Compositions and uses thereof

Abstract

The present application relates to the technical field of biological new material, particularly relates to a composition and application thereof.The present application provides a novel carboxyl magnetic microparticle sealing liquid, and main components include: CB buffer, methoxypolyethylene glycol amine, taurine.The sealing liquid is used for enhancing hydrophilicity of carboxyl magnetic microparticles after coating, and the sealing + cleaning measures of CB buffer, methoxypolyethylene glycol amine and taurine are used in combination, which can effectively seal the residual active groups after the completion of carboxyl magnetic microparticle coating, and simultaneously enhance the water and layer of magnetic microparticles and the electric properties, improve the storage agglutination problem of carboxyl magnetic microparticles after coating protein, and can reduce the non-specific adsorption of magnetic microparticle components in the chemiluminescence detection process.

Description

Technical Field

[0001] This invention relates to the field of new biomaterials technology, and particularly to compositions and their applications. Background Technology

[0002] Magnetic microparticles, as the primary solid-phase carrier in chemiluminescence immunoassay, offer advantages such as superparamagnetism and high specific surface area. As a polymer material prepared from polystyrene, magnetic microparticles inherently possess hydrophobic properties. This hydrophobicity presents both advantages and disadvantages in their applications. The advantage lies in the fact that hydrophobicity itself facilitates protein adsorption, ensuring sufficient protein adsorption during coating and guaranteeing adequate sensitivity of the detection product. The disadvantage is that hydrophobicity makes magnetic microparticles prone to aggregation during storage. This aggregation makes it difficult to mix uniformly during detection, affecting test results and customer perception. Insufficient sealing after coating can also lead to significant hydrophobic interactions, causing the adsorption of enzyme conjugates during detection, resulting in a severely elevated background and affecting the detection gradient.

[0003] In conventional magnetic microparticle coating processes, blocking solutions containing proteins (such as BSA) are typically used for blocking and preservation. However, due to the relatively large size of proteins, the active groups generated during activation may not be completely blocked by the proteins, leading to enzyme adsorption during the detection process. Furthermore, the hydrophobic structure of the coating material can also cause enzyme adsorption.

[0004] In existing literature or patents, no dual-function cleaning and sealing solution has been found that combines these three compounds to reduce non-specific adsorption and magnetic particle aggregation. Therefore, providing such a dual-function solution is of great significance. Summary of the Invention

[0005] In view of this, the composition and its application provided by the present invention can reduce the background luminescence value of magnetic microparticles and reduce the agglomeration particles generated during the storage of magnetic microparticles.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0007] This invention provides a composition comprising an alkaline buffer solution, a PEG-NH2 compound, and taurine;

[0008] The alkaline buffer solution includes CB buffer solution;

[0009] The PEG-NH2 compounds include one or more of methoxy polyethylene glycolamine, polyethylene glycolamine, or Y-type polyethylene glycolamine.

[0010] In some specific embodiments of the present invention, the above composition includes:

[0011] The concentration of the alkaline buffer solution is 0.05 mol / L; and / or

[0012] The concentration of the PEG-NH2 compound is 10–50 mg / mL.

[0013] In some specific embodiments of the present invention, the above composition includes:

[0014] The CB buffer solution comprises 1.59 g / L sodium carbonate and 2.93 g / L sodium bicarbonate; and / or

[0015] The concentration of the methoxy polyethylene glycolamine is 10–50 mg / mL; and / or

[0016] The concentration of taurine is 5–10 mg / mL.

[0017] In some specific embodiments of the present invention, the above composition includes:

[0018] The molecular weight of the methoxy polyethylene glycol amine is 2000 to 20000; and / or

[0019] The polyethylene glycolamine has a molecular weight of 5000; and / or

[0020] The molecular weight of the Y-type polyethylene glycolamine is 15,000.

[0021] The present invention also provides for the use of the above composition in any of the following:

[0022] (I) Reduce the hydrophobic effect on the surface of magnetic particles; and / or

[0023] (II) Reduce the non-specific adsorption of magnetic particles; and / or

[0024] (III) Reduce the aggregation of magnetic particles during storage; and / or

[0025] (IV) Reduce the background luminescence value in chemiluminescent immunoassay; and / or

[0026] (V) Enhancing the luminescence gradient in chemiluminescent immunoassay; and / or

[0027] (VI) Enclosure of magnetic particles; and / or

[0028] (VII) Cleaning of magnetic particles; and / or

[0029] (VIII) Preparation of magnetic microparticle sealing solution; and / or

[0030] (IX) Preparation of magnetic particle cleaning solution; and / or

[0031] (X) Preparation of a dual-function treatment solution for cleaning and sealing magnetic microparticles.

