Veterinary drug residue detection device based on SPR technology

Abstract

The application discloses a kind of based on SPR technology's veterinary drug residue detection equipment, belong to veterinary drug residue detection field, a kind of based on SPR technology's veterinary drug residue detection equipment, including fuselage, light source system is equipped in fuselage, SPR sensor, sample introduction system, optical transmission and detection system.The application is by setting light source, polarizer, lens, prism miniaturization high-resolution light path design, cylindrical integrated sensor design, accurately control the liquid flow of chip surface, improve the efficiency and reaction degree of reaction, realize the real-time monitoring of portable SPR, develop to get high throughput, high sensitivity portable surface plasmon resonance spectrometer, by utilizing novel nanomaterial and antigen antibody design multiple novel specificity sensing chip, non-directional screening is carried out to illegal additive and toxic and harmful substance in food, to possible toxic and harmful substance is detected once pretreatment, improve detection efficiency, save detection time.

Description

Technical Field

[0001] This invention relates to the field of veterinary drug residue detection technology, specifically to a veterinary drug residue detection device based on SPR technology. Background Technology

[0002] With technological advancements and improved living standards, people have placed higher demands on food safety, which has become a major issue concerning both the overall economic development of my country and the safety of people's lives. To address food safety emergencies and strengthen food safety supervision, countries around the world have prioritized the construction of food safety risk monitoring and prevention systems.

[0003] Biosensing detection methods have gradually gained widespread application due to their advantages in detection accuracy, cost, and time. Since the application of surface plasmon resonance (SPR) technology in the field of chemical sensor research, it has gradually become a research hotspot in the international sensor field. Because SPR technology has the characteristics of real-time monitoring of reaction dynamics, no need for labeling biological samples, high sensitivity, and no background interference, it is mainly used to study the interactions between biological macromolecules and determine kinetic constants, achieving significant progress in its application in the biosciences. To date, this technology has yielded considerable results in antibody-antigen reaction research, simulation of cell membrane and drug action mechanisms, DNA-protein interaction analysis, and virus detection research.

[0004] Existing SPR detection devices have poor reaction efficiency and reaction extent, and cannot detect potentially toxic and harmful substances in a single pretreatment. Summary of the Invention

[0005] The purpose of this invention is to provide a veterinary drug residue detection device based on SPR technology to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a veterinary drug residue detection device based on SPR technology, comprising a body, wherein the body is provided with a light source system, an SPR sensor, a sample introduction system, and an optical transmission and detection system.

[0007] Preferably, a rotating base is fixedly connected to the upper part of the body, a rotating shaft is rotatably connected inside the rotating base, a cover plate is fixedly connected to the side wall of the rotating shaft, a mounting block is fixedly connected to the side of the cover plate, the light source system is disposed in the mounting block, and the light source system includes a light coupling device and a light source that illuminates the light coupling device.

[0008] Preferably, the SPR sensor is a Kretschmann prism, and the Kretschmann prism is coated with a gold film. The body has a groove for placing the SPR sensor, and the mounting block on the side of the cover plate is intermittently embedded into the groove.

[0009] Preferably, the optical transmission system is disposed within the mounting block, and the optical transmission system includes a collimator and a polarizer disposed in front of the optical coupling system, and a convex lens disposed behind the optical coupling system, and the optical detection system is a CCD miniature spectrometer detector.

[0010] Preferably, the sample introduction system includes a mounting box, which is fixedly connected to the side wall of the machine body. A liquid delivery pipe and a liquid discharge pipe are fixedly connected inside the machine body. Both the liquid delivery pipe and the liquid discharge pipe are connected to a groove. A turntable is rotatably connected inside the mounting box. A clamping plate for engaging the liquid delivery pipe is fixedly connected to the side wall of the turntable. An arc-shaped block that fits against the liquid delivery pipe is fixedly connected inside the mounting box. Three rollers that alternately abut against the liquid delivery pipe are rotatably connected to the side wall of the turntable.

[0011] Preferably, a rotating rod is coaxially fixedly connected to the turntable, a worm gear is fixedly connected to the end of the rotating rod, a connecting seat is fixedly connected to the side wall of the machine body, a worm gear meshing with the worm gear is rotatably connected to the connecting seat, and a first handle is fixedly connected to the bottom of the worm gear.

[0012] Preferably, two symmetrically arranged support rods are fixedly connected to the side of the machine body, and a base is slidably connected to both support rods. A first motor is fixedly connected to the middle of the base, and a drive shaft is fixedly connected to the output end of the first motor. A hexagonal prism is fixedly connected to the end of the drive shaft. The hexagonal prism is intermittently inserted into the top of a worm gear. A screw is rotatably connected to the upper surface of the machine body. The screw is threaded to the side of the base, and a second handle is fixedly connected to the top of the screw.

