and poor application effects; secondly, the nutrient element ratios of existing fertilizers are unreasonable, easily causing nutritional imbalances in Leymus chinensis; and more importantly, existing fertilizers are mostly broadly adaptable, failing to meet the nutrient requirements of Leymus chinensis at various stages of growth and development, making them unsuitable for large-scale application in natural Leymus chinensis grasslands.

[0004] Therefore, there is an urgent need to develop a new type of fertilizer to meet the nutrient requirements of degenerated Leymus chinensis at all stages of its growth and development, promote the clonal growth of Leymus chinensis rhizomes, and quickly restore the dominant position of Leymus chinensis. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the defects and shortcomings of existing nitrogen and phosphorus fertilizers, such as low utilization efficiency and unreasonable nutrient element ratio, and to provide a field fertilizer for promoting tillering of Leymus chinensis that can promote the clonal growth of Leymus chinensis rhizomes and quickly restore the dominant position of Leymus chinensis.

[0006] Another object of the present invention is to provide the application of the aforementioned tillering fertilizer for sheepgrass grasslands in rapidly restoring sheepgrass.

[0007] The above-mentioned objective of this invention is achieved through the following technical solution:

[0008] A tillering fertilizer for sheepgrass grasslands comprises the following components and their weight proportions: 50-150 parts nitrogen fertilizer (based on nitrogen), 40-120 parts phosphorus fertilizer (based on phosphorus pentoxide), 20-40 parts silicon fertilizer (based on silicon dioxide), and 1-3 parts rice bran wax paste.

[0009] Preferably, the grass tillering fertilizer for sheepgrass grassland comprises the following components and their weight proportions: 80-120 parts nitrogen fertilizer (calculated as nitrogen), 60-100 parts phosphorus fertilizer (calculated as phosphorus pentoxide), 20-40 parts silicon fertilizer (calculated as silicon dioxide), and 1-3 parts rice bran wax paste.

[0010] More preferably, the grass tillering fertilizer for sheepgrass includes the following components and their weight parts: 100 parts nitrogen fertilizer, 80 parts phosphorus fertilizer, 30 parts silicon fertilizer, and 2 parts rice bran wax paste.

[0011] Furthermore, the nitrogen fertilizer is urea and diammonium phosphate, wherein the urea has a nitrogen content of ≥46% and the diammonium phosphate has a nitrogen content of ≥18%.

[0012] Furthermore, the phosphorus fertilizer is diammonium phosphate.

[0013] Furthermore, the silicon fertilizer is a water-soluble silicon fertilizer. Silicon fertilizer can improve the resistance of sheepgrass, enhance its photosynthesis, and promote phosphorus absorption.

[0014] Furthermore, the rice bran wax paste is a byproduct separated during the rice bran oil extraction process, which can promote the tillering and stem and leaf growth of Leymus chinensis.

[0015] In addition, the present invention also provides the application of the aforementioned sheepgrass tillering fertilizer in the rapid recovery of sheepgrass, specifically including the following steps:

[0016] S1. Apply sheepgrass tillering fertilizer to uncut degraded hayfields during the spring snowmelt period;

[0017] S2. Cover the area after applying the tillering fertilizer with plant waste, with a coverage amount of 300-600 grams per square meter.

[0018] Leymus chinensis exhibits significant foraging behavior and is highly sensitive to nutrient addition. The Leymus chinensis tillering fertilizer of this invention promotes asexual reproduction of Leymus chinensis rhizomes, increases tillering, and rapidly increases its population density. Furthermore, compared to other grasses, Leymus chinensis prefers a relatively moist environment. Covering the area with plant waste after applying the Leymus chinensis tillering fertilizer significantly increases soil moisture, thereby promoting the growth and spread of the Leymus chinensis population, increasing the density, biomass, crude protein content, and proportion of Leymus chinensis in degraded grasslands, and achieving complete restoration of degraded hayfields within two years.

[0019] Furthermore, the plant waste refers to plant litter or straw. Addressing the problem of reduced surface litter and soil moisture loss caused by years of haymaking in degraded pastures, a method of retaining above-ground vegetation in autumn and adding mulch in spring can, on the one hand, increase the interception of winter snowfall, and on the other hand, reduce surface evaporation by adding mulch in spring, significantly increasing soil moisture content and promoting the germination and growth of spring shoots.

