Cell culture device and cell culture method

Abstract

The application discloses a cell culture device and a cell culture method. The device comprises a culture dish assembly, an adjusting cover and an adjusting assembly. The culture dish assembly comprises a box body and a partition plate assembly. In use, cells can be cultured in multiple culture spaces formed by the partition plate assembly and the box body. Further, culture solution can be injected into the culture spaces for cell culture. The adjusting cover and the adjusting assembly are arranged so that the cell culture device can have different culture modes. Therefore, one cell culture device can be used to culture cells under different culture conditions, the diversity of cell culture is improved, and comparison tests can be performed by using one cell culture device, so that the optimal cell culture scheme can be determined.

Description

Technical Field

[0001] This application relates to the field of enzymology or microbiology devices, and more particularly to a cell culture device and a cell culture method. Background Technology

[0002] Cell culture refers to a method of simulating the in vivo environment (sterile, suitable temperature, pH, and certain nutritional conditions) in vitro to enable cells to survive, grow, reproduce, and maintain their main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technique. Cell culture is an indispensable process for both bioengineering as a whole and its specific application in cloning; cell culture itself is the large-scale cloning of cells. Cell culture technology can cultivate a single cell into a large number of simple single cells or minimally differentiated multicellular organisms, which is an essential step in cloning technology, and cell culture itself is cell cloning. Cell culture technology is an important and commonly used technique in cell biology research. Through cell culture, large numbers of cells can be obtained, and these cells can be used to study cell signal transduction, cellular synthesis and metabolism, cell growth and proliferation, etc. However, traditional cell culture devices have limited structure and function, allowing only single-mode cell culture, which is not conducive to comparative experiments. Summary of the Invention

[0003] The present invention aims to solve at least one of the technical problems existing in the prior art or related art.

[0004] Therefore, a first aspect of the present invention provides a cell culture apparatus.

[0005] A second aspect of the present invention provides a method for cell culture.

[0006] In view of the above, a cell culture apparatus is provided according to a first aspect of the embodiments of this application, comprising: A culture dish assembly, comprising a box body and a partition plate assembly, wherein the partition plate assembly is disposed on the box body to divide the box body into multiple culture spaces, and the partition plate assembly has multiple through holes for the flow of culture medium; An adjustment cover, wherein the culture dish assembly is disposed within the adjustment cover; An adjustment component is provided between the adjustment cover and the culture dish assembly, and the adjustment component is used to adjust the tilt angle between the first bottom plate of the box and the second bottom plate of the adjustment cover.

[0007] In one feasible implementation, the regulating component includes: The first connecting rod has one end hinged to the adjusting cover and the other end provided with a ball head, which is connected to the middle of the first base plate; Multiple telescopic rods are arranged around the first connecting rod, with one end of each telescopic rod connected to the body adjustment cover and the other end connected to the box body.

[0008] In one feasible implementation, the regulating component includes: The second link has one end hinged to the adjustment cover and the other end provided with a ball head, which is connected to the middle of the first base plate; A capsule, the capsule being arranged around the periphery of the second connecting rod, the capsule including a plurality of spacer membranes to divide the capsule into a plurality of media storage spaces; A media injection unit, connected to multiple media storage spaces, is used to inject media into the media storage spaces; The medium may be a gas or a liquid.

[0009] In one possible implementation, a slot is formed on the box body, and the partition assembly is recessed into the slot.

[0010] In one feasible implementation, the partition assembly is placed inside the box, and both the outer contour of the partition assembly and the inner contour of the box are circular, and the partition assembly is rotatable relative to the box.

[0011] In one possible implementation, the partition assembly includes a plurality of partitions arranged in a cross configuration.

[0012] In one feasible implementation, the geometry of the partition assembly projected onto the bottom plate of the box includes a cross portion and a broken line portion connecting the endpoints of each cross portion, the cross portion and the broken line portion together dividing the box into multiple culture spaces.

[0013] In one feasible implementation, the cell culture apparatus further includes: Multiple sub-heating elements are provided. The adjustment cover includes a side plate and a second bottom plate. The side plate is connected to the second bottom plate and wraps around the side wall of the box body. The multiple sub-heating elements are evenly distributed on the side plate and the second bottom plate. A control unit, connected to multiple sub-heating elements, is used to control the opening and closing of each sub-heating element; A temperature sensor, arranged on the box body, is used to detect the temperature in different culture spaces within the box body, and the temperature sensor is communicatively connected to the control unit. A cover plate, the cover plate being provided on the adjusting cover.