[0013] Preferably, a first mounting plate is rotatably connected to the side of the machine body away from the mounting box, a nut is threadedly connected to the first mounting plate, and a threaded hole that mates with the nut is provided on the machine body. A receiving cylinder is fixedly connected to one side of the first mounting plate, and a rotating column is fixedly connected to the other side of the first mounting plate. A rotating hole that mates with the rotating column is provided on the machine body. The end of the drain pipe away from the groove extends into the receiving cylinder, and a discharge pipe is fixedly connected to the bottom of the receiving cylinder. A valve is provided on the discharge pipe.

[0014] Preferably, a bracket is rotatably connected to the machine body on the opposite side of the cover plate, a support block for supporting the bracket is fixedly connected to the upper surface of the machine body, a second mounting plate is fixedly connected to the end of the bracket, a plurality of brush rollers are rotatably connected to the second mounting plate, a pulley is fixedly connected to the top of each of the plurality of brush rollers, a belt is drivingly connected between the plurality of pulleys, a limit post is rotatably connected to the upper surface of the second mounting plate, the limit post is in contact with the belt, a mounting frame is fixedly connected to the upper part of the bracket, a second motor is fixedly connected to the middle part of the mounting frame, an output shaft is fixedly connected to the output end of the second motor, and one of the pulleys is fixedly connected to the bottom of the output shaft.

[0015] Preferably, a first piston cylinder is fixedly connected to the upper part of the mounting bracket, a piston plate is slidably connected inside the first piston cylinder, a protrusion is provided on the side wall of the piston plate, a limiting groove that mates with the protrusion is opened on the inner wall of the first piston cylinder, a cylinder is fixedly connected to the lower surface of the piston plate, a cylindrical cam is fixedly connected to the end of the output shaft away from the pulley, a slider is fixedly connected inside the cylinder, the slider is slidably connected in a groove on the side wall of the cylindrical cam, and an L-shaped frame is fixedly connected to the side of the machine body near the receiving cylinder, and two symmetrically arranged slide rods are slidably connected to the L-shaped frame. The bottom of the two sliding rods is fixedly connected to a circular plate. The drain pipe is fixedly connected to the circular plate. The side wall of the circular plate is intermittently in contact with the side wall of the receiving cylinder. A piston rod is fixedly connected to the middle of the circular plate. A second piston cylinder is fixedly connected to the upper surface of the L-shaped frame. The piston rod is slidably connected inside the second piston cylinder. A first pipe is fixedly connected between the first piston cylinder and the second piston cylinder. A second pipe and a third pipe are fixedly connected to the first piston cylinder and the second piston cylinder, respectively. A one-way valve is provided on both the first pipe and the second pipe. A vent valve is provided on the third pipe.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0017] 1. This invention improves the efficiency and degree of reaction by using a miniaturized high-resolution optical path design with a light source, polarizer, lens, and prism, and an integrated cylindrical sensor design to precisely control the liquid flow on the chip surface, thereby enabling real-time monitoring of portable SPR.

[0018] 2. This invention utilizes novel nanomaterials and antigen-antibody designs to create a variety of novel specific sensor chips for non-directional screening of illegal additives and toxic and harmful substances in food. This allows for the detection of potential toxic and harmful substances with a single pretreatment, improving detection efficiency and saving detection time. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of the present invention. Figure 1 ;

[0020] Figure 2 This is a schematic diagram of the overall structure of the present invention. Figure 2 ;

[0021] Figure 3 This is a schematic diagram of the structure of the groove in this invention;

[0022] Figure 4 This is a schematic diagram of the structure of the mounting box of the present invention;

[0023] Figure 5 This is a schematic diagram of the structure of the turntable in this invention;

[0024] Figure 6 This is a schematic diagram of the structure at the base of the present invention;

[0025] Figure 7 This is a schematic diagram of the structure at the hexagonal prism of the present invention;

[0026] Figure 8 This is a schematic diagram of the structure of the brush roller in this invention;

[0027] Figure 9 This is a schematic diagram of the cylindrical cam structure of the present invention;

[0028] Figure 10 This is a schematic diagram of the structure of the second piston cylinder of the present invention.