[0020] Furthermore, the application rate of the tillering fertilizer for sheepgrass grassland is 10-30 kg / mu.

[0021] Furthermore, the density of sheepgrass in the degraded hayfield shall not be less than 20 plants per square meter.

[0022] Furthermore, the tillering fertilizer for the sheepgrass grassland is applied 3-5 cm into the soil surface, with a row spacing of 15-25 cm. Fertilization can be performed using a no-till planter, which also helps to loosen the soil and cut the roots.

[0023] Furthermore, prior to step S1, in the autumn, the degraded hayfields are strip-cut at a 1:1 ratio, alternating between uncut and cut degraded hayfields. Full restoration of degraded hayfields can be achieved within two years.

[0024] The present invention has the following beneficial effects:

[0025] 1. The present invention provides a tillering fertilizer for sheepgrass grasslands, comprising nitrogen fertilizer, phosphorus fertilizer, silicon fertilizer, and rice bran wax paste. Applying this fertilizer to sheepgrass grasslands can effectively promote tillering, improve nitrogen and phosphorus nutrient utilization efficiency and soil fertility, meet the nutrient requirements of degraded sheepgrass grasslands, and promote the clonal growth of sheepgrass rhizomes, rapidly restoring the dominant position of sheepgrass. Furthermore, the fertilizer of the present invention is low in cost, ecologically safe, and very suitable for large-scale application. Compared with traditional nitrogen and phosphorus fertilizers, it can significantly increase sheepgrass density and biomass while reducing the amount of nitrogen and phosphorus fertilizer required.

[0026] 2. Most existing technologies involve adding nutrients during the peak growing season, and only a single element is added, resulting in low nutrient utilization, slow recovery, and poor effects. This invention improves nutrient utilization efficiency by adding a grass-based tillering fertilizer during the spring snowmelt period, which corresponds to the spring tillering differentiation period of grass, thus solving the defects of single-element addition and promoting tillering and growth of grass.

[0027] 3. Most existing technologies use reseeding techniques to restore degraded hayfields. However, due to the weak sexual reproduction of Leymus chinensis, low heading rate, low seed setting rate, and low germination rate, it is difficult to restore the Leymus chinensis population in degraded hayfields. This invention makes full use of the characteristic of Leymus chinensis to reproduce asexually through rhizomes, and rapidly restores the Leymus chinensis population by promoting the asexual reproduction of rhizomes.

[0028] 4. Most existing technologies use irrigation to increase soil moisture content, but this is impractical for semi-arid regions. This invention increases the interception of snowfall in winter by preserving above-ground vegetation and reduces soil moisture evaporation in spring by adding cover, thus significantly increasing soil moisture content and creating a moist environment conducive to the differentiation and growth of Leymus chinensis buds in spring. Attached Figure Description

[0029] Figure 1 This is an aerial photograph of the treatment area and the control area in Embodiment 1 of the present invention. The dark-colored area in the figure is the treatment area, and the light-colored area between the two dark-colored stripes is the control area. The two smaller images inside are close-up photos of the treatment area and the control area, showing the recovery effect of Leymus chinensis. Detailed Implementation

[0030] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0031] Unless otherwise specified, all reagents and materials used in the following examples are commercially available.

[0032] Each treatment in the experiment contained 3 replicates, and each replicate (cell) was 20 meters wide and 50 meters long.

[0033] Example 1: A method for rapidly restoring sheepgrass in degraded hayfields

[0034] The experiment consisted of 2 treatments, 3 replicates, and 6 plots in total. The experiment began during the autumn haymaking season, specifically from August 15, 2021 to August 20, 2022.

[0035] (1) Treatment Area: The treatment area was not mowed in August 2021, and the fallen leaves were retained. Treatment was carried out on April 5, 2022. The amount of sheepgrass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) as nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) as phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) as silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the fertilizer was applied to the soil to a depth of 3-5 cm using a no-till planter. After fertilization, the crushed crop straw or withered grass was evenly spread in the treatment area using a fertilizer spreader to cover the surface of the treatment area. The amount of crushed crop straw or withered grass covering per plot was 400 kg.

