An apparatus and method for making denaturing polyacrylamide gels

Abstract

The application discloses a denatured polyacrylamide gel manufacturing device and method, relates to the technical field of denatured polyacrylamide gel manufacturing, and can make the short glass plate keep uniform sliding and avoid bubble generation when polyacrylamide gel is manufactured through the setting of a pushing mechanism. The sealing element is arranged between the short glass plate and the long glass plate, glue leakage on four sides of the glass plate in the glue pouring process can be avoided through the setting of the sealing element. The setting of the glue pouring mechanism can guarantee that the glue liquid amount on the long glass plate is kept relatively uniform in the glue pouring process, and the generation of bubbles is avoided. The device does not need manual glue pouring and pushing of the short glass plate sliding in the operation process, the quality of the manufactured polyacrylamide gel is guaranteed, manual operation is avoided, the glue pouring time is shortened, glue failure caused by slow glue pouring and glue solidification is avoided, and the glue manufacturing efficiency is improved.

Description

Technical Field

[0001] This invention relates to the field of modified polyacrylamide gel production technology, and in particular to an apparatus and method for producing modified polyacrylamide gel. Background Technology

[0002] Denaturing polyacrylamide gel electrophoresis (DPGE) is a commonly used electrophoresis technique that uses polyacrylamide as a medium. The gel is denatured by denaturing agents such as urea or formamide, inhibiting base pairing and polymerization. The migration rate of denatured DNA in the gel is almost entirely independent of its base composition and sequence. DPGE is a widely used technique in polymerase chain reaction (PCR) analysis of nucleic acids, primarily for analyzing single-stranded bases, and also used in genetic breeding and gene mapping. Compared to agarose gel electrophoresis, DPGE involves more steps and is more complex, but it offers higher resolution, can separate small nucleic acid fragments differing by only a few or even a single base, and its advantages, such as small sample loading, low diffusion, and ease of observation, make it suitable for SSR molecular marker molecular biology experiments.

[0003] However, during the experiment, when preparing polyacrylamide gels, one hand typically pushes a short glass plate to slide while the other hand uses a syringe to pour the gel. This often leads to several problems, such as: First, uneven pouring speed of the glass plate causes air bubbles, affecting DNA migration during electrophoresis, resulting in bent bands after development, affecting band reading and the analysis of experimental results. If air bubbles are generated during pouring, the glass plate needs to be cleaned again, the solution prepared, and the gel poured again, which is a tedious, time-consuming, and inefficient process. Second, manually pushing the syringe to pour the gel makes it difficult to control the amount poured, and uneven pouring can also cause air bubbles. Third, leakage of gel is likely to occur on all four sides of the glass plate during pouring, resulting in incomplete gel formation and affecting the final gel running effect. Summary of the Invention

[0004] The purpose of this invention is to provide an apparatus and method for preparing modified polyacrylamide gel, so as to solve the problems existing in the prior art.

[0005] To achieve the above objectives, the present invention provides the following solution: The present invention provides an apparatus for producing modified polyacrylamide gel, including a dispensing frame, a groove at the top of the dispensing frame, a short glass plate slidably connected to the dispensing frame via the groove, a slot at one end of the groove, a long glass plate being mounted on the dispensing frame via the slot, the short glass plate corresponding to the long glass plate and positioned above the long glass plate; a pushing mechanism is installed on both sides of the dispensing frame, the pushing mechanism being used to push the short glass plate to slide along the groove; a sealing element is provided between the short glass plate and the long glass plate, the long glass plate and the short glass plate forming a dispensing cavity with one side open through the sealing element; a dispensing mechanism is installed on the top of the short glass plate near the long glass plate, the dispensing mechanism being used to dispense gel into the dispensing cavity.

[0006] Preferably, the dispensing frame includes two parallel support plates, and the slide groove is located on one side of the top of the two support plates facing each other; two connectors are provided between the two support plates, and the two connectors are respectively located at both ends of the support plates. The connectors include a first connecting plate and a second connecting plate, which are respectively fixedly connected to the bottom of the two support plates, and the first connecting plate is located on the side of the second connecting plate closer to the support plate; a threaded hole is opened at the end of the first connecting plate away from the support plate, and an elongated hole is opened on the second connecting plate. A locking bolt is slidably disposed in the elongated hole, and the first connecting plate and the second connecting plate are detachably connected through the locking bolt and the threaded hole; the pushing mechanism is mounted on the support plate.