[0029] In the diagram: 1. Body; 101. Liquid delivery pipe; 102. Liquid drain pipe; 11. Cover plate; 12. Mounting block; 13. Rotating shaft; 14. Rotary seat; 15. Groove; 16. SPR sensor; 17. Support block; 18. Connecting seat; 2. Mounting box; 21. Arc-shaped block; 22. Turntable; 23. Clamping plate; 24. Roller; 25. Rotating rod; 26. Worm gear; 27. Worm; 28. First handle; 3. Base; 31. Support rod; 32. Screw; 33. Second handle; 34. First motor; 35. Drive shaft; 36. Hexagonal prism; 4. L-shaped frame; 41. Slide rod; 42. Circular plate; 43. Piston rod; 44. Second piston cylinder; 441. Third pipe; 442. Vent valve; 45. Receiving cylinder; 451. Discharge pipe; 452. Valve; 46. First mounting plate; 47. Rotary column; 48. Nut; 5. Bracket; 51. Second mounting plate; 52. Brush roller; 53. Pulley; 54. Belt; 55. Limiting post; 56. Second motor; 57. Output shaft; 58. Mounting bracket; 6. Cylindrical cam; 61. Cylinder; 62. Slider; 63. Piston plate; 64. Protrusion; 65. First piston cylinder; 66. First pipe; 67. Second pipe; 68. Check valve. Detailed Implementation

[0030] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0031] Example 1

[0032] Reference Figure 1 and Figure 2 The present invention provides a technical solution: a veterinary drug residue detection device based on SPR technology, including a body 1, which contains a light source system, an SPR sensor 16, a sample introduction system, and an optical transmission and detection system.

[0033] The machine body 1 is connected to a computer. The sample to be tested (such as milk) is pumped into the machine body 1 through the sample introduction system. The light source system illuminates the sample flowing on the SPR sensor 16 after rotating through the optical transmission system. The reflected light is received by the detection system. The detection system draws a curve through the computer. By observing the curve, it can be determined whether there are pesticide or veterinary drug residues.

[0034] A rotating base 14 is fixedly connected to the upper part of the body 1. A rotating shaft 13 is rotatably connected inside the rotating base 14. A cover plate 11 is fixedly connected to the side wall of the rotating shaft 13. A mounting block 12 is fixedly connected to the side of the cover plate 11. The light source system is set inside the mounting block 12, and the light source system includes a light coupling device and a light source that illuminates the light coupling device.

[0035] The light source system is used to emit the light required for detection. The optical coupling device can be a grating, prism, optical fiber, etc. When the light shines on the optical coupling device, it undergoes attenuation and total internal reflection to generate surface plasmons for detection.

[0036] The SPR sensor 16 is a Kretschmann prism with a gold coating. The body 1 has a groove 15 for placing the SPR sensor 16, and the mounting block 12 on the side of the cover plate 11 is intermittently inserted into the groove 15.

[0037] The SPR sensor 16 is the site that converts concentration signals into optical signals. It uses a prism coated with a gold film as the SPR sensor 16 and adopts a Kretschmann-type device. Through the cylindrical integrated sensor design, it selects various types of biometric units to modify multifunctional nanomaterials, thereby improving the number of times the sensor can be used and its sensitivity.

[0038] The optical transmission system is located within the mounting block 12. The optical transmission system includes a collimator and a polarizer located in front of the optical coupling system, a convex lens located behind the optical coupling system, and a CCD miniature spectrometer detector in the optical detection system.

[0039] By setting up an optical transmission system and a detection system, the device can be adjusted to a suitable light source to generate plasma for sample detection.

[0040] Example 2

[0041] Reference Figures 2-7 Based on Embodiment 1, the sample introduction system further includes a mounting box 2, which is fixedly connected to the side wall of the machine body 1. A liquid delivery pipe 101 and a liquid discharge pipe 102 are fixedly connected inside the machine body 1. Both the liquid delivery pipe 101 and the liquid discharge pipe 102 are connected to the groove 15. A turntable 22 is rotatably connected inside the mounting box 2. A clamping plate 23 for clamping the liquid delivery pipe 101 is fixedly connected to the side wall of the turntable 22. An arc-shaped block 21 that fits against the liquid delivery pipe 101 is fixedly connected inside the mounting box 2. Three rollers 24 that alternately abut against the liquid delivery pipe 101 are rotatably connected to the side wall of the turntable 22.