[0036] (2) Control area: The control area was mowed on August 15, 2021, with a stubble height of 8 cm, and no fertilizer or mulch was added.

[0037] (3) Plant sample collection: For both the treatment and control areas, a 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes and brought back to the laboratory. After drying in an oven at 65℃ for 48 h until constant weight, the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves of Leymus chinensis were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of plant × 6.25.

[0038] See results Figure 1 See Table 1.

[0039] Table 1. Effects of rapid restoration of sheepgrass in degraded hayfields.

[0040]

[0041] Note: *** indicates that the difference between the treatment and control was significant. P The significance level is <0.001.

[0042] As can be seen from the table and figure, the method of rapidly restoring Leymus chinensis according to the present invention can significantly improve the density, biomass, crude protein content and proportion of Leymus chinensis in degraded grasslands, and the Leymus chinensis grows well and can be restored quickly.

[0043] Example 2: Effect of nitrogen fertilizer application rate on sheepgrass recovery

[0044] The study included five nitrogen fertilizer concentration gradient treatments, with three replicates per treatment, for a total of 15 plots. Following the method described in Example 1, sheepgrass restoration operations were performed on degraded hayfields, as detailed below:

[0045] (1) Implementation steps for the first group: The grass was not harvested on August 15, 2021, and the fallen leaves were left on the ground. The treatment was carried out on April 5, 2022. The amount of grass tillering fertilizer used in each plot was equivalent to 5 kg of nitrogen fertilizer (urea and diammonium phosphate) in nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) in phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) in silicon dioxide, and 0.2 kg of rice bran wax paste. After being thoroughly mixed, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or dry grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or dry grass used for covering each plot was 400 kg.

[0046] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0047] Results: Sheepgrass density 242 plants / m² 2 The height of the sheepgrass was 31 cm, the crude protein content was 11.2%, and the biomass was 97 g / m³. 2 Aboveground biomass 236 g / m 2 The proportion of sheepgrass is 41%.

[0048] (2) Implementation steps for the second group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 7.5 kg of nitrogen fertilizer (urea and diammonium phosphate) as nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) as phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) as silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0049] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0050] Results: Sheepgrass density 325 plants / m² 2 The height of the sheepgrass is 37cm, the crude protein content is 12.2%, and the biomass is 139g / m³. 2 Aboveground biomass 273 g / m 2 The proportion of sheepgrass is 51%.

[0051] (3) Implementation steps for the third group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) in nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) in phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) in silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0052] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0053] Results: Sheepgrass density 411 plants / m² 2 The height of the sheepgrass was 39 cm, the crude protein content was 14.6%, and the biomass was 164 g / m³. 2 Aboveground biomass 310 g / m 2 The proportion of sheepgrass is 53%.

[0054] (4) Implementation steps for the fourth group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 12.5 kg of nitrogen fertilizer (urea and diammonium phosphate) as nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) as phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) as silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0055] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0056] Results: Sheepgrass density 406 plants / m² 2 The height of the sheepgrass is 38 cm, the crude protein content is 14.4%, and the biomass is 164 g / m³. 2 Aboveground biomass 300 g / m 2 The proportion of sheepgrass is 55%.

[0057] (5) Implementation steps for the fifth group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 15 kg of nitrogen fertilizer (urea and diammonium phosphate) in nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) in phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) in silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0058] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0059] Results: Sheepgrass density 412 plants / m² 2 The height of the sheepgrass is 37 cm, the crude protein content is 14.5%, and the biomass is 172 g / m². 2 Aboveground biomass 313 g / m 2 The proportion of sheepgrass is 55%.

[0060] The above implementation group's effect data are summarized in Table 2.

[0061] Table 2. Effects of different nitrogen fertilizer application rates on the rapid recovery of Leymus chinensis.

[0062]

[0063] Note: Different letters indicate differences between treatments. P The significance level was <0.05.

[0064] As can be seen from the table, when the nitrogen fertilizer addition amount is 7.5~15 g / m³, 2 At that time, it had a significant promoting effect on the density, height, crude protein content, biomass, aboveground biomass and proportion of Leymus chinensis.

