一种催化活性提高的12α-HSDH酶突变体及其用途
By performing site-directed mutagenesis on 12α-HSDH enzyme to improve its catalytic activity, the problems of insufficient enzyme activity and environmental pollution were solved, and efficient production of 12-ketochenodeoxycholic acid was achieved, which is suitable for industrial ursodeoxycholic acid production.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHONGSHAN BAILING BIOTECHNOLOGY CO LTD
- Filing Date
- 2022-12-21
- Publication Date
- 2026-07-17
AI Technical Summary
The current 12α-HSDH enzyme activity is insufficient, resulting in low production efficiency of ursodeoxycholic acid. Furthermore, the traditional chemical method causes serious pollution from oxidants, which does not meet the requirements of sustainable development.
By performing protein structure simulation, site-directed saturation mutagenesis, and Rosetta-assisted rational design on the 12α-HSDH enzyme derived from Clostridium sp. AF15-17LB, a 12α-HSDH mutant with enhanced catalytic activity was constructed by mutating serine at position 101 to alanine.
The mutant enzyme activity is increased by 1.5 times, and the substrate conversion rate and production rate are significantly improved, making it suitable for industrial production of 12-ketochenodeoxycholic acid, reducing production costs and environmental pollution.