一株香叶基香叶醇合成酿酒酵母工程菌的构建及应用
By strengthening the mevalonate pathway, introducing gerany pyrophosphate synthase and NADH-dependent HMGR gene into Saccharomyces cerevisiae, and weakening the sterol synthesis pathway, the problem of insufficient IPP and DMAPP supply in Saccharomyces cerevisiae was solved, and the efficient synthesis of gerany geraniol was achieved, with a yield of 5.57 g/L, which has industrialization potential.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- JIANGNAN UNIV
- Filing Date
- 2023-04-21
- Publication Date
- 2026-07-17
AI Technical Summary
In the existing technology, the synthesis of geranyl by Saccharomyces cerevisiae suffers from insufficient supply of IPP and DMAPP and low synthesis efficiency of geranyl pyrophosphate, which limits its efficient synthesis.
By enhancing the mevalonate pathway in Saccharomyces cerevisiae, introducing gerany-gerany pyrophosphate synthase and NADH-dependent HMGR genes, and weakening the sterol synthesis pathway, specific measures included integrating the expression of specific genes and adjusting promoters, optimizing gene copy number and fermentation conditions.
It significantly improved the synthesis capacity of geraniol, with a yield of 5.57 g/L after 120 hours of fermentation, which is the highest record to date and has promising prospects for industrial application.
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Figure CN116376734B_ABST