Fe3O4@SiO2-Au磁性纳米复合材料构建的电化学生物传感器及其构建方法与应用
An electrochemical biosensor was constructed by preparing Fe3O4@SiO2-Au magnetic nanocomposite materials, which solved the problems of low sensitivity and long detection time in the detection of the novel coronavirus, and achieved rapid, low-cost, and highly sensitive detection.
CN116359300BActive Publication Date: 2026-07-17HEFEI UNIV OF TECH
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HEFEI UNIV OF TECH
- Filing Date
- 2023-02-15
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing electrochemical biosensors suffer from low sensitivity, high cost, and long detection time in COVID-19 detection.
Method used
Fe3O4@SiO2-Au magnetic nanocomposites were prepared, and ACE2 was immobilized through a large specific surface area and abundant surface groups to construct a highly sensitive electrochemical biorecognition device. Label-free electrochemical impedance spectroscopy was used to detect the spike protein of the novel coronavirus.
Benefits of technology
It achieves a detection limit as low as femtograms, rapidly detecting the SARS-CoV-2 spike protein within 5 minutes, and exhibits good anti-interference, stability, and repeatability.
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Abstract
本发明公开一种Fe3O4@SiO2‑Au磁性纳米复合材料构建的电化学生物传感器及其构建方法与应用。电化学生物传感器通过合成Fe3O4@SiO2‑Au复合材料并作为电化学修饰材料检测新冠病毒的刺突蛋白，考虑了溶剂热法合成的均匀分散的Fe3O4的纳米颗粒，改进法包覆SiO2，金纳米颗粒的单独合成后掺杂，最后合成了Fe3O4@SiO2‑Au磁性纳米复合材料。本发明以提高新冠病毒检测的灵敏度，降低成本，检测时间缩短等为目标，制备出Fe3O4@SiO2‑Au纳米复合材料，以其大的比表面积和表面丰富的基团最大化固定ACE2。基于ACE2和新冠病毒刺突蛋白紧密结合，ACE2生物功能化的磁性材料进行构建高灵敏的电化学生物识别装置对新冠病毒刺突蛋白进行无标记电化学阻抗法测定，其检测限低至皮克级别。
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