[0042] One end of the liquid delivery tube 101 is connected to the sample to be tested, and the other end extends into the groove 15. When the cover plate 11 is closed, a sealed space is formed in the groove 15. The sample solution pumped into the groove 15 can squeeze out the air in the groove 15 through the drain tube 102, thereby removing the air in the groove 15 and improving the reproducibility of the experimental structure. When the turntable 22 is rotated, the arc block 21 supports the liquid delivery tube 101. The roller 24 on the turntable 22 alternately squeezes the tube wall of the liquid delivery tube 101, so that the liquid delivery tube 101 draws up the sample solution and delivers it into the groove 15. The sample solution flows over the surface of the SPR sensor 16 in the groove 15. The retaining plate 23 on the side wall of the turntable 22 can prevent the liquid delivery tube 101 from detaching from the turntable 22, thereby ensuring the stable squeezing of the liquid delivery tube 101 by the roller 24.

[0043] A rotating rod 25 is coaxially fixedly connected to the turntable 22. A worm gear 26 is fixedly connected to the end of the rotating rod 25. A connecting seat 18 is fixedly connected to the side wall of the machine body 1. A worm 27 that meshes with the worm gear 26 is rotatably connected to the connecting seat 18. A first handle 28 is fixedly connected to the bottom of the worm 27.

[0044] By manually controlling the first handle 28, the worm 27 can be rotated. At this time, the worm 27 drives the worm wheel 26, which meshes with it, to rotate. Since the worm wheel 26 is fixedly connected to the turntable 22 through the rotating rod 25, the turntable 22 is driven to rotate by the worm wheel 26. The rotation of the turntable 22 is transmitted through the meshing of the worm wheel 26 and the worm 27, which improves the rotational accuracy of the turntable 22, thereby ensuring the accuracy of pumping the sample solution and making the experimental results more accurate.

[0045] Two symmetrically arranged support rods 31 are fixedly connected to the side of the body 1. A base 3 is slidably connected to the two support rods 31. A first motor 34 is fixedly connected to the middle of the base 3. A drive shaft 35 is fixedly connected to the output end of the first motor 34. A hexagonal prism 36 is fixedly connected to the end of the drive shaft 35. The hexagonal prism 36 is intermittently inserted into the top of the worm gear 27. A screw 32 is rotatably connected to the upper surface of the body 1. The screw 32 is threaded to the side of the base 3. A second handle 33 is fixedly connected to the top of the screw 32.

[0046] The support rod 31 allows the base 3 to be installed. Rotating the second handle 33 causes the screw 32 to rotate, which is used for the threaded connection between the screw 32 and the base 3. The support rod 31 also limits the base 3, preventing it from rotating on its own. When the screw 32 rotates, it can drive the base 3 to rise and fall. At this time, the first motor 34 on the base 3 rises and falls synchronously, allowing the drive shaft 35 at the output end of the first motor 34 to move closer to or away from the worm gear 27. This allows the hexagonal prism 36 to be inserted into the worm gear 27. When the hexagonal prism 36 is inserted into the worm gear 27, starting the first motor 34 will cause the worm gear 27 to rotate, thereby increasing the rotational speed of the worm gear 27. This ensures that the flow rate of the sample solution meets the requirements when a large flow rate of sample solution is needed for flow detection.

[0047] Example 3

[0048] Reference Figure 1 , Figure 2 , Figure 8 , Figure 9 and Figure 10 Based on Embodiment 1, a further feature is that a first mounting plate 46 is rotatably connected to the side of the machine body 1 away from the mounting box 2, a nut 48 is threadedly connected to the first mounting plate 46, a threaded hole is provided on the machine body 1 to mate with the nut 48, a receiving cylinder 45 is fixedly connected to one side of the first mounting plate 46, a rotating column 47 is fixedly connected to the other side of the first mounting plate 46, a rotating hole is provided on the machine body 1 to mate with the rotating column 47, one end of the drain pipe 102 away from the groove 15 extends into the receiving cylinder 45, a discharge pipe 451 is fixedly connected to the bottom of the receiving cylinder 45, and a valve 452 is provided on the discharge pipe 451.

[0049] Since the end of the drain pipe 102 extends into the receiving cylinder 45, the sample solution after testing can be discharged into the receiving cylinder 45 and received. By opening the valve 452 on the drain pipe 451 at the bottom of the receiving cylinder 45, the sample solution can be discharged. The first mounting plate 46 is connected to the machine body 1 through the rotating column 47. When the nut 48 is removed from the first mounting plate 46, the first mounting plate 46 can rotate relative to the machine body 1. At this time, the receiving cylinder 45, which is fixedly connected to the side of the first mounting plate 46, can rotate, so that the sample after testing received in the receiving cylinder 45 can be quickly poured out.

