An apparatus for adherent cell digestion in a sheet-shaped carrier cell culture bag

Abstract

The application discloses a device for adherent cell digestion in a sheet carrier cell culture bag, which comprises a device rack, an electric control bin, a waste liquid bin and a beating bin are arranged in the device rack, an industrial computer is arranged in the electric control bin, a waste liquid drawer is arranged in the waste liquid bin, a beating assembly is arranged in the beating bin, a bag clamping assembly is arranged on the upper part of the front side of the beating bin outside the device rack, a baffle assembly is arranged on the lower part of the front side of the beating bin outside the device rack, a bed is arranged on the top of the electric control bin outside the device rack, a temperature control box and a man-machine interaction panel are arranged on the top of the beating bin outside the device rack. The device has the advantages of simple operation, easy to use, which can greatly improve the digestion efficiency, ensure the stability of the digestion process, save labor, reduce pollution, and has strong universality and can be applied to the digestion of adherent cells in sheet carrier cell culture bags of various specifications.

Description

Technical Field

[0001] This invention relates to the field of cell digestion technology, specifically to a device for digesting adherent cells in sheet-like carrier cell culture bags. Background Technology

[0002] Currently, the digestion process of adherent cells in sheet-like cell culture bags on the market is all done manually. This involves manually adding various trypsin enzymes and serum-free culture medium to the sheet-like cell culture bag, then gently or manually tapping the surface of the culture bag to agitate the sheet-like carriers and promote the digestion of the adherent cells, thus causing them to detach from the carriers. However, this method generally has the following problems: 1. It is very inconvenient to operate, and it is time-consuming and labor-intensive; 2. It is greatly affected by human factors, has poor process stability, and does not comply with GMP (Good Manufacturing Practice) standards.

[0003] 3. It is easily affected by individual work experience, and the digestion time may be long or short, and the tapping force may be light or heavy, resulting in different actual digestion effects for the same batch of cells, which is not conducive to subsequent production or experiments.

[0004] 4. Since the required cell yield varies for each batch, manually tapping the cell culture bags of different sizes results in different digestion effects and significant inconsistencies. Summary of the Invention

[0005] The purpose of this invention is to provide a device for digesting adherent cells in a sheet-like carrier cell culture bag, thereby solving the problems existing in the manual digestion process of adherent cells in the prior art.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows: An apparatus for digesting adherent cells in a sheet-like cell culture bag includes an apparatus frame. Inside the apparatus frame are an electrical control chamber, a waste liquid chamber, and a tapping chamber. An industrial control computer is located inside the electrical control chamber. A waste liquid drawer is located inside the waste liquid chamber. A tapping assembly is located inside the tapping chamber. A bag clamping assembly is located outside the apparatus frame and at the upper front of the tapping chamber. A baffle assembly is located outside the apparatus frame and at the lower front of the tapping chamber. A swing bed is located outside the apparatus frame and at the top of the electrical control chamber. A temperature control box and a human-machine interface panel are located outside the apparatus frame and at the top of the tapping chamber. The human-machine interface panel is electrically connected to the industrial control computer. The industrial control computer is electrically connected to the tapping assembly, the bag clamping assembly, the swing bed, and the temperature control box. The waste liquid drawer is used to store disposable waste liquid bags; The tapping component is used to tap the disposable sheet-like carrier cell culture bag; The bag clamping assembly is used to hold disposable sheet-like carrier cell culture bags; The baffle assembly is used to hold the disposable sheet-like carrier cell culture bag in front of the tapping assembly; Among them, the stacking bed is used to place disposable cell product bags; The temperature control box is used to hold disposable culture medium bags and control their temperature.

[0007] Furthermore, the striking assembly includes a stepper motor, a lead screw, a lead screw connecting seat, a lead screw connecting block, a fixed base, a fixed connecting seat, a linear guide rail, a linear guide rail slider, a fixed top plate, a first servo motor, a second servo motor, a first motor connecting seat, a second motor connecting seat, a first swing arm, a second swing arm, a first connecting rod, a second connecting rod, a first beat, a second beat, a third beat, a fourth beat, a first beat guide shaft, a second beat guide shaft, a third beat guide shaft, a fourth beat guide shaft, a first beat counterweight, a second beat counterweight, a third beat counterweight, and a fourth beat counterweight. The stepper motor is fixed to the bottom wall of the striking chamber and connected to the industrial control computer; one end of the lead screw is connected to the stepper motor, and the other end is connected to the fixed base through the lead screw connecting seat; one end of the lead screw connecting block is threaded onto the lead screw, and the other end is fixed to the fixed connecting seat; there are two linear guides, which are respectively fixed to both ends of the upper part of the fixed base; there are two sets of linear guide sliders, which are respectively slidably mounted on the two linear guides; the bottom of the fixed connecting seat is fixed to the upper part of the two sets of linear guide sliders, and the top is fixed to the fixed top plate; the first servo motor is fixed to one end of the fixed top plate through the first motor connecting seat and connected to the industrial control computer; the second servo motor is fixed to the other end of the fixed top plate through the second motor connecting seat and connected to the industrial control computer; the first swing... One end of the arm is connected to the output end of the first servo motor, and the other end is connected to the first connecting rod. The other end of the first connecting rod is connected to the first beat and the third beat, respectively. One end of the second swing arm is connected to the output end of the second servo motor, and the other end is connected to the second connecting rod. The other end of the second connecting rod is connected to the second beat and the fourth beat, respectively. The first beat guide shaft, the second beat guide shaft, the third beat guide shaft, and the fourth beat guide shaft are all slidably supported on the fixed top plate by a sliding bushing. One end of the first beat guide shaft, the second beat guide shaft, the third beat guide shaft, and the fourth beat guide shaft are connected to the first beat, the second beat, the third beat, and the fourth beat, respectively, and the other end is connected to the first beat counterweight, the second beat counterweight, the third beat counterweight, and the fourth beat counterweight, respectively.

[0008] Furthermore, the bag clamping assembly includes a first bag clamping component, a second bag clamping component, a quick-release handle, a feeding handle, a clamping shaft, a hook, a weighing connection plate, and a weighing sensor; The weighing sensor is located in the middle of the rear side of the weighing connecting plate and passes through the inside of the tapping chamber, and is electrically connected to the industrial control computer; there are two hooks and they are respectively fixed at both ends of the front side of the weighing connecting plate; there are two clamping shafts and they are respectively hooked onto the two hooks; there are two feeding handles and they are respectively fixed at the upper part of the front ends of the first bag clamping component; the second bag clamping component is located at the lower part of the front side of the first bag clamping component and is locked to the first bag clamping component through the quick-tightening handle; the first bag clamping component is located on the front side of the weighing connecting plate, and its two ends are respectively fixedly connected to the two clamping shafts.

