An experimental animal brain stereotactic partition device and a partitioning method

Abstract

The application relates to the technical field of brain stereoscopic partition, and discloses an experimental animal brain stereotactic partition device and a positioning method, which comprises an experimental animal adapter, a mounting hole is formed in one side of the experimental animal adapter, a positioner is arranged at the bottom end of the one side of the experimental animal adapter, a fixing bolt is threadedly connected with the positioner at the mounting hole, the positioner is fixedly connected with the experimental animal adapter through the fixing bolt, a Y-axis moving assembly is arranged at one side of the positioner, and a z-axis rod is arranged at the top end of the Y-axis moving assembly. The switch assembly is arranged, the cutter clamping piece is pulled down, the limiting block reaches the top end of the switch accommodating groove, when the limiting block is located at the top end of the switch accommodating groove, the switch of the switch protruding block is clamped into the trigger groove of the switch assembly gap sliding groove, the z-axis origin of the lifting rod is changed, and the height is automatically changed.

Description

Technical Field

[0001] This invention relates to the field of brain stereotactic mapping technology, and more specifically to a device for stereotactic mapping of the brain in experimental animals and a method for leveling. Background Technology

[0002] Brain slices are thin slices of brain tissue prepared from animal brain regions, with a thickness of 100-700 μm, that can survive in vitro for a certain period of time. High-quality brain slices are indispensable when studying the characteristics of target neurons and synaptic functions. In order to keep the brain cells in the sample active, the target brain region must be sampled in a short time. Pre-processing the brain into sections is an effective way to save time.

[0003] However, regardless of whether it is live acute sectioning or frozen sectioning, the traditional methods used in the past relied entirely on manual operation when pre-processing brain samples (coronal or sagittal planes) without calibration. This could not guarantee that the cutting surface was flat and that the tissue was perpendicular to the sample holder, often resulting in incomplete and flat brain sections. This was especially true when preparing thicker sections (thickness exceeding 200 μm). To ensure that the extended nerves were not severed and that the complete neural processes and anatomical pathways were preserved for the study of synaptic activity, this invention provides a stereotactic localization and grading device and method for experimental animal brains. Summary of the Invention

[0004] In order to overcome the above-mentioned defects of the prior art, the present invention provides a stereotactic localization and grading device for experimental animal brains and a leveling method to solve the problems existing in the background art.

[0005] This invention provides the following technical solution: a stereotactic localization and grading device and method for laboratory animal brains, comprising a laboratory animal adapter, a mounting hole on one side of the laboratory animal adapter, a positioning device at the bottom of the laboratory animal adapter located at the mounting hole, a fixing bolt threaded onto the positioning device at the mounting hole, the positioning device being fixedly engaged with the laboratory animal adapter through the mounting hole via the fixing bolt, a Y-axis moving component on one side of the positioning device, a Z-axis rod at the top of the Y-axis moving component, a Z-axis Y-axis moving rod in the middle of the Z-axis rod, a lifting rod at the end of the Z-axis Y-axis moving rod near the laboratory animal adapter, a fixing component at the bottom of the lifting rod, a switch component on one side of the bottom of the fixing component, a limit block at the top of the switch component, the switch component being movably engaged within the fixing component via the limit block, and a tool clamping component at the bottom of the switch component.

[0006] Furthermore, the inner side of the tool holder is provided with a tool holding groove, and a tool covering block is provided in the tool holding groove. A pressing block is provided on the side of the tool covering block near the tool holding groove. The tool holding groove and the pressing block are movably connected. One end of a tension spring is fixedly connected to the middle of each pressing block. The other end of each tension spring is fixedly connected to the bottom end of the groove in the tool holding groove. The tool holder securely holds the cutting blade through the tool covering block.

[0007] Furthermore, the experimental animal adapter has height fixing handles on both sides, a horizontal fixing handle in the middle of each height fixing handle, an ear rod on the inner side of each horizontal fixing handle, and a fixing screw on the outer side of the middle of each height fixing handle. The experimental animal adapter has a nose clip height handle on the side near the mounting hole, and a nose clip extension handle on the side of the nose clip height handle near the mounting hole. The nose clip extension handle passes through the nose clip height handle and connects to the nose and mouth clip.