[0050] A bracket 5 is rotatably connected to the body 1 on the opposite side of the cover plate 11. A support block 17 for supporting the bracket 5 is fixedly connected to the upper surface of the body 1. A second mounting plate 51 is fixedly connected to the end of the bracket 5. Multiple brush rollers 52 are rotatably connected to the second mounting plate 51. Each brush roller 52 has a pulley 53 fixedly connected to its top. A belt 54 drives the pulleys 53 together. A limit post 55 is rotatably connected to the upper surface of the second mounting plate 51. The limit post 55 is in contact with the belt 54. A mounting frame 58 is fixedly connected to the upper part of the bracket 5. A second motor 56 is fixedly connected to the middle part of the mounting frame 58. An output shaft 57 is fixedly connected to the output end of the second motor 56. One of the pulleys 53 is fixedly connected to the bottom of the output shaft 57.

[0051] After the test is completed, open the cover plate 11 and remove the SPR sensor 16 from the groove 15. Then rotate the bracket 5 so that the second mounting plate 51 at the end of the bracket 5 moves to the upper part of the groove 15. At this time, the brush roller 52 on the second mounting plate 51 can be inserted into the groove 15. The support block 17 can support the bracket 5 and prevent the brush roller 52 on the support block 17 at the end of the bracket 5 from making hard contact with the inner wall of the groove 15, which would make it difficult for the brush roller 52 to rotate. The second motor 56 is a dual-axis motor. Starting the second motor 56 causes the output shaft 57 to rotate. The output shaft 57 is fixedly connected to a pulley 53, which rotates. Since multiple pulleys 53 are connected by a belt 54, when one pulley 53 rotates, it can drive the other pulleys 53 to rotate synchronously through the belt 54, thus causing all the brush rollers 52 to rotate. At this time, clean water is sent into the end of the liquid delivery pipe 101, and the rotation of the turntable 22 can be controlled so that the clean water enters the groove 15 to clean the groove 15. The limiting post 55 squeezes the belt 54, making the connection between the belt 54 and the pulley 53 more secure.

[0052] A first piston cylinder 65 is fixedly connected to the upper part of the mounting bracket 58. A piston plate 63 is slidably connected inside the first piston cylinder 65. A protrusion 64 is provided on the side wall of the piston plate 63. A limiting groove that mates with the protrusion 64 is opened on the inner wall of the first piston cylinder 65. A cylinder 61 is fixedly connected to the lower surface of the piston plate 63. A cylindrical cam 6 is fixedly connected to the end of the output shaft 57 away from the pulley 53. A slider 62 is fixedly connected inside the cylinder 61. The slider 62 is slidably connected in a groove on the side wall of the cylindrical cam 6. An L-shaped frame 4 is fixedly connected to the side of the machine body 1 near the receiving cylinder 45. Two symmetrically arranged slide rods 41 are slidably connected to the L-shaped frame 4. A circular plate 42 is fixedly connected to the bottom. The drain pipe 102 is fixedly connected to the circular plate 42. The side wall of the circular plate 42 is intermittently attached to the side wall of the receiving cylinder 45. A piston rod 43 is fixedly connected to the middle of the circular plate 42. A second piston cylinder 44 is fixedly connected to the upper surface of the L-shaped frame 4. The piston rod 43 is slidably connected inside the second piston cylinder 44. A first pipe 66 is fixedly connected between the first piston cylinder 65 and the second piston cylinder 44. A second pipe 67 and a third pipe 441 are fixedly connected to the first piston cylinder 65 and the second piston cylinder 44, respectively. A one-way valve 68 is provided on both the first pipe 66 and the second pipe 67. A vent valve 442 is provided on the third pipe 441.

[0053] When the second motor 56 runs, the cylindrical cam 6 rotates synchronously. Since the first piston cylinder 65 is fixedly connected to the bracket 5 via the mounting bracket 58, the first piston cylinder 65 will not rotate. The protrusion 64 on the side wall of the piston plate 63 limits the piston plate 63, allowing the piston plate 63 to slide vertically within the first piston cylinder 65 without rotating relative to it. Therefore, when the cylindrical cam 6 rotates, the slider 62 on the inner wall of the cylinder 61 can slide within the groove on the outer wall of the cylindrical cam 6, causing the piston plate 63 to slide back and forth within the first piston cylinder 65. This allows external air to be drawn through the first pipe 66, and the drawn air is discharged through the second pipe 67. During the cleaning of the groove 15, the second... The vent valve 442 on the third pipe 441 on the piston cylinder 44 and the valve 452 on the discharge pipe 451 push the circular plate 42, causing the circular plate 42 to enter the bottom of the receiving cylinder 45. Then, the valve 452 is closed, and the vent valve 442 is closed. At this time, the first piston cylinder 65 intermittently extracts the air in the second piston cylinder 44 through the first pipe 66, causing the piston rod 43 in the second piston cylinder 44 to slide upward slowly. At this time, the circular plate 42 slides upward, and since the drain pipe 102 is fixedly connected to the circular plate 42, a negative pressure is generated in the receiving cylinder 45. At this time, the receiving cylinder 45 extracts the cleaned solution from the groove 15, keeping the groove 15 clean so that it will not be interfered with in the next test.

