Preparation method of cross-linked full-biomass luminescent hydrogel material based on puffed feather powder

Abstract

The application discloses a preparation method of a crosslinked full-biomass luminescent hydrogel material based on expanded feather meal, and first uses expanded feather meal PFM as a luminescent raw material and a crosslinking agent to prepare the full-biomass luminescent hydrogel material which is degradable and biocompatible, and the process is simple, the waste resources are reused, and the cost is reduced and the ecological environment is protected.

Description

Technical fields:

[0001] This invention relates to the field of biomass-based luminescent hydrogel material preparation technology, specifically to a method for preparing a biomass-based luminescent hydrogel material based on puffed feather powder crosslinking. Background technology:

[0002] The phenomenon of bioluminescence has long fascinated scientists. While seemingly magical, it is actually quite common in nature. Based on differences in their luminescence mechanisms, bioluminescence can be divided into bioluminescence and stimulated fluorescence. Our understanding of bioluminescence has progressed from initially using luminescent organisms as lighting tools to exploring its mechanisms and developing various fluorescent proteins; scientific advancements have been continuous. Especially in recent years, experts both domestically and internationally have used bioluminescent biomass as a building block, leveraging its unique chemical structure to synthesize a large number of bioluminescent materials in situ. Biomass-based fluorescent materials have wide applications in food detection, bioimaging, encrypted printing, light-emitting devices, and thermodynamic energy conversion.

[0003] Bioluminescent organisms are found in almost every region of the Earth, with 80% being marine life. While the distribution of bioluminescence in non-marine organisms is narrower, the range of colors they emit is much richer. Feathers, as a natural biomass rich in keratin, possess excellent biocompatibility, biodegradability, renewability, film-forming properties, and endogenous luminescence. Therefore, the development and utilization of waste feathers has received widespread attention. Extruded feather powder (PFM), a material obtained by shearing feathers under high temperature and pressure, has advantages such as biodegradability, low cost, and environmental friendliness, making it a material with high development value. Summary of the Invention:

[0004] The purpose of this invention is to provide a method for preparing a biomass luminescent hydrogel material based on puffed feather powder crosslinking. For the first time, puffed feather powder (PFM) is used as a luminescent raw material and crosslinking agent to prepare a biodegradable and biocompatible biomass luminescent hydrogel material. The process is simple, realizes the reuse of waste resources, reduces costs and protects the ecological environment.

[0005] This invention is achieved through the following technical solutions:

[0006] A method for preparing a biodegradable biomass luminescent hydrogel material based on puffed feather powder crosslinking includes the following steps: dispersing puffed feather powder and one of the following: seaweed powder, macadamia nut shell, sugarcane bagasse, cellulose nanofibers, chitosan, small protein molecules or fluorescent small molecules in deionized water, and then heating it in a high-temperature sterilizer at 118-125℃ for 25-35 minutes to synthesize a biodegradable biomass luminescent hydrogel material in one step.

[0007] Preferably, the mass ratio of puffed feather powder to deionized water is (0.1-0.3):1.

[0008] The mass ratio of seaweed powder, macadamia nut shell, sugarcane bagasse, cellulose nanofibers, chitosan, small protein molecules or fluorescent small molecules to puffed feather powder is (0.5-4):(4-10).

[0009] Preferably, the puffed feather powder is either puffed duck feather powder or puffed chicken feather powder.

[0010] Seaweed powder can be selected from any one of Sargassum powder, nori powder, and kelp powder.

[0011] The preparation method of cellulose nanofibers is as follows: Deionized water is added to bleached conifer sulfate pulp, unbleached conifer sulfate pulp, bleached eucalyptus sulfate pulp, or straw sulfate pulp. After stirring for 30 minutes (300 rpm), sodium bromide and 0.8 g of 2,2,6,6-tetramethylpiperidine oxide (TEMPO) are added respectively. Then, sodium hypochlorite solution (the amount of oven-dry pulp) is added to the mixture to obtain TOCNFs. During the oxidation process, the pH of the system is controlled at around 10.5 using 0.5 M sodium hydroxide. The reaction is terminated with ethanol. The mixture is washed repeatedly with deionized water three times, and then homogenized using an IKAT25 homogenizer for 3 hours at 10,000 rpm. After homogenization, a clear and transparent system is obtained.