[0065] Example 3: Effect of Phosphorus Fertilizer Addition on Sheepgrass Recovery

[0066] The study included three phosphorus fertilizer concentration gradient treatments, with three replicates for each treatment, for a total of nine plots. Following the method described in Example 1, sheepgrass restoration operations were performed on degraded hayfields, as detailed below:

[0067] (1) Implementation steps for the first group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) in nitrogen, 4 kg of phosphorus fertilizer (diammonium phosphate) in phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) in silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0068] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0069] Results: Sheepgrass density 335 plants / m² 2 The height of the sheepgrass is 34 cm, the crude protein content is 13.8%, and the biomass is 123 g / m³. 2 Aboveground biomass 275 g / m 2 The proportion of sheepgrass is 45%.

[0070] (2) Implementation steps for the second group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) as nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) as phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) as silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0071] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0072] Results: Sheepgrass density 411 plants / m² 2 The height of the sheepgrass is 39cm, the crude protein content is 14.6%, and the biomass is 164g / m³. 2 Aboveground biomass 310g / m 2 The proportion of sheepgrass is 53%.

[0073] (3) Implementation steps for the third group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) in nitrogen, 12 kg of phosphorus fertilizer (diammonium phosphate) in phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) in silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0074] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0075] Results: Sheepgrass density 406 plants / m² 2 The height of the sheepgrass is 38cm, the crude protein content is 14.6%, and the biomass is 169g / m³. 2 Aboveground biomass 312 g / m 2 The proportion of sheepgrass is 54%.

[0076] The above implementation group's effect data are summarized in Table 3.

[0077] Table 3. Effects of different phosphorus fertilizer application rates on the rapid recovery of Leymus chinensis.

[0078]

[0079] Note: Different letters indicate that the difference between treatments reached a significant level of P<0.05.

[0080] As can be seen from the table, when the amount of phosphorus fertilizer added is 8~12 g / m³ 2 When used, it can significantly increase sheepgrass density, sheepgrass biomass, aboveground biomass, and sheepgrass ratio.

[0081] Example 4: Effect of silicon fertilizer addition on the recovery of Leymus chinensis

[0082] The study included three gradient treatments with varying silicon fertilizer application rates, each with three replicates, for a total of nine plots. Following the method described in Example 1, sheepgrass restoration operations were performed on degraded hayfields, as detailed below:

[0083] (1) Implementation steps for the first group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) in nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) in phosphorus pentoxide, 2 kg of silicon fertilizer (water-soluble silicon fertilizer) in silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was then evenly spread in the plots using a fertilizer spreader to cover the surface of the plots. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0084] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0085] Results: Sheepgrass density 345 plants / m² 2 The height of the sheepgrass is 34cm, the crude protein content is 14.4%, and the biomass is 137g / m³. 2 Aboveground biomass 260g / m 2 The proportion of sheepgrass is 53%.

[0086] (2) Implementation steps for the second group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) as nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) as phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) as silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0087] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0088] Results: Sheepgrass density 411 plants / m² 2 The height of the sheepgrass is 39cm, the crude protein content is 14.6%, and the biomass is 164g / m³. 2 Aboveground biomass 310g / m 2 The proportion of sheepgrass is 53%.

[0089] (3) Implementation steps for the third group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) in nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) in phosphorus pentoxide, 4 kg of silicon fertilizer (water-soluble silicon fertilizer) in silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0090] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0091] Results: Sheepgrass density 425 plants / m² 2 The height of the sheepgrass is 39cm, the crude protein content is 14.6%, and the biomass is 171g / m³. 2 Aboveground biomass 317 g / m³ 2 The proportion of sheepgrass is 54%.

[0092] The above implementation group's effect data are summarized in Table 4.

[0093] Table 4. Effects of different silicon fertilizer application rates on the rapid recovery of Leymus chinensis.

[0094]

[0095] Note: Different letters indicate differences between treatments. P The significance level was <0.05.

[0096] As can be seen from the table, when the amount of silicon fertilizer added is 2~4 g / m³ 2 When used, it can significantly increase sheepgrass density, sheepgrass biomass, aboveground biomass, and sheepgrass ratio.

