Micro-magnetic field-based immune tumor cell screening system

Abstract

The application discloses the technical field of immunological tumor cell screening and relates to a micro-magnetic-field-based immunological tumor cell screening system, which comprises a hollow screening body, the screening body is divided into a first screening body and a second screening body with a diagonal line as a center, a magnet is fixedly connected to the side wall of the first screening body, a discharge port is arranged at the contact position of the bottom of the first screening body and the second screening body, a feeding port is arranged at the top of the second screening body, a plurality of first screening plates are vertically arranged along the inner side wall of the first screening body, the lengths of the first screening plates gradually increase from top to bottom in a stepped manner, a plurality of second screening plates are vertically arranged along the inner side wall of the second screening body, the lengths of the second screening plates gradually decrease from top to bottom in an inverted stepped manner, gaps are arranged between the first screening plates and the second screening plates, and the feeding port and the discharge port are in communication with the gaps. The application has the advantages of simple structure and the design of the trapezoidal screening plates, which can delay the residence time of tumor cells.

Description

Technical Field

[0001] This invention belongs to the field of immunotumor cell screening technology, specifically an immunotumor cell screening system based on a micromagnetic field. Background Technology

[0002] Blood biochips are a next-generation diagnostic tool for detecting rare cells in the blood associated with serious diseases. These rare cells include circulating tumor cells (CTCs), malignant stem cells, and diseased cells with specific protein markers. Related studies have confirmed a strong correlation between the number of circulating tumor cells in a patient's blood sample and early diagnosis and survival rates. Therefore, the detection and analysis of rare cells in a patient's blood sample is crucial for improving the early detection rate of cancer and personalized treatment.

[0003] Magnetic nanoparticles with antibodies on their surface are placed in a blood sample and adsorbed onto target cells through an immune response. The blood sample, in which rare cells have been labeled, is then passed through a microfluidic channel. The rare cells in the blood sample, which are attached to the magnetic nanoparticles, are guided by a gradient magnetic field emitted by a magnetic body placed below the biochip and collected onto a glass slide. Since the slide and the thin film are detachably connected, the user can easily separate the slide and the thin film after the collection is completed, remove the slide, and perform subsequent analysis on the rare cells on the slide.

[0004] A magnetic material is placed outside the microfluidic channel to separate tumor cells labeled with magnetic nanoparticles. This biochip structure has the following drawbacks during use: 1. Free nanoparticles and labeled tumor cells tend to be adsorbed and aggregated within a small area on the slide. This aggregation may interfere with or even damage the tumor cells during recognition. 2. The labeled target tumor cells roll within the weak magnetic field area and escape from the microfluidic channel without being captured by the magnetic material.

[0005] To address the aforementioned issues, Chinese Patent Publication No. CN203144415U discloses an immunotumor cell screening system based on a micromagnetic field, comprising a microchip, a permanent magnet, and a microparamagnetic array. The microchip includes a thin sheet and a glass slide, forming a closed microfluidic channel between the thin sheet and the glass slide. The microparamagnetic array is located on the glass slide within the microfluidic channel, while the permanent magnet is positioned outside the microfluidic channel near the outer surface of the glass slide. The magnetic field of the permanent magnet passes through the glass slide and acts on the microparamagnetic array. Its advantages include the ability of the microparamagnetic array to form a more uniform magnetic field within the microfluidic channel, resulting in a more uniform distribution of labeled nanoparticle-laden tumor cells on the glass slide, preventing damage to tumor cells due to excessive aggregation, and making the subsequent identification process more convenient and efficient.

[0006] The aforementioned device utilizes microchips, permanent magnets, and microparamagnetic arrays to achieve uniform adsorption of tumor cells. However, during actual operation, the blood sample is affected by gravity and flows out of the outlet relatively quickly. This results in some tumor cells flowing out with gravity before they can be labeled on the slide. Summary of the Invention

[0007] To address the aforementioned problems, the present invention aims to provide an immune tumor cell screening system based on a micromagnetic field, which delays the residence time of tumor cells through the design of a trapezoidal screening plate.

