A plant extract composition as a pesticide and a method of preparing and use thereof

Abstract

This invention provides a plant extract composition as an insecticide, its preparation method, and its uses, belonging to the field of pest control. The composition consists of the following components in the indicated weight ratios: 1-5 parts of Aconitum carmichaelii petroleum ether extract and 1-5 parts of Ginkgo biloba petroleum ether extract. This composition exhibits excellent insecticidal activity against lepidopteran insects, especially against common pests of field crops, achieving a kill rate of over 90%. Compared to using Aconitum carmichaelii petroleum ether extract or Ginkgo biloba petroleum ether extract alone, it demonstrates a synergistic insecticidal effect. Furthermore, this insecticide composition has no acute toxicity. As an agricultural insecticide, this composition is safe to use and has a rapid and potent insecticidal effect against pests. In addition, the preparation method of this composition is simple, easy to operate, and highly efficient, suitable for industrial production. It can be developed as a novel plant-derived pesticide with rapid action, excellent insecticidal effect, high economic benefits, and diverse active ingredients.

Description

Technical Field

[0001] This invention belongs to the field of pest control, specifically relating to a plant extract composition as an insecticide, its preparation method, and its uses. Background Technology

[0002] Pesticides play a vital role in agricultural production. However, traditional chemical pesticides kill beneficial organisms, disrupt ecological balance, and easily lead to pesticide resistance, rendering them ineffective in controlling pests. Furthermore, pesticide residues or improper application can cause poisoning and even death, leading to widespread skepticism towards chemical pesticides. With increasing public awareness of health and environmental issues and a growing demand for green food, people have gained a deeper understanding of plant-derived pesticides. Therefore, the development of new, highly effective, low-toxicity, and multi-modal plant-derived pesticides is urgently needed.

[0003] Plant-derived pesticides contain natural substances as their active ingredients, not artificially synthesized chemicals. They are fast-acting, long-lasting, and have a smooth degradation pathway in nature after application, resulting in minimal environmental pollution. Furthermore, the diverse insecticidal components in plant-derived insecticides make it difficult for pests to develop resistance. They generally exhibit high selectivity, low toxicity to humans, livestock, and natural enemies, and relatively low development and usage costs. These characteristics make plant-derived pesticides one of the more ideal alternatives to chemical pesticides.

[0004] Plant-derived pesticides, also known as botanical pesticides, are an important component of biological pesticides. They refer to plant-based preparations that utilize stable active ingredients contained in certain parts of plants (roots, stems, leaves, flowers, or fruits) and, through specific methods, protect recipient plants from or reduce the harm caused by pests, diseases, and weeds. Currently, the insecticidal and fungicidal properties of plants such as rotenone, tripterygium wilfordii, pyrethrum, neem, sophora flavescens, sophora japonica, black nightshade, rhododendron, purslane, and garlic have been discovered and utilized. Among these, rotenone, pyrethrin, and azadirachtin have been studied relatively successfully, and plant-derived pesticides are attracting increasing attention from researchers. Developing more plant-derived pesticides with low toxicity and excellent insecticidal effects is of great significance to agricultural production.

[0005] Aconite (Aconiti lateralis Radix Praeparata) is a processed root of the plant Aconitum carmichaeli, belonging to the Ranunculaceae family. It is pungent, sweet, and extremely hot in nature, holding an important place in traditional Chinese medicine. Aconite is widely used clinically and is hailed as a "superior medicine" and "one of the four pillars of medicine," being a top choice for restoring yang and rescuing from collapse. Furthermore, the diterpenoid alkaloids contained in aconite possess good insecticidal activity and can be developed and applied as a novel biological insecticide. However, aconite is toxic and can easily produce toxic side effects during use, especially when used in large doses as a pesticide, posing significant safety risks.

[0006] Ginkgo is a precious medicinal plant worldwide, with its leaves, fruits, and outer seed coat all possessing medicinal development value, earning it the title of "a living fossil where every part is valuable." Ginkgo extract (EGb) preparations, developed from ginkgo leaves, are currently among the most popular natural medicines. Many nutritional foods are also developed from ginkgo nuts, but the utilization rate of the ginkgo outer seed coat remains relatively low. Chinese patent application CN1398524A discloses the application of ginkgo as a phytotoxic agent, showing that extracts from ginkgo leaves and outer seed coats are effective against various pests, including those of the orders Homoptera and Lepidoptera. However, the efficacy of ginkgo extract as a phytotoxic agent needs further improvement.