[0097] Example 5: Effect of Rice Bran Wax Paste Addition on Sheepgrass Recovery

[0098] Three gradient treatments with varying amounts of rice bran wax paste were applied, with three replicates for each treatment, totaling nine plots. Following the method in Example 1, sheepgrass restoration operations were performed on degraded hayfields, as detailed below:

[0099] (1) Implementation steps for the first group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) as nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) as phosphorus pentoxide, 2 kg of silicon fertilizer (water-soluble silicon fertilizer) as silicon dioxide, and 0.1 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was then evenly spread in the plots using a fertilizer spreader to cover the surface of the plots. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0100] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0101] Results: Sheepgrass density 386 plants / m² 2 The height of the sheepgrass is 35cm, the crude protein content is 14.4%, and the biomass is 144g / m³. 2 Aboveground biomass 283 g / m 2 The proportion of sheepgrass is 51%.

[0102] (2) Implementation steps for the second group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) as nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) as phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) as silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0103] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0104] Results: Sheepgrass density 411 plants / m² 2 The height of the sheepgrass is 39cm, the crude protein content is 14.6%, and the biomass is 164g / m³. 2 Aboveground biomass 310g / m 2 The proportion of sheepgrass is 53%.

[0105] (3) Implementation steps for the third group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) in nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) in phosphorus pentoxide, 4 kg of silicon fertilizer (water-soluble silicon fertilizer) in silicon dioxide, and 0.3 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0106] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The samples were dried in a 65℃ oven for 48 hours until constant weight, and the aboveground dry weight of each species was recorded. The crude protein content of Leymus chinensis was determined as follows: The crushed stems and leaves were passed through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = Total nitrogen of the plant × 6.25.

[0107] Results: Sheepgrass density 426 plants / m² 2 The height of the sheepgrass is 39cm, the crude protein content is 14.8%, and the biomass is 174g / m³. 2 Aboveground biomass 325g / m 2 The proportion of sheepgrass is 53%.

[0108] The above implementation group's effect data are summarized in Table 5.

[0109] Table 5. Effects of different amounts of rice bran wax paste on the rapid recovery of Leymus chinensis.

[0110]

[0111] Note: Different letters indicate differences between treatments. P The significance level was <0.05.

[0112] As can be seen from the table, when the amount of rice bran wax paste added is 0.1~0.3 g / m 2 At that time, the density, height, crude protein content, biomass, and proportion of Leymus chinensis all increased significantly.

[0113] Example 6: Effect of Mulch Addition Amount on Leymus chinensis Recovery

[0114] The study included three gradient treatments for cover addition, with three replicates for each treatment, totaling nine plots. Referring to the method in Example 1, a sheepgrass restoration operation was performed on degraded hayfields, as detailed below:

[0115] (1) Implementation steps for the first group: The grass was not harvested on August 15, 2021, and the fallen leaves were left on the ground. The treatment was carried out on April 5, 2022. The amount of grass tillering fertilizer used in each plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) in nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) in phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) in silicon dioxide, and 0.2 kg of rice bran wax paste. After being thoroughly mixed, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or dry grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or dry grass used for covering each plot was 200 kg.

[0116] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The aboveground dry weight of each species was recorded after drying in a 65℃ oven for 48 hours to constant weight. The crude protein content of Leymus chinensis was determined as follows: the crushed stems and leaves were sieved through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = total nitrogen of the plant × 6.25. In addition to the above plant sample collection, changes in soil moisture content were also measured: the soil moisture content from 0 to 12 cm was measured using a TDR300 one day before fertilization and one week after the addition of mulch.

[0117] Results: Sheepgrass density 355 plants / m² 2 The height of the sheepgrass is 37cm, the crude protein content is 14.5%, and the biomass is 136g / m³. 2 Aboveground biomass 272 g / m 2The grass content is 50%, and the soil moisture content is 16%.

[0118] (2) Implementation steps for the second group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) as nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) as phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) as silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 400 kg.

[0119] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The aboveground dry weight of each species was recorded after drying in a 65℃ oven for 48 hours to constant weight. The crude protein content of Leymus chinensis was determined as follows: the crushed stems and leaves were sieved through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = total nitrogen of the plant × 6.25. In addition to the above plant sample collection, changes in soil moisture content were also measured: the soil moisture content from 0 to 12 cm was measured using a TDR300 one day before fertilization and one week after the addition of mulch.