[0032] The present invention provides a treatment liquid for magnetic microparticles, comprising the above-described composition, and acceptable excipients or additives;

[0033] The treatment fluid includes a sealing fluid, a cleaning fluid, or a dual-function cleaning and sealing treatment fluid.

[0034] The present invention also provides a method for preparing the above-mentioned treatment solution, comprising the following steps:

[0035] Step (1): Mix 1.59g sodium carbonate, 2.93g sodium bicarbonate and 1000mL water to obtain CB buffer;

[0036] Step (2): Take the CB buffer solution from step (1) and mix it with 10g of methoxy polyethylene glycolamine and 5g of taurine to obtain the treatment solution.

[0037] The present invention also provides a method for sealing magnetic microparticles, comprising: mixing a sealing reagent with magnetic microparticles, reacting for 30 to 120 minutes, and then ending the sealing process;

[0038] The sealing reagent includes the above-described composition, the above-described treatment solution, or the treatment solution prepared by the above-described preparation method.

[0039] The present invention also provides a method for cleaning magnetic microparticles, comprising: mixing a cleaning reagent with magnetic microparticles, reacting for 10 minutes, removing the supernatant, repeating the process 3 times, and ending the cleaning process;

[0040] The cleaning reagent includes the above-described composition, the above-described treatment solution, or the treatment solution prepared by the above-described preparation method.

[0041] The composition of the present invention has the following effects:

[0042] 1. Experiments show that the background of magnetic microparticles treated with the novel sealing liquid is reduced by more than 50% compared with conventional processes, resulting in a doubling of the gradient, which helps to improve specificity;

[0043] 2. Experiments show that after conventional process and novel sealing liquid coating of magnetic microparticles are stored at 4°C for 6 months and then mixed for 1 hour using a homogenizer, the conventional process still has a strong particle feel after mixing, while the magnetic microparticles treated with novel sealing liquid have no obvious particle feel. That is, the anti-agglomeration performance of magnetic microparticles is significantly improved and they are less likely to form particles. Attached Figure Description

[0044] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0045] Figure 1 Example 1 shows a comparison of the storage and agglomeration effects of the two processes;

[0046] Figure 2 Example 2 shows a comparison of the agglomeration effect when methoxy polyethylene glycol amine or other polyethylene glycol derivatives are added;

[0047] Figure 3 Example 3 shows a comparison of the agglomeration effects when different taurine contents are added;

[0048] Figure 4 Example 4 shows a comparison of the agglomeration effects when different taurine substitutes are added. Detailed Implementation

[0049] This invention discloses the composition and its application. Those skilled in the art can refer to the content herein and appropriately modify the process parameters to achieve the desired results. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in this invention. The methods and applications of this invention have been described through preferred embodiments. Those skilled in the art can clearly modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit, and scope of this invention to realize and apply the technology of this invention.

[0050] This invention utilizes a combined technique of CB buffer, methoxy polyethylene glycol amine, and taurine to prepare a novel protein-free, dual-function treatment solution for magnetic microparticles. The principle is as follows: The amino groups of methoxy polyethylene glycol amine, a PEG-NH2 derivative, react with unreacted activated groups on the surface of the magnetic microparticles, forming a hydrated layer and reducing non-specific adsorption. The amino groups on taurine react with these unreacted activated groups, giving the coated magnetic microparticles a strong negative charge and enhancing their dispersion performance. The alkaline condition of the CB buffer causes some loosely adsorbed active material to detach, further reducing non-specific adsorption. The combined use of these three components helps strengthen the hydrated layer of the magnetic microparticles, increasing hydrophilicity, reducing non-specific adsorption, and minimizing aggregation caused by hydrophobic interactions. The simultaneous action of these three mechanisms effectively mitigates the performance problems of magnetic microparticles caused by hydrophobic non-specific adsorption.

[0051] The novel carboxyl-based magnetic microparticle blocking solution is used as follows: After washing the carboxyl-based magnetic microparticles with PBS buffer and removing the supernatant, EDC / NHS coupling agent is added to convert the carboxyl groups on the magnetic microparticles into active ester groups. After washing with MES buffer, active material is added to couple with the active ester groups on the magnetic microparticles. After coupling, this novel blocking solution is used for blocking and washing. After blocking, a protein-containing protective solution is used for preservation.