[0007] There are currently no reports of using aconite and ginkgo extracts in combination as insecticides. Summary of the Invention

[0008] The purpose of this invention is to provide a plant extract composition as an insecticide, its preparation method, and its use.

[0009] This invention provides a plant extract composition as an insecticide, which consists of the following components in the indicated weight ratios:

[0010] 1-5 parts of aconite petroleum ether extract and 1-5 parts of ginkgo petroleum ether extract.

[0011] Furthermore, the aforementioned plant extract composition comprises the following components in the indicated weight ratios:

[0012] One part of aconite petroleum ether extract and one part of ginkgo petroleum ether extract.

[0013] Furthermore, the preparation method of the aconite petroleum ether extract includes the following steps:

[0014] (1) Crush the aconite root, add an ethanol aqueous solution for ultrasonic extraction, and then soak it.

[0015] (2) Combine the extracts and concentrate them into an extract. Dissolve the extract in water and adjust the pH to 2-3.

[0016] (3) Add petroleum ether for extraction, and concentrate the petroleum ether phase into an extract to obtain the final product.

[0017] Furthermore,

[0018] In step (1), the ratio of Aconitum carmichaelii to ethanol aqueous solution is 1g:10-15mL;

[0019] And / or, in step (1), the volume ratio of ethanol to water in the ethanol-water solution is 9:1;

[0020] And / or, in step (1), the duration of the ultrasound is 1 to 5 hours;

[0021] And / or, in step (1), the extraction time is 48 to 60 hours;

[0022] And / or, in step (1), the extraction is performed 1 to 3 times;

[0023] And / or, in step (2), the pH adjustment is performed using hydrochloric acid;

[0024] And / or, in step (3), the extraction is performed 1 to 5 times.

[0025] Furthermore, the Ginkgo petroleum ether extract is a Ginkgo biloba outer seed coat petroleum ether extract;

[0026] Preferably, the preparation method of the Ginkgo petroleum ether extract includes the following steps:

[0027] 1) Take the outer seed coat of ginkgo fruit, add an ethanol aqueous solution for ultrasonic extraction, and then soak it;

[0028] 2) Combine the extracts, concentrate them, and dissolve them in water;

[0029] 3) Add petroleum ether for extraction, and concentrate the petroleum ether phase to an extract to obtain the final product.

[0030] Furthermore,

[0031] In step 1), the ratio of the outer seed coat of the ginkgo fruit to the ethanol aqueous solution is 1g:10-15mL;

[0032] And / or, in step 1), the volume ratio of ethanol to water in the ethanol-water solution is 9:1;

[0033] And / or, in step 1), the outer seed coat is crushed before the ultrasonic extraction;

[0034] And / or, in step 1), the duration of the ultrasound is 1 to 5 hours;

[0035] And / or, in step 1), the extraction time is 48 to 60 hours;

[0036] And / or, in step 1), the extraction is performed 1 to 3 times;

[0037] And / or, in step 3), the extraction is performed 1 to 5 times.

[0038] The present invention also provides a method for preparing the aforementioned plant extract composition, which includes the following steps:

[0039] Weigh out the petroleum ether extract of Aconitum carmichaelii and the petroleum ether extract of Ginkgo biloba according to the specified weight ratio, mix them, and the product is obtained.

[0040] The present invention also provides the use of the aforementioned plant extract composition in the preparation of insecticides;

[0041] Preferably, the insecticide is an insecticide that kills insects.

[0042] Furthermore, the insect in question is a lepidopteran insect;

[0043] Preferably, the lepidopteran insect is a noctuid moth;

[0044] More preferably, the noctuid moth is the beet armyworm, the cotton bollworm (Spodopteralitura Fabricius), or the armyworm (Mythimna separata).