[0054] Working principle: This is a veterinary drug residue detection device based on SPR technology. When in use, the main body 1 is connected to a computer. The sample to be tested (such as milk) is pumped into the main body 1 through the sample introduction system. The light source system irradiates the sample flowing on the SPR sensor 16 after rotating through the optical transmission system. The reflected light is received by the detection system. The detection system draws a curve through the computer. By observing the curve, it can be determined whether there are pesticide or veterinary drug residues.

[0055] The light source system is used to emit the light required for detection. The optical coupling device can be a grating, prism, optical fiber, etc. The light is attenuated and totally reflected on the optical coupling device to generate surface plasmons for detection. The SPR sensor 16 is the place where the concentration signal is converted into an optical signal. A prism coated with a gold film is used as the SPR sensor 16. The Kretschmann type device is adopted. Through the cylindrical integrated sensor design, various types of biometric units are selected to modify the multifunctional nanomaterials to improve the number of uses and sensitivity of the sensor. By setting up an optical transmission system and a detection system, the device can be adjusted to a suitable light to generate plasma for sample detection.

[0056] One end of the liquid delivery tube 101 is connected to the sample to be tested, and the other end extends into the groove 15. When the cover plate 11 is closed, a sealed space is formed in the groove 15. The sample solution pumped into the groove 15 can squeeze out the air in the groove 15 through the drain tube 102, thereby removing the air in the groove 15 and improving the reproducibility of the experimental structure. When the turntable 22 is rotated, the arc block 21 supports the liquid delivery tube 101. The roller 24 on the turntable 22 alternately squeezes the tube wall of the liquid delivery tube 101, so that the liquid delivery tube 101 draws up the sample solution and delivers it into the groove 15. The sample solution flows over the surface of the SPR sensor 16 in the groove 15. The retaining plate 23 on the side wall of the turntable 22 can prevent the liquid delivery tube 101 from detaching from the turntable 22, thereby ensuring the stable squeezing of the liquid delivery tube 101 by the roller 24.

[0057] By manually controlling the first handle 28, the worm 27 can be rotated. At this time, the worm 27 drives the worm wheel 26, which meshes with it, to rotate. Since the worm wheel 26 is fixedly connected to the turntable 22 through the rotating rod 25, the turntable 22 is driven to rotate by the worm wheel 26. The rotation of the turntable 22 is transmitted through the meshing of the worm wheel 26 and the worm 27, which improves the rotational accuracy of the turntable 22, thereby ensuring the accuracy of pumping the sample solution and making the experimental results more accurate.

[0058] The support rod 31 allows the base 3 to be installed. Rotating the second handle 33 causes the screw 32 to rotate, which is used for the threaded connection between the screw 32 and the base 3. The support rod 31 also limits the base 3, preventing it from rotating on its own. When the screw 32 rotates, it can drive the base 3 to rise and fall. At this time, the first motor 34 on the base 3 rises and falls synchronously, allowing the drive shaft 35 at the output end of the first motor 34 to move closer to or away from the worm gear 27. This allows the hexagonal prism 36 to be inserted into the worm gear 27. When the hexagonal prism 36 is inserted into the worm gear 27, starting the first motor 34 will cause the worm gear 27 to rotate, thereby increasing the rotational speed of the worm gear 27. This ensures that the flow rate of the sample solution meets the requirements when a large flow rate of sample solution is needed for flow detection.

[0059] Since the end of the drain pipe 102 extends into the receiving cylinder 45, the sample solution after testing can be discharged into the receiving cylinder 45 and received. By opening the valve 452 on the drain pipe 451 at the bottom of the receiving cylinder 45, the sample solution can be discharged. The first mounting plate 46 is connected to the machine body 1 through the rotating column 47. When the nut 48 is removed from the first mounting plate 46, the first mounting plate 46 can rotate relative to the machine body 1. At this time, the receiving cylinder 45, which is fixedly connected to the side of the first mounting plate 46, can rotate, so that the sample after testing received in the receiving cylinder 45 can be quickly poured out.