[0052] This invention provides a novel blocking liquid that enhances the hydrophilicity of magnetic microparticles and reduces nonspecific adsorption. The novel carboxyl-based magnetic microparticle blocking liquid comprises the following components:

[0053] a. CB buffer;

[0054] b. Methoxylated polyethylene glycol amine;

[0055] c. Taurine

[0056] Preparation method: Dissolve a certain amount of methoxy polyethylene glycolamine and taurine in CB buffer solution and mix until completely dissolved.

[0057] This invention also provides a novel carboxyl magnetic microparticle blocking solution, the method of use of which is as follows:

[0058] 1) Prepare a CB buffer solution of a certain molar concentration by dissolving a certain amount of methoxy polyethylene glycolamine and taurine in the CB buffer solution to prepare this novel carboxyl magnetic microparticle blocking solution.

[0059] 2) After washing the carboxyl magnetic microparticles with PBS buffer and removing the supernatant, EDC / NHS coupling agent was added to convert the carboxyl groups on the magnetic microparticles into active ester groups. After washing with MES buffer, active material was added to couple the active ester groups on the magnetic microparticles.

[0060] 3) After coupling is completed, use this new blocking solution for blocking. After blocking, wash and store with a protein-containing preservation solution.

[0061] The novel carboxylated magnetic microparticle blocking solution mentioned above comprises CB buffer, methoxy polyethylene glycolamine, and taurine; the recommended concentrations are: CB buffer 0.05 mol / L; methoxy polyethylene glycolamine (molecular weight 5000) concentration 10-50 mg / mL; taurine concentration 5-10 mg / mL.

[0062] The magnetic microparticles mentioned in the above usage method are carboxyl magnetic microparticles with a carboxyl content of 10-60 μmol / g and a particle size of 0.8-1.4 μm;

[0063] The coated living material is a mouse monoclonal antibody.

[0064] The coating working solution described in the above usage method is selected from PBS buffer, morpholine ethanesulfonic acid buffer, and CB buffer;

[0065] The coupling reaction time described in the above method of use is 1 to 3 hours.

[0066] The sealing time using this novel sealing liquid in the above coating method is 30 min to 2 h;

[0067] The preservation solution described in the above coating method comprises PBS buffer, bovine serum albumin (BSA), Tween-20, and Proclin 300 in a volume ratio of 1000 mL:(9.8–10.2) g:(0.98–1.02) mL:(0.98–1.02) mL.

[0068] The concentration of the EDC solution in the above coating method is 10 mg / mL to 20 mg / mL, and the concentration of the NHS solution is 10 mg / mL to 20 mg / mL.

[0069] The above coating method also includes washing the amino magnetic nanoparticles with PBS buffer 4 to 6 times and then removing the supernatant, with each washing time being 5 to 10 minutes.

[0070] Wash with preservation solution 4 to 6 times to remove supernatant, with each wash lasting 5 to 10 minutes.

[0071] The present invention also provides a kit comprising carboxyl magnetic microparticles prepared by the above preparation method.

[0072] This invention provides a novel magnetic microparticle sealing liquid, including the method of using this novel sealing liquid in the magnetic microparticle coating process described above.

[0073] In this invention, the carboxyl magnetic nanoparticles are washed 4-6 times with PBS buffer and the supernatant is removed, each time for 5-10 minutes; then, a novel sealing solution is used to wash 4-6 times and the supernatant is removed, each time for 5-10 minutes. In a specific embodiment, the carboxyl magnetic nanoparticles are washed 4 times with PBS buffer and the supernatant is removed, each time for 5 minutes; then, a novel sealing solution is used to wash 4 times and the supernatant is removed.

[0074] This invention uses CB buffer, methoxy polyethylene glycol amine, and taurine to prepare a novel protein-free blocking solution, which has the dual functions of blocking and cleaning magnetic microparticles. It effectively reduces the hydrophobic forces on the surface of magnetic microparticles, helps to reduce non-specific adsorption, and alleviates the aggregation of magnetic microparticles during storage.

[0075] Unless otherwise specified, the raw materials, reagents, consumables and instruments involved in this invention are all commercially available products and can be purchased from the market.

[0076] The present invention will be further illustrated below with reference to the embodiments:

[0077] Example 1

[0078] The process of coating magnetic microparticle carcinoembryonic antigen 153 antibody, hereinafter referred to as CA153:

[0079] 1. Prepare a novel blocking solution: Prepare 1L of 0.05mol / L CB buffer (1.59g sodium carbonate, 2.93g sodium bicarbonate, 1000mL purified water); weigh 10g of methoxy polyethylene glycol amine (molecular weight 5000); weigh 5g of taurine, and add them to the CB buffer in sequence, mixing until completely dissolved.

