Application of white-cysted Heterobasidion irregulare in relieving low temperature stress of tomato

Abstract

The application relates to application of white cyst rhabdospora in relieving low-temperature stress of tomatoes and belongs to the technical field of microorganisms. The application of white cyst rhabdospora in relieving low-temperature stress of tomatoes; the white cyst rhabdospora is white cyst rhabdospora LL210, and the preservation number is CGMCC No.21057. It is proved through tests that the white cyst rhabdospora LL210 can effectively relieve adverse growth conditions such as slow growth of tomato plants caused by low-temperature stress, and the bacteria have strong adaptability, can grow under weak acid, neutral and weak alkali conditions, can adapt to temperature conditions between 2 DEG C and 50 DEG C, have good compatibility with the environment, and have good development and application prospects. The application adopts fermentation culture liquid of white cyst rhabdospora LL210 to treat and relieve the damage of low temperature to tomatoes, and opens up a new way for guaranteeing safe production of tomatoes in winter.

Description

TECHNICAL FIELD

[0001] The present application relates to the application of white cyst rhabdospora in relieving low temperature stress of tomato, and belongs to the technical field of microorganisms. BACKGROUND

[0002] In recent years, the frequency, intensity and duration of global extreme weather have been increasing. Plants are easily affected by various abiotic stresses during the growth period, among which low temperature is one of the most frequent stress factors. When the growth and development of vegetables are below the suitable temperature, the vegetable crops will suffer from low temperature cold stress, which can lead to the decline of photosynthetic capacity, growth delay, and blocked synthesis metabolism of plants, etc.

[0003] Tomato (Solanum lycopersicum L.) is one of the main protected vegetable crops in China. The existing facility environment control ability in China is weak, and the tomato in the north of China often suffers from low temperature stress in winter and spring. Low temperature affects the synthesis of chlorophyll, nutrient absorption and distribution, and growth and development of tomato plants, leading to physiological disorders such as plant nutrient deficiency, affecting the normal growth and development of tomato, resulting in a substantial decrease in yield and a decrease in quality, thereby causing great economic losses and affecting market supply. Therefore, it is of great practical significance to study the measures to relieve low temperature stress for tomato production.

[0004] At present, the measures to relieve low temperature stress mainly lie in improving the cold resistance or low temperature tolerance of plants, including plant frost hardening, low temperature tolerant variety breeding, exogenous growth regulator regulation, genetic engineering, etc., and there is no report on the use of white cyst rhabdospora to relieve low temperature stress of plants.

[0005] White cyst rhabdospora belongs to non-folding fungi, polyporus family and rhabdospora genus, and is a kind of wood decay fungus, also known as white cyst hole. White cyst rhabdospora contains ligninase, cellulase and laccase and other degradable enzymes, has a fast growth rate and a strong ability to decay wood, and is widely used in industrial papermaking, papermaking wastewater treatment, food and pharmaceutical industry, organic synthesis industry, and pollution treatment and repair of heavy metals. At the same time, the cells of the fungus contain proteins, polysaccharides, adenosine, mannitol and other active substances with high medicinal or health value. Experiments have shown that white cyst rhabdospora can produce antagonistic effect on a variety of pathogenic fungi, and can be used as a biocontrol strain in production, and can promote plant growth and thus increase the biomass of plants. SUMMARY

[0006] The first object of the present application is to provide the application of white cyst rhabdospora in relieving low temperature stress of tomato, and to solve the technical problem that the resistance of tomato to low temperature stress is weak in the prior art.

[0007] The second object of the present application is to provide the application of white cyst rhabdospora in relieving the decrease of photosynthesis of tomato plants caused by low temperature stress.

[0008] The third object of the present invention is to provide a use of white capsule rake tooth fungus in improving chlorophyll fluorescence parameters of tomato plants under low temperature stress.

[0009] The fourth object of the present invention is to provide the use of white capsule rake tooth fungus in promoting the growth of tomato plants.

[0010] A fifth object of the present invention is to provide a use of the white capsule fungus in enhancing the activity of antioxidant enzymes in tomato plants.

[0011] In order to achieve the above object, the technical solution adopted by the present invention is:

[0012] The invention discloses an application of white sac spore fungus in alleviating low temperature stress of tomatoes. The white sac spore fungus is white sac spore fungus LL210, and its preservation number is CGMCC No.21057.