[0007] Preferably, the top end of the support plate has several grooves, and a fixing clip is provided in the grooves for clamping and fixing the long glass plate and the short glass plate; the bottom end of the support plate has a positioning groove, and the pushing mechanism is installed on the support plate through the positioning groove.

[0008] Preferably, the pushing mechanism includes a base plate, a positioning block fixedly connected to the top of the base plate, the positioning block being correspondingly disposed with the positioning groove; a positioning groove is formed at the top of the positioning block, and the support plate is engaged in the positioning groove; a conveyor belt is installed at the top of the base plate, a slider is fixedly connected to the top of the conveyor belt, a limiting groove is formed at the top of the base plate, the bottom end of the slider is limited and slidably connected in the limiting groove, and the limiting groove is located between the conveyor belt and the positioning block; a pushing rod is fixedly connected to the top of the slider, and the pushing rod is limited and engaged with the end of the short glass plate away from the long glass plate.

[0009] Preferably, the sealing element includes a sealing strip and a gasket. The gasket is arranged along the sliding direction of the short glass plate, and there are two gaskets. The two gaskets are respectively fixedly installed on both sides of the bottom end of the short glass plate, and the bottom end of the gasket slides in engagement with the top end of the long glass plate. The sealing strip is arranged at one end of the long glass plate near the pushing mechanism. The long glass plate and the short glass plate form a potting cavity with one side opening through the gasket and the sealing strip. The sealing strip is used to seal the gaps on both sides and the side edge near the pushing mechanism after the short glass plate and the long glass plate are joined together.

[0010] Preferably, the glue-dispensing mechanism includes a mounting plate fixedly installed on the top of the short glass plate, a liquid storage tank fixedly installed on the top of the mounting plate, a scraper fixedly installed on the mounting plate, the bottom end of the scraper being flush with the bottom end of the short glass plate; a plurality of glue-applying nozzles are installed horizontally on the side of the scraper away from the liquid storage tank, a cavity is formed inside the scraper, and the plurality of glue-applying nozzles are all connected to the cavity; a connecting pipe is connected to the bottom of the liquid storage tank, and the liquid storage tank is connected to the cavity through the connecting pipe; a valve is installed on the connecting pipe.

[0011] Preferably, the adhesive spray nozzle is a flat nozzle.

[0012] Preferably, a vertical plate is fixedly connected to the top of the first connecting plate, a telescopic rod is fixedly installed on the vertical plate, and a glue hole comb is fixedly connected to the end of the telescopic rod. The glue hole comb is correspondingly arranged with the glue filling cavity.

[0013] A method for preparing a modified polyacrylamide gel, specifically including the following steps:

[0014] S1. First, clean the long and short glass plates with detergent, then rinse with ultrapure water, and stand the glass plates upright on the laboratory mat to dry.

[0015] S2. Clean the long and short glass plates three times with 95% alcohol, and wipe them dry with lint-free paper.

[0016] S3. Wipe the long and short glass plates three times with the adhesive adhesion solution, and wipe them dry with lint-free paper. The adhesive adhesion solution is a mixture of 10 mL anhydrous ethanol, 2 μL affinity silane and 5 μL glacial acetic acid. Then wash the short glass plate three times with the glass water repellent, wipe it dry with lint-free paper and let it stand for 10 minutes.

[0017] S4. Place the short glass plate on the dispensing rack, pour the prepared solution into the storage tank, turn on the valve switch on the storage tank and the switch of the push mechanism on both sides of the dispensing rack, the short glass plate along with the gasket moves forward to form a glue chamber and complete the dispensing. After the dispensing is completed, slowly and evenly insert the glue comb into the dispensing chamber to form glue holes.

[0018] S5. After the modified polyacrylamide gel is prepared, push the mechanism and telescopic rod back to their original positions.

[0019] Preferably, in S2 and S3, different gloves should be worn when cleaning long and short glass plates.