[0080] 2. Take Merck carboxymagnetic microparticles (100 mg / mL, 30 μL), wash 4 times (300 μL of 0.1 mol / L PBS × 4 times, 5 min each time) to remove the supernatant, add 100 μL of activator solution (10 mg / mL each of EDC and NHS, dissolved in MES buffer), mix well again and place on a shaker to shake for 1 h;

[0081] After removing the supernatant by magnetic attraction, add 300 μL of MES buffer and wash twice, then remove the supernatant again. Add 90 μL of coating MES buffer and 10 μL of CA153 antibody (5 mg / mL) to coat the live material. After mixing with a rapid mixer, place on a shaker and shake for 2 hours, then remove the supernatant.

[0082] Wash 4 times with the prepared new blocking solution (300μL×4 times, 10min each time), and remove the supernatant after the blocking and washing is completed.

[0083] The protein was preserved at a constant volume using a standard protein-containing preservation solution, which included PBS buffer, bovine serum albumin (BSA), Tween-20, and Proclin 300 in a volume ratio of 1000 mL: 10 g: 1 mL: 1 mL.

[0084] This invention uses the Antu Bio A2000Plus detection instrument to compare the coating potency of the magnetic microparticle kits prepared in the examples, and uses the instrument's mixer to compare the magnetic microparticle storage and agglomeration. Two kits were coated using both conventional coating processes and the process described in this invention (after cleaning with the blocking solution). One kit was used for coating potency comparison, and the other was stored at 4°C for 6 months and then mixed with the instrument's mixer for 1 hour to compare the magnetic microparticle agglomeration status.

[0085] Table 1: Comparison of coating valence and background of the two processes

[0086]

[0087] (Note: b0 is negative serum; c1 and c2 are the medium and high value calibration points prepared using b0 as the matrix, respectively.)

[0088] As shown in Table 1, the coating titer of the magnetic microparticles CA153 prepared using the present invention in the examples was slightly lower than that of magnetic microparticles prepared by conventional processes. This may be because the increased hydrophilicity of the magnetic microparticles after washing with the novel blocking solution reduced the adsorption of enzyme conjugates and also washed away poorly coated antibodies, resulting in a slight decrease in luminescence value. However, the background of the magnetic microparticles treated with the novel blocking solution was reduced by more than 50% compared to the conventional process, resulting in a doubling of the gradient, which helps to improve specificity. This is beneficial to the performance improvement of the magnetic microparticle kit.

[0089] Depend on Figure 1 As can be seen, after the magnetic microparticles were coated with the two processes and stored at 4°C for 6 months, they were mixed with a homogenizer for 1 hour using a testing instrument. The conventional process still had a strong particle feel after mixing, while the magnetic microparticles treated with the new sealing liquid had no obvious particle feel. That is, the magnetic microparticles have significantly improved anti-agglomeration performance during storage and are less likely to form particles.

[0090] Using the novel sealing liquid of this invention, the background of magnetic particles is significantly reduced and the gradient is increased, and the storage state of magnetic particles is also improved. Therefore, this invention is more applicable.

[0091] Example 2

[0092] Taking coated magnetic microparticles CA153 as an example, this article details the application scope of methoxylated polyethylene glycol amines and other polyethylene glycol derivatives:

[0093] 1. Prepare a novel blocking solution: Prepare 1L of 0.05mol / L CB buffer; weigh 10g of methoxy polyethylene glycol amine (different molecular weights are shown in Table 2); weigh 5g of taurine, and add them to the CB buffer in sequence, mixing until completely dissolved.

[0094] Table 2

[0095]

[0096] The preparation process and performance testing scheme of magnetic microparticles are shown in Example 1.

[0097] The valence comparisons are shown in Table 3:

[0098] Table 3

[0099]

[0100]

[0101] Its storage and agglomeration effect is, for example, Figure 2 As shown.

[0102] From Table 3 and Figure 2It is known that the molecular weight of methoxy polyethylene glycolamine has little effect on the gradient and background, but storage aggregation reappears when the molecular weight reaches 20,000. Therefore, the recommended molecular weight of methoxy polyethylene glycolamine is 2,000 to 10,000.

