一种促进蕙兰种子萌发的西地西菌及其应用

Sildenafil HH6 promotes the germination of Cymbidium faberi seeds and the growth of tissue cultures by lysing phosphorus and secreting IAA, solving the problems of low seed germination rate and slow growth of tissue cultures in existing technologies, thus achieving efficient propagation of wild Cymbidium faberi and protection of endangered orchid species.

CN116463266BActive Publication Date: 2026-07-17HUNAN ACAD OF FORESTRY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUNAN ACAD OF FORESTRY
Filing Date
2023-04-28
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies are insufficient to effectively promote the germination of Cymbidium seeds and the growth of tissue cultures. Furthermore, the existing mycorrhizal fungi have long cultivation cycles and are difficult to control, making in-situ propagation and conservation of orchid plants challenging.

Method used

The Cedecea davisae HH6 strain was used to promote seed germination by phosphate solubilization and IAA secretion, and to enhance plant immunity by upregulating stachyose expression, thereby promoting seed germination and tissue culture growth.

Benefits of technology

Sildenafil HH6 significantly improves the seed germination rate and tissue culture growth rate of Cymbidium faberi, enabling large-scale propagation of wild Cymbidium faberi and contributing to the in-situ conservation and industrial production of endangered orchid species.

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Abstract

本发明公开了一种促进蕙兰种子萌发的西地西菌及其应用,该西地西菌的菌种保藏编号为CCTCC No:M 2023257。本发明西地西菌,不仅可以通过溶磷、分泌IAA促进种子萌发,而且可以通过上调水苏糖的表达,全面增强植株的免疫能力,提高植株的机体抗病力,促进植株对有用物质吸收与利用,进而促进种子的萌发与幼苗不定芽的分化。在实际应用中,本发明的西地西菌菌株,可作为蕙兰种子萌发、蕙兰组培物生长的促进剂,不仅可以促进蕙兰种子的高效萌发,而且可以促进蕙兰组培物的快速生长,因而可以实现蕙兰特别是野生蕙兰的大规模繁殖,对于实现濒危野生兰科物种的原位保育,乃至人工繁育的产业化生产具有重要的现实意义。
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