[0014] A cell culture method is provided according to a second aspect of the embodiments of this application, applied to a cell culture apparatus as described in any of the above technical solutions, the cell culture method comprising: In response to the balanced cultivation instruction information, the setting position of the box is adjusted by the adjustment component so that the first bottom plate is parallel to the second bottom plate; In response to the differentiated cultivation instruction information, the setting position of the box is adjusted by the adjustment component so that the first base plate is tilted relative to the second base plate; In response to interactive cultivation instructions, the setting position of the box is adjusted by the adjustment component so that the tilt of the first base plate changes periodically.

[0015] In one feasible implementation, the cell culture method further includes: In response to differentiated culture command information, the operating state of multiple sub-heating elements is controlled so that at least some of the culture spaces in the multiple culture spaces have temperature differences.

[0016] Compared with the prior art, the present invention has at least the following beneficial effects: The cell culture apparatus provided in this application includes a culture dish assembly, an adjustment cover, and an adjustment component. The culture dish assembly includes a box and a partition plate assembly. During use, cells can be cultured in multiple culture spaces formed by the partition plate assembly and the box. Furthermore, culture medium can be perfused into the culture spaces for cell culture. Through the adjustment cover and adjustment component, the cell culture apparatus can accommodate different culture methods, allowing a single apparatus to culture cells under different conditions, thus increasing the diversity of cell culture. Comparative experiments can be conducted using a single cell culture apparatus, facilitating the identification of the optimal cell culture protocol. Simultaneously, multiple vias formed on the partition plate assembly allow for cell signal communication, facilitating research on signal transduction between cells. For example, different cells can be cultured in different culture spaces. Therefore, the cell culture apparatus provided in this application enriches cell culture methods and facilitates research on signal transduction between cells. Attached Figure Description

[0017] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of this application. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings: Figure 1 A schematic structural diagram of a cell culture apparatus in one working state according to an embodiment of this application; Figure 2 A schematic structural diagram of another working state of the cell culture apparatus provided in one embodiment of this application; Figure 3 A schematic structural diagram of a cell culture apparatus according to one embodiment of this application from another angle; Figure 4 A schematic structural diagram of a cell culture apparatus according to another embodiment of this application; Figure 5 A schematic flowchart illustrating the steps of a cell culture method according to another embodiment of this application.

[0018] in, Figures 1 to 4 The correspondence between the reference numerals and component names in the attached drawings is as follows: 1. Box body, 2. Divider plate assembly, 3. Adjustment cover, 4. First connecting rod, 5. Telescopic rod, 6. Second connecting rod, 7. Bag body, 8. Sub-heating element; 201 Cross section, 202 Broken line section. Detailed Implementation

[0019] To better understand the above technical solutions, the technical solutions of the embodiments of this application will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the embodiments of this application and the specific features in the embodiments are detailed descriptions of the technical solutions of the embodiments of this application, rather than limitations on the technical solutions of this application. In the absence of conflict, the embodiments of this application and the technical features in the embodiments can be combined with each other.

[0020] like Figures 1 to 4 As shown, a cell culture apparatus is provided according to a first aspect of the embodiments of this application, comprising: a culture dish assembly, the culture dish assembly including a box body 1 and a partition plate assembly 2, the partition plate assembly 2 being disposed on the box body 1 to divide the box body 1 into multiple culture spaces, the partition plate assembly 2 having multiple through holes for the flow of culture medium; an adjustment cover 3, the culture dish assembly being disposed inside the adjustment cover 3; and an adjustment component, the adjustment component being disposed between the adjustment cover 3 and the culture dish assembly, the adjustment component being used to adjust the tilt angle between the first bottom plate of the box body 1 and the second bottom plate of the adjustment cover 3.