[0009] Furthermore, the baffle assembly includes a first baffle, a second baffle, a locking handle, a locking ring, an upper limit plate, a lower limit plate, a first rotating shaft, and a fixing rod; One end of the first baffle is sleeved on the first rotating shaft, and the other end is locked to the fixed rod by a locking handle; the second baffle is fixed to the rear side of the first baffle; there are two locking rings, which are respectively located on the upper and lower sides of the first baffle sleeved on one end of the first rotating shaft, and are both locked on the first rotating shaft; there are several upper limit plates, all of which can be slidably inserted through the upper part between the first baffle and the second baffle; there are several lower limit plates, all of which can be slidably inserted through the lower part between the first baffle and the second baffle; both ends of the first rotating shaft are respectively fixedly supported on one side of the equipment frame in front of the beater chamber by a first support rod; both ends of the fixed rod are respectively fixedly supported on the other side of the equipment frame in front of the beater chamber by a second support rod.

[0010] Furthermore, the swing bed includes a swing bed tray, and a manual clamping device is provided on the front and rear sides or left and right sides of the upper part of the swing bed tray. A swing bed top plate is provided at the bottom of the swing bed tray, and a swing bed cover is provided below the swing bed top plate. The swing bed cover is movably supported on the equipment frame at the top of the electrical control compartment. Two swing bed support seats are provided inside the swing bed cover, and the upper parts of the two swing bed support seats penetrate through the swing bed cover and are respectively hinged to the bottom of the swing bed top plate through a second rotating shaft. Inside the outer casing of the swing bed, there is a swing bed motor, a swing arm, a swing bed base plate, and four weighing modules. The swing bed motor is supported on the inner side of one of the swing bed support seats and is connected to the industrial control computer. The swing arm is located on the outer side of the swing bed support seat supporting the swing bed motor, with one end connected to the output end of the swing bed motor and the other end connected to the top plate of the swing bed. The swing bed base plate is fixed to the bottom of the two swing bed support seats. The four weighing modules are located at the four corners of the bottom of the swing bed base plate and are all connected to the industrial control computer.

[0011] Furthermore, the temperature control box includes a temperature control box body, a box cover is provided on the top of the temperature control box body, a tray is provided inside the temperature control box body, three nylon plates are provided on the upper part of the tray, a heating plate is provided on the upper part of each nylon plate, a temperature sensor is embedded in the middle of each heating plate, and a placement area for placing disposable culture medium bags is provided on the upper part of each heating plate. The heating plates and temperature sensors are electrically connected to the industrial control computer.

[0012] Furthermore, a first clamp valve, a second clamp valve, and a third clamp valve are sequentially arranged from left to right on the front side of the temperature control chamber. A first hose is provided on the first clamp valve, a second hose is provided on the second clamp valve, and a third hose is provided on the third clamp valve. One end of the first hose, the second hose, and the third hose are respectively connected to three disposable culture medium bags placed on the three heating plate placement areas, and the other end of each hose is connected to an inlet hose. The inlet hose is supported on a first peristaltic pump and is used to connect to the inlet of a disposable sheet-like carrier cell culture bag located on the front side of the tapping chamber. The first peristaltic pump is located on the front side of the equipment frame and is connected to the industrial control computer.

[0013] Furthermore, a fifth clamp valve, a fourth clamp valve, and a second peristaltic pump are sequentially arranged from left to right on the front side of the swing bed cover. A fifth hose is provided on the fifth clamp valve, a fourth hose is provided on the fourth clamp valve, and a liquid outlet hose is provided on the second peristaltic pump. One end of the liquid outlet hose is used to connect to the liquid outlet of a disposable sheet-like carrier cell culture bag located on the front side of the tapping chamber, and the other end is connected to the fourth hose and the fifth hose respectively. The other end of the fourth hose is used to connect to a disposable cell product bag placed on the swing bed, and the other end of the fifth hose is used to connect to a disposable waste bag placed in the waste liquid drawer. The second peristaltic pump is connected to the industrial control computer.

[0014] Furthermore, the three disposable culture medium bags placed on the three heating plate placement areas are, from left to right, a disposable culture medium bag containing phosphate buffer solution, a disposable culture medium bag containing trypsin, and a disposable culture medium bag containing serum-containing cell growth medium.

[0015] Furthermore, a caster wheel is provided at each of the four corners of the bottom of the equipment frame.

[0016] Compared with the prior art, the advantages of the present invention are: (1) The equipment is simple to operate and easy to learn. It can completely replace manual operation and greatly reduce the workload of operators. (2) The entire digestion process is automatically controlled by the equipment, which can not only greatly improve digestion efficiency, but also ensure the consistency of the digestion process. (3) It can be used for digestion of various disposable sheet-like carrier cell culture bags of 20L and below (including 20L), and can avoid the tubing on digestion bags of various sizes, greatly enhancing its versatility; (4) The frequency, rhythm and mode of tapping can be customized to deal with the digestion of different cells, which can make cell digestion more thorough and greatly improve the quality and efficiency of cell digestion.

[0017] (5) The entire digestion process is carried out in a closed environment, which reduces pollution.

