一种dsRNA对叶螨直接毒性影响的测定方法
By soaking spider mite eggs in a mixture of dsRNA and Tween solution, and then using RT-qPCR to detect changes in the expression levels of target genes in spider mites, the problem of insufficient effectiveness and sensitivity of dsRNA in the determination of spider mite RNAi in existing methods is solved, and a simple and effective determination method is provided.
CN116426646BActive Publication Date: 2026-07-17SHANXI AGRI UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHANXI AGRI UNIV
- Filing Date
- 2023-02-28
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing methods for measuring dsRNA against spider mite RNAi lack sufficient effectiveness and sensitivity, making it difficult to effectively assess its direct toxic effects on spider mites.
Method used
The toxic effects of dsRNA were evaluated by soaking spider mite eggs in a mixture of dsRNA and Tween solution, followed by RT-qPCR detection of changes in the expression levels of target genes in spider mites and observation of biological changes.
Benefits of technology
This study enables the effective determination of the direct toxic effects of dsRNA on spider mites. The method is simple and feasible, improving the effectiveness and sensitivity of the assay.
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Abstract
本发明公开了一种dsRNA对叶螨直接毒性影响的测定方法，属于农业生物技术领域；包括将针对相关靶基因的dsRNA与吐温溶液混合后浸泡叶螨卵，以使dsRNA导入到叶螨体内，并测定外源dsRNA对叶螨的毒性；该方法用以研究基因功能和评价外源dsRNA对叶螨的毒性；该方法能够有效测定外源dsRNA对叶螨的直接毒性影响，且方法简单可行，有效性和灵敏性好，对相关基因功能的研究和RNAi靶基因筛选具有重要的意义和应用前景。
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