[0021] The cell culture device provided in this application includes a culture dish assembly, an adjustment cover 3, and an adjustment component. The culture dish assembly includes a box body 1 and a partition plate assembly 2. During use, cells can be cultured in multiple culture spaces formed by the partition plate assembly 2 and the box body 1. Furthermore, culture medium can be injected into the culture spaces for cell culture. By setting the adjustment cover 3 and the adjustment component, the first bottom plate of the box body 1 can be tilted. Through the influence of gravity on the culture medium, the content of culture medium in different culture spaces can be different (more nutrient medium in the low-lying areas). This allows the cell culture device to have different culture methods, enabling a single cell culture device to culture cells under different culture conditions, thus improving the diversity of cell culture. Comparative experiments can be conducted using a single cell culture device, making it easier to determine the optimal cell culture scheme.

[0022] The cell culture apparatus provided in this application embodiment allows cells to communicate signals through multiple vias formed on the isolation plate assembly, facilitating research on signal transduction between cells. For example, different cells can be cultured in different culture spaces. Based on this, the cell culture apparatus provided in this application embodiment can enrich cell culture methods and facilitate research on signal transduction between cells.

[0023] It is understood that, through the cell culture device provided in the embodiments of this application, by periodically adjusting the tilt angle of the box 1 by controlling the adjustment component, the culture medium can circulate between multiple culture spaces, which facilitates the absorption of nutrients during cell culture.

[0024] like Figure 1 and Figure 2 As shown, in one feasible embodiment, the adjustment assembly includes: a first connecting rod 4, one end of which is hinged to the adjustment cover 3, and the other end is provided with a ball head, which is connected to the middle of the first base plate; and a plurality of telescopic rods 5, which are arranged around the first connecting rod 4, one end of which is connected to the adjustment cover 3 on the body, and the other end of which is connected to the box body 1.

[0025] In this technical solution, the structural composition of the adjustment component is further provided. The adjustment component may include a first connecting rod 4 and multiple telescopic rods 5. The first connecting rod 4 serves to fix the box body 1. By controlling the extension and retraction of different telescopic rods 5, the tilt angle of the first bottom surface of the box body 1 can be adjusted, thereby controlling the flow of culture medium in multiple culture spaces. At the same time, the culture medium content in some culture spaces within the box body 1 can be higher, and the culture medium content in some culture spaces can be lower. This allows a single cell culture device to provide different culture environments, facilitating comparative experiments and identifying the optimal cell culture scheme. Furthermore, by periodically changing the extension and retraction of the telescopic rods 5, the culture medium can flow between multiple culture spaces, facilitating the acquisition of nutrients for cell culture.

[0026] like Figure 4 As shown, in one feasible embodiment, the adjustment assembly includes: a second connecting rod 6, one end of which is hinged to the adjustment cover 3, and the other end is provided with a ball head connected to the middle of the first base plate; a capsule 7, which is arranged around the second connecting rod 6, and includes multiple spacer membranes inside the capsule 7 to divide the capsule 7 into multiple media storage spaces; and a media injection section, which is connected to the multiple media storage spaces and is used to inject media into the media storage spaces; wherein the media includes gas or liquid.

[0027] In this technical solution, the adjustment component may include a second connecting rod 6, a capsule 7, and a medium injection section. By injecting medium into different medium storage spaces through the medium injection section, the thickness of each medium injection section can be adjusted, thereby adjusting the tilt angle of the first bottom plate of the box 1. This allows for higher culture medium content in some culture spaces and lower culture medium content in others within the box 1, enabling the provision of different culture environments through a single cell culture device. This facilitates comparative experiments and helps determine the optimal cell culture scheme. Simultaneously, by periodically changing the extension and retraction of the telescopic rod 5, the culture medium can flow between multiple culture spaces, facilitating the acquisition of nutrients for cell culture.

[0028] In one feasible implementation, a slot is formed on the box body 1, and the partition plate assembly 2 is recessed into the slot.

[0029] In this technical solution, a slot can be formed on the box body 1, and the partition plate assembly 2 is set in the slot by sinking, so that the fixation of the partition plate assembly 2 is more reliable.

[0030] In one feasible implementation, the partition assembly 2 is placed inside the box body 1, and both the outer contour of the partition assembly 2 and the inner contour of the box body 1 are circular, and the partition assembly 2 is rotatable relative to the box body 1.

[0031] In this technical solution, the partition plate assembly 2 can also be placed inside the box body 1. The outer contour of the partition plate assembly 2 and the inner hub are adapted to each other, so that the partition plate assembly 2 can rotate relative to the box body 1. The rotation of the partition plate assembly 2 facilitates the flow of culture medium in different culture spaces and promotes the absorption of nutrients by cells.