[0018] (6) The equipment process parameters are stable, ensuring the stability of the entire cell digestion process; (7) It can be applied to the passage and expansion of adherent cells and the harvesting and recovery of some medicinal adherent cells. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in this embodiment, the accompanying drawings used in the description of the embodiment will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is an embodiment of the device of the present invention for digesting adherent cells in a sheet-like carrier cell culture bag; Figure 2 yes Figure 1 Top view of the Chinese embodiment; Figure 3 yes Figure 1 Explosion diagram of the Chinese embodiment; Figure 4 yes Figure 2 Sectional view of AA; Figure 5 yes Figure 2 BB section view; Figure 6 This is a structural diagram of the tapping component; Figure 7 This is a top view of the tapping component; Figure 8 yes Figure 7 CC section view; Figure 9 yes Figure 7 DD section view; Figure 10 yes Figure 7 EE section view; Figure 11 yes Figure 7 FF section view; Figure 12 This is a schematic diagram of the bag clamping assembly; Figure 13 This is an exploded view of the bag clamping assembly; Figure 14 This is a schematic diagram of the assembly of the bag clamping assembly and the disposable sheet-like carrier cell culture bag; Figure 15 This is a structural schematic diagram of the baffle assembly; Figure 16 This is an assembly diagram of the baffle assembly and the equipment frame; Figure 17 yes Figure 16 A partial schematic diagram; Figure 18 This is a structural diagram of a bed; Figure 19 This is a top view of the bed arrangement; Figure 20 yes Figure 19 Middle GG sectional view; Figure 21 yes Figure 19 Middle HH sectional view; Figure 22 This is a structural diagram of the temperature control box; Figure 23 This is a partial structural diagram of the temperature control box; Figure 24 This is a top view of the temperature control box; Figure 25 yes Figure 24 Sectional view II; Figure 26 yes Figure 24 Sectional view of JJ; Figure 27 This is a flowchart of the cell digestion process; Explanation of reference numerals in the attached drawings: 1. Equipment frame; 2. Electrical control compartment; 3. Waste liquid compartment; 4. Beating compartment; 5. Waste liquid drawer; 6. Beating assembly; 601. Stepper motor; 602. Lead screw; 603. Lead screw connecting seat; 604. Lead screw connecting block; 605. Fixed base; 606. Fixed connecting seat; 607. Linear guide rail; 608. Linear guide rail slider; 609. Fixed top plate; 610. First servo motor; 611. Second servo motor; 612. First motor connecting seat; 613. Second motor connecting seat; 614. First swing arm; 615. Second swing arm; 616. First connecting rod; 617. Second... Connecting rod; 618, First beat; 619, Second beat; 620, Third beat; 621, Fourth beat; 622, First beat guide shaft; 623, Second beat guide shaft; 624, Third beat guide shaft; 625, Fourth beat guide shaft; 626, First beat counterweight; 627, Second beat counterweight; 628, Third beat counterweight; 629, Fourth beat counterweight; 630, Sliding bushing; 7, Bag clamping assembly; 701, First bag clamping component; 702, Second bag clamping component; 703, Quick-tension handle; 704, Feeding handle; 705, Clamping shaft; 706, Hook; 707 708. Weighing connection plate; 809. Weighing sensor; 8000. Baffle assembly; 801. First baffle; 802. Second baffle; 803. Locking handle; 804. Locking ring; 805. Upper limit plate; 806. Lower limit plate; 807. First rotating shaft; 808. Fixing rod; 809. First support rod; 810. Second support rod; 901. Swing bed; 902. Swing bed tray; 903. Manual clamping component; 904. Swing bed top plate; 905. Swing bed outer cover; 906. Swing bed support seat; 907. Second rotating shaft; 908. Swing bed motor; 909. Swing bed base plate; 910. Weighing module 10. Temperature control box; 1001 Temperature control box body; 1002 Box lid; 1003 Tray; 1004 Nylon sheet; 1005 Heating plate; 1006 Temperature sensor; 11 Human-machine interface panel; 12 First clamp valve; 13 Second clamp valve; 14 Third clamp valve; 15 First peristaltic pump; 16 Second peristaltic pump; 17 Fourth clamp valve; 18 Fifth clamp valve; 19 First hose; 20 Second hose; 21 Third hose; 22 Inlet hose; 23 Outlet hose; 24 Fourth hose; 25 Fifth hose; 26 Casters; 27 Hose bracket; 100. Disposable culture medium bag; 200. Disposable sheet-like carrier cell culture bag; 300. Disposable finished cell bag; 400. Disposable waste liquid bag. Detailed Implementation

[0021] To make the technical means, creative features, objectives and effects of this invention easier to understand, the following description, in conjunction with the accompanying drawings and specific embodiments, further explains how this invention is implemented.

[0022] See Figures 1 to 5 The present invention relates to an apparatus for digesting adherent cells in a sheet-like carrier cell culture bag, comprising an apparatus frame 1, an electrical control chamber 2, a waste liquid chamber 3, and a tapping chamber 4 disposed inside the apparatus frame 1, an industrial control computer (not shown in the figure) disposed inside the electrical control chamber 2, a waste liquid drawer 5 disposed inside the waste liquid chamber 3, a tapping component 6 disposed inside the tapping chamber 4, a bag clamping component 7 disposed outside the apparatus frame 1 and located at the upper front side of the tapping chamber 4, a baffle component 8 disposed outside the apparatus frame 1 and located at the lower front side of the tapping chamber 4, a swing bed 9 disposed outside the apparatus frame 1 and located at the top of the electrical control chamber 2, a temperature control box 10 and a human-machine interface panel 11 disposed outside the apparatus frame 1 and located at the top of the tapping chamber 4, wherein the human-machine interface panel 11 is electrically connected to the industrial control computer, and the industrial control computer is electrically connected to the tapping component 6, the bag clamping component 7, the swing bed 9 and the temperature control box 10 respectively. Among them, waste liquid drawer 5 is used to hold disposable waste liquid bags 400; Among them, the tapping component 6 is used to tap the disposable sheet-like carrier cell culture bag 200, so that the adherent cells inside the disposable sheet-like carrier cell culture bag 200 detach from the sheet-like carrier; The bag clamping assembly 7 is used to clamp the disposable sheet-like carrier cell culture bag 200, so that the disposable sheet-like carrier cell culture bag 200 is suspended to the front of the tapping assembly 6; Among them, the baffle assembly 8 is used to block the disposable sheet-like carrier cell culture bag 200 in front of the tapping assembly 6 to prevent the disposable sheet-like carrier cell culture bag 200 from swinging forward when the tapping assembly 6 taps the disposable sheet-like carrier cell culture bag 200 forward. The 9-bed is used to hold the disposable cell product bag 300 to prevent the finished cells flowing into the disposable cell product bag 300 from settling and clumping. The temperature control box 10 is used to hold the disposable culture medium bag 100 and control the temperature of the disposable culture medium bag 100. The human-computer interaction panel 11 is used for human-computer interaction to control the operation of the entire digestion equipment.

[0023] Specifically, as an embodiment of the present invention: the waste liquid tank 3 is located on one side of the top of the electrical control tank 2, the swing bed 9 is located on the other side of the top of the electrical control tank 2 and is arranged side by side with the waste liquid tank 3, the tapping tank 4 is located on top of the waste liquid tank 3, one end of the temperature control box 10 is supported on the equipment frame 1 on the top of the tapping tank 4, and the other end is suspended on the equipment frame 1 above the swing bed 9.