[0013] Specifically, the alleviation of tomato low temperature stress is to improve the resistance of tomatoes to low temperature and reduce the harm of low temperature to plants. White capsule rake tooth fungus LL210 can be used to alleviate the adverse effects of yellowing leaves, weak plants, etc. caused by low temperature stress on tomato plants during the growth period.

[0014] The present invention provides the use of the fungus LL210 for alleviating low-temperature stress in tomatoes. Experiments have shown that the fungus LL210 can maintain the stability of the antioxidant enzyme system in the plant, alleviate the accumulation of reactive oxygen species, and reduce the degree of membrane lipid peroxidation, thereby maintaining cell membrane integrity and enhancing the tomato's resistance to low-temperature stress. Furthermore, the fungus can alleviate the photosynthetic physiological effects of low-temperature stress on tomato plants, restoring the plant's normal vitality and photosynthetic efficiency.

[0015] Preferably, the method comprises the following steps: applying the fermentation culture solution of the white capsule fungus to the rhizosphere of tomatoes to resist the low temperature stress of the tomato plants.

[0016] In actual operation, applying the prepared fermentation culture solution of white capsule fungus to the rhizosphere of tomatoes has the advantages of simple operation and easy control of the specific application amount.

[0017] Further preferably, the fermentation culture of the white sac rake tooth fungus is a white sac rake tooth fungus spore suspension from which the white sac rake tooth fungus hyphae has been removed; the number of spores in the white sac rake tooth fungus spore suspension is 2 to 6×10 8 pieces / mL.

[0018] Preferably, the low temperature stress is 6-10°C during the day and 4-8°C at night.

[0019] The application of white cyst Heterobasidion irregulare in relieving the decrease of photosynthesis of tomato plants caused by low temperature stress, wherein the white cyst Heterobasidion irregulare is white cyst Heterobasidion irregulare LL210 with a preservation number of CGMCC No.21057.

[0020] Tests prove that the white cyst Heterobasidion irregulare LL210 can relieve the photosynthetic physiological influence of tomato plants caused by low temperature stress, and restore the normal vitality and photosynthesis efficiency of the plants.

[0021] The application of white cyst Heterobasidion irregulare in improving chlorophyll fluorescence parameters of tomato plants under low temperature stress, wherein the white cyst Heterobasidion irregulare is white cyst Heterobasidion irregulare LL210 with a preservation number of CGMCC No.21057.

[0022] Tests prove that the white cyst Heterobasidion irregulare LL210 can relieve the influence of low temperature stress on tomato Fv / Fm by improving photosynthetic electron transfer efficiency.

[0023] The application of white cyst Heterobasidion irregulare in promoting the growth of tomato plants, wherein the white cyst Heterobasidion irregulare is white cyst Heterobasidion irregulare LL210 with a preservation number of CGMCC No.21057.

[0024] Tests prove that the white cyst Heterobasidion irregulare LL210 has obvious promoting effect on the growth of tomato plants, and can be applied in promoting the growth of tomato plants.

[0025] Preferably, the fermentation culture solution of white cyst Heterobasidion irregulare is applied to the rhizosphere of tomato plants.

[0026] Preferably, the fermentation culture solution of white cyst Heterobasidion irregulare is a white cyst Heterobasidion irregulare spore suspension from which mycelia of white cyst Heterobasidion irregulare are removed; the number of spores in the white cyst Heterobasidion irregulare spore suspension is 2-6*10 8 / mL.

[0027] Further preferably, the tomato plants are tomato plants under low temperature stress.

[0028] The application of white cyst Heterobasidion irregulare in enhancing the antioxidant enzyme activity in tomato plants, wherein the white cyst Heterobasidion irregulare is white cyst Heterobasidion irregulare LL210 with a preservation number of CGMCC No.21057.

[0029] Tests prove that the white cyst Heterobasidion irregulare LL210 can improve the activity of antioxidant enzymes in tomato plants.

[0030] Preferably, the tomato plants are tomato plants under low temperature stress.

[0031] The application achieves the following beneficial effects:

[0032] 1、The present application discloses white cyst rhabdoclad fungus LL210, which is classified as Irpex lacteus and has a preservation strain number of CGMCC No.21057, has obvious promoting effect on plant growth, can maintain the integrity of cell membrane by maintaining the stable operation of the antioxidant enzyme system in the plant body, relieving the accumulation of active oxygen and reducing the degree of membrane lipid peroxidation, and thus enhancing the resistance of tomatoes to low temperature stress; in addition, the white cyst rhabdoclad fungus can also relieve the photosynthetic physiological influence of tomatoes caused by low temperature stress and restore the normal vitality and photosynthetic efficiency of the plant.