[0045] The present invention also provides an insecticide, which is a formulation prepared by using the aforementioned plant extract composition as the active ingredient and adding excipients or auxiliary ingredients acceptable in the field of pesticide formulation; or, it is a formulation prepared by using the aforementioned plant extract composition and other pesticides as active ingredients and adding excipients or auxiliary ingredients acceptable in the field of pesticide formulation.

[0046] Preferably, the excipients or auxiliary ingredients acceptable in the field of pesticide formulations are pesticide adjuvants.

[0047] Pesticide adjuvants are auxiliary substances added during the processing or application of pesticide formulations to improve the physicochemical properties of the pesticides; they are also known as pesticide auxiliaries. Pesticide adjuvants themselves generally have no biological activity.

[0048] The pesticide adjuvants include, but are not limited to, the following substances: silica, kaolin, bentonite, talc, diatomaceous earth, dolomite, calcium carbonate, magnesium oxide, chalk, clay, synthetic silicates, magnesia, sepiolite, etc. Besides water, usable liquid diluents include aromatic organic solvents (mixtures of xylene or alkylbenzenes, chlorobenzene, etc.), paraffin (petroleum fractions), alcohols (methanol, propanol, butanol, octanol, glycerol), esters (ethyl acetate, isobutyl acetate, etc.), ketones (cyclohexanone, acetone, acetophenone, isophorone, ethylpentyl ketone, etc.), and amides (N,N-dimethylformamide, N-methylpyrrolidone, etc.). Usable surfactants include alkyl sulfonates, alkyl aryl sulfonates, polyoxyethylene alkylphenols, polyoxyethylene esters of sorbitol, sodium (calcium) lignosulfonate, triethylamine or triethanolamine salts, etc.

[0049] More preferably, the pesticide adjuvant is an excipient;

[0050] And / or, the formulation is a dry powder, paste, solution, emulsifiable concentrate, suspension, water-in-oil emulsion, wettable powder, microemulsion or granules;

[0051] And / or, the other pesticides are organophosphate pesticides, pyrethroid pesticides, carbamate pesticides, or plant-derived pesticides.

[0052] The preparation method of the pesticide formulation of the present invention includes, but is not limited to, the following methods:

[0053] Weigh out the petroleum ether extract of Aconitum carmichaelii, the petroleum ether extract of Ginkgo biloba, and conventional excipients such as sodium dodecylbenzenesulfonate and pyrrolidone, and prepare a microemulsion with an effective ingredient concentration of 1% using conventional processes.

[0054] Weigh out the petroleum ether extract of Aconitum carmichaelii, the petroleum ether extract of Ginkgo biloba, and conventional excipients such as solvent oil, pyrrolidone, and xanthan gum, and prepare an emulsion with an effective ingredient concentration of 1% using conventional processes.

[0055] Weigh out the petroleum ether extract of Aconitum carmichaelii, the petroleum ether extract of Ginkgo biloba, and conventional excipients such as sodium lignosulfonate and kaolin, and prepare water-dispersible granules with an effective ingredient concentration of 2% according to conventional processes.

[0056] Weigh out the petroleum ether extract of Aconitum carmichaelii and the petroleum ether extract of Ginkgo biloba, and mix them with conventional excipients such as calcium dodecylbenzenesulfonate, phenylethylphenol polyoxyethylene ether, and solvent oil to prepare water-dispersible granules with an effective ingredient concentration of 2% using conventional processes.

[0057] Weigh out the petroleum ether extract of Aconitum carmichaelii, the petroleum ether extract of Ginkgo biloba, and conventional excipients such as lignin and diatomaceous earth, and prepare a wettable powder with an effective ingredient concentration of 2% according to conventional processes.

[0058] Weigh out the petroleum ether extract of Aconitum carmichaelii, the petroleum ether extract of Ginkgo biloba, and conventional excipients such as lignin and diatomaceous earth, and prepare a wettable powder with an effective ingredient concentration of 1% according to conventional processes.

[0059] Weigh out the petroleum ether extract of Aconitum carmichaelii, the petroleum ether extract of Ginkgo biloba, and conventional excipients such as lignin and diatomaceous earth, and prepare a wettable powder with an effective ingredient concentration of 5% according to conventional processes.