[0012] Preferably, the fluorescent small molecule is selected from either Rhodamine B or pyrene formaldehyde.

[0013] The beneficial effects of this invention are as follows:

[0014] (1) This invention uses puffed feather powder as a luminescent raw material and crosslinking agent. It is abundant, non-toxic and degradable, and can be reused as a waste resource. It prepares a biodegradable and biocompatible porous biomass luminescent hydrogel material, which can be used not only as a luminescent material for biological probes, sensors and environmental monitoring, but also in the fields of solar energy conversion, heavy metal determination and cell imaging.

[0015] (2) The composite luminescent gel material of the present invention is synthesized in one step, which is simple and saves energy. Attached image description:

[0016] Figure 1 SEM images of the luminescent hydrogels in Examples 1-6.

[0017] Figure 2 The pressure-stress curves are for the luminescent hydrogels of Examples 1-6.

[0018] Figure 3 The elastic modulus is the luminescent hydrogel of Examples 1-6.

[0019] Figure 4 The swelling curves are for the luminescent hydrogels in Examples 2-5 and 7.

[0020] Figure 5 The infrared spectra of the luminescent hydrogels in Examples 1-6 are shown.

[0021] Figure 6 The TG spectra of the luminescent hydrogels in Examples 1, 2 and 5 are shown.

[0022] Figure 7 The DTA spectra of the luminescent hydrogels in Examples 1, 2 and 5 are shown.

[0023] Figure 8 The image shows the DSC spectrum of the luminescent hydrogel from Example 1.

[0024] Figure 9 The image shows the DSC spectrum of the luminescent hydrogel in Example 3.

[0025] Figure 10 This is the DSC spectrum of the luminescent hydrogel in Example 5.

[0026] Figure 11 The images show the fluorescence emission spectra of the luminescent hydrogels in Examples 1-3 and 7. Detailed implementation method:

[0027] The following is a further description of the invention, but not a limitation thereof.

[0028] Example 1:

[0029] 1. Pass the PFM through a 100-mesh standard sieve.

[0030] 2. Place 5g of PFM in 25mL of deionized water to form a suspension, and then disperse it by ultrasonication.

[0031] 3. Place the suspension from step 2 in an autoclave and heat at 121°C for 30 minutes to obtain the luminescent hydrogel material, labeled as FK-1.

[0032] Example 2:

[0033] Referring to Example 1, the difference lies in the amount of deionized water.

[0034] 5g of PFM, which passed through a 100-mesh standard sieve, was placed in 50mL of deionized water to form a suspension, which was then ultrasonically dispersed. The suspension was then placed in an autoclave and heated at 121℃ for 30 minutes to obtain a luminescent hydrogel material, labeled FK-2.

[0035] Example 3:

[0036] Referring to Example 2, the difference is that 1g of Sargassum powder was also added.

[0037] 5g of PFM (passed through a 100-mesh standard sieve) and 1g of Sargassum powder were placed in 50mL of deionized water to form a suspension, which was then ultrasonically dispersed. The suspension was then placed in an autoclave and heated at 121℃ for 30 minutes to obtain a luminescent hydrogel material, labeled FK-SP-1.

[0038] Example 4:

[0039] Referring to Example 2, the difference is that 2g of Sargassum powder was also added.

[0040] 5g of PFM (passed through a 100-mesh standard sieve) and 2g of Sargassum powder were placed in 50mL of deionized water to form a suspension, which was then ultrasonically dispersed. The suspension was then placed in an autoclave and heated at 121℃ for 30 minutes to obtain a luminescent hydrogel material, labeled FK-SP-2.

[0041] Example 5:

[0042] Referring to Example 2, the difference is that 1g of chitosan was also added.