[0033] 2、The white cyst rhabdoclad fungus is a white rot fungus with worldwide distribution, and is non-toxic and non-pathogenic. In the process of co-evolution with host plants, the fungus forms a stable ecological relationship. As a medicinal fungus, the polysaccharide of the white cyst rhabdoclad fungus has good antibacterial and anti-inflammatory effects. The white cyst rhabdoclad fungus LL210 screened from tomato leaves in the present research can effectively relieve the damage of tomato caused by low temperature stress, and the fungus has obvious effect on tomato resistance to low temperature. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 Fig. 1 is a colony morphology of the white cyst rhabdoclad fungus LL210 in Example 1 of the present application;

[0035] Figure 2 Fig. 2 is a fermentation broth morphology of the white cyst rhabdoclad fungus LL210 in Example 1 of the present application;

[0036] Figure 3 Fig. 3 is the growth status of tomato plants under different treatment conditions in Example 2 of the present application;

[0037] Figure 4 Fig. 4 is the influence of different treatment conditions on the antioxidant enzyme activity of the root of tomato plants in Example 3 of the present application. DETAILED DESCRIPTION

[0038] The present application will be further described in combination with specific embodiments, but the protection scope of the present application is not limited to this; if no special description, all kinds of reagents, instruments and the like used in the embodiments are commercially available products.

[0039] Some biological materials, experimental reagents and experimental equipment involved in the following examples and test examples are briefly introduced as follows:

[0040] Biological materials:

[0041] Irpex lacteus LL210 is deposited in the General Microbiology Center of China Culture Collection Administration, with the deposit number CGMCC No. 21057, the deposit date: November 17, 2020, and the deposit address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0042] Example 1 Cultivation of White Capsule Rake Tooth Fungus LL210 and Preparation of Its Spore Suspension

[0043] Mycelium from the LL210 strain of Leucocephalides spp. cultured in a clean bench was removed using an inoculating loop and transferred to PDA (potato dextrose agar) medium. The culture was then incubated in a 26°C incubator for 8 days. Once the mycelium had grown densely in the culture dish, a 9mm diameter cake punch was used to break out the cakes. Six cakes were placed into each Erlenmeyer flask containing 150mL of potato dextrose agar (PD medium). For control treatment, an equal amount of blank PDA cake was inoculated. The culture was shaken at 180 rpm in a 28°C incubator for 4 days. The fermentation broth in the Erlenmeyer flask was used when it became turbid. To facilitate subsequent application and accurately count spores in the fermentation broth, the LL210 fermentation broth was filtered through two layers of regular medical clean gauze to remove the mycelium and obtain a spore suspension. The spore count per mL of the Leucocephalides spp. spore suspension was 3.0×10 spores. 8 indivual.

[0044] The morphological characteristics of the white capsule rake tooth fungus LL210 are as follows:

[0045] The morphological characteristics of the white capsule rake tooth fungus LL210 are as follows Figure 1 As shown by Figure 1 As can be seen in the figure, the colony surface of this strain is white, densely covered with short hairs, with obvious ring marks, thin, wavy and undulating edges, the seed layer under the cap is white or white capsule color, the hyphae are dense, and the edges are neat. It has an aromatic smell.

[0046] The characteristics of the spore suspension of Psoralea corylifolia LL210 are as follows:

[0047] The fermentation broth of the white capsule rake tooth fungus LL210 changes from the initial clear state to a milky white homogenate, which contains a large number of hyphae balls with a diameter of about 3mm-7mm. The morphological characteristics are as follows: Figure 2 The fermentation culture solution was filtered through two layers of clean gauze to obtain a spore suspension. In the subsequent examples of the present invention, the spore suspension of the white sac spore fungus applied to tomato plants was all white sac spore fungus LL210.

[0048] Example 2 Application of White Sac Rake Tooth Fungus LL210 in Alleviating Tomato Low Temperature Stress and Promoting Tomato Plant Growth

[0049] The use of the white sac spore fungus LL210 in this embodiment in alleviating low-temperature stress of tomatoes and promoting the growth of tomato plants specifically relates to the alleviating effect of the white sac spore fungus LL210 on the growth inhibition of tomato seedlings under low-temperature stress.