[0060] After the test is completed, open the cover plate 11 and remove the SPR sensor 16 from the groove 15. Then rotate the bracket 5 so that the second mounting plate 51 at the end of the bracket 5 moves to the upper part of the groove 15. At this time, the brush roller 52 on the second mounting plate 51 can be inserted into the groove 15. The support block 17 can support the bracket 5 and prevent the brush roller 52 on the support block 17 at the end of the bracket 5 from making hard contact with the inner wall of the groove 15, which would make it difficult for the brush roller 52 to rotate. The second motor 56 is a dual-axis motor. Starting the second motor 56 causes the output shaft 57 to rotate. The output shaft 57 is fixedly connected to a pulley 53, which rotates. Since multiple pulleys 53 are connected by a belt 54, when one pulley 53 rotates, it can drive the other pulleys 53 to rotate synchronously through the belt 54, thus causing all the brush rollers 52 to rotate. At this time, clean water is sent into the end of the liquid delivery pipe 101, and the rotation of the turntable 22 can be controlled so that the clean water enters the groove 15 to clean the groove 15. The limiting post 55 squeezes the belt 54, making the connection between the belt 54 and the pulley 53 more secure.

[0061] When the second motor 56 runs, the cylindrical cam 6 rotates synchronously. Since the first piston cylinder 65 is fixedly connected to the bracket 5 via the mounting bracket 58, the first piston cylinder 65 will not rotate. The protrusion 64 on the side wall of the piston plate 63 limits the piston plate 63, allowing the piston plate 63 to slide vertically within the first piston cylinder 65 without rotating relative to it. Therefore, when the cylindrical cam 6 rotates, the slider 62 on the inner wall of the cylinder 61 can slide within the groove on the outer wall of the cylindrical cam 6, causing the piston plate 63 to slide back and forth within the first piston cylinder 65. This allows external air to be drawn through the first pipe 66, and the drawn air is discharged through the second pipe 67. During the cleaning of the groove 15, the second... The vent valve 442 on the third pipe 441 on the piston cylinder 44 and the valve 452 on the discharge pipe 451 push the circular plate 42, causing the circular plate 42 to enter the bottom of the receiving cylinder 45. Then, the valve 452 is closed, and the vent valve 442 is closed. At this time, the first piston cylinder 65 intermittently extracts the air in the second piston cylinder 44 through the first pipe 66, causing the piston rod 43 in the second piston cylinder 44 to slide upward slowly. At this time, the circular plate 42 slides upward, and since the drain pipe 102 is fixedly connected to the circular plate 42, a negative pressure is generated in the receiving cylinder 45. At this time, the receiving cylinder 45 extracts the cleaned solution from the groove 15, keeping the groove 15 clean so that it will not be interfered with in the next test.

[0062] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A veterinary drug residue detection device based on SPR technology, comprising a body (1), characterized in that: The body (1) is equipped with a light source system, an SPR sensor (16), a sample introduction system, an optical transmission system and an optical detection system; The body (1) has a groove (15) for placing the SPR sensor (16). The sample introduction system includes a mounting box (2), which is fixedly connected to the side wall of the machine body (1). A liquid delivery pipe (101) and a liquid discharge pipe (102) are fixedly connected inside the machine body (1). The end of the drain pipe (102) away from the groove (15) extends into the receiving cylinder (45), and the bottom of the receiving cylinder (45) is fixedly connected to the discharge pipe (451), and the discharge pipe (451) is provided with a valve (452). A rotating base (14) is fixedly connected to the upper part of the fuselage (1). A rotating shaft (13) is rotatably connected inside the rotating base (14). A cover plate (11) is fixedly connected to the side wall of the rotating shaft (13). A mounting block (12) is fixedly connected to the side of the cover plate (11). The light source system is set inside the mounting block (12). The light source system includes a light coupling device and a light source that illuminates the light coupling device. A bracket (5) is rotatably connected to the body (1) on the opposite side of the cover plate (11). A support block (17) for supporting the bracket (5) is fixedly connected to the upper surface of the body (1). A second mounting plate (51) is fixedly connected to the end of the bracket (5). Multiple brush rollers (52) are rotatably connected to the second mounting plate (51). A pulley (53) is fixedly connected to the top of each of the multiple brush rollers (52). A belt (54) is connected between the multiple pulleys (53). A limit post (55) is rotatably connected to the upper surface of the second mounting plate (51). The limit post (55) is in contact with the belt (54). A mounting frame (58) is fixedly connected to the upper part of the bracket (5). A second motor (56) is fixedly connected to the middle part of the mounting frame (58). An output shaft (57) is fixedly connected to the output end of the second motor (56). One of the pulleys (53) is fixedly connected to the bottom of the output shaft (57). The upper part of the mounting bracket (58) is fixedly connected to a first piston cylinder (65), and a piston plate (63) is slidably connected inside the first piston cylinder (65). A protrusion (64) is provided on the side wall of the piston plate (63). A limiting groove that cooperates with the protrusion (64) is opened on the inner wall of the first piston cylinder (65). A cylinder (61) is fixedly connected to the lower surface of the piston plate (63). A cylindrical cam (6) is fixedly connected to the end of the output shaft (57) away from the pulley (53). A slider (62) is fixedly connected inside the cylinder (61). The slider (62) is slidably connected in the groove on the side wall of the cylindrical cam (6). An L-shaped frame (4) is fixedly connected to the side of the machine body (1) near the receiving cylinder (45). Two symmetrically arranged slide rods (41) are slidably connected to the L-shaped frame (4). A circular plate (42) is fixedly connected to the bottom of the L-shaped frame (4). The drain pipe (102) is fixedly connected to the circular plate (42). The side wall of the circular plate (42) is intermittently attached to the side wall of the receiving cylinder (45). A piston rod (43) is fixedly connected to the middle of the circular plate (42). A second piston cylinder (44) is fixedly connected to the upper surface of the L-shaped frame (4). The piston rod (43) is slidably connected inside the second piston cylinder (44). A first pipe (66) is fixedly connected between the first piston cylinder (65) and the second piston cylinder (44). A second pipe (67) and a third pipe (441) are fixedly connected to the first piston cylinder (65) and the second piston cylinder (44), respectively. A one-way valve (68) is provided on the first pipe (66) and the second pipe (67). A vent valve (442) is provided on the third pipe (441).