[0060] Weigh out the petroleum ether extract of Aconitum carmichaelii, the petroleum ether extract of Ginkgo biloba, and azadirachtin, and mix them with conventional excipients such as solvent oil, pyrrolidone, and xanthan gum to prepare an emulsion with an effective ingredient concentration of 12% using conventional processes.

[0061] Weigh out the petroleum ether extract of Aconitum carmichaelii, the petroleum ether extract of Ginkgo biloba, and deltamethrin, and mix them with conventional excipients such as solvent oil, pyrrolidone, and xanthan gum to prepare an emulsion with an effective ingredient concentration of 25% using conventional processes.

[0062] The petroleum ether extracts of Aconitum carmichaelii and Ginkgo biloba outer seed coat of the present invention are obtained directly from natural plants through separation and purification.

[0063] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0064] (1) Green and environmentally friendly, safe for the environment, humans, animals and natural enemies; since the main raw materials are derived from plants, they can be naturally degraded, so they have no toxic effects on the environment and no secondary pollution, which has important social significance in ensuring environmental safety.

[0065] (2) The insecticidal effect is rapid and effective. Using petroleum ether extracts of Aconitum carmichaelii and Ginkgo biloba seed coat as active ingredients, it has a short contact killing time, rapid action, and long-lasting effect. It is effective against common pests such as beet armyworm, armyworm, and cotton bollworm in crops such as rice, cotton, and vegetables, with an efficacy rate of over 90%, providing a new approach for controlling these pests in agricultural production.

[0066] (3) The raw materials are abundant, the preparation method is simple, and it has great economic significance: the obtained petroleum ether extracts of Aconitum carmichaelii and Ginkgo biloba seed coats have good insecticidal activity when compounded.

[0067] (4) It is not easy to develop resistance to pesticides. It can be mixed with other biological or chemical pesticides to enhance the effect. Due to the diversity of plant extract components, it is difficult for pests to develop resistance to pesticides.

[0068] In summary, this invention provides a composition that can be used as an insecticide, comprising aconite petroleum ether extract and ginkgo petroleum ether extract. Research in this invention has found that the combination of aconite petroleum ether extract and ginkgo petroleum ether extract in a certain mass ratio exhibits excellent insecticidal activity against lepidopteran insects, especially against common field crop pests (including beet armyworm, armyworm, and cotton bollworm), achieving a kill rate of over 90%. Compared with using aconite petroleum ether extract or ginkgo petroleum ether extract alone, it demonstrates a synergistic insecticidal effect. Furthermore, the insecticide composition of this invention is derived from plants, falling into the category of green and environmentally friendly plant-based pesticides. It is easily degradable, environmentally friendly, and safe for humans, animals, and the environment. Moreover, the diverse extract components of the insecticide composition of this invention can effectively prevent the development of insect resistance. Therefore, the composition of aconite petroleum ether extract and ginkgo petroleum ether extract of this invention, as an agricultural insecticide, is safe to use and provides rapid, potent, and stable insecticidal effects against pests. In addition, the preparation method of the composition of the present invention is simple, easy to operate, and highly efficient, making it suitable for industrial production. It can be developed as a novel plant-derived pesticide with rapid action, excellent insecticidal effect, high economic benefits, and diversified active ingredients.

[0069] Obviously, based on the above description of the present invention, and according to common technical knowledge and conventional methods in the field, various other modifications, substitutions or alterations can be made without departing from the basic technical concept of the present invention.

[0070] The following detailed embodiments further illustrate the above-described content of the present invention. However, this should not be construed as limiting the scope of the present invention to the following examples. All technologies implemented based on the above-described content of the present invention fall within the scope of the present invention. Detailed Implementation

[0071] Unless otherwise specified, the reagents, methods, and equipment used in the specific embodiments of this invention are all conventional reagents, methods, and equipment in this technical field. Unless otherwise specified, the reagents and materials used in the following examples are all commercially available products.

[0072] Example 1: Preparation of aconite petroleum ether extract using aconite as raw material according to the present invention.