[0008] Furthermore, the nose and mouth clip is movably connected to a water bath, and clamping grooves are provided on both sides of the water bath. A soft sponge is placed at the bottom of the inner wall of the water bath. A fixing clamping block is provided on the side of the water bath near the nose and mouth clip, and the fixing clamping block is adapted to the nose and mouth clip.

[0009] Furthermore, gap grooves are formed on both sides of the switch assembly, and a switch placement groove is formed at the gap groove of the switch assembly. A switch placement groove is formed at the bottom of the switch placement groove of the fixed assembly. A switch protrusion is formed at the gap groove of the switch assembly in the switch placement groove. A switch protrusion is formed at the top of the switch protrusion. When the limiting block is at the bottom of the switch placement groove, a trigger groove for the switch protrusion switch is formed in the intermittent groove of the switch assembly. The switch protrusion will control the lifting rod to change the origin of the z-axis coordinate to the bottom of the cutting blade as the origin of the z-axis coordinate.

[0010] The method of using a stereotactic brain localization and gradation device for experimental animals includes the following: S1. First, weigh the experimental animals. After weighing, use 1% sodium pentobarbital and inject it at a dose of 0.15 mL / 20 g per injection to anesthetize the experimental animals. When injecting, you need to pull back after puncturing to prevent accidentally puncturing the heart of the experimental animal. After the injection is completed, let the experimental animals stand until the drug takes effect. At this time, lift the experimental animals and press the tail and hind legs. If there is no obvious reaction, it means that the experimental animals have been completely anesthetized. At this time, remove the hair on the top of the experimental animal's head. You can use a razor to remove the hair. After roughly removing the hair, align the external auditory canal of the experimental animal's head with one of the horizontal fixing handles (103) of the experimental animal adapter (1) and release the other horizontal fixing handle (103). The two ear rods (104) of the experimental animal adapter (1) are clamped to the head of the experimental animal. At this time, the fixing screw (105) is tightened to completely fix it. At this time, the nose clip (106) is moved back and forth using the nose clip extension handle (108) so that the nose clip (106) clamps the nose part of the experimental animal's head. After confirming that the clamping is tight, the nose clip (106) is clamped and the height is adjusted by the nose clip height handle (107). Then, the straightening degree of the experimental animal is adjusted by adjusting the nose clip extension handle (108) before proceeding to the next step. S2. Disinfect the scalp of the fixed experimental animal and the surgical instruments. After disinfection, use surgical scissors to cut open the scalp of the experimental animal's skull. After cutting, apply eye ointment to the experimental animal. After the experimental animal is tested, start to make the brain stereotactic partitioning device. When making the stereotactic partitioning device, first use surgical scissors to separate the head of the experimental animal from the body. During the separation process, pull the experimental animal's hind legs to straighten the neck of the experimental animal to facilitate decapitation. At this time, ensure that the blood of the experimental animal has been drained. After the blood has been drained, cut off the excess skin and flesh of the experimental animal until it is clean. S3. When performing the sectioning, the skull of the experimental animal needs to be opened first. When opening it, the skull of the experimental animal is first removed from the fixation. At this time, the head of the experimental animal is placed on the soft sponge (402) of the water bath (4). At this time, a small incision is made on both sides of the experimental animal's head at the neck with surgical scissors. After the incision is made, a horizontal cut is made along the middle of the experimental animal's eyeballs with surgical scissors. At this time, the sagittal suture is