[0020] The present invention discloses the following technical effects:

[0021] In the production of polyacrylamide gel, this invention utilizes a pushing mechanism to maintain a uniform sliding speed on the short glass plate, preventing air bubble formation. A sealing element between the short and long glass plates prevents leakage of adhesive from all four sides of the glass plates during the dispensing process. The dispensing mechanism ensures a relatively uniform amount of adhesive sprayed onto the long glass plate during dispensing, further preventing air bubble formation. This invention eliminates the need for manual dispensing and pushing of the short glass plate, ensuring the quality of the produced polyacrylamide gel while avoiding cumbersome manual operations and improving dispensing efficiency. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 This is a top view of the apparatus for preparing the modified polyacrylamide gel of the present invention;

[0024] Figure 2 This is a front view of the glue dispensing frame of the present invention;

[0025] Figure 3 This is a left view of the glue dispensing frame of the present invention;

[0026] Figure 4 This is a top view of the actuation mechanism of the present invention;

[0027] Among them, short glass plate-1, long glass plate-2, support plate-3, slide groove-4, first connecting plate-5, second connecting plate-6, long hole-7, locking bolt-8, groove-9, positioning groove-10, base plate-11, positioning block-12, conveyor belt-13, slider-14, limiting groove-15, push rod-16, seal-17, mounting plate-18, liquid storage tank-19, scraper-20, glue spray nozzle-21, connecting pipe-22, vertical plate-23, telescopic rod-24, glue hole comb-25. Detailed Implementation

[0028] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0030] This invention provides an apparatus for producing modified polyacrylamide gel, including a dispensing frame. A groove 4 is provided at the top of the dispensing frame, and a short glass plate 1 is slidably connected to the dispensing frame via the groove 4. A slot is provided at one end of the groove 4, and a long glass plate 2 is mounted on the dispensing frame via the slot. The short glass plate 1 and the long glass plate 2 are correspondingly arranged, with the short glass plate 1 positioned above the long glass plate 2. Pushing mechanisms are installed on both sides of the dispensing frame to push the short glass plate 1 to slide along the groove 4. A sealing element is provided between the short glass plate 1 and the long glass plate 2, forming a dispensing cavity with one open side. A dispensing mechanism is installed on the top of the short glass plate 1 near the long glass plate 2, and the dispensing mechanism is used to dispense gel into the dispensing cavity.

[0031] In the production of polyacrylamide gel, this invention utilizes a pushing mechanism to maintain the short glass plate 1 at a uniform sliding speed, preventing air bubbles from forming. A sealing element between the short glass plate 1 and the long glass plate 2 prevents glue leakage from all four sides of the glass plates during the glue-pouring process. The glue-pouring mechanism ensures that the amount of glue on the long glass plate 2 remains relatively uniform during the glue-pouring process, further preventing air bubbles. The device eliminates the need for manual glue-pouring and pushing of the short glass plate 1 during operation, ensuring the quality of the produced polyacrylamide gel while avoiding cumbersome manual operations and improving glue-making efficiency.

[0032] Furthermore, to make the dispensing frame suitable for glass plates of different widths, so as to facilitate the production of modified polyacrylamide gels of different specifications, the dispensing frame includes two parallel support plates 3, and a chute 4 is provided on one side of the top of the two support plates 3 facing each other; two connectors are provided between the two support plates 3, and the two connectors are respectively provided at both ends of the support plates 3. The connectors include a first connecting plate 5 and a second connecting plate 6. The first connecting plate 5 and the second connecting plate 6 are respectively fixedly connected to the bottom of the two support plates 3, and the first connecting plate 5 is located on the side of the second connecting plate 6 closer to the support plate 3; a threaded hole is opened at the end of the first connecting plate 5 away from the support plate 3, and an elongated hole 7 is opened on the second connecting plate 6. A locking bolt 8 is slidably provided in the elongated hole 7. The first connecting plate 5 and the second connecting plate 6 are detachably connected through the locking bolt 8 and the threaded hole; a pushing mechanism is installed on the support plate 3.

[0033] Furthermore, in order to facilitate the clamping and fixing of the long glass plate 2 and the short glass plate 1 after glue application, and to facilitate the installation of the pushing mechanism onto the support plate 3, several grooves 9 are provided at the top of the support plate 3, and fixing clips are provided in the grooves 9. The fixing clips are used to clamp and fix the long glass plate 2 and the short glass plate 1. A positioning groove 10 is provided at the bottom of the support plate 3, and the pushing mechanism is installed on the support plate 3 through the positioning groove 10.