[0024] Specifically, as one embodiment of the present invention: see Figures 6 to 11 The striking assembly 6 includes a stepper motor 601, a lead screw 602, a lead screw connecting seat 603, a lead screw connecting block 604, a fixed base 605, a fixed connecting seat 606, a linear guide rail 607, a linear guide rail slider 608, a fixed top plate 609, a first servo motor 610, a second servo motor 611, a first motor connecting seat 612, a second motor connecting seat 613, a first swing arm 614, a second swing arm 615, a first connecting rod 616, a second connecting rod 617, a first beat 618, a second beat 619, a third beat 620, a fourth beat 621, a first beat guide shaft 622, a second beat guide shaft 623, a third beat guide shaft 624, a fourth beat guide shaft 625, a first beat counterweight 626, a second beat counterweight 627, a third beat counterweight 628, and a fourth beat counterweight 629. Among them, the stepper motor 601 is fixed on the bottom wall of the tapping chamber 4 and connected to the industrial control computer; one end of the lead screw 602 is connected to the output end of the stepper motor 601, and the other end is connected to the fixed base 605 through the lead screw connecting seat 603; one end of the lead screw connecting block 604 is threaded on the lead screw 602, and the other end is fixed on the fixed connecting seat 606. There are two linear guide rails 607, which are respectively fixed at both ends of the upper part of the fixed base 605; there are two sets of linear guide rail sliders 608, which are respectively slidably mounted on the two linear guide rails 607. The bottom of the fixed connecting seat 606 is fixed to the upper part of the two sets of linear guide sliders 608, and the top is fixed to the fixed top plate 609. A first servo motor 610 is set at one end of the fixed top plate 609, and a second servo motor 611 is set at the other end. The first servo motor 610 is fixedly connected to the upper part of one end of the fixed top plate 609 through the first motor connecting seat 612 and is connected to the industrial control computer. The second servo motor 611 is fixedly connected to the lower part of the other end of the fixed top plate 609 through the second motor connecting seat 613 and is connected to the industrial control computer. The first swing arm 614 is located on one side of the first servo motor 610, with one end connected to the output end of the first servo motor 610 and the other end connected to the first connecting rod 616. The other end of the first connecting rod 616 is connected to the first beat 618 and the third beat 620 respectively. The second swing arm 615 is located on one side of the second servo motor 611, with one end connected to the output end of the second servo motor 611 and the other end connected to the second connecting rod 617. The other end of the second connecting rod 617 is connected to the second beat 619 and the fourth beat 621 respectively. The first beat guide shaft 622, the second beat guide shaft 623, the third beat guide shaft 624 and the fourth beat guide shaft 625 are all slidably supported on the fixed top plate 609 by a sliding bushing 630. The front ends of the first beat guide shaft 622, the second beat guide shaft 623, the third beat guide shaft 624 and the fourth beat guide shaft 625 are respectively connected to the first beat 618, the second beat 619, the third beat 620 and the fourth beat 621, and the rear ends are respectively connected to the first beat counterweight 626, the second beat counterweight 627, the third beat counterweight 628 and the fourth beat counterweight 629. Among them, the first counterweight 626, the second counterweight 627, the third counterweight 628 and the fourth counterweight 629 have the same weight as the corresponding front-end counterweight, which is used to ensure the stability of the equipment when the counterweights are staggered.

[0025] The stepper motor 601 can control the relative distance between the four beats and the disposable sheet-like carrier cell culture bag 200 through the connected lead screw assembly and linear guide assembly, so as to adjust the impact force of the four beats when they come into contact with the disposable sheet-like carrier cell culture bag 200, thereby meeting different process requirements; the first servo motor 610 can control the first beat 618 and the third beat 620 to perform the beating action through the first swing arm 614 and the first connecting rod 616; the second servo motor 611 can control the second beat 619 and the fourth beat 621 to perform the beating action through the second swing arm 615 and the second connecting rod 617.

[0026] During operation, the first servo motor 610 and the second servo motor 611 can be set to start synchronously to make the four beats run simultaneously (i.e., synchronized beats), and the first servo motor 610 and the second servo motor 611 can be started at intervals to make the four beats move alternately in pairs (i.e., staggered beats).

[0027] Specifically, as one embodiment of the present invention: see Figures 12 to 13 The bag clamping assembly 7 includes a first bag clamping component 701, a second bag clamping component 702, a quick-release handle 703, a feeding handle 704, a clamping shaft 705, a hook 706, a weighing connection plate 707, and a weighing sensor 708. The weighing sensor 708 is located in the middle of the rear side of the weighing connection plate 707 and passes through the inside of the tapping chamber 4. It is connected to the industrial control computer and is used to detect the volume of liquid entering and exiting the disposable sheet-like carrier cell culture bag 200, so as to facilitate system control. Among them, there are two hooks 706, which are fixed on the left and right ends of the front side of the weighing connecting plate 707 respectively, and there are two clamping shafts 705, which are hooked on the two hooks 706 respectively. Among them, there are two feeding handles 704, which are respectively fixed on the upper part of the left and right ends of the front side of the first bag clamping component 701, so as to facilitate the use of both hands to grasp the feeding handles 704 and hook the disposable sheet-shaped carrier cell culture bag 200 onto the hook 706 through the clamping shaft 705. The second clamping bag 702 is located at the lower front side of the first clamping bag 701 and is locked to the first clamping bag 701 via a quick-tightening handle 703. The first clamping bag 701 is located at the front side of the weighing connecting plate 707, and its two ends are fixedly connected to two clamping shafts 705 respectively. The second clamping bag 702 is used to loosen or tighten the first clamping bag 701 via the quick-tightening handle 703, thereby realizing the loosening or tightening operation of the disposable sheet-like carrier cell culture bag 200.

[0028] Specifically, as one embodiment of the present invention: see Figures 14 to 17 The baffle assembly 8 includes a first baffle 801, a second baffle 802, a locking handle 803, a locking ring 804, an upper limit plate 805, a lower limit plate 806, a first rotating shaft 807, and a fixing rod 808. The first baffle 801 is fitted onto the first rotating shaft 807 at one end, and locked onto the fixed rod 808 at the other end by a locking handle 803; the second baffle 80 is fixed to the rear side of the first baffle 801; there are two locking rings 804, which are respectively located on the upper and lower sides of the first baffle 80 fitted onto the first rotating shaft 807 at one end, and are both locked onto the first rotating shaft 807; there are several upper limit plates 805, which can all slide through the upper part between the first baffle 801 and the second baffle 802; there are several lower limit plates 806, which can all slide through the lower part between the first baffle 801 and the second baffle 802; the two ends of the first rotating shaft 807 are respectively fixedly supported on one side of the equipment frame 1 in front of the beater chamber 4 by a first support rod 809; the two ends of the fixed rod 808 are respectively fixedly supported on the other side of the equipment frame 1 in front of the beater chamber 4 by a second support rod 810.