cut from the middle of the two eyeballs of the experimental animal to facilitate the later prying open of the skull. At this time, the skull is separated from the brain body. After separation, it is placed on the soft sponge (402) of the water bath (4). At this time, the clamping groove (401) of the water bath (4) is fixed with the ear rod (104). At this time, the fixed clamping block (403) and the nose and mouth clamp (106) are fixed to ensure that the experimental animal's brain body on the soft sponge (402) in the water bath (4) is relatively fixed. S4. Due to the need for slicing, the water bath (4) is filled with aSPF artificial cerebrospinal fluid up to two-thirds the height of the soft sponge (402). After the user has returned the Y-axis moving component (3), Z-axis rod (301), Z-axis and Y-axis moving rod (302), and lifting rod (303) to their positions, pulling down the tool holder (3052) will cause the limit block (3051) to reach the top of the switch mounting slot (3042). When the limit block (3051) is at the top of the switch mounting slot (3042), the switch of the switch protrusion (30421) will be engaged in the trigger slot of the gap slide of the switch component (305), thereby causing the Z-axis origin of the lifting rod (303) to move. The tool cover block (3053) is pulled, which causes the tension spring (30531) to be pulled. The inner wall of the cutting blade (306) is then placed into the gap between the clamping block (30532) and the tool clamping groove (30521). After placement, the tool cover block (3053) is released, which causes the tension spring (30531) to rebound. The rebound of the tension spring (30531) clamps the cutting blade (306), thus completing the installation of the cutting blade (306). Since the origin of the lifting rod (303) has been changed, the origin of the changed rod is the bottom of the cutting blade (306). The installation is now complete, and the three-dimensional localization of the experimental animal brain is performed. S5. During the cutting process, the position of the z-axis rod (301) at the Y-axis moving component (3), the position of the z-axis Y-axis moving rod (302) at the z-axis rod (301), and the position of the fixed component (304) at the z-axis Y-axis moving rod (302) are adjusted to adjust the position of the experimental animal brain body that the cutting blade (306) is aligned with. After the position is determined, the lifting rod (303) is moved to drive the fixed component (304) to descend. When the fixed component (304) descends, since the cutting blade (306) is the z-axis origin, the entire device only moves up and down during cutting, so that the overall state of the entire device remains horizontal during cutting, thus ensuring that the cutting blade... When the blade (306) comes into contact with the brain of the experimental animal, the brain is cut by the descent of the blade (306). During the cutting, after the blade (306) has finished cutting, the soft sponge (402) will cause the blade (306) to continue to descend by half the thickness of the soft sponge (402), so that the blade (306) can completely separate the brain of the experimental animal. During the separation, the water bath (4) needs to be finely adjusted so that the water bath (4) is tilted forward or backward. When the blade just touches the surface of the brain, the needle is stopped and the reading is taken so that the height difference between the front and back is within 0.03 mm, which makes it easier to achieve the required slicing in the later stage. After the separation is completed, the blade is returned to its original position. S6. The tool body returns to its original position by pushing the tool cover block (3053) upward, which causes the switch of the switch protrusion block (30421) to leave the trigger slot of the gap slide groove of the switch assembly (305), thereby causing the z-axis origin of the lifting rod (303) to change, and thus the z-axis origin of the lifting rod (303) is changed to the initial state, thus completing the return to its original position. S7. After the fixing block (403) of the water bath (4) is removed from the nose and mouth clamp (106), the clamping groove (401) is then removed. At this point, the experimental animal brain slices on the soft sponge (402) in the water bath (4) can be removed for study.