[0008] To achieve the above objectives, the technical solution of the present invention is as follows: an immune tumor cell screening system based on a micromagnetic field, comprising a hollow screening body, the screening body being divided into a first screening body and a second screening body with the diagonal as the center, both the first screening body and the second screening body being in the shape of a right triangle; A magnet is fixedly connected to the side wall of the first screening body. A discharge port is provided at the bottom of the first screening body where it contacts the second screening body. A feed port is provided at the top of the second screening body, and the feed port is located diagonally opposite the discharge port. The first screening body is composed of several first screening plates arranged vertically along its inner side wall. The length of the several first screening plates increases sequentially from top to bottom in a stepped shape. The second screening body is composed of several second screening plates arranged vertically along its inner side wall. The length of the several second screening plates decreases sequentially from top to bottom in an inverted stepped shape. There is a gap between the several first screening plates and the several second screening plates. The feed port and the discharge port are both connected to the gap. Several guide rods are provided on the uncovered parts of the first screening plates and the second screening plates.

[0009] The beneficial effects of the basic scheme are: 1. Because the first and second screening plates are trapezoidal and inverted trapezoidal, when the blood sample flows in the gap, it can slow down the flow speed of tumor cells to a certain extent, so that the tumor cells have enough time to be adsorbed on the first and second screening plates.

[0010] 2. Because the length of the first screening plates increases sequentially from top to bottom, the magnetic force of the longer first screening plates located at the bottom of the first screening body is relatively small. This can match the characteristic that the more the blood sample flows to the lower part of the gap, the fewer tumor cells are in the blood sample. Thus, the magnetic force can be set in proportion to the amount of tumor cells, thereby enabling tumor cells to be screened efficiently.

[0011] 3. Because both the first and second screening plates are equipped with several guide rods, the guide rods can slow down the flow of blood samples when they flow onto the first and second screening plates. This allows tumor cells to be fully adsorbed onto the first and second screening plates, thereby improving the efficiency of tumor cell screening.

[0012] Furthermore, the first and second screening bodies are detachably connected by bolts.

[0013] The beneficial effect of the basic scheme is that removing the bolts allows the first and second screening bodies to be disassembled, thereby allowing the tumor cells screened on the first and second screening plates to be directly removed.

[0014] Furthermore, a number of first screening plates are detachably connected to the first screening body by bolts, and a number of second screening plates are detachably connected to the second screening body by bolts. Several adjacent first screening plates are detachably connected by adhesive, and several adjacent second screening plates are detachably connected by adhesive.

[0015] The beneficial effect of the basic scheme is that the number of the first screening plate and the second screening plate can be adjusted, thereby allowing the device to adjust the number of screening plates according to different screening quantities, thus improving the screening efficiency.

[0016] Furthermore, several guide rod arrays are distributed on the first screening plate and the second screening plate, with the same spacing between adjacent vertical guide rods and the increasing spacing between adjacent horizontal guide rods from left to right.

[0017] The beneficial effect of the basic scheme is that tumor cells of different sizes in the blood sample will flow through the gaps between different guide rods, and thus tumor cells of different sizes will be specifically screened to different guide rod gaps. This allows for screening of tumor cells by both size and size.

[0018] Furthermore, it also includes a push plate, which is fixedly connected to the side of the second screening plate away from the first screening plate. The push plate passes through the side wall of the second screening body and extends out of the second screening body, and the push plate slides in cooperation with the second screening body. The top of the second screening body is provided with a sliding groove, and the top of the second screening plate near the top of the second screening body is provided with a slider corresponding to the sliding groove. Several second screening plates slide in cooperation with the second screening body through the sliding groove and the slider.

[0019] The beneficial effects of the basic scheme are: 1. Squeezing tumor cells by hand can improve the efficiency of tumor cell screening and enhance the accuracy of the size of the screened tumor cells.

[0020] 2. The size of the gap between the first and second screening plates can be adjusted, thereby adjusting the flow speed of the blood sample in the gap according to the actual screening needs, so that tumor cells can be screened efficiently.

[0021] Furthermore, a limit plate is fixedly connected to the side wall of the push plate.

[0022] The beneficial effect of the basic scheme is that the design of the limiting plate can limit the maximum stroke of the push plate, thereby reducing the possibility of tumor cell damage.

[0023] Furthermore, a pull block is fixedly connected to the side of the push plate away from the second screening plate, and the pull block is provided with anti-slip texture.

[0024] The advantages of the basic design are: it makes it easy to pull and push the push plate for operation, and the anti-slip texture design reduces the possibility of slipping.