[0073] After crushing Aconitum carmichaelii, add an ethanol-water solution (ethanol to water volume ratio of 9:1) at a material-to-liquid ratio of 1g:10-15mL and extract ultrasonically for 1 hour. After ultrasonic extraction, soak at room temperature for 48 hours, and repeat the extraction 3 times. Filter to remove residue, combine the extracts, concentrate to extract paste, dissolve the extract in water, add 2% hydrochloric acid to adjust the pH to 2-3, and extract 4 times with petroleum ether at a water:petroleum ether volume ratio of 1:2. The upper layer is the petroleum ether phase and the lower layer is the aqueous phase. Separate the layers, concentrate the petroleum ether phase to extract paste, and obtain Aconitum carmichaelii petroleum ether extract.

[0074] Example 2: The present invention prepares Ginkgo petroleum ether extract using Ginkgo biloba outer seed coat as raw material.

[0075] Collect mature, fallen ginkgo fruits, remove the outer seed coat, add an ethanol-water solution (ethanol to water volume ratio of 9:1) at a material-to-liquid ratio of 1g:10-15mL, crush the fruit, and extract by ultrasonication for 1 hour. After ultrasonication, extract at room temperature for 48 hours, for a total of 3 extractions. Filter to remove residue, combine the extracts, concentrate by rotary evaporation, dissolve in water, and extract 4 times with petroleum ether at a water:petroleum ether volume ratio of 1:2. The upper layer is the petroleum ether phase, and the lower layer is the aqueous phase. Separate the layers, concentrate the petroleum ether phase to obtain a paste, which is the ginkgo petroleum ether extract.

[0076] Example 3: The composition of Aconitum carmichaelii petroleum ether extract and Ginkgo biloba petroleum ether extract of the present invention

[0077] The preparation method of the composition of Aconitum carmichaelii petroleum ether extract and Ginkgo biloba petroleum ether extract of the present invention is as follows:

[0078] Weigh the aconite petroleum ether extract prepared in Example 1 and the ginkgo petroleum ether extract prepared in Example 2, and mix them at mass ratios of 1:5, 1:1, and 5:1 respectively to obtain three different compositions.

[0079] The following specific experimental examples demonstrate the beneficial effects of the present invention.

[0080] Experimental Example 1: Contact toxicity of a combination of Aconitum carmichaelii petroleum ether extract and Ginkgo biloba petroleum ether extract as active ingredients against agricultural pests.

[0081] 1. Experimental Methods

[0082] (1) Test insects: In this experiment, the test insects were collected from the field population in the 3rd to 6th instar larvae and were raised in an artificial climate chamber (25±1℃, L / D=14 / 10, RH75%) with artificial feed until pupation and egg laying. The larvae were hatched and raised to the 3rd instar, and healthy larvae of uniform size were selected for activity testing.

[0083] (2) Preparation of test solutions of aconite petroleum ether extract and ginkgo petroleum ether extract: The aconite petroleum ether extract and ginkgo petroleum ether extract prepared in Examples 1 and 2 were accurately weighed according to the mass ratios of 1:5, 1:1 and 5:1 respectively, and dissolved in dimethyl sulfoxide to prepare three test solutions of 15 mg / mL.

[0084] (3) Contact toxicity test: Healthy 3rd instar larvae of uniform size, raised in an artificial climate chamber, were placed in clean petri dishes. 4 mL of the test solution containing the combination of Aconitum carmichaelii petroleum ether extract and Ginkgo biloba petroleum ether extract was transferred. The pests were placed in the test solution for 5 seconds, then removed. Excess solution was blotted dry on clean filter paper, and the pests were placed in clean petri dishes and fed with artificial feed. A 1:1 (volume ratio) solution of dimethyl sulfoxide and 2% Tween-80 was used as a blank control, cyhalothrin as a positive control (dissolved in dimethyl sulfoxide at a concentration of 15 mg / mL), and Aconitum carmichaelii petroleum ether extract and Ginkgo biloba petroleum ether extract alone were used as drug controls (dissolved in dimethyl sulfoxide at a concentration of 15 mg / mL). The dosage for each control group was the same as that for the test solution group (4 mL). 20 larvae were treated in each group, with 3 replicates. The number of dead insects was recorded at 10 min, 0.5 h, 12 h, 24 h, and 48 h. The criterion for determining mortality was that the insects did not move when gently touched with a brush. Contact killing activity (mortality rate) was calculated using the following formula:

[0085] Mortality rate (%) = (Morality rate in the treatment group - Mortality rate in the control group) / (1 - Mortality rate in the control group) × 100%

[0086] 2. Experimental Results

[0087] The insecticidal effects of different insecticides on different insect species are shown in Table 1.