[0029] The first baffle 801 is used to hold the disposable sheet-like carrier cell culture bag 200 in front of the tapping component 6. The second baffle 802 cooperates with the first baffle 801 to clamp the upper limit plate 805 and the lower limit plate 806. The upper limit plate 805 and the lower limit plate 806 further hold the disposable sheet-like carrier cell culture bag 200. In addition, the upper limit plate 805 and the lower limit plate 806 are slidably inserted between the first baffle 801 and the second baffle 802 to facilitate adjusting their position to avoid obstructing the disposable sheet-like carrier cell culture bag 200. The digestive bag 200 has pre-welded pipes or connectors; the first rotating shaft 807 is for convenient outward or inward rotation of the first baffle 801; the locking handle 803 is for convenient loosening or locking of the first baffle 801; the locking ring 804 can be used to adjust the up and down position of the entire baffle assembly 8. When the locking ring 804 is loosened, the first baffle 801 can move up and down along the first rotating shaft 807. When the locking ring 804 is locked, the first baffle 801 is blocked by the upper and lower locking rings 804 and can no longer move up and down on the first rotating shaft 807.

[0030] Specifically, as one embodiment of the present invention: see Figures 18 to 21 The swing bed 9 includes a swing bed tray 901. A manual clamping element 902 is provided on each of the front and rear (or left and right) sides of the upper part of the swing bed tray 901. A swing bed top plate 903 is provided at the bottom of the swing bed tray 901. A swing bed cover 904 is provided below the swing bed top plate 903, and the swing bed cover 904 is movably supported on the equipment frame 1 at the top of the electrical control compartment 2. Two swing bed support seats 905 are provided inside the swing bed cover 904, and the upper parts of the two swing bed support seats 905 penetrate through the swing bed cover 904 and are respectively hinged to the bottom of the swing bed top plate 903 via a second rotating shaft 906. The 04 also includes a swing bed motor 907, a swing arm 908, a swing bed base plate 909, and four weighing modules 910. The swing bed motor 907 is supported on the inner side of one of the swing bed support seats 905 and is connected to the industrial control computer. The swing arm 908 is located on the outer side of the swing bed support seat 905 that supports the swing bed motor 907, with one end connected to the output end of the swing bed motor 907 and the other end connected to the top plate 903 of the swing bed. The swing bed base plate 909 is fixed to the bottom of the two swing bed support seats 905. The four weighing modules 910 are located at the four corners of the bottom of the swing bed base plate 909 and are all connected to the industrial control computer.

[0031] More specifically, the manual clamping component 902 consists of a pressure plate 902a, a pressure handle 902b, and a screw 902c. The pressure plate 902a presses against the bed tray 901, the pressure plate 902b is hinged to the upper part of the screw 902c, and the lower part of the screw 902c passes through the pressure plate 902a and is threaded into the bed tray 901. The function of the manual clamping component 902 is to manually clamp the sequentially placed disposable cell product bags 300 on the bed tray 901.

[0032] Specifically, as one embodiment of the present invention: see Figures 22 to 26 The temperature control chamber 10 includes a temperature control chamber body 1001, a cover 1002 on the top of the temperature control chamber body 1001, a tray 1003 inside the temperature control chamber body 1001, three nylon plates 1004 on the upper part of the tray 1003, a heating plate 1005 on the upper part of each nylon plate 1004, a temperature sensor 1006 embedded in the middle of each heating plate 1005, and a placement area for placing disposable culture medium bags 100 on the upper part of each heating plate 1005. The heating plates 1005 and the temperature sensors 1006 are electrically connected to an industrial control computer. The heating plates 1005 are used to heat the temperature control chamber 10 and control the temperature inside the temperature control chamber 10; the temperature sensors 1006 are used to monitor the temperature inside the temperature control chamber 10; and the nylon plates 1004 are used for insulation to prevent heat dissipation inside the temperature control chamber 10.

[0033] Specifically, as one embodiment of the present invention: see Figure 22 and Figure 27 A first clamp valve 12, a second clamp valve 13, and a third clamp valve 14 are arranged sequentially from left to right on the front side of the temperature control chamber 1001. A first hose 19 is provided on the first clamp valve 12, a second hose 20 is provided on the second clamp valve 13, and a third hose 21 is provided on the third clamp valve 14. One end of the first hose 19, the second hose 20, and the third hose 21 are respectively connected to three disposable culture medium bags 100 placed on the three heating plates 1005, and the other end is connected to the liquid inlet hose 22. The liquid inlet hose 22 is supported on the first peristaltic pump 15 and is used to connect to the liquid inlet of the disposable sheet-like carrier cell culture bag 200 located on the front side of the tapping chamber 4. The first peristaltic pump 15 is located on the front side of the equipment frame 1 and is connected to the industrial control computer.

[0034] Specifically, as one embodiment of the present invention: see Figure 18 and Figure 27A fifth clamp valve 18, a fourth clamp valve 17 and a second peristaltic pump 16 are arranged sequentially from left to right on the front side of the outer cover 904 of the swing bed. A fifth hose 25 is arranged on the fifth clamp valve 18, a fourth hose 24 is arranged on the fourth clamp valve 17, and a liquid outlet hose 23 is arranged on the second peristaltic pump 16. One end of the outlet tube 23 is connected to the outlet of the disposable sheet-like carrier cell culture bag 200 located in front of the tapping chamber 4, and the other end is connected to the fourth tube 24 and the fifth tube 25 respectively. The other end of the fourth tube 24 is connected to the disposable cell product bag 300 placed on the swing bed 9, and the other end of the fifth tube 25 is connected to the disposable waste liquid bag 400 placed in the waste liquid drawer 5. The second peristaltic pump 16 is connected to the industrial control computer.

[0035] More specifically, in the above embodiments, see [reference] Figure 26 The three disposable culture medium bags 100 placed on the three heating plates 1005 are, from left to right, a disposable culture medium bag 100 containing culture medium, a disposable culture medium bag 100 containing trypsin, and a disposable culture medium bag 100 containing serum.

[0036] Specifically, as one embodiment of the present invention: see Figure 1 To facilitate moving the entire device, a caster 26 is installed at each of the four corners of the bottom of the device frame 1.

[0037] Specifically, as one embodiment of the present invention: see Figure 4 A corresponding hose bracket 27 is also provided on the front side of the equipment frame 1 to support the hose (e.g., the fifth hose 25) located on the front side of the equipment frame 1.