[0025] Furthermore, a telescopic rod is fixedly connected to the top of the slider. The telescopic rod passes through the slide groove and extends to the body of the second screening body. A pressing block is rotatably connected to the end of the telescopic rod away from the slider. Several locking blocks are connected to the bottom of the pressing block. Several locking slots corresponding to the locking blocks are provided on the top of the second screening body. An elastic layer is provided in the locking slots, and anti-slip textures are provided on the locking blocks.

[0026] The beneficial effect of the basic scheme is that it can fix several second screening plates, thereby reducing the possibility of the second screening plates shifting under the flow of blood. Attached Figure Description

[0027] Figure 1 This is a frontal cross-sectional view of the micromagnetic field-based immune tumor cell screening system in an embodiment of the present invention.

[0028] Figure 2 for Figure 1 The top view in the image.

[0029] Figure 3 for Figure 1 The top view of the second filter panel.

[0030] Figure 4 for Figure 1 Axonometric view of the pull block in the diagram.

[0031] The reference numerals in the accompanying drawings include: second screening body 1, slide 2, slider 3, telescopic rod 4, pressing block 5, locking block 6, feed port 7, first screening body 8, magnet 9, first screening plate 10, gap 11, discharge port 12, push plate 13, limiting plate 14, second screening plate 15, pull block 16, guide rod 17, and locking groove 19. Detailed Implementation

[0032] The following detailed description illustrates the specific implementation method: Example 1 The basics are as follows: Figure 1 As shown: An immune tumor cell screening system based on micromagnetic field includes a hollow screening body, which is divided into a first screening body 8 and a second screening body 1 with the diagonal as the center. Both the first screening body 8 and the second screening body 1 are right-angled triangular bodies. A magnet 9 is fixedly connected to the side wall of the first screening body 8. A discharge port 12 is provided at the bottom of the first screening body 8 where it contacts the second screening body 1. A feed port 7 is provided at the top of the second screening body 1. The feed port 7 is located on the diagonal of the discharge port 12. The first screening body 8 is composed of several first screening plates 10 arranged vertically along its inner side wall. The length of the several first screening plates 10 increases from top to bottom in a stepped shape. The second screening body 1 is composed of several second screening plates 15 arranged vertically along its inner side wall. The length of the several second screening plates 15 decreases from top to bottom in an inverted stepped shape. A gap 11 is provided between the several first screening plates 10 and second screening plates 15. The feed port 7 and the discharge port 12 are both connected to the gap 11. Several guide rods 17 are provided on the uncovered parts of the first screening plates 10 and second screening plates 15.

[0033] The specific implementation process is as follows: During operation, the blood sample is first put into the inlet 7, and then the blood sample will flow down through the gap 11. Due to the setting of the magnet 9, when the blood sample flows down the steps, the tumor cells with magnetic nanoparticles in the blood sample are guided by the gradient magnetic field emitted by the magnet 9. As a result, the tumor cells will be retained on several first screening plates 10 and second screening plates 15, while the rest of the unwanted parts will flow out from the outlet 12. Because the first screening plate 10 and the second screening plate 15 are trapezoidal and inverted trapezoidal respectively, when the blood sample flows within the gap 11, the flow rate of tumor cells can be slowed down to a certain extent. This allows sufficient time for tumor cells to be adsorbed onto the first screening plate 10 and the second screening plate 15. Furthermore, since the lengths of the first screening plates 10 increase sequentially from top to bottom, the magnetic properties of the longer first screening plates 10 located at the bottom of the first screening body 8 are relatively weak. This aligns with the characteristic that the lower the blood sample flows into the gap 11, the fewer tumor cells remain in the blood sample. Consequently, the magnetic force is proportional to the amount of tumor cells, thus enabling efficient screening of tumor cells. Simultaneously, because both the first screening plate 10 and the second screening plate 15 are equipped with several guide rods 17, the design of the guide rods 17 can slow down the flow of the blood sample when it flows onto the first screening plate 10 and the second screening plate 15. This allows tumor cells to be fully adsorbed onto the first screening plate 10 and the second screening plate 15, thereby improving the efficiency of tumor cell screening.

[0034] Example 2 The difference from the above embodiments is that the first screening body 8 and the second screening body 1 are detachably connected by bolts.