[0050] After planting and growing, tomatoes were divided into four treatments, with 15 plants in each treatment. These treatments were: control (Control), low temperature treatment (Lts), treatment with a spore suspension of white spores (IL), and treatment with a spore suspension of white spores followed by a low temperature treatment (IL+Lts). The specific operation was as follows: when the tomato plants grew to 4 leaves and 1 heart, 50 mL of a spore suspension of white spores was applied to the rhizosphere of each tomato seedling in the IL and IL+Lts treatments. The spore count per mL of the spore suspension was 3×10 8 The control group (Control) and the low temperature treatment (Lts) were inoculated with the same amount of sterilized PDA culture medium. After 20 days of treatment, the low temperature treatment (Lts) and IL+Lts treatment groups were respectively subjected to low temperature treatment, and the low temperature stress was day: night = 6°C: 4°C. The control group (Control) and the IL treatment were treated at room temperature, and the growth temperature was day: night = 28°C: 20°C. Both low temperature and room temperature treatments were carried out in an artificial climate chamber. After the tomato plants were treated at low temperature for 10 days, the growth of the tomato plants under different treatments was measured. This example was repeated 3 times.

[0051] The results are shown in Table 1. Compared with the control group, the plant height and stem diameter of tomatoes treated with low temperature decreased by 58.52% and 24.32%, respectively, while those treated with the spore suspension of the white spore fungus increased by 28.89% and 45.17%, respectively. Compared with the low temperature treatment group, the plant height and stem diameter of tomatoes in the group treated with the spore suspension of the white spore fungus and then treated with low temperature (IL+Lts) increased by 78.57% and 22.28%, respectively, indicating that the white spore fungus has a significant promoting effect on the growth of tomato plants under low temperature stress. The morphological characteristics of tomato plants are shown in Figure 1. Figure 3 The growth of each experimental group is shown in Table 1.

[0052] Table 1 Effects of different treatments on tomato plant height and stem diameter

[0053] deal with Control Low temperature treatment Lts White capsule rake tooth fungus IL White capsule rake tooth fungus+low temperature IL+Lts Plant height (cm) 27.000±1.581b 11.200±0.570c 34.800±2.588a 26.72±1.581b Stem diameter (mm) 4.910±0.24b 3.716±0.367c 7.128±0.104a 4.726±0.694b

[0054] Note: Different lowercase letters indicate significant differences between the comparative example and the example (P<0.05).

[0055] Example 3 Effects of White Sac Rhacocystis LL210 on Antioxidant Enzyme Activities in Tomato Seedlings Subjected to Low Temperature Stress at the Seedling Stage

[0056] The tomato plants with established growth were divided into four treatments, 15 plants for each. The treatments were: Control, low temperature treatment (Lts), IL treatment, and IL+Lts treatment. The specific operation was as follows: when the tomato plants grew to 4 leaves with one heart, 55 mL of the Ilyssus target tooth fungus spore suspension was applied to the rhizosphere of each tomato seedling for the IL treatment and the IL+Lts treatment, and the spore number of each milliliter of the Ilyssus target tooth fungus spore suspension was 3x10 8 After 20 days of treatment, the low temperature treatment (Lts) and the IL+Lts treatment were subjected to low temperature treatment, and the low temperature stress was day: night = 6°C: 4°C. The control (Control) and the IL treatment were subjected to normal temperature treatment, and the growth temperature was day: night = 28°C: 20°C. The low temperature and normal temperature treatments were carried out in an artificial climate chamber. When the tomato plants were treated under low temperature for 10 days, the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in the roots of the tomato plants in different treatments were measured. This example was repeated 3 times.

[0057] The results are shown in Table 1. Figure 4 As shown in Table 1, under low temperature stress, the activities of peroxidase and catalase in the tomato plants were greatly reduced. The pre-application of the Ilyssus target tooth fungus spore suspension and then the low temperature stress significantly increased the activities of peroxidase and catalase, thereby reducing the oxidative stress and growth inhibition caused by cold damage.