[0032] In one feasible implementation, the partition assembly 2 includes a plurality of partitions arranged in a cross configuration. This arrangement facilitates the assembly and molding of the partition assembly 2.

[0033] like Figure 3As shown, in one feasible embodiment, the geometry of the orthographic projection of the partition plate assembly 2 onto the bottom plate of the box body 1 includes a cross portion 201 and a broken line portion 202 connecting the endpoints of each cross portion 201. The cross portion 201 and the broken line portion 202 together divide the box body 1 into multiple culture spaces.

[0034] In this technical solution, a further style of the partition plate assembly 2 is provided. The geometric shape of the partition plate assembly 2 projected onto the bottom plate of the box body 1 includes a cross portion 201 and a broken line portion 202 connecting the endpoints of each cross portion 201. The cross portion 201 and the broken line portion 202 together divide the box body 1 into multiple culture spaces. In this way, the cross portion 201 and the broken line portion 202 can divide one box body 1 into eight culture spaces. The cross portion 201 and the broken line portion 202 can limit the volume of each culture space and the size of the wall of each culture space. When the box body 1 is tilted, the flow rate of the culture medium can be controlled, thereby avoiding the culture medium from flowing too fast and affecting the cell culture.

[0035] In one feasible embodiment, the cell culture apparatus further includes: a plurality of sub-heating elements 8, an adjustment cover 3 including a side plate and a second bottom plate, the side plate being connected to the second bottom plate and wrapping the side wall of the box body 1, the plurality of sub-heating elements 8 being evenly distributed on the side plate and the second bottom plate; a control element connected to the plurality of sub-heating elements 8 for controlling the opening and closing of each sub-heating element 8; a temperature sensor arranged on the box body 1 for detecting the temperature in different culture spaces of the box body 1, the temperature sensor being communicatively connected to the control element; and a cover plate disposed on the adjustment cover 3.

[0036] In this technical solution, the cell culture device may also include multiple sub-heating elements 8, control elements, and cover plates. By setting multiple sub-heating elements 8, control elements, and cover plates, the temperature of different culture spaces can be controlled separately, so that cells can be cultured in different culture spaces at different temperatures. Based on this, combined with the inclination angle of the first bottom surface of the box 1, the cell culture device provided in this application can conduct comparative experiments on cell culture settings from two perspectives: temperature and nutrient solution content, which can make the cell culture device more diversified in terms of cell culture methods.

[0037] like Figures 1 to 5 As shown, a cell culture method is provided according to a second aspect of the embodiments of this application, applied to a cell culture apparatus as described in any of the above technical solutions. The cell culture method includes: Step 211: In response to the balanced culture command information, adjust the setting position of the box body by adjusting the component so that the first base plate is parallel to the second base plate. During use, the adjusting cover 3 can be set inside the test bench or culture equipment. The second base plate of the adjusting cover 3 is set horizontally. By adjusting the setting position of the box body 1 by adjusting the component, the first base plate is made parallel to the second base plate. This allows the first base plate to be set horizontally, thereby ensuring that the culture medium is evenly distributed in different culture spaces. The culture state in all culture spaces is consistent, thus achieving balanced culture.

[0038] Step 212: In response to the differential culture instruction information, adjust the setting position of the box body by adjusting the component so that the first base plate is tilted relative to the second base plate. When differential culture is required, the setting position of the box body 1 can be adjusted by adjusting the component so that the first base plate is tilted relative to the second base plate. Based on this, some culture spaces in multiple culture spaces can contain more culture medium, while others contain less culture medium, enabling differential cell culture.

[0039] Step 213: In response to the interactive culture command information, the setting position of the housing 1 is adjusted by the adjustment component to make the tilt of the first base plate change periodically. In this technical solution, in order to realize the information transmission between cells and promote the flow of culture medium, the tilt direction of the first base plate can be changed periodically.

[0040] The cell culture method provided in this application embodiment is applied to the cell culture device of any of the above technical solutions, and therefore the cell culture method has all the beneficial effects of the cell culture device of the above calculation solution.

[0041] In one feasible implementation, the cell culture method further includes: controlling the operating state of a plurality of sub-heating elements 8 in response to differential culture instruction information, so that at least a portion of the culture spaces in the plurality of culture spaces have a temperature difference.