[0103] 2. Preparation of novel blocking solution: Prepare 0.05mol / L CB buffer, 1L; 5000 methoxy polyethylene glycol amine (different amounts are shown in Table 4); weigh 5g of taurine, add them to the CB buffer in sequence, and mix until completely dissolved.

[0104] Table 4

[0105] Sealing liquid 1 Sealing liquid 2 Sealing liquid 3 Sealing liquid 4 Sealing liquid 5 0 mg / mL 1mg / mL 10mg / mL 20mg / mL 50mg / mL

[0106] The preparation process and performance testing scheme of magnetic microparticles are shown in Example 1.

[0107] The valence comparisons are shown in Table 5:

[0108] Table 5

[0109]

[0110] As can be seen from the above examples, the applicable dosage range of methoxy polyethylene glycolamine is 10-50 mg / mL. Lower dosage will increase the background level, while higher dosage will have no significant effect.

[0111] 3. Prepare a novel blocking solution: Prepare 1L of 0.05mol / L CB buffer; weigh 10g of PEG-NH2 compound (as shown in Table 6); weigh 5g of taurine, and add them to the CB buffer in sequence, mixing until completely dissolved.

[0112] Table 6

[0113]

[0114] The preparation process and performance testing scheme of magnetic microparticles are shown in Example 1.

[0115] The valence comparisons are shown in Table 7:

[0116] Table 7

[0117]

[0118] As can be seen from the above examples, PEG-NH2 compounds can all reduce the background and improve the gradient, but PEG is less effective.

[0119] Example 3

[0120] Taking coated magnetic microparticles CA153 as an example, this article details the application scope of taurine and other similar compounds:

[0121] 1. Prepare a novel blocking solution: Prepare 1L of 0.05mol / L CB buffer; weigh 10g of methoxy polyethylene glycol amine (molecular weight 5000); add taurine as shown in Table 8 to the CB buffer and mix until completely dissolved.

[0122] Table 8

[0123] Sealing liquid 1 Sealing liquid 2 Sealing liquid 3 Sealing liquid 4 Taurine - 0 mg / mL Taurine - 5 mg / mL Taurine - 10 mg / mL Taurine - 50 mg / mL

[0124] The preparation process and performance testing scheme of magnetic microparticles are shown in Example 1.

[0125] The valence comparisons are shown in Table 9:

[0126] Table 9

[0127]

[0128] Its storage and agglomeration effect is, for example, Figure 3 As shown.

[0129] From Table 9 and Figure 3 It is known that the amount of taurine added has no significant effect on the background, but without taurine, particulate aggregation is easily formed. Considering the small molecular weight of taurine, only a small amount is needed, and excessive amounts would be wasteful. Therefore, the recommended dosage is 5–10 mg / mL.

[0130] 2. Preparation of novel blocking solution: Prepare 1L of 0.05mol / L CB buffer; weigh 10g of methoxy polyethylene glycol amine (molecular weight 5000); the amount of taurine substitute is shown in Table 10. Add them to the CB buffer in sequence and mix until completely dissolved.

[0131] Table 10

[0132] Sealing liquid 1 Sealing liquid 2 Sealing liquid 3 Glycine - 5 mg / mL Glutamic acid - 5 mg / mL Aspartic acid - 5 mg / mL

[0133] The preparation process and performance testing scheme of magnetic microparticles are shown in Example 1.

[0134] Its storage and agglomeration effect is, for example, Figure 4 As shown.

[0135] From Table 10 and Figure 4 It is known that glycine also helps with aggregation, but not as much as taurine, while glutamic acid and aspartic acid do not help with aggregation. Therefore, taurine is the better choice.

[0136] Example 4

[0137] Taking CA153 coated magnetic microparticles as an example, this article details the application scope of CB buffer:

[0138] Prepare the following different buffer solutions (as shown in Table 11): 1L; 10g of methoxy polyethylene glycolamine (molecular weight 5000); and 5g of taurine. Add them to the buffer solutions in sequence and mix until completely dissolved.

[0139] Table 11

[0140] Sealing liquid 1 Sealing liquid 2 Sealing liquid 3 CB buffer 0.05 mol / L Purified water PBS buffer 0.05 mol / L

[0141] The preparation process and performance testing scheme of magnetic microparticles are shown in Example 1.

[0142] Table 12

[0143]

[0144] As shown in Table 12, alkaline CB buffer is a more important component of this blocking solution, while weakly acidic purified water and neutral PBS buffer are less effective.