[0011] The technical effects and advantages of this invention are as follows: 1. This invention, by incorporating a switch assembly, facilitates the downward pulling of the tool clamping component, causing the limiting block to reach the top of the switch mounting slot. When the limiting block is at the top of the switch mounting slot, the switch of the protruding block engages with the trigger slot of the switch assembly gap slide, thereby changing the Z-axis origin of the lifting rod and achieving automatic height adjustment. At this time, the tool cover block is pulled, causing the tension spring to be pulled. The inner wall of the cutting blade is then placed into the gap between the clamping block and the tool clamping slot. After placement, the tool cover block is released, causing the tension spring to rebound, thus clamping the cutting blade. This completes the installation of the cutting blade. Since the Z-axis origin of the lifting rod has been changed, the changed origin is the bottom end of the cutting blade. The installation is now complete, and the experimental animal brain stereotactic zoning is performed.

[0012] 2. This invention, by incorporating a water bath, adjusts the position of the z-axis rod at the Y-axis moving component, the position of the z-axis / Y-axis moving rod at the z-axis rod, and the position of the fixing component at the z-axis / Y-axis moving rod. This adjusts the position of the cutting blade aligned with the experimental animal's brain. Once the position is determined, the lifting rod moves, causing the fixing component to descend. As the fixing component descends, since the cutting blade is at the z-axis origin, the entire device only moves vertically during cutting, maintaining a horizontal overall position. This ensures the cutting blade... When the cutting blade comes into contact with the brain of the experimental animal, it cuts the brain as the blade descends. During the cutting process, after the blade has finished cutting, the soft sponge causes the blade to descend further by half the thickness of the soft sponge, thus completely separating the brain from the experimental animal. During separation, the water bath needs to be finely adjusted to tilt the entire water bath forward or backward so that the needle stops and reads the temperature just after contact with the surface of the brain, ensuring that the height difference between the front and back is within 0.03 mm, which facilitates the subsequent sectioning to meet the requirements. After separation, the blade is returned to its original position. Attached Figure Description

[0013] The present invention will be further described with reference to the accompanying drawings, but the embodiments in the drawings do not constitute any limitation on the present invention. For those skilled in the art, other drawings can be obtained based on the following drawings without creative effort.

[0014] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0015] Figure 2 This is an exploded view of the overall structure of the present invention.

[0016] Figure 3 This is a schematic diagram of the Y-axis moving component structure of the present invention.

[0017] Figure 4 This is a schematic diagram of the switching assembly structure of the present invention.

[0018] Figure 5 This is an exploded view of the switching assembly structure of the present invention.

[0019] Figure 6 This is a schematic diagram of the tool cover block structure of the present invention.

[0020] Figure 7 This is a schematic diagram of the experimental animal adapter structure of the present invention.

[0021] Figure 8 This is an exploded view of the water bath structure of the present invention.

[0022] Figure 9 This is a cross-sectional structural diagram of the fixing component of the present invention.

[0023] Figure 10 This is a schematic cross-sectional view of the fixing component of the present invention.

[0024] Figure 11 This is a schematic diagram of the structure at point A of the present invention.

[0025] The attached figures are labeled as follows: 1. Laboratory animal adapter; 101. Mounting hole; 102. Height fixing handle; 103. Lateral fixing handle; 104. Ear bar; 105. Fixing screw; 106. Nose clip; 107. Nose clip height handle; 108. Nose clip extension handle; 2. Positioning device; 201. Fixing bolt; 3. Y-axis moving assembly; 301. Z-axis rod; 302. Z-axis and Y-axis moving rod; 303. Lifting rod; 304. Fixing assembly. Components; 3041, switch placement slot; 3042, switch mounting slot; 30421, switch protrusion block; 305, switch assembly; 3051, limit block; 3052, tool clamping component; 30521, tool clamping slot; 3053, tool cover block; 30531, tension spring; 30532, clamping block; 306, cutting blade; 4, water bath; 401, clamping slot; 402, soft sponge; 403, fixing clamped block. Detailed Implementation

[0026] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0027] Reference Figure 1-11This invention provides a stereoscopic positioning and grading device and method for laboratory animal brains, including a laboratory animal adapter 1. A mounting hole 101 is provided on one side of the laboratory animal adapter 1. A positioning device 2 is located at the bottom end of the laboratory animal adapter 1 at the mounting hole 101. A fixing bolt 201 is threadedly connected to the positioning device 2 at the mounting hole 101. The positioning device 2 is fixedly engaged with the laboratory animal adapter 1 through the mounting hole 101 via the fixing bolt 201. A Y-axis moving component 3 is provided on one side of the positioning device 2. The top of the device is provided with a z-axis rod 301, the middle of the z-axis rod 301 is provided with a z-axis Y-axis moving rod 302, the end of the z-axis Y-axis moving rod 302 near the experimental animal adapter 1 is provided with a lifting rod 303, the bottom end of the lifting rod 303 is provided with a fixing component 304, one side of the bottom end of the fixing component 304 is provided with a switch component 305, the top of the switch component 305 is provided with a limit block 3051, the switch component 305 is located in the fixing component 304 through the limit block 3051 for movable engagement, and the bottom end of the switch component 305 is provided with a tool clamping component 3052.

