即用型DAB显色液及其制备方法

By optimizing the formulation of DAB colorimetric solution and combining components such as DAB, dextran-10, 1,2-propanediol, imidazole, surfactant, and hydrogen peroxide, a ready-to-use DAB colorimetric solution was prepared, solving the problems of inconvenience and pollution associated with traditional colorimetric reagents and improving stability and convenience.

CN116448992BActive Publication Date: 2026-07-17HANGZHOU BIOLYNX TECH CO LTD

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HANGZHOU BIOLYNX TECH CO LTD
Filing Date
2023-04-13
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Traditional DAB colorimetric reagents need to be stored and prepared separately, which is inconvenient to use and easily pollutes the environment. Furthermore, the prepared reagents cannot be stored for a long time, affecting detection efficiency and stability.

Method used

A formula for a ready-to-use DAB colorimetric solution is provided, comprising components such as DAB, dextran-10, 1,2-propanediol, imidazole, surfactant, preservative, and hydrogen peroxide. The solution is prepared into a stable ready-to-use colorimetric solution by mixing and adjusting the pH value, thus avoiding separate storage and preparation processes.

Benefits of technology

This achieves stability and ease of use of the DAB colorimetric solution, reduces the risk of contact for laboratory personnel, lowers environmental pollution, and shortens the detection time.

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Abstract

本发明涉及一种即用型DAB显色液及其制备方法。所述即用型DAB显色液包含溶剂以及如下各组分:DAB、葡聚糖‑10、1,2‑丙二醇、咪唑、表面活性剂、防腐剂和过氧化氢;或者,所述即用型DAB显色液还包含山梨糖醇、EDTA‑2Na、D‑葡萄糖酸钠、焦磷酸钠和硫酸镁中的一种或者多种。本申请通过配方优化,提供一种特定配方的DAB显色液,该DAB显色液配方属于DAB色原与DAB底物缓冲液的一体化配方且稳定性好,无需配制即能够直接用于免疫组化检测,有效避免技术人员与DAB的接触,降低环境污染,缩短检测耗时。
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