A fluorescent quantitative PCR instrument and detection method
CN116355741BActive Publication Date: 2026-07-21SICHUAN LAI BOYI AUTOMATION TECH CO LTD
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SICHUAN LAI BOYI AUTOMATION TECH CO LTD
- Filing Date
- 2023-04-07
- Publication Date
- 2026-07-21
AI Technical Summary
Technical Problem
Current quantitative PCR instruments have weak detection specificity and low sensitivity, and manual sample loading poses a risk of burns.
Method used
A real-time fluorescence PCR instrument that connects heating and cooling components with detection components uses a sliding component and a motor-driven rack and pinion mechanism to automate the operation of the heating and cooling components. Combined with the covering of the hot cap and detection, it realizes the cyclic heating, cooling and detection of samples.
Benefits of technology
It improves the specificity and sensitivity of detection, avoids the risk of burns caused by manual sample loading, simplifies the instrument structure, and reduces maintenance costs.
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Smart Images
Figure CN116355741B_ABST
Abstract
The application discloses a fluorescent quantitative PCR instrument and a detection method, and particularly relates to the technical field of PCR instruments; wherein the PCR instrument comprises a shell, a heating and refrigerating assembly, a detection assembly and an upper cover assembly are arranged in the shell, the heating and refrigerating assembly is connected with the detection assembly, and the upper cover assembly is used for being overlaid on the heating and refrigerating assembly; an opening is arranged on the front end surface of the shell, a moving mounting plate is arranged at the position corresponding to the opening in the shell, and the moving mounting plate is used for reciprocating motion at the opening through a sliding assembly; the heating and refrigerating assembly and the detection assembly are both arranged on the same moving mounting plate, and the upper cover assembly is arranged on the top of the shell; the upper cover assembly comprises a thermal cover; the application aims at solving the problems that the specificity of the PCR instrument in the prior art is weak and the sensitivity of analysis is low when detecting the sample to be detected, and simultaneously solving the detection influence caused by manual sample loading, and automatically heating the heating and refrigerating assembly to prevent the scalding risk existing in manual sample loading.
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