[0028] The tool holder 3052 has a tool holding groove 30521 on its inner side, and a tool cover block 3053 is provided in the tool holding groove 30521. A pressing block 30532 is provided on the side of the tool cover block 3053 near the tool holding groove 30521. The tool holding groove 30521 and the pressing block 30532 are movably connected. One end of a tension spring 30531 is fixedly connected to the middle of the pressing block 30532. The other end of the tension spring 30531 is fixedly connected to the bottom end of the groove of the tool holding groove 30521. The tool holder 3052 is fixedly clamped to the cutting blade 306 through the tool cover block 3053.

[0029] The experimental animal adapter 1 has height fixing handles 102 on both sides, a transverse fixing handle 103 in the middle of each height fixing handle 102, an ear rod 104 on the inner side of each transverse fixing handle 103, and a fixing screw 105 on the outer side of the middle of each height fixing handle 102. The experimental animal adapter 1 also has a nose clip height handle 107 on the side near the mounting hole 101, and a nose clip extension handle 108 on the side of the nose clip height handle 107 near the mounting hole 101. The nose clip extension handle 108 passes through... The nose clip height handle 107 is connected to the nose and mouth clip 106.

[0030] The nose and mouth clip 106 is movably connected to a water bath 4. Both sides of the water bath 4 are provided with clamping grooves 401. A soft sponge 402 is placed at the bottom of the inner wall of the water bath 4. A fixing clamping block 403 is provided on the side of the water bath 4 near the nose and mouth clip 106. The fixing clamping block 403 is adapted to the nose and mouth clip 106.

[0031] The switch assembly 305 has gap grooves on both sides. The fixing assembly 304 has a switch placement groove 3041 at the gap groove of the switch assembly 305. The fixing assembly 304 has a switch placement groove 3042 at the bottom of the switch placement groove 3041. The switch placement groove 3042 has a switch protrusion 30421 at the gap groove of the switch assembly 305. The top of the switch protrusion 30421 has a switch. When the limit block 3051 is at the bottom of the switch placement groove 3041, the intermittent groove of the switch assembly 305 has a trigger groove that matches the switch of the switch protrusion 30421. The switch of the switch protrusion 30421 will control the lifting rod 303 to change the origin of the z-axis coordinate to the bottom of the cutting blade 306.

