一种制备长单链DNA的方法
By combining PCR reaction and assisted short-sequence annealing with electrophoretic separation and precipitation concentration techniques, the problems of low efficiency and high cost in the preparation of long single-stranded DNA have been solved, achieving high-yield, low-cost, and easy-to-operate preparation of long single-stranded DNA, which is suitable for routine laboratories.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- JILIN UNIVERSITY
- Filing Date
- 2023-04-19
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies for preparing long single-stranded DNA suffer from low efficiency, high cost, complex operation, and poor versatility. In particular, bioengineering methods require specialized equipment and cumbersome operations, while PCR methods have high requirements for enzyme quality, are costly, and pose a risk of DNA damage.
Double-stranded DNA was accumulated through PCR reaction, and after annealing with auxiliary short sequences and double-stranded DNA, it was separated by agarose gel electrophoresis. Combined with electroelution and precipitation concentration techniques, glycogen or linear polyacrylamide was used to improve the precipitation recovery rate. Finally, the concentration was determined by fluorescence method and characterized by electrophoresis.
It enables efficient, low-cost, and easy-to-operate preparation of long single-stranded DNA with a yield of up to 90%, meeting the needs of routine laboratories, simplifying the operation process, and reducing dependence on equipment and enzymes.
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Figure CN116287123B_ABST