2. The veterinary drug residue detection device based on SPR technology according to claim 1, characterized in that: The SPR sensor (16) is a Kretschmann prism, and the Kretschmann prism is coated with a gold film. The mounting block (12) on the side of the cover plate (11) is intermittently embedded in the groove (15).

3. The veterinary drug residue detection device based on SPR technology according to claim 1, characterized in that: The optical transmission system is set inside the mounting block (12). The optical transmission system includes a collimator and a polarizer set in front of the optical coupling system, and a convex lens set behind the optical coupling system. The optical detection system is a CCD miniature spectrometer detector.

4. The veterinary drug residue detection device based on SPR technology according to claim 2, characterized in that: The liquid delivery pipe (101) and the liquid discharge pipe (102) are both connected to the groove (15). A turntable (22) is rotatably connected inside the mounting box (2). A clamping plate (23) for clamping the liquid delivery pipe (101) is fixedly connected to the side wall of the turntable (22). An arc-shaped block (21) that fits against the liquid delivery pipe (101) is fixedly connected inside the mounting box (2). Three rollers (24) that alternately abut against the liquid delivery pipe (101) are rotatably connected to the side wall of the turntable (22).

5. The veterinary drug residue detection device based on SPR technology according to claim 4, characterized in that: A rotating rod (25) is coaxially fixedly connected to the turntable (22). A worm gear (26) is fixedly connected to the end of the rotating rod (25). A connecting seat (18) is fixedly connected to the side wall of the machine body (1). A worm (27) that meshes with the worm gear (26) is rotatably connected to the connecting seat (18). A first handle (28) is fixedly connected to the bottom of the worm (27).

6. The veterinary drug residue detection device based on SPR technology according to claim 5, characterized in that: Two symmetrically arranged support rods (31) are fixedly connected to the side of the body (1). A base (3) is slidably connected to the two support rods (31). A first motor (34) is fixedly connected to the middle of the base (3). A drive shaft (35) is fixedly connected to the output end of the first motor (34). A hexagonal prism (36) is fixedly connected to the end of the drive shaft (35). The hexagonal prism (36) is intermittently inserted into the top of the worm gear (27). A screw (32) is rotatably connected to the upper surface of the body (1). The screw (32) is threaded to the side of the base (3). A second handle (33) is fixedly connected to the top of the screw (32).

7. A veterinary drug residue detection device based on SPR technology according to claim 6, characterized in that: A first mounting plate (46) is rotatably connected to the side of the machine body (1) away from the mounting box (2). A nut (48) is threaded onto the first mounting plate (46). A threaded hole that mates with the nut (48) is provided on the machine body (1). A receiving cylinder (45) is fixedly connected to one side of the first mounting plate (46). A rotating column (47) is fixedly connected to the other side of the first mounting plate (46). A rotating hole that mates with the rotating column (47) is provided on the machine body (1).

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