[0032] The method of using a stereotactic brain localization and gradation device for experimental animals includes the following: S1. First, weigh the experimental animals. After weighing, anesthetize the experimental animals by injecting 1% sodium pentobarbital at a dose of 0.15 mL / 20 g per injection. When injecting, a pull-back motion is required after insertion to prevent accidental injection into the heart of the experimental animal. After the injection is completed, let the experimental animal stand until the drug takes effect. At this time, lift the experimental animal and press its tail and hind legs. If there is no obvious reaction, it means that the experimental animal has been thoroughly anesthetized. First, remove the hair on the top of the experimental animal's head. The hair can be removed by using a razor. After roughly removing the hair, align the external auditory canal of the experimental animal's head with one of the horizontal fixing handles 103 of the experimental animal adapter 1, and then release the other horizontal fixing handle 103. The two ear rods 104 of the experimental animal adapter 1 are clamped to the head of the experimental animal. The fixing screws 105 are then tightened for complete fixation. The nose clip 106 is then moved back and forth using the nose clip extension handle 108, thus clamping the nose of the experimental animal. Once the clamping is confirmed to be secure, the height of the nose clip 106 is adjusted using the nose clip height handle 107, and then by adjusting the nose clip extension handle 108. After adjusting the degree of straightening of the experimental animal, proceed to the next step; S2. Disinfect the fixed scalp of the experimental animal and surgical instruments. After disinfection, use surgical scissors to cut open the scalp of the experimental animal. After cutting, apply eye ointment to the experimental animal. After the experimental animal is tested, start to make the brain stereotactic partitioning device. When making the stereotactic partitioning device, first use surgical scissors to separate the head of the experimental animal from the body. During the separation process, pull the experimental animal's hind legs to straighten the neck of the experimental animal to facilitate decapitation. At this time, ensure that the blood of the experimental animal has been drained. After the blood has been drained, cut off the excess skin and flesh of the experimental animal until it is clean. S3. When performing sectioning, the skull of the experimental animal needs to be opened first. Before opening, the skull of the experimental animal is first unfixed. At this time, the head of the experimental animal is placed on the soft sponge 402 in the water bath 4. At this time, a small incision is made on both sides of the experimental animal's head at the neck with surgical scissors. After the incision is made, a horizontal cut is made along the middle of the experimental animal's eyeballs. At this time, the cut is made along the sagittal suture from the middle of the two eyeballs of the experimental animal to facilitate the later prying open of the skull. The skull is then separated from the brain body. After separation, it is placed on the soft sponge 402 in the water bath 4. At this point, the clamping groove 401 of the water bath 4 is fixed with the ear rod 104. At this time, the fixing clamp 403 and the nose and mouth clamp 106 are fixed to ensure that the experimental animal's brain body on the soft sponge 402 in the water bath 4 is relatively fixed. S4. Due to the need for slicing, the water bath 4 is filled with aSPF artificial cerebrospinal fluid up to two-thirds the height of the soft sponge 402. After the user has returned the Y-axis moving component 3, Z-axis rod 301, Z-axis and Y-axis moving rod 302, and lifting rod 303 to their positions, pulling down the tool holder 3052 will cause the limit block 3051 to reach the top of the switch mounting slot 3042. When the limit block 3051 is at the top of the switch mounting slot 3042, the switch of the switch protrusion 30421 will engage with the trigger slot of the gap slide of the switch component 305, thereby causing the Z-axis origin of the lifting rod 303 to change. At this point, the tool cover block 3053 is pulled, which causes the tension spring 30531 to be pulled. The inner wall of the cutting blade 306 is then placed into the gap between the clamping block 30532 and the tool clamping groove 30521. After placement, the tool cover block 3053 is released, which causes the tension spring 30531 to rebound. The rebound of the tension spring 30531 clamps the cutting blade 306, thus completing the installation of the cutting blade 306. Since this has changed the origin of the lifting rod 303, the origin has been changed to the bottom end of the cutting blade 306. The installation is now complete, and the three-dimensional localization of the experimental animal brain is performed. S5. During the cutting process, the position of the z-axis rod 301 at the Y-axis moving component 3, the position of the z-axis / Y-axis moving rod 302 at the z-axis rod 301, and the position of the fixing component 304 at the z-axis / Y-axis moving rod 302 are adjusted to adjust the position of the experimental animal brain body aligned with the cutting blade 306. Once the position is determined, the lifting rod 303 moves, causing the fixing component 304 to descend. When the fixing component 304 descends, since the cutting blade 306 is at the z-axis origin, the entire device only moves up and down during cutting, resulting in the entire device moving more smoothly during cutting. The overall state remains horizontal, so that when the cutting blade 306 contacts the brain of the experimental animal, the brain is cut due to the descent of the cutting blade 306. During the cutting, after the cutting blade 306 has finished cutting, the soft sponge 402 will cause the cutting blade 306 to continue to descend by half the thickness of the soft sponge 402, so that the cutting blade 306 can completely separate the brain of the experimental animal. During separation, the water bath 4 needs to be finely adjusted so that the water bath 4 tilts forward or backward, so that the needle stops and the reading is taken when it just touches the surface of the brain, so that the difference in height between the front and back is within 0.03mm, which facilitates the subsequent slicing to meet the requirements. After the separation is completed, the blade is returned to its original position. S6. The tool body returns to its original position by pushing the tool cover block 3053 upward, which causes the switch of the switch protrusion block 30421 to leave the trigger slot of the gap slide groove of the switch assembly 305, thereby causing the z-axis origin of the lifting rod 303 to change, and the z-axis origin of the lifting rod 303 to the initial state, thus completing the return to its original position. S7. After the fixing block 403 of the water bath 4 is released from the nose and mouth clamp 106, the clamping groove 401 is released. At this point, the experimental animal brain slices on the soft sponge 402 in the water bath 4 can be removed for study.

