单胺氧化酶的突变体及其在制备巴洛西韦中的应用

By performing partial site mutations on monoamine oxidase, a monoamine oxidase mutant that efficiently catalyzes the intermediate of baloxivide was formed, solving the problems of low enzyme activity and poor stability in the existing technology, and realizing efficient catalysis and low-cost baloxivide production.

CN116496999BActive Publication Date: 2026-07-17ZHEJIANG ZANYUAN BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG ZANYUAN BIOTECHNOLOGY CO LTD
Filing Date
2022-01-18
Publication Date
2026-07-17

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Abstract

本申请公开了一种单胺氧化酶的突变体及其在制备巴洛西韦中的应用，所述突变体是具有如SEQ ID NO：1所示氨基酸序列的单胺氧化酶发生部分位点突变而形成的，所述部分位点为：E145、I246、Y188、E348或L314中的至少一种，所述突变体具有单胺氧化酶活性，所述突变体能够高效催化底物6,6‑二甲基‑3‑氮杂二环[3.1.0]己烷、氧化剂以及亚硫酸氢盐接触发生不对称氧化‑加成反应，生成巴洛西韦中间体(1S,5S)6,6‑二甲基‑3‑氮杂二环[3.1.0]己烷‑2‑磺酸盐，底物转化率可达99.99％，将所述突变体应用于巴洛西韦的制备工艺中有利于降低生产成本，提高产物得率。
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Citation Information

Patent Citations

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