[0035] The specific implementation process is as follows: After the tumor cells have been screened, the first screening body 8 and the second screening body 1 can be disassembled by removing the bolts, and then the tumor cells screened on the first screening plate 10 and the second screening plate 15 can be directly removed.

[0036] Example 3 The difference from the above embodiments is that a plurality of first screening plates 10 are detachably connected to the first screening body 8 by bolts, and a plurality of second screening plates 15 are detachably connected to the second screening body 1 by bolts. Several adjacent first screening plates 10 are detachably connected by adhesive, and several adjacent second screening plates 15 are detachably connected by adhesive.

[0037] The specific implementation process is as follows: By removing the bolts, the number of the first screening plate 10 and the second screening plate 15 can be adjusted, thereby allowing the device to adjust the number of screening plates according to different screening quantities, thus improving the screening efficiency.

[0038] Example 4 The difference from the above embodiments is that a plurality of guide rods 17 are arrayed on the first screening plate 10 and the second screening plate 15, the spacing between adjacent vertical guide rods 17 is the same, and the spacing between adjacent horizontal guide rods 17 increases from left to right.

[0039] The specific implementation process is as follows: When the blood sample flows to the first screening plate 10 and the second screening plate 15, because the spacing between adjacent transverse guide rods 17 increases from left to right, tumor cells of different sizes in the blood sample will flow through the gaps 11 between different guide rods 17 respectively. Thus, tumor cells of different sizes will be specifically screened to the gaps 11 between different guide rods 17. This allows for the screening of tumor cells by size at the same time.

[0040] Example 5 The difference from the above embodiment is that it also includes a push plate 13, which is fixedly connected to the right side of the second screening plate 15. The push plate 13 passes through the side wall of the second screening body 1 and extends to the outside of the second screening body 1, and the push plate 13 is slidably engaged with the second screening body 1. The top of the second screening body 1 is provided with a groove 2, and the top of the second screening plate 15 near the top of the second screening body 1 is provided with a slider 3 corresponding to the groove 2. Several second screening plates 15 are slidably engaged with the second screening body 1 through the groove 2 and the slider 3.

[0041] The specific implementation process is as follows: When the blood sample flows on the screening plate, the push plate 13 can be pressed by hand, and the second screening body 1 will move laterally towards the first screening plate 10 under the pushing force of the push plate 13. As a result, the gap 11 will be reduced, so the second screening plate 15 will squeeze the tumor cells. After the tumor cells are squeezed, the tumor cells in the center of the first screening plate 10 and the second screening plate 15 will move to both sides. Since the guide rods 17 have different horizontal and vertical spacing, when larger tumor cells flow to the guide rods 17 with larger spacing, they will stop there, thus achieving the screening of tumor cells of different sizes. At the same time, squeezing the tumor cells by hand can improve the efficiency of tumor cell screening and enhance the accuracy of the size of the screened tumor cells. Furthermore, by sliding the position of the second screening plate 15, the size of the gap 11 between the first screening plate 10 and the second screening plate 15 can be adjusted, thereby adjusting the flow speed of the blood sample in the gap 11 according to the actual screening needs, so that tumor cells can be efficiently screened.

[0042] Example 6 The difference from the above embodiment is that the push plate 13 is fixedly connected to the side wall of the limit plate 14.

[0043] The specific implementation process is as follows: In order to reduce the possibility of damage to the therapeutic cells due to excessive distance of the pushed plate 13, it is necessary to limit the pushing distance of the pushed plate 13. Therefore, the design of the limiting plate 14 can limit the maximum stroke of the pushed plate 13, thereby reducing the possibility of tumor cell damage.

[0044] Example 7 The difference from the above embodiment is that a pull block 16 is fixedly connected to the right side of the push plate 13, and the pull block 16 is provided with anti-slip texture.

[0045] The specific implementation process is as follows: The design of the pull block 16 makes it easy to pull and push the push plate 13 for operation, and the anti-slip texture design reduces the possibility of slipping.

[0046] Example 8 The difference from the above embodiment is that a telescopic rod 4 is fixedly connected to the top of the slider 3. The telescopic rod 4 passes through the slide groove 2 and extends to the outside of the second screening body 1. A pressing block 5 is rotatably connected to the end of the telescopic rod 4 away from the slider 3. Several locking blocks 6 are connected to the bottom of the pressing block 5. Several locking grooves 19 corresponding to the locking blocks 6 are provided on the top of the second screening body 1. An elastic layer is provided in the locking grooves 19. Anti-slip texture is provided on the locking blocks 6.

