一种电化学发光活性的核酸纳米结构
CN116287117BActive Publication Date: 2026-07-17NANJING UNIV OF SCI & TECH
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NANJING UNIV OF SCI & TECH
- Filing Date
- 2023-02-28
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing DNA nanostructures lack sufficient sensitivity and specificity for biological detection, making it difficult to meet detection needs.
Method used
By preparing electrochemiluminescent nucleic acid nanostructures, a mixture of primers, templates, T4 DNA ligase and phi29 DNA polymerase was used to form a circular template through rolling circle amplification. The template was then reacted with metalloporphyrin and dNTPs under specific conditions to form an electrochemiluminescent nucleic acid nanostructure.
Benefits of technology
This improves the electrochemiluminescence properties and stability of DNA nanostructures, broadening their application areas, particularly in biomarker detection, drug synthesis and delivery, and intracellular RNA imaging.
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Smart Images
Figure CN116287117B_ABST
Abstract
本发明公开了一种电化学发光活性的核酸纳米结构，通过以下制备方法得到：分别将引物、模板、T4DNA连接酶混合，退火环化形成环状模板，再将环状模板、金属卟啉、phi29DNA聚合酶、dNTP混合恒温滚环扩增一段时间后，离心缓冲，最后得到一种电化学发光活性的核酸纳米结构。并对其制备条件进行了优化，最终得到最优的制备条件。本发明以此种具有电化学发光活性的核酸纳米结构的玻碳电极为工作电极，对比了多种金属PPIX辅基的ECL性能，得出含有ZnPPIX的纳米结构电化学发光性能最强。本发明的制备方法，简单可行，所制备的电化学发光活性的核酸纳米结构具有更好的电致化学发光性能。可构建生物传感器，方便地进行原位、现场分析。
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