[0034] Furthermore, to ensure that the short glass plate 1 can maintain a constant speed forward when it is pushed to slide, the pushing mechanism includes a base plate 11, a positioning block 12 fixedly connected to the top of the base plate 11, and the positioning block 12 is correspondingly set with the positioning groove 10; the top of the positioning block 12 is provided with a positioning groove, and the support plate 3 is engaged in the positioning groove; a conveyor belt 13 is installed on the top of the base plate 11, and a slider 14 is fixedly connected to the top of the conveyor belt 13; a limiting groove 15 is provided on the top of the base plate 11, and the bottom end of the slider 14 is limited and slidably connected in the limiting groove 15, and the limiting groove 15 is located between the conveyor belt 13 and the positioning block 12; a push rod 16 is fixedly connected to the top of the slider 14, and the push rod 16 is limited and engaged with the end of the short glass plate 1 away from the long glass plate 2.

[0035] Furthermore, to prevent glue leakage on all four sides of the glass plate during the glue-filling process, the sealing components include a sealing strip 17 and gaskets. The gaskets are arranged along the sliding direction of the short glass plate 1, and there are two gaskets. The two gaskets are fixedly installed on both sides of the bottom end of the short glass plate 1, and the bottom end of the gaskets slides in cooperation with the top end of the long glass plate 2. The sealing strip 17 is arranged at the end of the long glass plate 2 near the pushing mechanism. The long glass plate 2 and the short glass plate 1 form a glue-filling cavity with one side opening through the gaskets and the sealing strip 17. The sealing strip 17 is used to seal the gaps on both sides and the side edge near the pushing mechanism after the short glass plate 1 and the long glass plate 2 are joined together.

[0036] Furthermore, during the glue application process, to ensure more even application of the glue as the short glass plate 1 moves, the glue application mechanism includes a mounting plate 18 fixedly installed at the top of the short glass plate 1. A liquid storage tank 19 is fixedly installed at the top of the mounting plate 18, and a scraper 20 is fixedly installed on the mounting plate 18. The bottom end of the scraper 20 is flush with the bottom end of the short glass plate 1. Several glue application nozzles 21 are installed horizontally on the side of the scraper 20 away from the liquid storage tank 19. The glue application nozzles 21 are flat nozzles. A cavity is opened inside the scraper 20, and the several glue application nozzles 21 are all connected to the cavity. A connecting pipe 22 is connected to the bottom of the liquid storage tank 19, and the liquid storage tank 19 is connected to the cavity through the connecting pipe 22. A valve is installed on the connecting pipe 22.

[0037] Furthermore, to facilitate the setting of glue holes on the prepared modified polyacrylamide gel, a vertical plate 23 is fixedly connected to the top of the first connecting plate 5, a telescopic rod 24 is fixedly installed on the vertical plate 23, and a glue hole comb 25 is fixedly connected to the end of the telescopic rod 24. The glue hole comb 25 is set correspondingly to the glue filling cavity.

[0038] Furthermore, to facilitate the production of modified polyacrylamide gel, the dispensing frame is configured with one end of the dispensing comb 25 being higher and the other end being lower, with the height difference between the two ends of the dispensing frame controlled between 0.5cm and 1cm.

[0039] The apparatus for preparing modified polyacrylamide gel provided by this invention operates on the following principle:

[0040] First, determine the distance between the two support plates 3 based on the width of the selected glass plate, which is achieved by changing the position of the locking bolt 8 between the first connecting plate 5 and the second connecting plate 6. Then, place the long glass plate 2, which has been processed and fixed with the seal strip 17, into the slot at the top of the support plate 3. Place the short glass plate 1, which has been processed and fixed with the gasket, into the slide groove 4, and make the push rod 16 contact the short glass plate 1 so that after the long glass plate 2 and the short glass plate 1 overlap, a potting cavity with only one opening can be formed with the cooperation of the gasket and the seal strip 17. Then, start the conveyor belt 13 and open the valve on the connecting pipe 22. When the conveyor belt 13 drives the slider 14 to slide on the base plate 11, the slider 14... The push rod 16 on the slide 4 pushes the short glass plate 1 to move along the slide 4. During the movement of the short glass plate 1, the scraper 20 and the liquid storage tank 19 on the short glass plate 1 move together. The adhesive in the liquid storage tank 19 enters the cavity inside the scraper 20 and is sprayed out by several adhesive spray nozzles 21. Since flat nozzles are used, the adhesive is sprayed out in a fan shape. Therefore, by setting multiple adhesive spray nozzles 21, the adhesive can be covered along the width direction of the long glass plate 2. Then, the scraper 20 is used to spread the adhesive evenly on the long glass plate 2, which can avoid the generation of air bubbles when making polyacrylamide gel. After the glue is poured, the telescopic rod 24 is extended to allow the glue comb 25 to be slowly and evenly inserted into the glue pouring cavity to form glue holes. After the glue is made, the long glass plate 2 and the short glass plate 1 are attached together. The short glass plate 1 needs to be removed, and the gel attached to the long glass plate 2 needs to be taken out for staining.

