菌株及其在制备泰乐菌素A中的应用

By overexpressing acetyl-CoA carboxylase and phosphopanylthioethylamine transferase genes in *Streptomyces freundii*, a recombinant engineered strain WT/FGIH was constructed, solving the problem of increasing tylosin production and achieving a nearly two-fold increase in tylosin yield while reducing production costs.

CN116426450BActive Publication Date: 2026-07-17INST OF MICROBIOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF MICROBIOLOGY CHINESE ACAD OF SCI
Filing Date
2023-04-01
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies make it difficult to further increase the yield of tylosin through traditional methods, resulting in high production costs and insufficient competitiveness.

Method used

Using genetic engineering techniques, a recombinant engineered strain WT/FGIH was constructed by using a strong promoter to drive the high expression of the acetyl-CoA carboxylase gene and the phosphoproteopancreatoyl thioethylamine transferase gene. The recombinant plasmid was then introduced into Streptomyces freundii using an Escherichia coli-Streptomyces shuttle plasmid.

Benefits of technology

It increased the yield of tylosin by nearly 2 times, reduced production costs, and enhanced the competitiveness of production enterprises.

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Abstract

本发明涉及基因工程和微生物技术领域。本发明公开一种基因在构建产泰乐菌素A的链霉菌中的应用，所述基因包括：强启动子和ovmFGIH基因。本发明的工程菌株制备泰乐菌素A的产量比出发菌株提高了2倍。
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