[0038] Specifically, in this invention, the waste liquid drawer 5 can hold a maximum of 20L disposable waste liquid bag 400; the bag clamping assembly 7 can be used to clamp disposable sheet-like carrier cell culture bags 200 of various specifications such as 2L, 5L, 10L, and 20L; the temperature control box 10 can hold a maximum of 3 20L disposable culture medium bags 100 at the same time; and the bed tray 901 can be selected from three specifications: 10L, 20L, and 50L.

[0039] Specifically, in this invention, the first tubing 19, the second tubing 20, the third tubing 21, the inlet tubing 22, the outlet tubing 23, the fourth tubing 24, and the fifth tubing 25 are all thermoplastic tubing.

[0040] The working process of the device for digesting adherent cells in a sheet-like carrier cell culture bag provided by the present invention is as follows: Step 1: Open the temperature control box 10, place three disposable culture medium bags 100 containing phosphate buffer solution, trypsin and serum-containing cell growth medium into the temperature control box 10, and start the heating function of the temperature control box 10 (i.e. start the heating plate 1005 to work) to set the temperature of the temperature control box 10 to 37℃ (i.e. use the temperature sensor 1006 to monitor the specific temperature value). The second step is to clamp the disposable sheet-like carrier cell culture bag 200 to be digested onto the bag clamping assembly 7, place the blank disposable cell finished product bag 300 onto the swing bed tray 901 of the swing bed 9, and put the blank disposable waste liquid bag 400 into the waste liquid drawer 7. Step 3: Using a professional tool (e.g., a pipe welding machine), connect the first tubing 19 to the disposable culture medium bag 100 containing phosphate buffer solution, connect the second tubing 20 to the disposable culture medium bag 100 containing trypsin, connect the third tubing 21 to the disposable culture medium bag 100 containing serum-containing cell growth medium, connect the inlet tubing 22 to the inlet of the disposable sheet-like carrier cell culture bag 200 to be digested, connect the outlet tubing 23 to the outlet of the disposable sheet-like carrier cell culture bag 200 to be digested, connect the fourth tubing 24 to the blank disposable finished cell bag 300, and connect the fifth tubing 25 to the blank disposable waste bag 400. Step 4: Select the appropriate digestion program on the human-computer interaction panel 11 and click the start button to begin the cell digestion process; Step 5: Open the first clamp valve 12 and start the first peristaltic pump 15 and weighing sensor 708 to allow the liquid in the disposable culture medium bag 100 containing phosphate buffered saline solution to flow through the first tubing 19 and the inlet tubing 22 into the disposable sheet-like carrier cell culture bag 200. Once the specified value (manually set) is reached, stop the first peristaltic pump 15 and weighing sensor 708, and close the first clamp valve 12. The "specified value" in this step specifically refers to the volume of phosphate buffered saline solution added to the disposable sheet-like carrier cell culture bag 200, which can be monitored by the weighing sensor 708. That is, the weighing sensor 708 can detect how much weight the disposable sheet-like carrier cell culture bag 200 has increased (i.e., how many grams of phosphate buffered saline solution have been added), and after proportional conversion, the volume of phosphate buffered saline solution added can be determined. Step 6: Activate the tapping component 6. The tapping component 6 taps the disposable sheet-like carrier cell culture bag 200 at a low frequency (i.e., all four taps simultaneously adhere to the surface of the bag) for a certain period (the tapping distance, frequency, and time are manually set) to achieve a cleaning effect. Then, turn off the tapping component 6. The purpose of this step is to clean the culture medium from the previous process from the sheet-like carrier inside the disposable sheet-like carrier cell culture bag 200. Specifically, this is done by adding a phosphate buffer solution to the disposable sheet-like carrier cell culture bag 200 to lower the pH concentration of the liquid inside the bag, thus achieving a cleaning effect on the sheet-like carrier. Step 7: Open the fifth clamp valve 18 and start the second peristaltic pump 16 and weighing sensor 708 to allow the liquid in the disposable sheet-like carrier cell culture bag 200 to flow through the outlet tube 23 and the fifth tube 25 into the blank disposable waste liquid bag 400. Once the specified value (manually set) is reached, stop the second peristaltic pump 16 and weighing sensor 708, and close the fifth clamp valve 18. The "specified value" in this step is specifically the volume of phosphate buffer solution that has decreased in the disposable sheet-like carrier cell culture bag 200, which can be monitored by the weighing sensor 708. That is, the weighing sensor 708 can detect how much weight the disposable sheet-like carrier cell culture bag 200 has decreased (i.e., how many grams of phosphate buffer solution have been reduced), and after proportional conversion, the volume of phosphate buffer solution that has decreased can be determined. Step 8: Open the second clamp valve 13 and start the first peristaltic pump 15 and weighing sensor 708 to allow the trypsin solution in the disposable culture medium bag 100 containing trypsin to flow through the second tubing 20 and the inlet tubing 22 into the disposable sheet-like carrier cell culture bag 200. Once the specified value (manually set) is reached, stop the first peristaltic pump 15 and weighing sensor 708, and close the second clamp valve 13. The "specified value" in this step specifically refers to how much trypsin solution has been added to the disposable sheet-like carrier cell culture bag 200, which can be monitored by the weighing sensor 708. That is, the weighing sensor 708 can detect how much weight (i.e. how many grams of trypsin solution) has been added to the disposable sheet-like carrier cell culture bag 200, and after proportional conversion, the volume of trypsin solution added can be determined. Step 9: Activate the tapping component 6. The tapping component 6 taps the disposable sheet-like carrier cell culture bag 200 at low frequency (1st and 3rd beats and 2nd and 4th beats alternately at low frequency) for a certain period of time (the tapping distance, frequency and time are manually set) to achieve the effect of mixing the trypsin with the sheet-like carrier inside the disposable sheet-like carrier cell culture bag 200. Then turn off the tapping component 6. Step 10: Open the third clamp valve 14 and start the first peristaltic pump 15 and weighing sensor 708. This allows the serum liquid in the disposable culture medium bag 100 containing serum-containing cell growth medium to flow through the third tubing 21 and the inlet tubing 22 into the disposable sheet-like carrier cell culture bag 200. Once the specified value (manually set) is reached, stop the first peristaltic pump 15 and weighing sensor 708, and close the third clamp valve 14. The "specified value" in this step specifically refers to the amount of serum-containing cell growth medium solution added to the disposable sheet-like carrier cell culture bag 200, which can be monitored by the weighing sensor 708. In other words, the weighing sensor 708 can detect how much weight (i.e., how many grams of serum-containing cell growth medium solution) has been added to the disposable sheet-like carrier cell culture bag 200. After proportional conversion, the volume of serum-containing cell growth medium solution added can be determined. Step 11: Start the tapping component 6. The tapping component 6 taps the disposable sheet-like carrier cell culture bag 200 at a high frequency (1st and 3rd taps and 2nd and 4th taps are alternately tapped at a high frequency to adhere to the surface of the disposable sheet-like carrier cell culture bag 200) for a certain period of time (the tapping distance, frequency and time are manually set) to achieve the effect of mixing the trypsin, serum-containing cell growth culture medium with the sheet-like carrier in the disposable sheet-like carrier cell culture bag 200. Then turn off the tapping component 6. Step 12: Open the fourth clamp valve 17 and start the second peristaltic pump 16 and weighing sensor 708 to allow the liquid in the disposable sheet-like carrier cell culture bag 200 to flow through the outlet tube 23 and the fourth tube 24 into the blank disposable cell product bag 300. After reaching the specified value (manually set), stop the second peristaltic pump 16 and weighing sensor 708, and close the fourth clamp valve 17. In this step, the "specified value" specifically refers to how much of the mixed solution of trypsin and serum-containing cell growth medium has been reduced in the disposable sheet-like carrier cell culture bag 200, which can be monitored by the weighing sensor 708. That is, the weighing sensor 708 can monitor how much weight the disposable sheet-like carrier cell culture bag 200 has lost (i.e., how many grams of the mixed solution of trypsin and serum-containing cell growth medium has been reduced). After proportional conversion, the volume of trypsin and serum-containing cell growth medium solution that has been reduced can be determined. Step 13: Start the swing bed 9 to begin operation; Step 14: Repeat steps 8 to 13 above until the specified number of times (manually set) is reached, and the digestion process of adherent cells in the disposable sheet-like carrier cell culture bag 200 will be completed. Step 15: Remove the disposable cell product bag 300 from the bed 9 to allow the product to enter the next process flow, such as cell stock solution dispensing.