[0042] In this technical solution, during differential culture, the working state of multiple sub-heating elements 8 can be controlled to create temperature differences in at least some of the culture spaces, thereby enabling differential cell culture based on temperature.

[0043] In this invention, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance; the term "multiple" refers to two or more unless otherwise explicitly defined. The terms "install," "connect," "link," and "fix" should be interpreted broadly. For example, "connect" can be a fixed connection, a detachable connection, or an integral connection; "link" can be a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0044] In the description of this invention, it should be understood that the terms "upper," "lower," "left," "right," "front," "rear," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or unit referred to must have a specific orientation or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0045] In the description of this specification, the terms "one embodiment," "some embodiments," "specific embodiment," etc., refer to a specific feature, structure, material, or characteristic described in connection with that embodiment or example, which is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0046] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A cell culture device, characterized in that, include: A culture dish assembly, comprising a box body and a partition plate assembly, wherein the partition plate assembly is disposed on the box body to divide the box body into multiple culture spaces, and the partition plate assembly has multiple through holes for the flow of culture medium; An adjustment cover, wherein the culture dish assembly is disposed within the adjustment cover; An adjustment component is disposed between the adjustment cover and the culture dish assembly. The adjustment component is used to adjust the tilt angle between the first bottom plate of the box and the second bottom plate of the adjustment cover, so as to make the culture medium content different in different culture spaces by means of the influence of gravity on the culture medium. The partition assembly is placed inside the box, and both the outer contour of the partition assembly and the inner contour of the box are circular. The partition assembly is rotatable relative to the box. The partition assembly includes a plurality of partitions arranged in a cross configuration; The geometry of the partition assembly projected onto the bottom plate of the box includes a cross section and a broken line section connecting the endpoints of each cross section, the cross section and the broken line section together dividing the box into multiple culture spaces. The volume of each culture space and the dimensions of the wall of each culture space are defined by the cross section and the zigzag section, and the flow rate of the culture medium is controlled when the box is tilted. Cell culture methods applied to cell culture devices include: In response to the balanced cultivation instruction information, the setting position of the box is adjusted by the adjustment component so that the first bottom plate is parallel to the second bottom plate; In response to the differentiated cultivation instruction information, the setting position of the box is adjusted by the adjustment component so that the first base plate is tilted relative to the second base plate; In response to interactive cultivation instructions, the setting position of the box is adjusted by the adjustment component so that the tilt of the first base plate changes periodically.

2. The cell culture apparatus according to claim 1, characterized in that, The adjustment component includes: The first connecting rod has one end hinged to the adjusting cover and the other end provided with a ball head, which is connected to the middle of the first base plate; Multiple telescopic rods are arranged around the first connecting rod, with one end of each telescopic rod connected to the body adjustment cover and the other end connected to the box body.

3. The cell culture apparatus according to claim 1, characterized in that, The adjustment component includes: The second link has one end hinged to the adjustment cover and the other end provided with a ball head, which is connected to the middle of the first base plate; A capsule, the capsule being arranged around the periphery of the second connecting rod, the capsule including a plurality of spacer membranes to divide the capsule into a plurality of media storage spaces; A media injection unit, connected to multiple media storage spaces, is used to inject media into the media storage spaces; The medium may be a gas or a liquid.

4. The cell culture apparatus according to claim 1, characterized in that, The box body has a slot, and the partition plate assembly is recessed into the slot.

5. The cell culture apparatus according to any one of claims 1 to 4, characterized in that, Also includes: Multiple sub-heating elements are provided. The adjustment cover includes a side plate and a second bottom plate. The side plate is connected to the second bottom plate and wraps around the side wall of the box body. The multiple sub-heating elements are evenly distributed on the side plate and the second bottom plate. A control unit, connected to multiple sub-heating elements, is used to control the opening and closing of each sub-heating element; A temperature sensor, arranged on the box body, is used to detect the temperature in different culture spaces within the box body, and the temperature sensor is communicatively connected to the control unit. A cover plate, which is used to cover the adjusting cover.

6. The cell culture apparatus according to claim 1, characterized in that, Cell culture methods also include: In response to differentiated culture command information, the operating state of multiple sub-heating elements is controlled so that at least some of the culture spaces in the multiple culture spaces have temperature differences.

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