Multicolor high-speed fluorescence excitation light spectral microscopy system without reference spectrum
By using a broadband light source and an excitation wavelength selection device with an acousto-optic effect, combined with a mutual information minimization algorithm, multi-color high-speed fluorescence excitation spectral microscopy imaging without the need for a reference spectrum is achieved, solving the problems of slow imaging speed and large image decomposition errors in existing technologies, and achieving efficient sample decomposition and accurate image reconstruction.
CN116399843BActive Publication Date: 2025-10-17UNIV OF ELECTRONICS SCI & TECH OF CHINA
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Patent Information
- Application Number
- CN202310421506.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-04-19
- Publication Date
- 2025-10-17
- Estimated Expiration
- 2043-04-19
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Figure CN116399843B_ABST
Abstract
The application discloses a multi-color high-speed fluorescence excitation spectrum microscopic imaging system without reference spectrum, which comprises a broadband light source, an excitation wavelength selector, an excitation light expansion unit, a filter unit, a signal control and synchronization unit, a microscopic excitation and fluorescence detection system, and an image acquisition storage and image decomposition part. The application greatly simplifies the fluorescence excitation spectrum microscopic imaging system on the basis of a single light source, realizes the synchronization of the excitation wavelength and the camera and the rapid scanning, can perform high-speed wide-field imaging on a fluorescence sample, overcomes the bottleneck problem that the reference spectrum needs to be detected first in the traditional spectrum imaging, and significantly improves the image acquisition speed and the multi-color image decomposition precision.
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