[0033] Finally, the following points should be noted: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installation", "connection" and "linkage" should be interpreted broadly, and can be mechanical or electrical connection, or internal connection between two components, or direct connection. "Up", "down", "left", "right", etc. are only used to indicate relative positional relationship. When the absolute position of the described object changes, the relative positional relationship may change. Secondly: The accompanying drawings of the embodiments disclosed in this invention only involve the structures involved in the embodiments disclosed in this invention. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of this invention can be combined with each other. In conclusion, the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An experimental animal brain stereotaxic partitioning device comprising an experimental animal adapter (1), characterized in that: The experimental animal adapter (1) has a mounting hole (101) on one side. A positioning device (2) is provided at the bottom of the experimental animal adapter (1) located at the mounting hole (101). A fixing bolt (201) is threadedly connected to the positioning device (2) at the mounting hole (101). The positioning device (2) is fixedly engaged with the experimental animal adapter (1) through the mounting hole (101) of the experimental animal adapter (1) via the fixing bolt (201). A Y-axis moving component (3) is provided on one side of the positioning device (2). A Z-axis rod (301) is provided at the top of the Y-axis moving component (3). A Z-axis Y-axis moving rod (302) is provided in the middle of the Z-axis rod (301). The z-axis and Y-axis moving rod (302) is provided with a lifting rod (303) at one end near the experimental animal adapter (1). The bottom end of the lifting rod (303) is provided with a fixing component (304). The bottom side of the fixing component (304) is provided with a switch component (305). The top of the switch component (305) is provided with a limiting block (3051). The fixing component (304) is provided with a corresponding switch placement slot (3041) and a switch placement slot (3042). The switch component (305) is located in the fixing component (304) and is movably engaged by the limiting block (3051). The bottom end of the switch component (305) is provided with a tool clamping component (3052). The switch assembly (305) has gap grooves on both sides. The fixing assembly (304) has a switch placement groove (3041) at the gap groove of the switch assembly (305). The fixing assembly (304) has a switch placement groove (3042) at the bottom of the switch placement groove (3041). The switch placement groove (3042) has a switch protrusion (30421) at the gap groove of the switch assembly (305). The top of the switch protrusion (30421) is equipped with a switch. When the limiting block (3051) is located at the bottom of the switch placement groove (3041), the gap groove of the switch assembly (305) is equipped with a trigger groove that matches the switch of the switch protrusion (30421). The switch of the switch protrusion (30421) will control the lifting rod (303) to change the origin of the z-axis coordinate to the bottom of the cutting blade (306).

2. The experimental animal brain stereotaxic partition device according to claim 1, wherein: The tool holder (3052) has a tool holding groove (30521) on its inner side. The tool holding groove (30521) has a tool cover block (3053) inside. The tool cover block (3053) has a clamping block (30532) on the side near the tool holding groove (30521). The tool holding groove (30521) and the clamping block (30532) are movably connected. One end of a tension spring (30531) is fixedly connected to the middle of each clamping block (30532). The other end of the tension spring (30531) is fixedly connected to the bottom end of the groove in the tool holding groove (30521). The tool holder (3052) is fixedly clamped to the cutting blade (306) by the tool cover block (3053).

3. The device according to claim 1, wherein: The experimental animal adapter (1) has height fixing handles (102) on both sides, a horizontal fixing handle (103) in the middle of the height fixing handles (102), an ear rod (104) on the inner side of the horizontal fixing handles (103), and a fixing screw (105) on the outer side of the middle of the height fixing handles (102). The experimental animal adapter (1) has a nose clip height handle (107) on the side near the mounting hole (101). The nose clip height handle (107) has a nose clip extension handle (108) on the side near the mounting hole (101). The nose clip extension handle (108) passes through the nose clip height handle (107) and connects to the nose and mouth clip (106).

4. The experimental animal brain stereotaxic partition device according to claim 3, wherein: The nose and mouth clip (106) is movably connected to a water bath (4). The water bath (4) has clamping grooves (401) on both sides. A soft sponge (402) is placed at the bottom of the inner wall of the water bath (4). A fixing clamping block (403) is provided on the side of the water bath (4) near the nose and mouth clip (106). The fixing clamping block (403) is adapted to the nose and mouth clip (106).

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