[0043] 5g of PFM (passed through a 100-mesh standard sieve) and 1g of chitosan were placed in 50mL of deionized water to form a suspension, which was then ultrasonically dispersed. The suspension was then placed in an autoclave and heated at 121℃ for 30 minutes to obtain a luminescent hydrogel material, labeled FK-CS.

[0044] Example 6:

[0045] Referring to Example 2, the difference is that 0.5g of Rhodamine B (RhB) was also added.

[0046] 5g of PFM and 0.5g of RhB, which passed through a 100-mesh standard sieve, were placed in 50mL of deionized water to form a suspension, which was then ultrasonically dispersed. The suspension was then placed in an autoclave and heated at 121℃ for 30 minutes to obtain a luminescent hydrogel material, labeled FK-RhB.

[0047] Example 7:

[0048] Referring to Example 2, the difference is that the amount of deionized water is different, and 15 mL of cellulose nanofibers (TOCNFs) are added.

[0049] 5.0 g of bleached conifer sulfate pulp was placed in a 1 L beaker, and 500 mL of deionized water was added. The mixture was stirred for 30 minutes (300 rpm), followed by the addition of 5 g of sodium bromide and 0.8 g of TEMPO. Then, 8 mmol / g sodium hypochlorite solution (based on the oven-dry pulp volume) was added to the mixture to obtain TOCNFs. During the oxidation process, the pH of the system was maintained at approximately 10.5 using 0.5 M sodium hydroxide. The reaction was terminated with ethanol, and the mixture was washed three times with deionized water. The homogenized mixture was then processed using an IKAT25 homogenizer for 3 hours at 10,000 rpm. The resulting clear and transparent system was stored at 4°C for later use.

[0050] 5g of PFM (passed through a 100-mesh standard sieve) and 15mL of TOCNFs were mixed and placed in 20mL of deionized water to form a suspension, which was then ultrasonically dispersed. The suspension was then placed in an autoclave and heated at 121℃ for 30 minutes to obtain the composite luminescent hydrogel material, labeled FK-TOCNFs.

[0051] The properties of the hydrogels obtained in Examples 1-7 were tested, and the results are shown in [reference needed]. Figure 1-11 .

[0052] Depend on Figure 1 , 2 As shown in Figures 1 and 3, the difference between the pure expanded feather powder hydrogel materials prepared in Examples 1 and 2 lies in the amount of water added. Experimental results show that the pore size of the gel material increases with the increase of water content. Compared with the pure expanded feather powder gel material, the gel material with added seaweed has a more porous structure, larger pores, and more obvious swelling, but its mechanical properties are relatively poor. Furthermore, the mechanical properties of the material deteriorate further with the continuous increase of seaweed content. After adding chitosan, the material becomes softer and the swelling is more obvious, but the mechanical properties are still worse. After adding Rhodamine B (RhB), the pore structure and swelling do not change significantly, but the mechanical properties are improved.

[0053] While the specific embodiments of the present invention have been described above in conjunction with the accompanying drawings, this is not intended to limit the scope of protection of the present invention. Those skilled in the art should understand that various modifications or variations that can be made by those skilled in the art without creative effort based on the technical solutions of the present invention are still within the scope of protection of the present invention.

Claims

1. A method for preparing a biomass luminescent hydrogel material based on cross-linked puffed feather powder, characterized in that, The method includes the following steps: dispersing component A (expanded feather powder) and component B in deionized water, and then heating them in a high-temperature sterilizer at 121°C for 30 minutes to synthesize a biodegradable biomass luminescent hydrogel material in one step; component B is selected from cellulose nanofibers, chitosan, or fluorescent small molecules; the fluorescent small molecule is Rhodamine B.

2. The preparation method according to claim 1, characterized in that, The mass ratio of puffed feather meal to deionized water is (0.1~0.3):

1.

3. The preparation method according to claim 1, characterized in that, The mass ratio of cellulose nanofibers, chitosan or fluorescent small molecules to puffed feather powder is (0.5~4):(4~10).

4. The preparation method according to claim 1, characterized in that, Extruded feather powder is either extruded duck feather powder or extruded chicken feather powder.

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