源于棉花二倍体野生种异常棉的抗旱相关的SSR序列及其应用

By using distant hybridization and marker-assisted selection, a specific SSR molecular marker fragment on chromosome 8 of the abnormal cotton was replaced into upland cotton, which solved the problem of reduced drought resistance in cotton breeding, improved the drought resistance of cotton and accelerated the breeding speed, and provided fine localization and analysis of drought resistance genes.

CN116355946BActive Publication Date: 2026-07-17JIANGSU ACAD OF AGRI SCI +1

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU ACAD OF AGRI SCI
Filing Date
2023-03-01
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The genetic diversity of cotton decreases during cultivation, leading to a weakening of its drought resistance. Existing technologies make it difficult to effectively utilize the drought-resistant gene resources of wild cotton species.

Method used

By using distant hybridization and marker-assisted selection, specific SSR molecular marker fragments on chromosome 8 of abnormal cotton are replaced into upland cotton to develop cotton varieties or lines with improved drought resistance. Specific DNA fragments are used to replace the original chromosome fragments of the recipient parent, and screening and identification are carried out in combination with SSR molecular markers and primer pairs.

Benefits of technology

It enabled early, rapid, and accurate improvement of cotton drought resistance, provided fine mapping and functional analysis of drought-resistant genes, promoted the selection efficiency and breeding speed of drought-resistant cotton breeding, and obtained cotton lines with drought-resistant introgression chromosome segments.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明公开了源于棉花二倍体野生种异常棉的抗旱相关的SSR序列及其应用。本发明提供了特定DNA片段或其相关生物材料在培育抗旱棉花品种或品系或提高棉花的抗旱性中的应用;所述特定DNA片段为位于异常棉8号染色体上至少含有自SSR分子标记NAU2914(SEQ ID No.1)起至SSR分子标记JAAS6420(SEQ ID No.7)止的片段;所述相关生物材料为含有所述特定DNA片段的载体、表达盒、重组菌或转基因细胞系。本发明所提供的染色体片段在抗旱棉花育种中具有重要应用价值,同时本发明开发的SSR分子标记为培育能应用于生产的抗旱棉花品种提供分子基础。
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