一种用于斑马鱼幼鱼血红细胞测速的装置及其实现方法

By using collagen layers to immobilize juvenile zebrafish and combining this with fluorescence microscopy and a CCD camera, the problem of easy damage during zebrafish fixation in existing technologies has been solved, enabling non-invasive measurement of red blood cell flow rate and efficient experiments.

CN116269902BActive Publication Date: 2026-07-17HUA XIN WEI YU (SU ZHOU) SHENG WU KE JI YOU XIAN GONG SI
View PDF 4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUA XIN WEI YU (SU ZHOU) SHENG WU KE JI YOU XIAN GONG SI
Filing Date
2023-02-09
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing zebrafish fixation methods are prone to causing fish death and are complex to operate, making it difficult to obtain high-quality images without causing damage.

Method used

Zebrafish juveniles were fixed using a collagen layer, and red blood cell velocimetry was performed using an inverted fluorescence microscope and a CCD camera. The soft structure of the collagen layer enabled non-invasive localization and image acquisition.

Benefits of technology

This method enables non-invasive measurement of red blood cell flow rate, ensuring that zebrafish juveniles can continue to participate in subsequent experiments and improving experimental results.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN116269902B_ABST
    Figure CN116269902B_ABST
Patent Text Reader

Abstract

本申请涉及斑马鱼实验技术领域，公开了一种用于斑马鱼幼鱼血红细胞测速的装置，包括固定组件、成像组件和控制器，所述固定组件包括固定板和盖板，所述固定孔设于所述固定板上，所述盖板盖设于所述固定孔上，所述固定孔用于容纳胶原蛋白层和斑马鱼层，所述成像组件能够采集所述斑马鱼层的图像，所述成像组件与所述控制器电连接。本申请还提供了一种用于斑马鱼幼鱼血红细胞测速的实现方法。本申请能够实现对斑马鱼幼鱼在无损伤条件下测定血红细胞流速，以便于后续采用同一条鱼完成一系列实验的目的，对于探究斑马鱼这类模式动物的生长发育和对药物的反应等领域都有重要意义。
Need to check novelty before this filing date? Find Prior Art