[0041] A method for preparing a modified polyacrylamide gel, specifically including the following steps:

[0042] S1. First, clean the long and short glass plates with detergent, then rinse with ultrapure water, and stand the glass plates upright on the laboratory mat to dry.

[0043] S2. Clean the long and short glass plates three times with 95% alcohol, and wipe them dry with lint-free paper.

[0044] S3. Wipe the long and short glass plates three times with the adhesive adhesion solution, and wipe them dry with lint-free paper. The adhesive adhesion solution is a mixture of 10 mL anhydrous ethanol, 2 μL affinity silane and 5 μL glacial acetic acid. Then wash the short glass plate 1 three times with the glass water repellent, wipe it dry with lint-free paper and let it stand for 10 minutes.

[0045] S4. Place the short glass plate 1 on the glue-filling rack, pour the prepared solution into the storage tank 19, turn on the valve switch on the storage tank 19 and the switch of the push mechanism on the left and right sides of the glue-filling rack, and the short glass plate 1 along with the gasket will move forward to form a glue chamber and complete the glue filling. After the glue filling is completed, slowly and evenly insert the glue hole comb 25 into the glue filling chamber to form glue holes.

[0046] S5. After the modified polyacrylamide gel is prepared, push the mechanism and telescopic rod 24 back to their original positions.

[0047] Because acrylamide and bisacrylamide, used in the production of modified polyacrylamide gel, are highly neurotoxic and easily adsorbed onto the skin, contact with the face, hands, and other skin should be avoided during handling, and gloves must be worn. After gel preparation, any remaining gel solution must be allowed to fully cross-link and solidify before being discarded to prevent environmental pollution.

[0048] Furthermore, to avoid cross-contamination, different gloves must be worn when cleaning long and short glass plates in S2 and S3.

[0049] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this invention, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0050] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. An apparatus for preparing modified polyacrylamide gel, characterized in that, The device includes a glue-filling frame, with a groove (4) at the top. A short glass plate (1) is slidably connected to the glue-filling frame via the groove (4). A slot is provided at one end of the groove (4). A long glass plate (2) is installed on the glue-filling frame via the slot. The short glass plate (1) is correspondingly arranged with the long glass plate (2), and the short glass plate (1) is located above the long glass plate (2). Pushing mechanisms are installed on both sides of the glue-filling frame. The pushing mechanisms are used to push the short glass plate (1) to slide along the groove (4). A sealing element is provided between the short glass plate (1) and the long glass plate (2). The long glass plate (2) and the short glass plate (1) form a glue-filling cavity with one side opening through the sealing element. A glue-filling mechanism is installed on the top of the short glass plate (1) near the side of the long glass plate (2). The glue-filling mechanism is used to fill the glue cavity with glue. The glue-dispensing mechanism includes a mounting plate (18) fixedly installed on the top of the short glass plate (1), a liquid storage tank (19) fixedly installed on the top of the mounting plate (18), a scraper (20) fixedly installed on the mounting plate (18), the bottom end of the scraper (20) being flush with the bottom end of the short glass plate (1); a plurality of glue-applying nozzles (21) are installed horizontally on the side of the scraper (20) away from the liquid storage tank (19), a cavity is opened inside the scraper (20), and the plurality of glue-applying nozzles (21) are all connected to the cavity; a connecting pipe (22) is connected to the bottom of the liquid storage tank (19), and the liquid storage tank (19) is connected to the cavity through the connecting pipe (22), and a valve is installed on the connecting pipe (22).

2. The apparatus for preparing modified polyacrylamide gel according to claim 1, characterized in that, The glue-dispensing frame includes two parallel support plates (3), and the slide groove (4) is located on one side opposite to the top of the two support plates (3). Two connectors are provided between the two support plates (3), and the two connectors are respectively located at both ends of the support plates (3). The connectors include a first connecting plate (5) and a second connecting plate (6). The first connecting plate (5) and the second connecting plate (6) are respectively fixedly connected to the bottom of the two support plates (3), and the first connecting plate (5) is located on the side of the second connecting plate (6) closer to the support plate (3). A threaded hole is provided at the end of the first connecting plate (5) away from the support plate (3), and an elongated hole (7) is provided on the second connecting plate (6). A locking bolt (8) is slidably provided in the elongated hole (7). The first connecting plate (5) and the second connecting plate (6) are detachably connected through the locking bolt (8) and the threaded hole. The pushing mechanism is installed on the support plate (3).

