一种天然活性胶原蛋白快速定性定量检测试剂盒、检测方法及应用

By employing specific dye staining and porous membrane filtration technology, this method solves the problems of time-consuming qualitative and quantitative collagen detection and inability to distinguish similar substances in existing technologies, achieving rapid and accurate collagen detection suitable for food, cosmetics, and medical aesthetic products.

CN116380889BActive Publication Date: 2026-07-17DONGGUAN NIUNIU NEW MATERIAL TECH CO LTD +1

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DONGGUAN NIUNIU NEW MATERIAL TECH CO LTD
Filing Date
2023-05-24
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies make it difficult to quickly, easily, and accurately perform qualitative and quantitative detection of natural active collagen, especially in distinguishing collagen from its analogues such as gelatin and hydrolyzed collagen.

Method used

Collagen is stained with an acidic solution of a specific dye to generate a flocculent precipitate, which is then filtered through a porous membrane or filter cartridge. Qualitative and quantitative analysis is achieved by combining visual observation and spectrometer detection.

Benefits of technology

It enables rapid qualitative and quantitative detection of natural active collagen, with short processing time and high specificity. It can effectively distinguish gelatin and hydrolyzed collagen and similar substances, and is suitable for real-time online detection of food, cosmetics and medical aesthetic products.

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Abstract

本发明涉及天然活性胶原蛋白检测技术领域,具体涉及一种天然活性胶原蛋白快速定性定量检测试剂盒、检测方法及应用,该检测试剂盒包括至少一个试剂管,试剂管包括用于盛放检测液的管体、用于将管体内的溶液排出的挤出部以及用于将管体内的天然活性胶原蛋白沉淀进行截留的过滤部;该检测方法包括如下步骤:取出试剂管;吸取待测样品,滴加至试剂管中;摇匀;目视观察沉淀生成状态;将试剂管内的溶液排出,将截留的天然活性胶原蛋白沉淀进行结果比对。本发明的试剂盒可通过肉眼观察待测样本加入后是否有沉淀生成可有效鉴定活性胶原蛋白;实现定量检测时,胶原蛋白类似物(明胶、水解胶原蛋白)对结果无干扰;15分钟内可完成检测,便于实时在线检测。
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