[0088] Table 1. Mortality rates of different test insects at 15 mg / ml concentrations in the tested and control groups.

[0089]

[0090] The contact toxicity test results in Table 1 show that the combination of Aconitum carmichaelii petroleum ether extract and Ginkgo biloba petroleum ether extract at a certain mass ratio significantly improved the contact toxicity against beet armyworm, armyworm, and cotton bollworm compared to using them alone. The results were comparable to the positive control (cyhalothrin). In particular, the 1:1 mass ratio of Aconitum carmichaelii petroleum ether extract and Ginkgo biloba petroleum ether extract exhibited extremely rapid knockdown effects on beet armyworm, armyworm, and cotton bollworm, indicating that the combined use of Aconitum carmichaelii petroleum ether extract and Ginkgo biloba petroleum ether extract has a good contact toxicity effect on pests. Compared with the use of each petroleum ether extract alone, it exhibits a synergistic effect, demonstrating its advantages as an agricultural insecticide: rapid action, excellent insecticidal effect, and broad insecticidal range.

[0091] Experiment Example 2: Acute toxicity test of petroleum ether extracts of Aconitum carmichaelii and Ginkgo biloba.

[0092] 1. Experimental Methods

[0093] (1) Experimental animals: Kunming mice, half male and half female, weighing between 22±2g, were provided by Chengdu Dashuo Experimental Animal Co., Ltd. The animal room temperature was maintained at 23±1℃, humidity at 55±5%, and photoperiod at 16:8h (L:D). Mice were acclimatized for 5 days prior to the experiment, with free access to water and feed. Food and water were restricted for 12 hours prior to the experiment. Animal experiments were conducted strictly in accordance with relevant national regulations for the management of experimental animals.

[0094] (2) Preparation of drug solution: Dissolve sodium carboxymethyl cellulose (CMC-Na) in physiological saline to prepare a 2.5% CMC-Na solution, and dissolve the two plant petroleum ether extracts in the CMC-Na solution to prepare the test drug solution.

[0095] (3) Acute toxicity test: Mice were randomly divided into 5 groups (100 mg / mL and 50 mg / mL of Aconitum carmichaelii petroleum ether extract, 100 mg / mL and 50 mg / mL of Ginkgo biloba petroleum ether extract, and blank control), with half males and half females, and 5 mice in each group. Each mouse was weighed. Each mouse was administered 0.5 mL of the drug by gavage. The time and number of deaths of the animals were observed and recorded after one administration, and the observation was continued for 7 days. The blank control was administered an equal volume of physiological saline by gavage.

[0096] 2. Experimental Results

[0097] Experimental results showed that in the acute toxicity test, no animals died within 7 days at the maximum dissolving dose (100 mg / mL) of the blank control, the petroleum ether extract of Aconitum carmichaelii, and the petroleum ether extract of Ginkgo biloba. This indicates that the petroleum ether extracts of Aconitum carmichaelii and Ginkgo biloba of the present invention have no acute toxicity to mice and are highly safe for use.

[0098] In summary, this invention provides a composition that can be used as an insecticide, comprising aconite petroleum ether extract and ginkgo petroleum ether extract. Research in this invention has found that the combination of aconite petroleum ether extract and ginkgo petroleum ether extract in a certain mass ratio exhibits excellent insecticidal activity against lepidopteran insects, especially against common field crop pests (including beet armyworm, armyworm, and cotton bollworm), achieving a kill rate of over 90%. Compared with using aconite petroleum ether extract or ginkgo petroleum ether extract alone, it demonstrates a synergistic insecticidal effect. Furthermore, the insecticide composition of this invention is derived from plants, falling into the category of green and environmentally friendly plant-based pesticides. It is easily degradable, environmentally friendly, and safe for humans, animals, and the environment. Moreover, the diverse extract components of the insecticide composition of this invention can effectively prevent the development of insect resistance. Therefore, the composition of aconite petroleum ether extract and ginkgo petroleum ether extract of this invention, as an agricultural insecticide, is safe to use and provides rapid, potent, and stable insecticidal effects against pests. In addition, the preparation method of the composition of the present invention is simple, easy to operate, and highly efficient, making it suitable for industrial production. It can be developed as a novel plant-derived pesticide with rapid action, excellent insecticidal effect, high economic benefits, and diversified active ingredients.

