一种UDP-糖基转移酶及其在合成黄酮糖苷衍生物中的应用
By exploring the coupling of UDP-glycosyltransferase UGT73AC11 from Ural licorice with sucrose synthase and constructing a cascade cycle system, the problem of glycosylation modification at the C-7OH site of flavonoids was solved, and the efficient and low-cost synthesis of flavonoid glycoside derivatives was achieved.
CN116334019BActive Publication Date: 2026-07-17BEIJING INST OF TECH
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- BEIJING INST OF TECH
- Filing Date
- 2022-08-05
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing technologies lack UDP-glycosyltransferases that can achieve broad-spectrum glycosylation modification of flavonoid C-7OH sites, and glycosylation modification is costly, making it difficult to achieve large-scale industrial production of flavonoid glycoside derivatives.
Method used
We discovered the UDP-glycosyltransferase UGT73AC11 from Ural licorice and coupled it with sucrose synthase to construct a cascade recycling system, thereby achieving 7-O glycosylation modification of flavonoids and recycling of UDP.
Benefits of technology
This method enables the efficient synthesis of flavonoid glycoside derivatives, reduces the cost of glycosylation modification, provides broad-spectrum catalytic activity, and is suitable for industrial production.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN116334019B_ABST
Abstract
本发明涉及一种来源于乌拉尔甘草的糖基转移酶基因UGT73AC11及其编码的蛋白。本发明通过对乌拉尔甘草基因组数据库的系统分析，从乌拉尔甘草细胞中克隆得到一种来源于UGT73AC11及其编码的蛋白，在大肠杆菌细胞中成功异源表达，并验证其催化功能，同时偶联蔗糖合酶GuSuS1和糖基转移酶UGT73AC11构建UDP循环再生系统，该系统可催化用于合成新甘草苷。本发明解决了能特异性糖基化黄酮C‑7OH位点糖基化修饰且具有广谱性催化功能的糖基转移酶缺乏的问题，为后续合成具有药用前景的新型黄酮糖苷衍生物相关研究提供了一定的依据，同时提供的UDP循环再生体系也极大简便了新甘草苷糖基化修饰过程。
Need to check novelty before this filing date? Find Prior Art