[0041] Finally, it should be noted that the above description is only an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. An apparatus for digesting adherent cells in a sheet-like carrier cell culture bag, comprising an apparatus frame (1), characterized in that: An electrical control compartment (2), a waste liquid compartment (3), and a tapping compartment (4) are provided inside the equipment frame (1). An industrial control computer is provided inside the electrical control compartment (2). A waste liquid drawer (5) is provided inside the waste liquid compartment (3). A tapping assembly (6) is provided inside the tapping compartment (4). A bag clamping assembly (7) is provided outside the equipment frame (1) and on the upper front side of the tapping compartment (4). A baffle assembly (8) is provided on the lower side. A swing bed (9) is provided outside the equipment frame (1) and on top of the electrical control compartment (2). A temperature control box (10) and a human-machine interface panel (11) are provided outside the equipment frame (1) and on top of the tapping compartment (4). The human-machine interface panel (11) is electrically connected to the industrial computer. The industrial computer is electrically connected to the tapping assembly (6), the bag clamping assembly (7), the swing bed (9), and the temperature control box (10) respectively. Among them, the waste liquid drawer (5) is used to place disposable waste liquid bags (400); Among them, the tapping component (6) is used to tap the disposable sheet-like carrier cell culture bag (200). Among them, the bag clamping assembly (7) is used to clamp the disposable sheet-like carrier cell culture bag (200). Among them, the baffle assembly (8) is used to baffle the disposable sheet-like carrier cell culture bag (200) on the front side of the tapping assembly (6); Among them, the stacking bed (9) is used to place disposable cell product bags (300); The temperature control box (10) is used to place disposable culture medium bags (100) and control the temperature of the disposable culture medium bags (100); The bag clamping assembly (7) includes a first bag clamping component (701), a second bag clamping component (702), a quick-release handle (703), a feeding handle (704), a clamping shaft (705), a hook (706), a weighing connection plate (707), and a weighing sensor (708). The weighing sensor (708) is located in the middle of the rear side of the weighing connection plate (707), passes through the inside of the tapping chamber (4), and is connected to the industrial control computer. There are two hooks (706) and they are respectively fixed at both ends of the front side of the weighing connecting plate (707); There are two clamping shafts (705), which are respectively hooked onto the two hooks (706); There are two feeding handles (704) and they are respectively fixed on the upper part of the front two ends of the first bag clamp (701); The second clamping bag (702) is located at the lower front side of the first clamping bag (701) and is locked to the first clamping bag (701) by the quick-tight handle (703); The first clamping bag component (701) is located in front of the weighing connecting plate (707), and its two ends are fixedly connected to the two clamping shafts (705) respectively.

2. The apparatus for digesting adherent cells in a sheet-like carrier cell culture bag according to claim 1, characterized in that: The tapping assembly (6) includes a stepper motor (601), a lead screw (602), a lead screw connector (603), a lead screw connector (604), a fixed base (605), a fixed connector (606), a linear guide rail (607), a linear guide rail slider (608), a fixed top plate (609), a first servo motor (610), a second servo motor (611), a first motor connector (612), a second motor connector (613), a first swing arm (614), and a second swing arm (615). 615), first link (616), second link (617), first beat (618), second beat (619), third beat (620), fourth beat (621), first beat guide axis (622), second beat guide axis (623), third beat guide axis (624), fourth beat guide axis (625), first beat counterweight (626), second beat counterweight (627), third beat counterweight (628) and fourth beat counterweight (629); The stepper motor (601) is fixed on the bottom wall of the tapping chamber (4) and connected to the industrial control electromechanical system; One end of the lead screw (602) is connected to the stepper motor (601), and the other end is connected to the fixed base (605) through the lead screw connecting seat (603); One end of the lead screw connecting block (604) is threaded onto the lead screw (602), and the other end is fixed onto the fixed connecting seat (606); There are two linear guides (607), which are respectively fixed at both ends of the upper part of the fixed base (605); The linear guide slider (608) has two sets and is respectively slidably mounted on the two linear guides (607); The bottom of the fixed connecting seat (606) is fixed to the upper part of the two sets of linear guide sliders (608), and the top is fixed to the fixed top plate (609). The first servo motor (610) is fixed to one end of the fixed top plate (609) through the first motor connector (612) and is connected to the industrial control computer. The second servo motor (611) is fixed to the other end of the fixed top plate (609) via the second motor connector (613) and is electrically connected to the industrial control computer; One end of the first swing arm (614) is connected to the output end of the first servo motor (610), and the other end is connected to the first connecting rod (616). The other end of the first connecting rod (616) is connected to the first beat (618) and the third beat (620) respectively. One end of the second swing arm (615) is connected to the output end of the second servo motor (611), and the other end is connected to the second connecting rod (617). The other end of the second connecting rod (617) is connected to the second beat (619) and the fourth beat (621) respectively. The first beat guide shaft (622), the second beat guide shaft (623), the third beat guide shaft (624) and the fourth beat guide shaft (625) are all slidably supported on the fixed top plate (609) by a sliding bushing (630). One end of the first beat guide shaft (622), the second beat guide shaft (623), the third beat guide shaft (624) and the fourth beat guide shaft (625) are respectively connected to the first beat (618), the second beat (619), the third beat (620) and the fourth beat (621), and the other end is respectively connected to the first beat counterweight (626), the second beat counterweight (627), the third beat counterweight (628) and the fourth beat counterweight (629).