Claims

1. A plant extract composition as an insecticide, characterized by: It is composed of the following components in the following weight ratio: 1-5 parts of aconite petroleum ether extract and 1-5 parts of ginkgo petroleum ether extract; The preparation method of the Aconitum carmichaelii petroleum ether extract includes the following steps: (1) Crush the aconite root, add an ethanol aqueous solution for ultrasonic extraction, and then soak it. (2) Combine the extracts and concentrate them into an extract. Dissolve the extract in water and adjust the pH to 2-3. (3) Add petroleum ether for extraction, and concentrate the petroleum ether phase to an extract to obtain the final product; The preparation method of the Ginkgo petroleum ether extract includes the following steps: 1) Take the outer seed coat of ginkgo fruit, add an ethanol aqueous solution for ultrasonic extraction, and then soak it; 2) Combine the extracts, concentrate them, and dissolve them in water; 3) Add petroleum ether for extraction, and concentrate the petroleum ether phase to an extract to obtain the final product.

2. The plant extract composition according to claim 1, characterized by: It is composed of the following components in the following weight ratio: One part of aconite petroleum ether extract and one part of ginkgo petroleum ether extract.

3. The plant extract composition according to claim 1, characterized in that: In step (1), the ratio of Aconitum carmichaelii to ethanol aqueous solution is 1g:10~15mL; And / or, in step (1), the volume ratio of ethanol to water in the ethanol-water solution is 9:1; And / or, in step (1), the duration of the ultrasound is 1 to 5 hours; And / or, in step (1), the extraction time is 48 to 60 hours; And / or, in step (1), the extraction is performed 1 to 3 times; And / or, in step (2), the pH adjustment is performed using hydrochloric acid; And / or, in step (3), the extraction is performed 1 to 5 times.

4. The plant extract composition according to claim 1, characterized in that: In step 1), the ratio of the outer seed coat of the ginkgo fruit to the ethanol aqueous solution is 1g:10~15mL; And / or, in step 1), the volume ratio of ethanol to water in the ethanol-water solution is 9:1; And / or, in step 1), the outer seed coat is crushed before the ultrasonic extraction; And / or, in step 1), the ultrasound duration is 1 to 5 hours; And / or, in step 1), the extraction time is 48 to 60 hours; And / or, in step 1), the extraction is performed 1 to 3 times; And / or, in step 3), the extraction is performed 1 to 5 times.

5. A method for preparing the plant extract composition according to any one of claims 1 to 4, characterized in that: It includes the following steps: Weigh out the petroleum ether extract of Aconitum carmichaelii and the petroleum ether extract of Ginkgo biloba according to the specified weight ratio, mix them, and the product is obtained.

6. Use of the plant extract composition according to any one of claims 1 to 4 in the preparation of an insecticide; wherein the insecticide is an insecticide for killing insects; and wherein the insects are beet armyworm, cotton bollworm, and armyworm.

7. An insecticide, characterized in that: It is a formulation prepared by using the plant extract composition according to any one of claims 1 to 4 as the active ingredient, plus excipients or auxiliary ingredients acceptable in the field of pesticide formulation; or, it is a formulation prepared by using the plant extract composition according to any one of claims 1 to 4 and other pesticides as active ingredients, plus excipients or auxiliary ingredients acceptable in the field of pesticide formulation.

8. The insecticide according to claim 7, characterized in that: The excipients or auxiliary ingredients acceptable in the field of pesticide formulations are pesticide adjuvants.

9. The insecticide according to claim 8, characterized in that: The pesticide adjuvant is an excipient; And / or, the formulation is a dry powder, paste, solution, emulsifiable concentrate, suspension, water-in-oil emulsion, wettable powder, microemulsion or granules; And / or, the other pesticides are organophosphate pesticides, pyrethroid pesticides, carbamate pesticides, or plant-derived pesticides.

Images