[0058] Example 4: Effect of Ilyssus LL210 on the chlorophyll fluorescence parameter Fv / Fm of tomato seedlings under low temperature stress

[0059] The tomato plants with established growth were divided into four treatments, 15 plants for each. The treatments were: Control, low temperature treatment (Lts), IL treatment, and IL+Lts treatment. The specific operation was as follows: when the tomato plants grew to 4 leaves with one heart, 55 mL of the Ilyssus target tooth fungus spore suspension was applied to the rhizosphere of each tomato seedling for the IL treatment and the IL+Lts treatment, and the spore number of each milliliter of the Ilyssus target tooth fungus spore suspension was 3x10 8Control and Lts were inoculated with equal amount of sterilized PDA medium. After 20 days, Lts and IL+Lts were treated with low temperature stress, which was day: night = 6℃: 4℃. Control and IL were treated with normal temperature, which was day: night = 28℃: 20℃. The low temperature and normal temperature treatments were carried out in artificial climate chamber. When the tomato plants were treated with low temperature for 10 days, the size of PSII maximum photochemical quantum yield (Fv / Fm) of the leaves of the tomato plants in different treatments was determined by chlorophyll fluorescence measurement instrument, and the third leaf was selected for determination. This example was repeated 3 times.

[0060] The results are shown in Table 2. As can be seen from Table 2, compared with the control, the Fv / Fm of the tomato seedlings under low temperature stress decreased, indicating that low temperature stress reduced the photosynthetic electron transport efficiency of the tomato seedlings, the plants were inhibited by light, and the photosynthetic electron transport was hindered. However, after pre-inoculation of the white cyst target tooth fungus spore suspension and then low temperature stress, the Fv / Fm of the tomato had no significant difference from the control, indicating that the white cyst tooth fungus could alleviate the effect of low temperature stress on the Fv / Fm of the tomato by increasing the photosynthetic electron transport efficiency.

[0061] Table 2 Effect of different treatments on PSII maximum photochemical quantum yield of tomato plants

[0062]

[0063] Note: Different lowercase letters represent significant differences (P < 0.05) between the control and the examples.

[0064] Example 5 Effect of white cyst tooth fungus LL210 on photosynthetic characteristics of tomato seedlings under low temperature stress at seedling stage

[0065] The tomato plants with good growth potential were divided into 4 treatments, 15 plants in each treatment. They were: control (Control), low temperature treatment (Lts), white cyst tooth fungus spore suspension treatment (IL), and white cyst tooth fungus spore suspension treatment followed by low temperature treatment (IL+Lts). The specific operation was as follows: when the tomato plants grew to 4 leaves per heart, 55 mL of white cyst target tooth fungus spore suspension was applied to the rhizosphere of each tomato seedling in IL treatment and IL+Lts treatment, and the spore number of each mL of white cyst tooth fungus spore suspension was 3.0 x 10 8The Control and the low temperature treatment (Lts) were inoculated with the same amount of sterilized PDA medium. After 20 days of treatment, the low temperature treatment (Lts) and the IL+Lts treatment group were subjected to low temperature stress, with the low temperature stress being day: night = 6℃: 4℃. The Control and the IL treatment were subjected to normal temperature treatment, with the growth temperature being day: night = 28℃: 20℃. The low temperature and normal temperature treatments were both carried out in an artificial climate chamber. When the tomato plants were subjected to low temperature treatment for 10 days, the stomatal conductance, intercellular carbon dioxide concentration, water use efficiency, transpiration rate, photosynthetic rate, and photosynthetically active radiation of the leaves of the tomato plants in different treatments were measured by using a photosynthesis tester 3051D. This example was repeated three times.

[0066] The results are shown in Table 3. Compared with the Control, the stomatal conductance, intercellular carbon dioxide concentration, water use efficiency, transpiration rate, photosynthetic rate, and photosynthetically active radiation of the tomato plants under low temperature stress were reduced by 74.60%, 11.46%, 76.23%, 80.61%, 48.07%, and 1.03%, respectively, indicating that the photosynthetic capacity of the tomato plants under low temperature stress was reduced. When the white-cyst target dent fungus spore suspension was pre-applied and then subjected to low temperature stress, the photosynthetic rate of the tomato plants had no significant difference from that of the Control, and the transpiration rate of the IL+Lts treatment was 25.34% higher than that of the Control. It can be seen that the white-cyst target dent fungus can alleviate the photosynthetic damage of the tomato plants caused by low temperature stress by adjusting the stomatal opening degree, transpiration rate, and water use efficiency, so as to restore the normal growth of the plants.

