一种高产L-高丝氨酸的无质粒重组大肠杆菌及其应用
By modifying Escherichia coli using metabolic engineering and gene editing technologies, a plasmid-free recombinant Escherichia coli with high L-homoserine production was constructed. This solved the problems of long reaction time, low yield, and difficult purification in chemical synthesis, achieving efficient production and simplified purification, making it suitable for industrial applications.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHEJIANG UNIV OF TECH
- Filing Date
- 2023-03-07
- Publication Date
- 2026-07-17
AI Technical Summary
Existing chemical methods for synthesizing L-homoserine suffer from problems such as long reaction time, low yield, and difficulty in purification. Furthermore, alkaline conditions during chemical synthesis can lead to changes in the product configuration, reducing the yield of the target product and increasing the difficulty of separation and purification.
By modifying Escherichia coli using metabolic engineering and gene editing technologies, a plasmid-free recombinant E. coli with high L-homoserine production was constructed. Key genes were replaced using CRISPR-Cas9 gene editing technology to enhance the synthesis capacity of L-homoserine, including replacing the fliK, ompT, yeeJ, yjiP, pntA-pntB, ltaE-poxB, yjiV, yeeL, and nuoG genes, and optimizing the metabolic pathway to increase L-homoserine production.
It has achieved high-yield production of L-homoserine, increasing fermentation yield to 75 g/L, reducing fermentation costs, simplifying product purification process, and making it suitable for industrial applications.
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