[0047] The specific implementation process is as follows: After the second screening plate 15 is pushed to the appropriate position, the pressing block 5 is rotated by hand, and then the locking block 6 on the pressing block 5 is aligned with the slot 19. Then, the pressing block 5 is pressed, and the locking block 6 will be locked in the slot 19. This can fix several second screening plates 15, thereby reducing the possibility of the second screening plates 15 shifting under blood flow. At the same time, when it is necessary to push the push plate 13 and adjust the position of the second screening plate 15, the pressing block 5 can be pulled up by hand, which will disengage the locking block 6 from the slot 19, thereby allowing the second screening plate 15 to move flexibly laterally.

[0048] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0049] The above descriptions are merely embodiments of the present invention. Commonly known structures and characteristics are not described in detail here. Those skilled in the art are aware of all common technical knowledge in the field prior to the application date or priority date, are aware of all existing technologies in that field, and have the ability to apply conventional experimental methods prior to that date. Those skilled in the art can, under the guidance of this application, improve and implement this solution in combination with their own capabilities. Some typical known structures or methods should not be obstacles for those skilled in the art to implement this application. It should be noted that those skilled in the art can make several modifications and improvements without departing from the structure of the present invention. These should also be considered within the scope of protection of the present invention, and will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A micromagnetic field-based immune tumor cell screening system, characterized in that: It includes a hollow screening body, which is divided into a first screening body and a second screening body with the diagonal as the center. Both the first screening body and the second screening body are right-angled triangular bodies. A magnet is fixedly connected to the side wall of the first screening body. A discharge port is provided at the bottom of the first screening body where it contacts the second screening body. A feed port is provided at the top of the second screening body. The feed port is located on the diagonal of the discharge port. A number of first screening plates are vertically arranged along the inner side wall of the first screening body. The length of the number of first screening plates increases from top to bottom in a stepped shape. A number of second screening plates are vertically arranged along the inner side wall of the second screening body. The length of the number of second screening plates decreases from top to bottom in an inverted stepped shape. There is a gap between the number of first screening plates and second screening plates. The feed port and the discharge port are both connected to the gap. A number of guide rods are provided on the uncovered parts of the first screening plates and second screening plates. Several guide rod arrays are distributed on the first screening plate and the second screening plate. The spacing between adjacent vertical guide rods is the same, and the spacing between adjacent horizontal guide rods increases from left to right. It also includes a push plate, which is fixedly connected to the side of the second screening plate away from the first screening plate. The push plate passes through the side wall of the second screening body and extends out of the second screening body, and the push plate slides in cooperation with the second screening body. The top of the second screening body is provided with a sliding groove, and the top of the second screening plate near the top of the second screening body is provided with a slider corresponding to the sliding groove. Several second screening plates slide in cooperation with the second screening body through the sliding groove and the slider.

2. The micromagnetic field-based immunotumor cell screening system according to claim 1, characterized in that: The first and second screening bodies are detachably connected by bolts.

3. The micromagnetic field-based immunotumor cell screening system according to claim 2, characterized in that: A number of first screening plates are detachably connected to the first screening body by bolts, and a number of second screening plates are detachably connected to the second screening body by bolts; Several adjacent first screening plates are detachably connected by adhesive, and several adjacent second screening plates are detachably connected by adhesive.

4. The micromagnetic field-based immunotumor cell screening system according to claim 3, characterized in that: A limit plate is fixedly connected to the side wall of the push plate.

5. The micromagnetic field-based immunotumor cell screening system according to claim 4, characterized in that: A pull block is fixedly connected to the side of the push plate away from the second screening plate, and the pull block is provided with anti-slip texture.

6. The micromagnetic field-based immunotumor cell screening system according to claim 5, characterized in that: A telescopic rod is fixedly connected to the top of the slider. The telescopic rod passes through the slide groove and extends to the body of the second screening body. A pressing block is rotatably connected to the end of the telescopic rod away from the slider. Several locking blocks are connected to the bottom of the pressing block. Several locking slots corresponding to the locking blocks are provided on the top of the second screening body. An elastic layer is provided in the locking slots. Anti-slip textures are provided on the locking blocks.

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