[0067] Table 3: Effect of different treatments on photosynthetic parameters of tomato plants

[0068] deal with Control Low temperature treatment Lts White capsule rake tooth fungus IL White capsule rake tooth fungus+low temperature IL+Lts stomatal conductance (mol H2O · m -2 ·s -1 )]]> 0.50±0.03c 0.13±0.02d 0.82±0.03a 0.63±0.06b Intercellular carbon dioxide concentration (ppm) 477.80±1.39c 423.03±2.58d 508.63±3.910a 484.21±1.83b Water utilization rate (mg / g) 0.77±0.07a 0.18±0.03b 0.82±0.02b 0.75±0.09a Transpiration rate (mmol H2O · m -2 · s -1 ) 11.10±0.61b 2.23±0.15c 15.80±0.95a 14.87±1.89a Photosynthetic rate (pmol CO2·m -2 ·s -1 )]]> 1.73±0.12b 0.90±0.04b 4.04±0.82a 1.69±0.20b Photosynthetically active radiation (pmol m -2 ·s -1 )]]> 129.33±2.52c 128.07±3.61c 372.33±18.48a 155.03±6.56b

[0069] In summary, the white-cyst target dent fungus LL210 has obvious promoting effect on the growth of tomato plants. It can maintain the stable operation of the antioxidant enzyme system in the plants, alleviate the accumulation of reactive oxygen species, reduce the degree of membrane lipid peroxidation, maintain the integrity of the cell membrane, and thus enhance the resistance of the tomato plants to low temperature stress. In addition, the white-cyst target dent fungus can also alleviate the photosynthetic physiological impact of the tomato plants caused by low temperature stress, restore the normal viability and photosynthetic efficiency of the plants, and has the effect of alleviating the low temperature stress suffered by the tomato plants.

[0070] Finally, it should be pointed out that the above examples are only used to illustrate the technical solutions of the present application, and not to limit them. Although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that they can still modify the technical solutions described in the foregoing examples, or make equivalent substitutions for some or all of the technical features; and these modifications or substitutions do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. The application of the white capsule rake tooth fungus in alleviating low temperature stress of tomatoes is characterized by: The white capsule rake tooth fungus is white capsule rake tooth fungus LL210, and its preservation number is CGMCC No.21057.

2. The use of the white capsule rake tooth fungus in alleviating low temperature stress of tomatoes according to claim 1, characterized in that: The following steps are involved: The fermented culture of White Sac Fungus was applied to the rhizosphere of tomatoes to resist low temperature stress of tomato plants.

3. The use of the white capsule rake tooth fungus in alleviating low temperature stress of tomatoes according to claim 2, characterized in that: The fermentation culture liquid of the white sac rake tooth fungus is a white sac rake tooth fungus spore suspension from which the white sac rake tooth fungus hyphae has been removed; the number of spores in the white sac rake tooth fungus spore suspension is 2 to 6×10 8 pieces / mL.

4. The use of the white capsule rake tooth fungus according to any one of claims 1 to 3 in alleviating low temperature stress of tomatoes, characterized in that: The low temperature stress is 6-10°C during the day and 4-8°C at night.

5. The use of the fungus Leucospermum erythrorhizium for alleviating the reduction of photosynthesis in tomato plants caused by low temperature stress, characterized in that: The white capsule rake tooth fungus is white capsule rake tooth fungus LL210, and its preservation number is CGMCC No.21057.

6. The use of the fungus Leucospermum erythrorhizium for improving chlorophyll fluorescence parameters of tomato plants subjected to low temperature stress, characterized in that: The white capsule rake tooth fungus is white capsule rake tooth fungus LL210, and its preservation number is CGMCC No.21057.

7. The use of the fungus Leucospermum erythrorhizium for enhancing the activity of antioxidant enzymes in tomato plants at room temperature, characterized in that: The white capsule rake tooth fungus is white capsule rake tooth fungus LL210, and its preservation number is CGMCC No.21057; the antioxidant enzymes are peroxidase, catalase and superoxide dismutase.

8. Use of the fungus Leucospermum leucospermum for enhancing the activity of antioxidant enzymes in tomato plants under low temperature stress, characterized in that: The white cyst is white cyst LL210, with a preservation number of CGMCC No. 21057; and the antioxidant enzymes are peroxidase and catalase.

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