3. The apparatus for digesting adherent cells in a sheet-like carrier cell culture bag according to claim 1, characterized in that: The baffle assembly (8) includes a first baffle (801), a second baffle (802), a locking handle (803), a locking ring (804), an upper limit plate (805), a lower limit plate (806), a first rotating shaft (807), and a fixing rod (808). One end of the first baffle (801) is sleeved on the first rotating shaft (807), and the other end is locked to the fixed rod (808) by the locking handle (803); The second baffle (802) is fixed to the rear side of the first baffle (801); There are two locking rings (804), which are respectively disposed on the upper and lower sides of one end of the first baffle (801) and locked on the first rotating shaft (807); The upper limit plate (805) is multiple and can be slidably inserted in the upper part between the first baffle (801) and the second baffle (802); There are several lower limit plates (806), and each of them can be slidably inserted into the lower part between the first baffle (801) and the second baffle (802); The two ends of the first rotating shaft (807) are respectively fixedly supported on one side of the equipment frame (1) in front of the beater chamber (4) by a first support rod (809); The two ends of the fixed rod (808) are respectively fixedly supported on the other side of the equipment frame (1) in front of the beat chamber (4) by a second support rod (810).

4. The apparatus for digesting adherent cells in a sheet-like carrier cell culture bag according to claim 1, characterized in that: The swing bed (9) includes a swing bed tray (901). A manual clamping element (902) is provided on the front and rear sides or left and right sides of the upper part of the swing bed tray (901). A swing bed top plate (903) is provided at the bottom of the swing bed tray (901). A swing bed cover (904) is provided below the swing bed top plate (903). The swing bed cover (904) is movably supported on the equipment frame (1) at the top of the electrical control compartment (2). Two swing bed support seats (905) are provided inside the swing bed cover (904). The upper parts of the two swing bed support seats (905) penetrate the swing bed cover (904) and are respectively hinged to the bottom of the swing bed top plate (903) via a second pivot (906). The outer cover (904) of the swing bed is also equipped with a swing bed motor (907), a swing arm (908), a swing bed base plate (909) and four weighing modules (910). The swing bed motor (907) is supported on the inner side of one of the swing bed support seats (905) and connected to the industrial control computer. The swing arm (908) is located on the outer side of the swing bed support seat (905) that supports the swing bed motor (907), and one end is connected to the output end of the swing bed motor (907) and the other end is connected to the top plate (903) of the swing bed. The swing bed base plate (909) is fixed at the bottom of the two swing bed support seats (905). The four weighing modules (910) are located at the four corners of the bottom of the swing bed base plate (909) and are all connected to the industrial control computer.

5. The apparatus for digesting adherent cells in a sheet-like carrier cell culture bag according to claim 1, characterized in that: The temperature control box (10) includes a temperature control box body (1001), a box cover (1002) is provided on the top of the temperature control box body (1001), a tray (1003) is provided inside the temperature control box body (1001), three nylon plates (1004) are provided on the upper part of the tray (1003), a heating plate (1005) is provided on the upper part of each nylon plate (1004), a temperature sensor (1006) is embedded in the middle of each heating plate (1005), and a placement area for placing disposable culture medium bags (100) is provided on the upper part of each heating plate (1005). The heating plate (1005) and the temperature sensor (1006) are both electrically connected to the industrial control computer.

6. The apparatus for digesting adherent cells in a sheet-like carrier cell culture bag according to claim 5, characterized in that: A first clamp valve (12), a second clamp valve (13), and a third clamp valve (14) are arranged sequentially from left to right on the front side of the temperature control box (1001). A first hose (19) is provided on the first clamp valve (12), a second hose (20) is provided on the second clamp valve (13), and a third hose (21) is provided on the third clamp valve (14). One end of the first hose (19), the second hose (20), and the third hose (21) Each of the three disposable culture medium bags (100) is connected to one of the three heating plates (1005) respectively, and the other end of each bag is connected to the liquid inlet tube (22). The liquid inlet tube (22) is supported on the first peristaltic pump (15) and is used to connect to the liquid inlet of the disposable sheet-like carrier cell culture bag (200) located in front of the tapping chamber (4). The first peristaltic pump (15) is located in front of the equipment frame (1) and is connected to the industrial control computer.

7. The apparatus for digesting adherent cells in a sheet-like carrier cell culture bag according to claim 4, characterized in that: A fifth clamp valve (18), a fourth clamp valve (17), and a second peristaltic pump (16) are arranged sequentially from left to right on the front side of the swing bed cover (904). A fifth hose (25) is provided on the fifth clamp valve (18), a fourth hose (24) is provided on the fourth clamp valve (17), and a liquid outlet hose (23) is provided on the second peristaltic pump (16). One end of the outlet tube (23) is connected to the outlet of the disposable sheet-like carrier cell culture bag (200) located in front of the tapping chamber (4), and the other end is connected to the fourth tube (24) and the fifth tube (25) respectively. The other end of the fourth tube (24) is connected to the disposable cell finished product bag (300) placed on the swing bed (9), and the other end of the fifth tube (25) is connected to the disposable waste bag (400) placed in the waste liquid drawer (5). The second peristaltic pump (16) is connected to the industrial control computer.

8. The apparatus for digesting adherent cells in a sheet-like carrier cell culture bag according to claim 6, characterized in that: The three disposable culture medium bags (100) placed on the three heating plates (1005) are, from left to right, a disposable culture medium bag (100) containing phosphate buffer solution, a disposable culture medium bag (100) containing trypsin, and a disposable culture medium bag (100) containing serum-containing cell growth medium.

9. The apparatus for digesting adherent cells in a sheet-like carrier cell culture bag according to claim 1, characterized in that: A caster wheel (26) is provided at each of the four corners of the bottom of the equipment frame (1).

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