[0120] Results: Sheepgrass density 411 plants / m² 2 The height of the sheepgrass is 39cm, the crude protein content is 14.6%, and the biomass is 164g / m³. 2 Aboveground biomass 310g / m 2 The proportion of sheepgrass is 53%, and the soil moisture content is 24%.

[0121] (3) Implementation steps for the third group: The grass was not harvested on August 15, 2021, and the fallen leaves were retained. The grass was treated on April 5, 2022. The amount of grass tillering fertilizer used per plot was equivalent to 10 kg of nitrogen fertilizer (urea and diammonium phosphate) as nitrogen, 8 kg of phosphorus fertilizer (diammonium phosphate) as phosphorus pentoxide, 3 kg of silicon fertilizer (water-soluble silicon fertilizer) as silicon dioxide, and 0.2 kg of rice bran wax paste. After thorough mixing, the mixture was applied to the soil to a depth of 3-5 cm using a no-till planter. The crushed crop straw or withered grass was evenly spread in the plot using a fertilizer spreader to cover the surface of the plot. The amount of crushed crop straw or withered grass used for covering each plot was 600 kg.

[0122] Plant sample collection: A 1 m × 1 m quadrat was set up in each plot. The number of clumps and height of each species were recorded, and the protein content, biomass, and proportion of Leymus chinensis were measured and calculated. Specifically, each species was cut at ground level, packaged in envelopes, and brought back to the laboratory. The aboveground dry weight of each species was recorded after drying in a 65℃ oven for 48 hours to constant weight. The crude protein content of Leymus chinensis was determined as follows: the crushed stems and leaves were sieved through a 0.15 mm (100 mesh) sieve, and the total nitrogen content was determined using an elemental analyzer. Crude protein content = total nitrogen of the plant × 6.25. In addition to the above plant sample collection, changes in soil moisture content were also measured: the soil moisture content from 0 to 12 cm was measured using a TDR300 one day before fertilization and one week after the addition of mulch.

[0123] Results: Sheepgrass density 375 plants / m² 2 The height of the sheepgrass is 38cm, the crude protein content is 14.7%, and the biomass is 157g / m³. 2 Aboveground biomass 286 g / m 2 The proportion of sheepgrass is 55%, and the soil moisture content is 31%.

[0124] The results of the above implementation groups are summarized in Table 6.

[0125] Table 6. Effects of different coverage amounts on the rapid recovery of Leymus chinensis.

[0126]

[0127] Note: Different letters indicate differences between treatments. P The significance level was <0.05.

[0128] As shown in the table, soil moisture content increases with the increase in the amount of plant waste added. However, excessive additives reduce surface sunlight, which is detrimental to plant growth.

[0129] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A method for rapidly restoring sheepgrass, characterized in that, Includes the following steps: S1. Apply sheepgrass tillering fertilizer to uncut degraded hayfields during the spring snowmelt period; S2. Cover the area after applying the tillering fertilizer with plant waste, at a coverage rate of 400 grams per square meter; The sheepgrass tillering fertilizer described in S1 consists of the following components in parts by weight: 100 parts nitrogen fertilizer (calculated as nitrogen), 80 parts phosphorus fertilizer (calculated as phosphorus pentoxide), 30 parts silicon fertilizer (calculated as silicon dioxide), and 2 parts rice bran wax paste. The nitrogen fertilizer is urea and diammonium phosphate, wherein the nitrogen content of urea is ≥46% and the nitrogen content of diammonium phosphate is ≥18%. The phosphorus fertilizer is diammonium phosphate; The silicon fertilizer is a water-soluble silicon fertilizer; The plant waste is crop straw or withered grass; The application rate of the tillering fertilizer for sheepgrass is 10-30 kg / mu.

2. The method according to claim 1, characterized in that, The density of sheepgrass in the degraded hayfield shall not be less than 20 plants per square meter.

3. The method according to claim 1, characterized in that, The tillering fertilizer for the sheepgrass was applied to the soil surface at a depth of 3-5 cm, with a row spacing of 15-25 cm.

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