[0145] As can be seen from all the above embodiments, the present invention provides a dual-function cleaning and blocking treatment solution and its application method for reducing non-specific adsorption and magnetic particle agglomeration. The solution includes the following steps: dissolving a certain amount of methoxy polyethylene glycol amine and taurine in CB buffer to prepare this novel carboxyl magnetic particle blocking solution. Using this blocking solution to block and clean magnetic particles coated with active materials for a certain period of time can reduce the background luminescence value of the coated magnetic particles to a certain extent, increase the luminescence gradient, and make the treated magnetic particles more resistant to long-term storage, reducing the agglomeration of particles generated during storage. This blocking solution is simple to use, significantly improves the performance of magnetic particles, and has high application value.

[0146] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A treatment solution for magnetic microparticles, characterized in that, Includes the composition, and acceptable excipients or additives; The composition comprises an alkaline buffer solution, a PEG-NH2 compound, and taurine; The alkaline buffer solution includes CB buffer solution; The PEG-NH2 compounds include one or more of methoxy polyethylene glycolamine, polyethylene glycolamine, or Y-type polyethylene glycolamine; The treatment fluid includes a sealing fluid, a cleaning fluid, or a dual-function cleaning and sealing treatment fluid.

2. The treatment solution as described in claim 1, characterized in that, include: The concentration of the alkaline buffer solution is 0.05 mol / L; and / or The concentration of the PEG-NH2 compound is 10~50 mg / mL.

3. The treatment solution as described in claim 1, characterized in that, include: The CB buffer comprises 1.59 g / L sodium carbonate and 2.93 g / L sodium bicarbonate; and / or The concentration of the methoxy polyethylene glycolamine is 10-50 mg / mL; and / or The concentration of taurine is 5~10 mg / mL.

4. The treatment solution as described in claim 1, characterized in that, include: The molecular weight of the methoxy polyethylene glycol amine is 2000~20000; and / or The polyethylene glycolamine has a molecular weight of 5000; and / or The molecular weight of the Y-type polyethylene glycolamine is 15,000.

5. The use of the treatment solution according to any one of claims 1 to 4 in any of the following: (I) Reduce the hydrophobic effect on the surface of magnetic particles; and / or (II) Reduce the non-specific adsorption of magnetic particles; and / or (III) Reduce the aggregation of magnetic particles during storage; and / or (IV) Enclosure of magnetic particles; and / or (V) Cleaning of magnetic particles; and / or (VI) Preparation of magnetic microparticle sealing solution; and / or (VII) Preparation of magnetic particle cleaning solution; and / or (VIII) Preparation of a dual-function treatment solution for cleaning and sealing magnetic microparticles.

6. The method for preparing the treatment liquid according to any one of claims 1 to 4, characterized in that, Includes the following steps: Step (1): Mix 1.59 g sodium carbonate, 2.93 g sodium bicarbonate and 1000 mL water to obtain CB buffer; Step (2): Take the CB buffer solution from step (1) and mix it evenly with 10 g of methoxy polyethylene glycolamine and 5 g of taurine to obtain the treatment solution.

7. A method for sealing magnetic microparticles, characterized in that, include: Mix the blocking reagent with the magnetic microparticles and react for 30-120 minutes to end the blocking process. The sealing reagent includes the treatment solution as described in any one of claims 1 to 4 or the treatment solution prepared by the preparation method as described in claim 6.

8. A method for cleaning magnetic particles, characterized in that, include: Mix the cleaning reagent with the magnetic microparticles, react for 10 min, remove the supernatant, repeat 3 times, and then end the cleaning process. The cleaning reagent includes the treatment solution as described in any one of claims 1 to 4 or the treatment solution prepared by the preparation method as described in claim 6.

9. A method for preparing magnetic microparticles of coupled active material, characterized in that, Includes the following steps: Step (a): Wash the magnetic particles with PBS buffer; Step (b): Mix the magnetic microparticles obtained in step (a) with EDC / NHS coupling agent to obtain activated magnetic microparticles; Step (c): Take MES buffer to wash the activated magnetic microparticles described in step (b), mix the activated magnetic microparticles with the activated material, remove the supernatant, and obtain unblocked magnetic microparticles; Step (d): Mix the blocking reagent with the unblocked magnetic microparticles described in step (c), react for 5-10 min, remove the supernatant, repeat 3 times to obtain the magnetic microparticles of the coupling active material; The sealing reagent includes the treatment solution as described in any one of claims 1 to 4 or the treatment solution prepared by the preparation method as described in claim 6; The living material includes mouse monoclonal antibodies.

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