3. The apparatus for preparing modified polyacrylamide gel according to claim 2, characterized in that, The top of the support plate (3) is provided with several grooves (9), and a fixing clip is provided in the groove (9). The fixing clip is used to clamp and fix the long glass plate (2) and the short glass plate (1). The bottom of the support plate (3) is provided with a positioning groove (10), and the pushing mechanism is installed on the support plate (3) through the positioning groove (10).

4. The apparatus for preparing modified polyacrylamide gel according to claim 3, characterized in that, The pushing mechanism includes a base plate (11), a positioning block (12) is fixedly connected to the top of the base plate (11), the positioning block (12) is correspondingly arranged with the positioning groove (10); the top of the positioning block (12) is provided with a positioning groove, and the support plate (3) is snapped into the positioning groove; a conveyor belt (13) is installed on the top of the base plate (11), a slider (14) is fixedly connected to the top of the conveyor belt (13), a limiting groove (15) is provided on the top of the base plate (11), the bottom end of the slider (14) is limited and slidably connected in the limiting groove (15), and the limiting groove (15) is located between the conveyor belt (13) and the positioning block (12); a push rod (16) is fixedly connected to the top of the slider (14), and the push rod (16) is limited and cooperated with the end of the short glass plate (1) away from the long glass plate (2).

5. The apparatus for preparing modified polyacrylamide gel according to claim 1, characterized in that, The sealing element includes a sealing strip (17) and a gasket. The gasket is arranged along the sliding direction of the short glass plate (1). There are two gaskets, and the two gaskets are respectively fixedly installed on both sides of the bottom end of the short glass plate (1). The bottom end of the gasket slides in cooperation with the top end of the long glass plate (2). The sealing strip (17) is arranged at one end of the long glass plate (2) near the pushing mechanism. The long glass plate (2) and the short glass plate (1) are surrounded by the gasket and the sealing strip (17) to form a potting cavity with one side opening. The sealing strip (17) is used to seal the gaps on both sides and the side edge near the pushing mechanism after the short glass plate (1) and the long glass plate (2) are joined together.

6. The apparatus for preparing modified polyacrylamide gel according to claim 1, characterized in that, The adhesive spray nozzle (21) is a flat nozzle.

7. The apparatus for preparing modified polyacrylamide gel according to claim 2, characterized in that, A vertical plate (23) is fixedly connected to the top of the first connecting plate (5), and a telescopic rod (24) is fixedly installed on the vertical plate (23). A glue hole comb (25) is fixedly connected to the end of the telescopic rod (24), and the glue hole comb (25) is correspondingly arranged with the glue filling cavity.

8. A method for preparing a modified polyacrylamide gel, characterized in that, The apparatus for preparing the modified polyacrylamide gel according to any one of claims 1-7 specifically includes the following steps: S1. First, clean the long and short glass plates with detergent, then rinse with ultrapure water, and stand the glass plates upright on the laboratory mat to dry. S2. Clean the long and short glass plates three times with 95% alcohol, and wipe them dry with lint-free paper. S3. Wipe the long and short glass plates three times with the adhesive solution and dry them with lint-free paper. The adhesive solution is a mixture of 10 mL anhydrous ethanol, 2 μL affinity silane and 5 μL glacial acetic acid. Then wash the short glass plate three times with the glass water repellent (1), dry it with lint-free paper and let it stand for 10 minutes. S4. Place the short glass plate (1) on the glue-filling rack, pour the prepared solution into the storage tank (19), turn on the valve switch on the storage tank (19) and the switch of the push mechanism on the left and right sides of the glue-filling rack, and push the short glass plate (1) along with the gasket forward to form a glue chamber and complete the glue filling. After the glue filling is completed, slowly and evenly insert the glue hole comb (25) into the glue filling chamber to form glue holes. S5. After the modified polyacrylamide gel is prepared, push the mechanism and telescopic rod (24) back to their original positions.

9. The method for preparing the modified polyacrylamide gel according to claim 8, characterized in that, In S2 and S3, different gloves must be worn when cleaning long and short glass plates.

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